Aya, Japan
Aya, Japan

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Matsuura Y.,University of Miyazaki | Matsuura Y.,Miyazaki Prefectural Food R and nter | Kaneko M.,University of Miyazaki | Hirabaru H.,Unkai Shuzo Co. | And 6 more authors.
Nippon Shokuhin Kagaku Kogaku Kaishi | Year: 2013

We evaluated the effects of dried blueberry leaves (BL) and its hot-water extract (BLEx) on blood pressure of spontaneously hypertensive rats (SHR). A continuous 3-week administration of BL (3 %) and BLEx (1.5 %) reduced systolic blood pressure compared to the control group from the 2-week feeding point. Additionally, we fractionated BLEx and investigated the compound responsible for the enhanced blood pressure-lowering effect. BLEx were fractionated into 5-fractions using Sephadex LH-20 and Diaion HP20SS chromatography, and assayed for angiotensin-I-converting enzyme (ACE) inhibitory activity in vitro. The fraction composed of proanthocyanidins showed the most potent inhibitory activity (IC50, 0.004 mg/mL) and reduced systolic blood pressure of SHR. These results suggest that BL and BLEx have anti-hypertensive effects and one of the active components has been identified as a proanthocyanidin.


Yuji K.,Miyazaki Prefectural Industrial Support Foundation | Yuji K.,University of Miyazaki | Sakaida H.,Unkai Shuzo Co. | Kai T.,Unkai Shuzo Co. | And 5 more authors.
Journal of Oleo Science | Year: 2013

The serum and liver lipid-lowering effects of dietary freeze-dried blueberry leaf powder (BL) and its hydrothermal extract (BLHE) were examined in rats fed diets with or without cholesterol supplementation. Administration of 1% and 3% BL had no adverse effects on food intake or growth; however, relative liver weights were reduced in rats fed diets with and without dietary cholesterol. In the absence of dietary cholesterol, a dose-dependent reduction was evident. The effects of dietary BL on the concentration of serum lipids were marginal; however, the effects on liver triacylglycerol (TG) and cholesterol levels were apparently dose-dependent when the animals were fed diets free of cholesterol. Further, BL significantly attenuated dietary cholesterol-dependent accumulation of hepatic cholesterol, but not of TG. Hydrothermal treatment studies suggested that the active component of BL in terms of its liver lipid-lowering activity is relatively stable at high temperatures. Histopathological analysis of hepatic tissues revealed that BL administration suppresses fatty infiltrations induced by an AIN 76-based high-sucrose diet. The results of this study suggest that some of the active components of BL extracts, which are incorporated into the liver, prevent fatty liver in rats. These results provide further support for the investigation of dietary BL and derivatives thereof as functional human foods. © 2013 by Japan Oil Chemists' Society.


Inoue N.,Saga University | Inoue N.,Tohoku University | Nagao K.,Saga University | Nomura S.,Saga University | And 7 more authors.
Bioscience, Biotechnology and Biochemistry | Year: 2011

The effects of a hot water extract and fractional extracts from rabbiteye blueberry (Vaccinium ashei reade) leaves (BBL) on lipid metabolism were studied in obese Otsuka Long-Evans Tokushima Fatty (OLETF) rats. Feeding the hot water extract and fractional extracts from BBL alleviated hepatic triglyceride accumulation in the rats. Additionally, feeding with the flavonol glycoside (FG) and proanthocyanidin (PA) fractions lowered serum cholesterol levels in the obese rats. The results from measurements of the hepatic enzyme activity indicate that the hypolipidemic effects of the hot water extract and the PA fraction might be attributable to enhanced lipolysis in the liver. The reduced serum levels of C-reactive protein, an inflammatory cytokine, by the chlorogenic acid + rutin fraction and FG fraction might be associated with alleviating the metabolic abnormalities in obese rats. These results indicate that the BBL extracts, and especially FG and PA, exerted hypolipidemic effects on obese OLETF rats and suggest that an infusion of BBL can be useful as a dietary hypolipidemic component.


