Rathore R.,UP Pt Deen Dayal Upadhyay |
Rahal A.,UP Pt Deen Dayal Upadhyay |
Mandil R.,UP Pt Deen Dayal Upadhyay |
Prakash A.,UP Pt Deen Dayal Upadhyay
Asian Journal of Animal and Veterinary Advances | Year: 2014
Mimosa pudica is an indigenous antifertility herb. Its use has been advocated against urinary complaints, hypertension, pain relief and menorrhagia but the use has not been scientifically validated till date. Present study was undertaken to delineate the molecular mechanism of tocolytic activity of Hot Methanolic Extract of Mimosa pudica Seeds (HMEMPS) on isolated uterine strips of pregnant buffaloes. Myometrial strips were prepared, mounted in an organ bath containing Ringer Lock's solution maintained at 37°C and responses recorded using a physiograph in the presence and absence of different antagonists. M. pudica seeds extract produced concentration-dependent inhibitory effect on buffalo myometrium with an EC50 value of 222.4 μg mL-1 perhaps through inhibitory β receptors. Following propranolol (10-6 M) pretreatment, there was an apparent and marked increase in amplitude of spikes of normal spontaneity. An apparent increase in amplitude of spikes upto 1000 μg mL-1 followed by a decrease with further increase in concentration of HMEMPS was also recorded. Verapamil (10-12 M), a Ca+2-channel blocker, apparently could not significantly alter the HMEMPS-induced inhibiton on buffalo myometrium. Calcium channels did not seem to regulate tocolytic effect of Mimosa pudica seeds extract. Further studies are indicated to explore the involvement of Ca2+ and K+ channels, NO and other signaling mechanisms including second messengers. © 2014 Academic Journals Inc.