Pocco M.E.,CONICET |
Damborsky M.P.,CONICET |
Animal Biodiversity and Conservation | Year: 2010
Species diversity and abundance of Orthoptera communities were estimated in two grasslands of Eastern Humid Chaco from 2006 to 2007. The greatest species diversity was recorded in the grassland with intensive livestock grazing and predominance of native plant species, whereas the abundance values were higher in the grassland without grazing and characterized by introduced plant species. In total, 25 Orthoptera species were collected during the study. Acrididae (Caelifera) was the most abundant and diverse family recorded. Staurorhectus longicornis, Dichromorpha australis and Orphulella punctata were the most abundant species; the last two species being recorded during the entire sampling period. The greatest abundance was recorded in December 2006. Abundance did not show any correlation with climatic variables. © 2010 Museu de Ciències Naturals.
Rebak G.I.,FCV |
Capellari A.,FCV |
Ynsaurralde Rivolta E.A.,UNNE
Revista Veterinaria | Year: 2010
The buffalo population in the country is approximately 100,000 heads located in the northeastern provinces of Argentina, and there is a growing need of bulls selected for production systems. To improve the fertility of bulls is important to make use of reproductive characteristics of medium to high heritability and genetic correlations among themselves favorable for the production system, such as those related to growth. Within reproductive parameters, scrotal circumference (SC) is a feature to be included in breeding programs for beef bulls to be genetically linked, so favorable to the semen quality and reproductive precocity in females. Testicular development measured by scrotal circumference increases with growth and is correlated with body weight. The objectives of the study were to evaluate the buffalo bulls testicular development in post-weaning and correlate with bodyweight. The trial was conducted in Ita Ibaté; General Paz Department of the Province of Corrientes. The main activities include cattle and meat buffalo's production, with 1600 acres of of low-field for buffalos production. The animals used were bulls buffalos, Murrah crosses by Mediterranean. The animals come from an extensive system of service fall, autumn calving and weaning of spring. Feeding the animals were subjected to continuous grazing field was natural and the health plan used was recommended for cattle. Measurements in animals were: weight, height at the hips, gasping and measuring scrotal circumference at weaning and year and a half years. The scrotal circumference is positively correlated with body weight.
Candia O.A.,Mount Sinai School of Medicine |
Gerometta R.M.,UNNE |
Danias J.,SUNY Downstate Medical Center
Experimental Eye Research | Year: 2014
We have previously shown that tissue plasminogen activator (tPA) injected in the vitreous of sheep, reduced or prevented the elevation of the intraocular pressure (IOP) normally produced by the instillation of 1% prednisolone. We now report the effect of tPA when injected into the anterior chamber (AC) in amounts of 0.01, 0.001 and 0.0001μg diluted in a volume of 50μL. Lyophilized tPA, obtained as Actilyse® 50mg from Boehringer Ingelheim containing arginine was utilized. The Actilyse was diluted in balanced salt solution to obtain the desired amount of tPA in 50μL. An identical solution containing only arginine was prepared to inject into the contralateral eye as a control. Six sheep of the Corriedale breed were selected. At the beginning of the study all eyes received instillation of 1% prednisolone 3times/day for 10days to elevate their IOP from 10mmHg to about 23mmHg. Then, 0.0001μg was injected into one of the eyes and its effect was followed for up to 55:00h while the instillation of prednisolone continued in both eyes. The same protocol was implemented for the 0.001 and 0.01μg amounts after extended washout and IOP was over 22mmHg. The injection of 0.0001μg into the AC had no effect on an IOP of 23.0mmHg at 6:00 and 30:00h after injection. 0.001μg of tPA reduced IOP from 23.1 to 18.6mmHg at 6:00h but IOP recovered to 22.3mmHg at 30:00h. Injection of 0.01μg produced a marked and prolonged reduction of IOP. From a baseline of 23.0, IOP was reduced to 14.0, 14.7, 21.2, and 20.9mmHg at 5.0, 23.0, 27.0 and 45.5h, respectively. The 0.423μg of arginine, which is associated with 0.01μg tPA, was injected alone and had no effect. Recombinant human tPA injected in the AC is effective in reversing steroid-induced IOP elevation in sheep. The reduction of IOP elevation may be the result of an effect on extra-cellular matrix turnover in the TM. These findings suggest that tPA may by useful as a therapeutic agent in steroid-induced glaucomas. © 2014 Published by Elsevier Ltd.
