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Corrientes, Argentina
Corrientes, Argentina
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Pantiu A.J.,Northeast National University | Capellari A.,Northeast National University | Gimenez L.I.,UNNE
Zootecnia Tropical | Year: 2015

The aim was to compare two treatments strategic permanent vs winter strategic suplementation on variables of body and reproductive growth heifer rearing up to the weight of weanign in a forestry livestock system. The experimental design was completely randomized with two treatments and four repetitions. In treatments 1 (T1): permanent supplementation, the animals received daily ration of maize and cotton expeller 0.83% live weight, grazing Axonopus, during the 9 months of the trial. While in treatments 1 (T2): Winter strategic supplementation, the ration was administered for 4 months (winter), the remaining period only consumed pasture. Every 30 days daily live weight (PV), total gain (GT) and weight (GDP), height at the withers (AC) and croup (AG), body length (LC), thoracic perimeter (PT) was taken; at the end of the experience, we proceeded to grade reproductive development measurement (GRD). The data were analyzed in a context of general and linear mixed models for repeated measures over time and for variable GRD Kruskal Wallis, nonparametric test was performed. The results yielded no signifi cant differences between treatments being PV 274.2±5.8 and 262±5.8Kg, GT 112.5±5.4 and 101.2±5.2Kg, GDP 0.46±0.03 and 0.41±0.03Kg/animal, AC 1.16±0.01 and 1.16±0.01m, AG 1.2±0.01 and 1.2±0.01 m, LC 0.91±0.01 and 0.91±0.01m, PT 1.47±0.01 and 1.56±0.01m and GRD 2.76± 0.2 and 2.54±0.2 for T1 and T2 respectively. It’s enough to make a strategic supplementation in winter heifer rearing in forestry livestock systems. © 2015, Instituto Nacional de Investigaciones Agricolas (INIA). All rights reserved.


Candia O.A.,Mount Sinai School of Medicine | Gerometta R.M.,UNNE | Danias J.,SUNY Downstate Medical Center
Experimental Eye Research | Year: 2014

We have previously shown that tissue plasminogen activator (tPA) injected in the vitreous of sheep, reduced or prevented the elevation of the intraocular pressure (IOP) normally produced by the instillation of 1% prednisolone. We now report the effect of tPA when injected into the anterior chamber (AC) in amounts of 0.01, 0.001 and 0.0001μg diluted in a volume of 50μL. Lyophilized tPA, obtained as Actilyse® 50mg from Boehringer Ingelheim containing arginine was utilized. The Actilyse was diluted in balanced salt solution to obtain the desired amount of tPA in 50μL. An identical solution containing only arginine was prepared to inject into the contralateral eye as a control. Six sheep of the Corriedale breed were selected. At the beginning of the study all eyes received instillation of 1% prednisolone 3times/day for 10days to elevate their IOP from 10mmHg to about 23mmHg. Then, 0.0001μg was injected into one of the eyes and its effect was followed for up to 55:00h while the instillation of prednisolone continued in both eyes. The same protocol was implemented for the 0.001 and 0.01μg amounts after extended washout and IOP was over 22mmHg. The injection of 0.0001μg into the AC had no effect on an IOP of 23.0mmHg at 6:00 and 30:00h after injection. 0.001μg of tPA reduced IOP from 23.1 to 18.6mmHg at 6:00h but IOP recovered to 22.3mmHg at 30:00h. Injection of 0.01μg produced a marked and prolonged reduction of IOP. From a baseline of 23.0, IOP was reduced to 14.0, 14.7, 21.2, and 20.9mmHg at 5.0, 23.0, 27.0 and 45.5h, respectively. The 0.423μg of arginine, which is associated with 0.01μg tPA, was injected alone and had no effect. Recombinant human tPA injected in the AC is effective in reversing steroid-induced IOP elevation in sheep. The reduction of IOP elevation may be the result of an effect on extra-cellular matrix turnover in the TM. These findings suggest that tPA may by useful as a therapeutic agent in steroid-induced glaucomas. © 2014 Published by Elsevier Ltd.


