Up Council Of Agricultural Research

Lucknow, India

Up Council Of Agricultural Research

Lucknow, India
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Kumar V.,Subharti University | Singh S.,Sardar Vallabhbhai Patel University of Agriculture and Technology | Chandra S.,Sardar Vallabhbhai Patel University of Agriculture and Technology | Singh B.R.,Sardar Vallabhbhai Patel University of Agriculture and Technology | Yadav A.,Up Council Of Agricultural Research
Journal of Pure and Applied Microbiology | Year: 2016

The effect of green house type solar dryer and cabinet type dryer used for tomato drying with pretreatment of preservatives. We can see that the microbial growth not detected in the starting 30 days but the highest microbial count is 4.67×105 of untreated samples (6mm) in aluminum foil package under green house type solar dryer and microbial growth 4.55×105 of untreated samples (6mm) in LDPE packaging under cabinet tray dryer (65 °C). These are samples packed in LDPE and aluminum foil packaging materials and stored at room temperature. All microorganisms have a defined temperature range in which they grow, with a minimum, maximum, and optimum. An understanding of the interplay between time, temperature, and other intrinsic and extrinsic factors is crucial to selecting the proper storage conditions for a food product.

Srivastava R.,National Institute of Plant Genome Research | Upadhyaya H.D.,Indian International Crops Research Institute for the Semi Arid Tropics | Kumar R.,Up Council Of Agricultural Research | Daware A.,National Institute of Plant Genome Research | And 7 more authors.
Frontiers in Plant Science | Year: 2017

Identification of functionally relevant potential genomic loci using an economical, simpler and user-friendly genomics-assisted breeding strategy is vital for rapid genetic dissection of complex flowering time quantitative trait in chickpea. A high-throughput multiple QTL-seq strategy was employed in two inter (Cicer arietinum desi accession ICC 4958 × C reticulatum wild accession ICC 17160)- and intra (ICC 4958 × C. arietinum kabuli accession ICC 8261)-specific RIL mapping populations to identify the major QTL genomic regions governing flowering time in chickpea. The whole genome resequencing discovered 1635117 and 592486 SNPs exhibiting differentiation between early- and late-flowering mapping parents and bulks, constituted by pooling the homozygous individuals of extreme flowering time phenotypic trait from each of two aforesaid RIL populations. The multiple QTL-seq analysis using these mined SNPs in two RIL mapping populations narrowed-down two longer (907.1 kb and 1.99 Mb) major flowering time QTL genomic regions into the high-resolution shorter (757.7 kb and 1.39 Mb) QTL intervals on chickpea chromosome 4. This essentially identified regulatory as well as coding (non-synonymous/synonymous) novel SNP allelic variants from two efl1 (early flowering 1) and GI (GIGANTEA) genes regulating flowering time in chickpea. Interestingly, strong natural allelic diversity reduction (88–91%) of two known flowering genes especially mapped at major QTL intervals as compared to that of background genomic regions (where no flowering time QTLs were mapped; 61.8%) in cultivated vis-à-vis wild Cicer gene pools was evident inferring the significant impact of evolutionary bottlenecks on these loci during chickpea domestication. Higher association potential of coding non-synonymous and regulatory SNP alleles mined from efl1 (36–49%) and GI (33–42%) flowering genes for early and late flowering time differentiation among chickpea accessions was evident. The robustness and validity of two functional allelic variants-containing genes localized at major flowering time QTLs was apparent by their identification from multiple intra-/inter-specific mapping populations of chickpea. The functionally relevant molecular tags delineated can be of immense use for deciphering the natural allelic diversity-based domestication pattern of flowering time and expediting genomics-aided crop improvement to develop early flowering cultivars of chickpea. © 2017 Srivastava, Upadhyaya, Kumar, Daware, Basu, Shimray, Tripathi, Bharadwaj, Tyagi and Parida.

Kumar R.,Banaras Hindu University | Kumar R.,Up Council Of Agricultural Research | Sinha A.,Banaras Hindu University | Mahajan G.,Indian Agricultural Research Institute
Journal of Environmental Biology | Year: 2017

