UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan

Mathura, India

UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan

Mathura, India
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Sudan V.,U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | Jaiswal A.K.,U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | Parashar R.,U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | Shanker D.,U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan
Indian Journal of Animal Sciences | Year: 2014

A nested polymerase chain reaction (nPCR)-based assay was developed and evaluated for rapid detection of latent and cryptic cases of Trypanosoma evansi in buffaloes. Four oligonucleotide primers (TE1, TE2, TE3 and TE4), selected from nuclear repetitive gene of T. evansi, were designed and used for PCR amplifications. The first amplification, using a pair of outer primers TE1 and TE2, produced a 821-bp primary PCR product from T. evansi DNA. The second amplification, using nested (internal) pair of primers TE3 and TE4, produced a 270-bp PCR product. The nested primers TE3 and TE4 increased the sensitivity of the PCR assay and as little as 10 fg of T. evansi DNA (equivalent to a single copy of the putative gene of the parasite) was amplified and visualized onto ethidium bromide-stained agarose gels. Amplification products were not detected when the PCR-based assay was applied to DNA from other blood parasites including Thieleria annulata and Babesia bigemina. The described nPCR-based assay provides a valuable tool to study the epidemiology of T. evansi infection in buffaloes and other susceptible animal populations.


Nakade U.P.,U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | Sharma A.,U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | Choudhury S.,U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | Bhatiya S.,U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | And 4 more authors.
Indian Journal of Animal Sciences | Year: 2017

Lead produced dose-dependent contractile effect on buffalo myometrium and it was more marked on tonicity and amplitude while almost negligible on phasic contractions and frequency. In the presence of Ca2+-free Ringer Locke solution (RLS) and nifedipine, lead-induced uterotonic effect was significantly attenuated with decrease in Emax thus, suggesting major role of extracellular Ca2+ in uterotonic effect of lead and involvement of L-type Ca2+- channels in mediating this response. Direct excitatory effect of cyclopiazonic acid (CPA) on myometrium indicates possible Ca2+-influx via store operated calcium channels (SOCC). Lead failed to evoke any excitatory effect in the concurrent presence of nifedipine (1μM) and CPA (10μM), thus intracellular calcium seems to have negligible role in lead-induced myometrial contraction. In Ca2+-free high K+ (80 mM) depolarizing solution, lead-induced excitatory effect was attenuated by GF 109203X; which suggests permeation of lead through L-type Ca2+ channels to replace Ca2+ which directly activates protein kinase C (PKC) to produce excitatory effect. Thus lead seems to interact with calcium in buffalo myometrium in a dynamic fashion and exerts calcium-depending and calcium-mimicking effects; and therefore, lead is likely to have adverse effect on reproductive functions in buffaloes.


Gangwar C.,UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | Kumar R.,UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | Singh S.P.,UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | Singh S.K.,UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | And 2 more authors.
Indian Journal of Animal Sciences | Year: 2015

Blood samples were collected from 36 pregnant buffaloes in third trimester of pregnancy. Out of total, 24 were suffering with pre partum vaginal prolapse (affected group) while the remaining 12 were normal pregnant buffaloes (control group). Serum was separated from blood and serum calcium, phosphorus, magnesium and other metabolic panels like protein, glucose, triglyceride, cholesterol and urea were estimated to study their relationship during cervico-vaginal prolapse. Mean serum calcium (mg/dl) concentration in affected group was significantly lower as compared to control buffaloes. Serum phosphorus and magnesium levels did not differ significantly between 2 groups. Serum glucose level (mg/dl) was significantly higher in affected group as compared to the control animals. Total protein (mg/dl) was significantly lower in prolapsed buffaloes as compared to healthy buffaloes. No significant difference of serum urea and cholesterol was found between affected and control groups while serum triglycerides (mg/dl) was significantly higher in prolapsed buffaloes (45.25) as compared to control group. © 2015, Indian Council of Agricultural Research. All rights reserved.

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