Gerometta R.,UNNE | Gerometta R.,Mount Sinai School of Medicine | Kumar S.,SUNY Downstate Medical Center | Shah S.,SUNY Downstate Medical Center | And 3 more authors.
Investigative Ophthalmology and Visual Science | Year: 2013

Purpose. To investigate whether tissue plasminogen activator (tPA) can prevent and/or reverse steroid-induced IOP elevation in an ovine model. Methods. Three animal groups were subjected to bilateral steroid-induced IOP elevation using thrice daily topical ocular prednisolone administration. In the first group (N = 8), one eye each of two sheep was injected intravitreally with 100 μg, 200 μg, 500 μg, or 1 mg human recombinant tPA, while contralateral eyes received vehicle. In the second group (N = 2), one eye was injected intravitreally with tPA (100 μg), while contralateral eyes received vehicle containing L-arginine. In the third group (N = 4), each animal received intravitreal tPA in one eye concurrently with initiation of bilateral steroid administration. IOP was monitored for the duration of the experiment. Tissues from eyes of the third group were used to determine relative gene expression. Results. In the first and second groups, IOP decreased by 9.7 (±2.8) and 9.7 (±1.6) mm Hg, respectively, 24 hours after tPA administration. In the third group, tPA-treated eyes did not develop IOP elevation with ΔIOP of 11.8 (±1.3) mm Hg 8 days later. In all tPA-treated eyes, IOP remained low until the end of the study. mRNA levels in the trabecular meshwork were decreased for plasminogen activator tissue (PLAT), increased for matrix-metalloproteinase 1 (MMP-1), and stable for plasminogen activator inhibitor 1 (PAI-1), MMP-2, MMP-9, and MMP-13 in tPA-treated eyes compared with contralateral controls. PAI-1 mRNA levels in ciliary processes also remained similar. Conclusions. Recombinant human tPA is effective in both preventing and reversing steroid-induced IOP elevation in sheep. Tissue plasminogen activator may be useful as a therapeutic agent in steroid-induced glaucoma. © 2013 The Association for Research in Vision and Ophthalmology, Inc.


PubMed | Mount Sinai School of Medicine, SUNY Downstate Medical Center and UNNE
Type: | Journal: Experimental eye research | Year: 2014

We have previously shown that tissue plasminogen activator (tPA) injected in the vitreous of sheep, reduced or prevented the elevation of the intraocular pressure (IOP) normally produced by the instillation of 1% prednisolone. We now report the effect of tPA when injected into the anterior chamber (AC) in amounts of 0.01, 0.001 and 0.0001 g diluted in a volume of 50 L. Lyophilized tPA, obtained as Actilyse 50 mg from Boehringer Ingelheim containing arginine was utilized. The Actilyse was diluted in balanced salt solution to obtain the desired amount of tPA in 50 L. An identical solution containing only arginine was prepared to inject into the contralateral eye as a control. Six sheep of the Corriedale breed were selected. At the beginning of the study all eyes received instillation of 1% prednisolone 3 times/day for 10 days to elevate their IOP from 10 mm Hg to about 23 mm Hg. Then, 0.0001 g was injected into one of the eyes and its effect was followed for up to 55:00 h while the instillation of prednisolone continued in both eyes. The same protocol was implemented for the 0.001 and 0.01 g amounts after extended washout and IOP was over 22 mm Hg. The injection of 0.0001 g into the AC had no effect on an IOP of 23.0 mm Hg at 6:00 and 30:00 h after injection. 0.001 g of tPA reduced IOP from 23.1 to 18.6 mm Hg at 6:00 h but IOP recovered to 22.3 mm Hg at 30:00 h. Injection of 0.01 g produced a marked and prolonged reduction of IOP. From a baseline of 23.0, IOP was reduced to 14.0, 14.7, 21.2, and 20.9 mm Hg at 5.0, 23.0, 27.0 and 45.5 h, respectively. The 0.423 g of arginine, which is associated with 0.01 g tPA, was injected alone and had no effect. Recombinant human tPA injected in the AC is effective in reversing steroid-induced IOP elevation in sheep. The reduction of IOP elevation may be the result of an effect on extra-cellular matrix turnover in the TM. These findings suggest that tPA may by useful as a therapeutic agent in steroid-induced glaucomas.