Aim: Soil borne phytopathogens are one of the major concern of today's agricultural system. In the present study, dominant fungal decomposers were selected and their potential as biological control agents was evaluated against some soil borne plantpathogens. Methodology: Effect of green manure amendment on the sclerotia viability of three soil-borne plant pathogens viz., Sclerotium rolfsii, Rhizoctonia solani and Sclerotinia sclerotiorum, effect of fungal decomposers on soil-borne phytopathogens in dual culture, effect of volatile and non-volatile metabolites of dominant fungal decomposers on the radial growth and sclerotia production of test pathogens were evaluated. Results: Green manure amendment resulted in reduced in sclerotia of S. rolfsii, R. solani and S. sclerotiorum by 40, 36 and 36.50%, respectively. In dual culture, the maximum growth inhibition of S. rolfsii, R. solani and S. sclerotiorum with Trichoderma harzianum were 49.95, 47.62 and 57.83%, respectively The maximum inhibition of S. rolfsii and S. sclerotiorum caused by the volatile metabolites produced by Trichoderma harzianum were 56.64 and 43.95%, whereas the maximum inhibition of R. solani was caused by volatile metabolites of Penicillium citrinum (44.96%). The maximum inhibition of S. rolfsii, R. solani and S. sclerotiorum through non-volatile metabolites of Trichoderma harzianum were 51.04,57.30 and 49.10%, respectively. The maximum reduction in sclerotia of S. rolfsii, R. solani and S. sclerotiorum with Trichoderma harzianum were 86.44, 88.54 and 88.20% respectively, under dual culture after 21 days of incubation. The maximum reduction in sclerotia of S. rolfsii, R. solani and S. sclerotiorum with Trichoderma harzianum were 95.42, 93.60 and 91.32%, respectively, under the effect of volatile metabolites after 21 days of incubation. The maximum reduction in sclerotia of S. rolfsii, R. solani and S. sclerotiorum with Trichoderma harzianum were 87.46, 79.74 and 85.10% respectively, under the effect of non-volatile metabolites after 21 days of incubation. Interpretation: All eight fungal decomposers effectively contribute in controlling the soil borne phytopathogens. Overall T harzianum, Aspergillus niger and Penicillium citrinum proved as potential bio-control agents against all soil borne plant pathogens viz., S. rolfsii, R. solani and S. sclerotiorum. © Triveni Enterprises, Lucknow (India).

Shalaby S.I.,National Research Center of Egypt | Awad M.M.,Suez Canal University | El Dean H.N.,Suez Canal University | Mohamed R.A.,Suez Canal University | And 2 more authors.
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2016

It is particularly important for aged people to adopt diet and lifestyle habits that minimize the risk of morbidity and maximize the prospects for healthy aging. The aim of this study was to improve elderly nutritional knowledge, attitude, and practice by conducting quasi-experimental intervention study through assessment of nutritional knowledge, practice and attitude of elderly people (n=115, +60 years) in Abu Khalifa village, Ismailia, Egypt, implementation of a nutritional health education program for them and evaluation of the effect of the program based on an interview questionnaire form. Our results showed that the total knowledge increased from a pre-intervention level of 26.1% to a post-level of 82.6% (p < 0.001). Total positive attitude increased from a pre-intervention level of 28.7% to a post-level of 60.0% (p < 0.001). The attitude was positively correlated with the health perception at the pre-phase, and negatively to the crowding index and the number of diseases at the post-phase. The statistically significant independent predictors of the knowledge scores were intervention and the level of education, income, and health perception, all being positive and of attitude score, income, number of chronic diseases, physical activity, and knowledge score, all being negative except for the knowledge score. © 2010 RJPBCS.

Kumar H.,Indian Veterinary Research Institute | Bhooshan N.,Indian Veterinary Research Institute | Bhooshan N.,UP Council of Agricultural Research | Patra M.K.,Indian Veterinary Research Institute | Yadav M.C.,Indian Veterinary Research Institute
Indian Journal of Animal Sciences | Year: 2010

The trial was conducted under field conditions to study the effectiveness of a treatment with, progestagen in combination with PMSG on estrus induction, prevention of prolonged anestrus and fertility in noncycling buffaloes. Non cycling buffaloes (72) with average of 120 ± 17.6 days postpartum were randomly allotted to one of the following treatments: progestagen + PMSG (n =44), 250 mg of P Depot im daily for 5 days and 500 iu folligon on seventh day of initiation of treatment and control group (CG, n = 28) without hormonal treatment. The progestagen + PMSG group showed higher estrus and synchronization rates (59.1 and 36.4%) than CG (14.28 and 3.5%). Buffaloes receiving progestagen + PMSG had higher overall pregnancy rate than CG (45.5 vs 14.28%, respectively). The treatment-first service period and calving-conception interval was shorter in hormonal treated (8.8 ± 2.5 and 192.5 ± 16.8 days) than CG (25.2 ± 3.3 and 238.9 ± 24.7 days), respectively. In conclusion, treatment with progestagen + PMSG at 120 days postpartum was effective to induce estrus, prevent prolonged anestrus and reduce the calving-conception interval, improving reproductive performance in buffaloes.

