UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan

Mathura, India

UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan

Mathura, India

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Sudan V.,U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | Jaiswal A.K.,U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | Parashar R.,U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | Shanker D.,U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan
Indian Journal of Animal Sciences | Year: 2014

A nested polymerase chain reaction (nPCR)-based assay was developed and evaluated for rapid detection of latent and cryptic cases of Trypanosoma evansi in buffaloes. Four oligonucleotide primers (TE1, TE2, TE3 and TE4), selected from nuclear repetitive gene of T. evansi, were designed and used for PCR amplifications. The first amplification, using a pair of outer primers TE1 and TE2, produced a 821-bp primary PCR product from T. evansi DNA. The second amplification, using nested (internal) pair of primers TE3 and TE4, produced a 270-bp PCR product. The nested primers TE3 and TE4 increased the sensitivity of the PCR assay and as little as 10 fg of T. evansi DNA (equivalent to a single copy of the putative gene of the parasite) was amplified and visualized onto ethidium bromide-stained agarose gels. Amplification products were not detected when the PCR-based assay was applied to DNA from other blood parasites including Thieleria annulata and Babesia bigemina. The described nPCR-based assay provides a valuable tool to study the epidemiology of T. evansi infection in buffaloes and other susceptible animal populations.


Gangwar C.,UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | Kumar R.,UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | Singh S.P.,UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | Singh S.K.,UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | And 2 more authors.
Indian Journal of Animal Sciences | Year: 2015

Blood samples were collected from 36 pregnant buffaloes in third trimester of pregnancy. Out of total, 24 were suffering with pre partum vaginal prolapse (affected group) while the remaining 12 were normal pregnant buffaloes (control group). Serum was separated from blood and serum calcium, phosphorus, magnesium and other metabolic panels like protein, glucose, triglyceride, cholesterol and urea were estimated to study their relationship during cervico-vaginal prolapse. Mean serum calcium (mg/dl) concentration in affected group was significantly lower as compared to control buffaloes. Serum phosphorus and magnesium levels did not differ significantly between 2 groups. Serum glucose level (mg/dl) was significantly higher in affected group as compared to the control animals. Total protein (mg/dl) was significantly lower in prolapsed buffaloes as compared to healthy buffaloes. No significant difference of serum urea and cholesterol was found between affected and control groups while serum triglycerides (mg/dl) was significantly higher in prolapsed buffaloes (45.25) as compared to control group. © 2015, Indian Council of Agricultural Research. All rights reserved.

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