Species diversity and abundance of Orthoptera communities were estimated in two grasslands of Eastern Humid Chaco from 2006 to 2007. The greatest species diversity was recorded in the grassland with intensive livestock grazing and predominance of native plant species, whereas the abundance values were higher in the grassland without grazing and characterized by introduced plant species. In total, 25 Orthoptera species were collected during the study. Acrididae (Caelifera) was the most abundant and diverse family recorded. Staurorhectus longicornis, Dichromorpha australis and Orphulella punctata were the most abundant species; the last two species being recorded during the entire sampling period. The greatest abundance was recorded in December 2006. Abundance did not show any correlation with climatic variables. © 2010 Museu de Ciències Naturals.


Blugerman G.,Clinica BandS | Schavelzon D.,Clinica BandS | D'Angelo S.,Clinica BandS | D'Angelo W.,Cirugia | Meirino A.,U.N.N.E.
Prensa Medica Argentina | Year: 2010

Introduction: The surgical dermoabration consists in the removal of the superficial dermis through out an abrasive process with sandpaper and/or diamonds drills that intends the attenuation of facial wrinkles, acne sequels and skin hiperpigmentation. The platelets rich plasma (PRP) is an autologous, nontoxic, nonalergenic product obtained from the patient's blood centrifugation, it contains 95% of platelet and it can be used topically over the post dermoabration wound and it accelerates the repair and healing of the wounds. Material and method: We treated 32 patients (12 male and 20 female) of 38 years average: 15 with acne sequels, 13 with facial wrinkles and 4 because of facial rejuvenation. We did the surgical dermoabration with local tumescent anesthesia infiltration using sandpaper and/or diamonds drill. The PRP is obtained from the patient's autologous venous blood centrifugation. Results: All the patients had satisfactory results with less scar formation and a faster reepitelization process Conclusion: In cosmetic and esthetic medicine there are many ways of uses the PRP. This paper shows our experience in the topical use of PRP in patients submitted to a surgical dermoabration. It's observed that when placing PRP in the wound the process of reepitelization of the wound is accelerated, allowing a better healing which is noticed as a shorter recovery time, avoiding infections and hematomas. It has not contraindications because is an autologous, nontoxic, nonalergenic product obtained by the patient blood centrifugation.


Rebak G.I.,FCV | Capellari A.,FCV | Ynsaurralde Rivolta E.A.,UNNE
Revista Veterinaria | Year: 2010

The buffalo population in the country is approximately 100,000 heads located in the northeastern provinces of Argentina, and there is a growing need of bulls selected for production systems. To improve the fertility of bulls is important to make use of reproductive characteristics of medium to high heritability and genetic correlations among themselves favorable for the production system, such as those related to growth. Within reproductive parameters, scrotal circumference (SC) is a feature to be included in breeding programs for beef bulls to be genetically linked, so favorable to the semen quality and reproductive precocity in females. Testicular development measured by scrotal circumference increases with growth and is correlated with body weight. The objectives of the study were to evaluate the buffalo bulls testicular development in post-weaning and correlate with bodyweight. The trial was conducted in Ita Ibaté; General Paz Department of the Province of Corrientes. The main activities include cattle and meat buffalo's production, with 1600 acres of of low-field for buffalos production. The animals used were bulls buffalos, Murrah crosses by Mediterranean. The animals come from an extensive system of service fall, autumn calving and weaning of spring. Feeding the animals were subjected to continuous grazing field was natural and the health plan used was recommended for cattle. Measurements in animals were: weight, height at the hips, gasping and measuring scrotal circumference at weaning and year and a half years. The scrotal circumference is positively correlated with body weight.