Bhooshan N.,Up Council Of Agricultural Research | Dutta R.,IP Labs. | Singh S.,Govind Ballabh Pant Samajik Vikas Sansthan
Acta Horticulturae | Year: 2015

Intellectual property is all about monopoly in the market place and harnessing the maximum economic benefits by exercising the monopoly. Monopoly in the shape of goodwill, reputation and demand becomes the most formidable under the banner of Geographical Indication (GI) as GI is a monopoly of a guild unlike a private monopoly. Uttar Pradesh is a sacred land as the birth place of Lord Ram and Lord Krishna and has been blessed by so many unique crops and fertile land. Mango, the king of fruits, has a huge diversity in the Uttar Pradesh. Among the different mango cultivars, 'Malihabadi Dashehari' is one of the most popular mango cultivars for which GI has been secured almost three years back. Still the economic benefit of using GI is a distant dream for the entire range of people from farmers to the sellers. Monopoly is not just a Geographical Indication registration certificate secured from the Intellectual Property Office but a well-conceived business strategy to be applied in the open market place to reap rich dividends. Virtually no clue of using 'Dashehari' mango's Geographical Indication as a wealth generation tool shows lack of vision. The paper investigates into the possible reasons for non-use of 'Malihabadi Dusseheri' mango for the purpose it was registered as a GI. © 2015, International Society for Horticultural Science. All rights reserved.

Nasim J.,Ddu Gorakhpur University | Malviya N.,Ddu Gorakhpur University | Kumar R.,Up Council Of Agricultural Research | Yadav D.,Ddu Gorakhpur University
Plant Systematics and Evolution | Year: 2016

The genome mining of chickpea (Cicer arietinum L.) revealed a total of 37 putative Dof genes using NCBI BLAST search against the genome with a highly conserved Dof domain. The translated Dof proteins possessed 150–493 amino acid residues with molecular weight ranging from 16.9 to 54.4 kD and pI varied from 4.98 to 9.64 as revealed by ExPASy server ProtParam. The exon–intron organization showed predominance of intronless Dof genes in chickpea. The predicted Dof genes were distributed among the eight chromosomes with a maximum of 9 Dof genes present on chromosome 7 and a single Dof gene was found on chromosome 8.The predominance of segmental gene duplication as compared to tandem duplication was observed which might be the prime cause of Dof gene family expansion in chickpea. The cis-regulatory element analysis revealed the presence of light-responsive, hormone-responsive, endosperm-specific, meristem-specific and stress-responsive elements. Comprehensive phylogenetic analyses of Dof genes of chickpea with Arabidopsis, rice, soybean and pigeonpea revealed several orthologs and paralogs assisting in understanding the putative functions of CaDof genes. The functional divergence and site-specific selective pressures of chickpea Dof genes have been investigated. The bioinformatics-based genome-wide assessment of Dof gene family of chickpea attempted in the present study could be a significant step for deciphering novel Dof genes based on genome-wide expression profiling. © 2016 Springer-Verlag Wien

Tripathi V.K.,Cs Azad University Of Agriculture And Technology | Kumar S.,Up Council Of Agricultural Research | Gupta A.K.,Cs Azad University Of Agriculture And Technology
Indian Journal of Horticulture | Year: 2015

An experiment was carried out during two subsequent years, i.e., 2009-10 and 2010-11 to study the influence of Azotobacter, vermicompost on growth, flowering, yield and quality of strawberry cv. Chandler. There were nine treatments comprising two levels each of Azotobacter (6 and 7 kg/ha) and vermicompost (20 and 30 t/ ha) and their combinations along with one control, replicated thrice in randomized block design. Five kg of FYM was applied as a basal dose in all the treatments including control. All the doses of Azotobacter and vermicompost were applied at the time of planting in the field. The data of both the years of experiment were pooled and analyzed. The combined application of Azotobacter at 7 kg/ha + vermicompost at 30 t/ha significantly increased the height of plant (18.70 cm), number of leaves (61.60), crowns (6.77) and runners (4.83) per plant, whereas, maximum number of flowers (56.69), fruits set (25.87) per plant with increased duration of harvesting (66.80 days) and minimum number of days taken to produce first flower (55.17 days) and fruit set (6.19 days) with significantly more yield (322.38 g/plant) were observed with Azotobacter at 6 kg/ha + vermicompost at 30 t/ha applied plants. Plants fertilized with Azotobacter at 6 kg/ha + vermicompost at 30 t/ha also produced the berries with maximum length (4.76 cm), width (2.49 cm), weight (8.75 g), volume (5.97 cc), TSS (9.800Brix), total sugars (9.23%), ascorbic acid (54.72 mg/100 g edible portion) with minimum titratable acidity (0.50%) in comparison to other treatments under plains of central Uttar Pradesh. © 2015, Horticulture Society of India. All rights reserved.

