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Presidente Prudente, Brazil

Gakiya H.H.,UNOESTE | Stevanin H.,Scholl of Veterinary Medicine | Cassu R.N.,Scholl of Veterinary Medicine
Acta Cirurgica Brasileira | Year: 2011

Propose: To compare the postoperative analgesic effects of electroacupuncture, morphine or sham acupuncture in dogs undergoing mastectomy. Methods: Thirty client-owed dogs undergoing to mastectomy were randomly assigned to three groups of 10 animals each and received either morphine (T-M), the electroacupuncture (T-EA) or sham procedure (T-Sham). Pre-anesthetic medication was acepromazine (0.05 mg kg-1, IM). Anesthesia was induced with propofol (4 to 5 mg kg-1, IV) and maintained with isoflurane. Postoperatively pain degree was assessed using a numerical rating scale. Dogs were scored at 1, 3, 6 and 12 hours post-extubation. If the pain score was ≥6, supplemental morphine was administered. Serum cortisol concentration was measured before pre-anesthetic medication, at 45 minutes after the anesthetic induction, and at 1, 3 and 6 hours post-extubation. Result s: The pain score did not differ among the treatments, but rescue analgesia was lower in the T-EA group (2 of 10 dogs), when compared with T-Sham (6 of 10 dogs) and T-M (6 of 10 dogs) groups. Serum cortisol concentration did not differ among the treatments. Conclusion: Electroacupuncture reduces the postoperative analgesic requirement and promotes satisfactory analgesia in dogs undergoing mastectomy. Source


Purpose: To compare the postoperative analgesic effects of the extradural tramadol or morphine in female dogs undergoing ovariohysterectomy. Methods: Sixteen female dogs were randomly assigned to two groups of eight animals each and received morphine (0.1mg kg-1 M group) or tramadol (2mg kg-1 T group). The pre-anesthetic medication was intravenously (iv) acepromazine (0.05mg kg-1). Anesthesia was induced with propofol (4mg kg-1iv) and maintained with isofurane. The degree of analgesia was evaluated using a numerical rating scale that included physiologic and behavior variables. Dogs were scored at one, three, six and 12 hours after surgery by one blinded observer. Dogs were treated with morphine (0.5mg kg-1) if their scores were >6. Serum cortisol was measured before the pre-anesthetic medication was administered (basal), at the time of the ovarian pedicle clamping (T0), and at 1 (T1), 6 (T6) and 12 (T12) hours postoperative. Results: The pain score did not differ between morphine and tramadol treatments. Rescue analgesia was administered to one dog in the T treatment group. Serum cortisol did not differ between treatments. Conclusion: The extradural administration of morphine or tramadol is a safe and effective method of inducing analgesia in female dogs undergoing ovariohyterectomy. Source


A study was conducted to investigate whether the rhizosphere of pastures promotes root colonization by rhizobacteria of the Bacillus genus, as also the fertility of this soil interferes with bacterial colonization. For this we collected samples of the root system of B. decumbens, B. brizantha and Panicum maximum in 18 farms with cattle ranching in western Sao Paulo, Brazil. While driving to work were effective analysis of soil fertility in the sampled areas. Quantification of Bacillus spp. In the rhizosphere of plants was performed by employing the technique of thermal shock and dilution ending with plating on culture media. Colonization of Bacillus in the rhizosphere of pasture were considered low and there was no correlation between rhizosphere colonization with most of the parameters of soil fertility. Just organic matter in the soil correlated negatively with bacterial colonization in the rhizosphere. No significant difference was found in the colonization of Bacillus in the rhizosphere of three grassland species assessed. Source


Neto N.B.M.,UNOESTE | Moryia A.T.,UNOESTE
Crop Breeding and Applied Biotechnology | Year: 2010

Macadamia is one of the most valuable commercial nuts and its culture is a good alternative for small farmers or for large properties with a low capacity for the mechanization of its areas. Macadamia clones, due to their morphological proximity exhibited some difficulties in field identification that could difficult the right plant distribution. The objective of this work was to genetically characterize, by RAPD markers, different macadamia clones under cultivation. Leaves from 'HAES-741', 'HAES-660', 'IAC-920BX', 'IAC-920', 'HAES-344', 'IAC-420', 'HAES-816', 'IAC-1.21', 'Shimada', 'IAC - Campinas B', 'IAC - Campinas A' and 'HAES-Aloha' were donated by Queen Nut Macadâmia. DNA was extracted and amplified with 15 primers and the binary data (presence/absence of bands) collected was used and dendrograms constructed. Clone identification, via RAPD was possible using primers Op G18, C19 and C7. Most of the genetic variation is inside the Brazilian or Hawaiian groups rather than between the groups and could allow inbreeding or crossing between distant clones. Source


Disruptions in sperm production include decreased sperm motility and increased of abnormal sperm. Seminal plasma appears to exert important effects on sperm function. The objective was to evaluate the dry and rainy season influence on the seminal characteristics and semen plasma proteins. Eleven bulls with ages ranging from 34 to 38 months were submitted each one to 12 semen collect with eletroejaculation 6 on dry season and 6 on rainy season with 14 days interval, totalizing 144 samples. Qualitative and quantitative semen characteristics were evaluated. Samples of semen were centrifuged (1.500 g / 15 minutes) and conditioned and stored (-20°C) until further processing. The proteins were extracted and quantified to electrophoresis performed. Variance analysis and Tukey test 5% was used. The semen vigor (P < 0.01), minor defects and total defects (P < 0.05) showed statistical difference between seasons, while the volume, motility and minor defects did not (P > 0.05). The number of bands occurred between 6 and 125 kDa. The molecular band of 26 kDa was present in 100% of bulls in rainy season. The molecular bands of 6, 9 and 125 kDa showed a high frequency in dry and rainy season. In conclusion, these results showed a band distribution variation throughout the season and the year seasons changed the semen quality with increase sperm vigor and reduction of abnormal sperm on dry season. Source

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