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Recife, Brazil

Povoa A.,Hospital Center Sao Joao | Xavier P.,Hospital Center Sao Joao | Calejo L.,Hospital Center Sao Joao | Soares S.,Hospital Center Sao Joao | And 6 more authors.
Reproductive BioMedicine Online | Year: 2016

Ovarian tissue cryopreservation represents a valid strategy to preserve ovarian function in patients with a high risk of premature ovarian failure. We present a case of ovarian tissue cryopreservation carried out in an 18-year-old woman after a laparotomy for left adnexal mass with left adnexectomy. Congenital absence of the right ovary was observed during surgery. To preserve fertility, rescue cryopreservation of ovarian tissue was carried out under extreme conditions (without adopting the standard published protocol, not yet available at our centre). Ten years later, transplantation of cryopreserved ovarian tissue was carried out and, shortly after it, restoration of ovarian function was confirmed. © 2015 Reproductive Healthcare Ltd. Source


Rajavelan M.,University of Madras | Ananthi S.,Up Biomedical | Padmanabhan K.,Electrical Machines
2013 International Conference on Emerging Trends in VLSI, Embedded System, Nano Electronics and Telecommunication System, ICEVENT 2013 | Year: 2013

This paper proposes and implements the PIC microcontroller based Surface Plasmon resonance analysis of bio molecular interaction. Surface Plasmon resonance [SPR] has becoming an important optical bio-sensing technology in the areas of medical, biological , biochemistry, pollution detection, aircraft and laboratory research. The SPR works on the principle of TIR, that when the stepper motor mounted laser beam moving back and forth is focused on the hypotenuse of the gold coated BK-7 prism at an angle greater than the critical angle, hence the laser beam gets reflected back and SPR is generated outward side of the gold coated surface is measured using LDR. The generation of SPR is measured in terms of Refractive index, the refractive index changes with respect to the applied sample (Dielectric loading). Hence even for a small change in molecular interaction can be found using SPR. The laboratory prototype of PIC microcontroller based SPR was characterized in terms of reflectance and intensity with respect to the incident beam. In the design of instrument PIC 16F73 microcontroller is used to control the laser movement back and forth through stepper motor to increase the fastness and accuracy. This paper explains and demonstrates the importance of PIC microcontroller in the design and control of the instrument. © 2013 IEEE. Source


Kumar D.,Up Biomedical | Gupta A.,Up Biomedical | Nath K.,University of Pennsylvania
Expert Review of Molecular Diagnostics | Year: 2016

Advances in the application of NMR spectroscopy-based metabolomic profiling of prostate cancer comprises a potential tactic for understanding the impaired biochemical pathways arising due to a disease evolvement and progression. This technique involves qualitative and quantitative estimation of plethora of small molecular weight metabolites of body fluids or tissues using state-of-the-art chemometric methods delivering an important platform for translational research from basic to clinical, to reveal the pathophysiological snapshot in a single step. This review summarizes the present arrays and recent advancements in NMR-based metabolomics and a glimpse of currently used medical imaging tactics, with their role in clinical diagnosis of prostate cancer. © 2016 Informa UK Limited, trading as Taylor & Francis Group Source


Tulachan B.,Indian Institute of Technology Kanpur | Meena S.K.,Indian Institute of Technology Kanpur | Rai R.K.,Up Biomedical | Mallick C.,Indian Institute of Technology Kanpur | And 10 more authors.
Scientific Reports | Year: 2014

Silk cocoon membrane (SCM) is an insect engineered structure. We studied the electrical properties of mulberry (Bombyx mori) and non-mulberry (Tussar, Antheraea mylitta) SCM. When dry, SCM behaves like an insulator. On absorbing moisture, it generates electrical current, which is modulated by temperature. The current flowing across the SCM is possibly ionic and protonic in nature. We exploited the electrical properties of SCM to develop simple energy harvesting devices, which could operate low power electronic systems. Based on our findings, we propose that the temperature and humidity dependent electrical properties of the SCM could find applications in battery technology, bio-sensor, humidity sensor, steam engines and waste heat management. Source


Srivastava S.,Up Biomedical | Bisht H.,CSIR - Central Electrochemical Research Institute | Sidhu O.P.,CSIR - Central Electrochemical Research Institute | Srivastava A.,CSIR - Central Electrochemical Research Institute | And 5 more authors.
Phytochemistry | Year: 2012

Amaranthus hypochondriacus L. infected with Ageratum enation virus (AEV) was investigated for identifying alteration in the anatomical structures, sap translocation and metabolomic variations using light microscopy, magnetic resonance imaging, NMR spectroscopy and GC-MS, respectively. Combination of GC-MS and NMR spectroscopy identified 68 polar and non-polar metabolites that were present in different levels in healthy and virus-infected A. hypochondriacus. Contrast of T 1 and T 2 weighted MR images showed significant differences in the spatial distribution of water, lipids and macromolecules indicating alterations in the cortical region and disruption of vascular bundles in virus-infected stem tissues. MRI observations are supported by light microscopic examination. Microscopic examination of AEV infected stem revealed severe hyperplasia with a considerable reduction in size of stem cells. The NMR spectroscopy and GC-MS analysis indicated that viral infection significantly affected the plant primary and secondary metabolism resulting in decreased glucose and sucrose content and increase in the concentration of β-sitosterol and stigmasterol. Higher accumulation of TCA cycle intermediates such as citric acid and malic acid in AEV infected plants indicated enhanced rate of respiratory metabolism. The viral stress significantly increases the concentration of erythritol and myo-inositol as compared to healthy ones. Lower concentration of glucose and sucrose in viral-infected stem tissues suggests decreased translocation of photosynthates in the plants. The results demonstrated potential of MRI, NMR spectroscopy and GC-MS for studying anatomical and metabolic variations in virus-infected plants. © 2012 Elsevier Ltd. All rights reserved. Source

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