PubMed | National Institute of Plant Genome Research NIPGR, Indian Council of Agricultural Research, Indian International Crops Research Institute for the Semi Arid Tropics, Indian Agricultural Research Institute and Up Council Of Agricultural Research
Type: | Journal: Plant science : an international journal of experimental plant biology | Year: 2016

The discovery and large-scale genotyping of informative gene-based markers is essential for rapid delineation of genes/QTLs governing stress tolerance and yield component traits in order to drive genetic enhancement in chickpea. A genome-wide 119169 and 110491 ISM (intron-spanning markers) from 23129 desi and 20386 kabuli protein-coding genes and 7454 in silico InDel (insertion-deletion) (1-45-bp)-based ILP (intron-length polymorphism) markers from 3283 genes were developed that were structurally and functionally annotated on eight chromosomes and unanchored scaffolds of chickpea. A much higher amplification efficiency (83%) and intra-specific polymorphic potential (86%) detected by these markers than that of other sequence-based genetic markers among desi and kabuli chickpea accessions was apparent even by a cost-effective agarose gel-based assay. The genome-wide physically mapped 1718 ILP markers assayed a wider level of functional genetic diversity (19-81%) and well-defined phylogenetics among domesticated chickpea accessions. The gene-derived 1424 ILP markers were anchored on a high-density (inter-marker distance: 0.65cM) desi intra-specific genetic linkage map/functional transcript map (ICC 4958ICC 2263) of chickpea. This reference genetic map identified six major genomic regions harbouring six robust QTLs mapped on five chromosomes, which explained 11-23% seed weight trait variation (7.6-10.5 LOD) in chickpea. The integration of high-resolution QTL mapping with differential expression profiling detected six including one potential serine carboxypeptidase gene with ILP markers (linked tightly to the major seed weight QTLs) exhibiting seed-specific expression as well as pronounced up-regulation especially in seeds of high (ICC 4958) as compared to low (ICC 2263) seed weight mapping parental accessions. The marker information generated in the present study was made publicly accessible through a user-friendly web-resource, Chickpea ISM-ILP Marker Database. The designing of multiple ISM and ILP markers (2-5 markers/gene) from an individual gene (transcription factor) with numerous aforementioned desirable genetic attributes can widen the user-preference to select suitable primer combination for simultaneous large-scale assaying of functional allelic variation, natural allelic diversity, molecular mapping and expression profiling of genes among chickpea accessions. This will essentially accelerate the identification of functionally relevant molecular tags regulating vital agronomic traits for genomics-assisted crop improvement by optimal resource expenses in chickpea.

PubMed | Indian Institute of Vegetable Research and Up Council Of Agricultural Research
Type: Journal Article | Journal: Journal of food science and technology | Year: 2015

This study was conducted at Indian Institute of Vegetable Research, Varanasi (I.I.V.R.), Uttar Pradesh from 2006 to 2009 for evaluating comparative physico-chemical, microbiological and sensory attributes of crystallized and Kashi petha at refrigerated storage (41C) condition up to 90days. The petha were manufactured using 2 varieties of ash gourd developed at I.I.V.R. i.e.; Kashi Dhawal and Kashi Ujwal, selected based on different parameters suitable for petha sweet preparation. The used packaging materials for storage studies were laminated pouch, low density polyethylene, high density polyethylene. The physiochemical analysis and sensory evaluation were performed at 15days interval upto 90days for storage study in both Kashi Petha and crystallized petha, while microbial observation was recorded up to 90days in crystallized petha and up to 50days Kashi Petha. Physico-chemical analysis exhibited the continuous increase in moisture (25.79-26.80% and 31.68-32.63%) and titratable acidity (0.022-0.029%) and (0.022-0.030%) and reduction in ascorbic acid content (5.50-5.00mg/100g and 5.12-4.84mg/100g), respectively in crystallized and Kashi petha. The increasing trend in microbial population was more pronounced in Kashi petha in comparison to crystallized petha. The spoilage bacteria included Bacillus spp., Staphylococcus spp. and lactic acid bacteria while Aspergillus, Penicillium and Saccharomyces were observed among fungal contaminants. The bacteria, yeast and mold were identified on the basis of cultural, morphological and biochemical characterization with respect to storage period. On the basis of the overall findings of comparative physico-chemical, microbiological and sensory evaluation, 30 and 50days of storage was considered as safe for the consumption of Kashi and crystallized petha, respectively under refrigerated environment (41C).

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