Plant regeneration of tea (Camellia sinensis) by in vitro culture of meristems, axillary buds and uninodal segments [Regeneración de plantas de té (Camellia sinensis) por cultivo in vitro de meristemas, yemas axilares y segmentos uninodales]
Molina S.P.,Instituto Nacional de Tecnologia Agropecuaria |
Perez M.L.,UNNE |
Rey H.Y.,Northeast National University |
Mroginski L.A.,Northeast National University
Revista de la Facultad de Ciencias Agrarias | Year: 2013
Plants of two clones (CH 14 INTA and CH 318 INTA) of tea (Camellia sinensis (L.) O. Kuntze) were regenerated by in vitro culture of three types of explants disinfected by immersion in 70% ethanol (1 min) and 1.5% sodium hypochlorite (20 min). The best medium for shoot regeneration from uninodal segments, for both clones, as well as for axillary buds of CH 14 INTA clone was 1/2 MS + 1 mg/L BAP. While the best medium for axillary buds of CH 318 clone was 1/2 MS + 1 mg/L BAP + 1 mg/L AG3 For meristems culture, the best medium, for both clones was 1/2 MS + 1 mg/L KIN + 1 mg/L AG3. Rooting of regenerated shoots were achieved by culture them on 1/4 MS + 6 mg/L IBA.
Gerometta R.,UNNE |
Gerometta R.,Mount Sinai School of Medicine |
Kumar S.,SUNY Downstate Medical Center |
Shah S.,SUNY Downstate Medical Center |
And 3 more authors.
Investigative Ophthalmology and Visual Science | Year: 2013
Purpose. To investigate whether tissue plasminogen activator (tPA) can prevent and/or reverse steroid-induced IOP elevation in an ovine model. Methods. Three animal groups were subjected to bilateral steroid-induced IOP elevation using thrice daily topical ocular prednisolone administration. In the first group (N = 8), one eye each of two sheep was injected intravitreally with 100 μg, 200 μg, 500 μg, or 1 mg human recombinant tPA, while contralateral eyes received vehicle. In the second group (N = 2), one eye was injected intravitreally with tPA (100 μg), while contralateral eyes received vehicle containing L-arginine. In the third group (N = 4), each animal received intravitreal tPA in one eye concurrently with initiation of bilateral steroid administration. IOP was monitored for the duration of the experiment. Tissues from eyes of the third group were used to determine relative gene expression. Results. In the first and second groups, IOP decreased by 9.7 (±2.8) and 9.7 (±1.6) mm Hg, respectively, 24 hours after tPA administration. In the third group, tPA-treated eyes did not develop IOP elevation with ΔIOP of 11.8 (±1.3) mm Hg 8 days later. In all tPA-treated eyes, IOP remained low until the end of the study. mRNA levels in the trabecular meshwork were decreased for plasminogen activator tissue (PLAT), increased for matrix-metalloproteinase 1 (MMP-1), and stable for plasminogen activator inhibitor 1 (PAI-1), MMP-2, MMP-9, and MMP-13 in tPA-treated eyes compared with contralateral controls. PAI-1 mRNA levels in ciliary processes also remained similar. Conclusions. Recombinant human tPA is effective in both preventing and reversing steroid-induced IOP elevation in sheep. Tissue plasminogen activator may be useful as a therapeutic agent in steroid-induced glaucoma. © 2013 The Association for Research in Vision and Ophthalmology, Inc.