Molina S.P.,Instituto Nacional de Tecnologia Agropecuaria | Perez M.L.,UNNE | Rey H.Y.,Northeast National University | Mroginski L.A.,Northeast National University
Revista de la Facultad de Ciencias Agrarias | Year: 2013

Plants of two clones (CH 14 INTA and CH 318 INTA) of tea (Camellia sinensis (L.) O. Kuntze) were regenerated by in vitro culture of three types of explants disinfected by immersion in 70% ethanol (1 min) and 1.5% sodium hypochlorite (20 min). The best medium for shoot regeneration from uninodal segments, for both clones, as well as for axillary buds of CH 14 INTA clone was 1/2 MS + 1 mg/L BAP. While the best medium for axillary buds of CH 318 clone was 1/2 MS + 1 mg/L BAP + 1 mg/L AG3 For meristems culture, the best medium, for both clones was 1/2 MS + 1 mg/L KIN + 1 mg/L AG3. Rooting of regenerated shoots were achieved by culture them on 1/4 MS + 6 mg/L IBA.


PubMed | UNNE
Type: Comparative Study | Journal: Investigative ophthalmology & visual science | Year: 2013

To investigate whether tissue plasminogen activator (tPA) can prevent and/or reverse steroid-induced IOP elevation in an ovine model.Three animal groups were subjected to bilateral steroid-induced IOP elevation using thrice daily topical ocular prednisolone administration. In the first group (N = 8), one eye each of two sheep was injected intravitreally with 100 g, 200 g, 500 g, or 1 mg human recombinant tPA, while contralateral eyes received vehicle. In the second group (N = 2), one eye was injected intravitreally with tPA (100 g), while contralateral eyes received vehicle containing L-arginine. In the third group (N = 4), each animal received intravitreal tPA in one eye concurrently with initiation of bilateral steroid administration. IOP was monitored for the duration of the experiment. Tissues from eyes of the third group were used to determine relative gene expression.In the first and second groups, IOP decreased by 9.7 (2.8) and 9.7 (1.6) mm Hg, respectively, 24 hours after tPA administration. In the third group, tPA-treated eyes did not develop IOP elevation with IOP of 11.8 (1.3) mm Hg 8 days later. In all tPA-treated eyes, IOP remained low until the end of the study. mRNA levels in the trabecular meshwork were decreased for plasminogen activator tissue (PLAT), increased for matrix-metalloproteinase 1 (MMP-1), and stable for plasminogen activator inhibitor 1 (PAI-1), MMP-2, MMP-9, and MMP-13 in tPA-treated eyes compared with contralateral controls. PAI-1 mRNA levels in ciliary processes also remained similar.Recombinant human tPA is effective in both preventing and reversing steroid-induced IOP elevation in sheep. Tissue plasminogen activator may be useful as a therapeutic agent in steroid-induced glaucoma.


PubMed | UNNE
Type: Journal Article | Journal: Investigative ophthalmology & visual science | Year: 2010

To investigate whether intracameral injection of the adenovirus vector AdhGRE.MMP1 would reduce or prevent elevated intraocular pressure (IOP) induced by corticosteroids in living animals.Glucocorticoid-inducible adenovirus vectors carrying wild-type or mutant forms of human metalloproteinase 1 (MMP1 and mutMMP1) cDNAs were generated. An adenovirus carrying no gene (Ad5.CMV.Null) was used as an additional control. Sheep were injected intracamerally with 30 microL of each vector, either previously or after the induction of increased IOP with topical prednisolone or sub-Tenon triamcinolone under various protocols. IOP was measured with a Perkins tonometer. Inflammation was monitored by visual inspection.In eyes in which IOP was already elevated to 24 to 30 mm Hg, injection of AdhGRE.MMP1 reduced IOP by 70% in 24 hours and to 10 to 13 mm Hg in 48 hours. In eyes with normal IOP (9-11 mm Hg), preinjection of the virus protected against the increase in IOP normally produced by the corticosteroid. IOP remained at a level of approximately 12 mm Hg for 5 days despite the continuous application of the corticosteroid. Injections of the control viruses had no hypotensive effects. There were no signs of ocular inflammation or discomfort to the animals.A single dose of a gene therapy vector carrying an inducible metalloproteinase human gene can both protect against the IOP increase produced by corticosteroid instillation in the sheep model and quickly reverse the IOP increase previously elicited by the corticosteroid. These results are a first step toward a treatment of steroid-glaucoma with inducible overexpression of extracellular matrix modulator genes.

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