Kai T.,Unkai Shuzo Co. | Tanimura T.,Unkai Shuzo Co. | Nozaki N.,Unkai Shuzo Co. | Suiko M.,University of Miyazaki | Ogawa K.,University of Miyazaki
Seibutsu-kogaku Kaishi | Year: 2010

Bioconversion of soft cellulosic resources such as rice straw, and napiergrass into sugar and ethanol was examined using the genetically modified cellulase, Accellerase 1000 preparation and compared with the use of other cellulase preparations. Treatment of soft cellulosic resources with 0.25 M sodium hydroxide solution at 100°C for 1 h was considered to be a practical method for delignification. A sugar solution containing 15-20% reducing sugar at 15-20% substrates (w/v) was produced by saccharification of delignified rice straw and napiergrass using Accellerase 1000 preparation at 45°C for 72 h. The reducing sugars after saccharification were glucose and xylose at a ratio of about 7 : 3. A concentrated reducing sugar solution (24.3%) derived from delignified rice straw which included Henneberg's inorganic salts was incubated at pH 5.0 and 28°C for 3 d after inoculation with Saccharomyces cerevisiae TV2. The ethanol concentration in the fermented solution was 6.3%, but xylose was not utilized by the yeast. In conclusion, sugar and ethanol production from soft cellulosic resources may be realized in the near future.


Nozaki N.,Unkai Shuzo Co. | Nakahara N.,Unkai Shuzo Co. | Yamamoto H.,Miyazaki Prefecture Foods Development Center | Sakakibara Y.,University of Miyazaki | And 3 more authors.
Seibutsu-kogaku Kaishi | Year: 2011

The Potential of 16 Strains of Black Filamentous Fungi Isolated from Chi-chuwas Investigated for Use as Koji in Shochu Making. of the 16 Black Filamentous Fungi Strains Cultiv. on Rice Solid Culture Koji, 5 Strains-KNO4 and KNO9 (Belonging to the Aspergillus Niger Tiegh. Sp.) and KNO16, KNO17, and KNO18 (Belonging to the Aspergillus Sp.) -Exhibited High Capabilities of Citric Acid Prod. and High Enz. Activ. Compared to Aspergillus Awamori RIB 2602. on Further Screening, KNO16 and KNO18 Were Selected Based on Their Ext. Characteristics, Prod. of Stable Conidia, and High Acid Carboxypeptidase Activ. Compared to A. Awamori RIB 2602. Further, Unlike A. Awamori, These Strains Did Not Produce 2-Methylisoborneol and Geosmin, Which is Responsible for A High Fragrant Flavor. Addition of Artif. Ash to Rice and Barley Media Promoted 80% Germination, and the Strains Produced 10 9g-1 of conidia in the koji. Importantly, the 2 selected strains did not produce anatoxin or ochratoxin in the koji solid culture. The acid carboxypeptidase activity of KNO16 and KNO18 was >2.6 fold that of A. awamori RIB 2602. The 2 selected strains were used for alcoholic fermentation tests of laboratory scale under optimal temperature conditions for citric acid and enzyme productions in the koji. KNO16 and KNO18 had alcoholic fermentation rates that corresponded to that of commercial strain A. awamori. Further, the selected strains showed highly productivity of isoamyl alcohol with commercial strain A. awamori. From these results, it was concluded that the novel strains, KNO16 and KNO18, are very useful for shochu making.


Matsuo Y.,Nagasaki University | Fujita Y.,Nagasaki University | Ohnishi S.,Nagasaki University | Tanaka T.,Nagasaki University | And 5 more authors.
Food Chemistry | Year: 2010

Chemical constituents of the leaves of rabbiteye blueberry (Vaccinium ashei READE) were investigated in detail. The major phenolic components were caffeoyl quinic acids, flavonol glycosides, flavan-3-ols and proanthocyanidins. Catechins and proanthocyanidins having additional phenylpropanoid units, such as cinchonains, kandelins and mururins, characterised the polyphenols of this plant. Among them, vaccinin A, an isomer of mururin A, was found to be a new compound, and the structure was characterised by spectroscopic methods. The most abundant polyphenols (11.3% of freeze-dried leaves) were oligomeric proanthocyanidins. Thiol degradation with mercaptoethanol indicated that the polymer was constituted of (+)-catechin and (-)-epicatechin as the terminal units and (-)-epicatechin, procyanidin A-2, and cinchonains Ia and Ib as the extension units. Mass spectral analysis suggested the presence of at least dodecamers with A-type linkages and phenylpropanoid moieties. © 2010 Elsevier Ltd. All rights reserved.


Patent
Miyazaki Prefectural Industrial Support Foundation, Minami Nippon Dairy Co Op Co., Unkai Shuzo Co. and Miyazaki Prefecture | Date: 2011-08-05

This invention provides an agent for inhibiting production of hepatitis C virus with notable anti-HCV activity and without side-effects. The agent comprises a proanthocyanidin polymer composition illustrated in the following the general formula (1),

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