University Telematica Pegaso

Napoli, Italy

University Telematica Pegaso

Napoli, Italy

Time filter

Source Type

Borbone N.,University of Naples Federico II | Elce A.,University Telematica Pegaso | Amato J.,University of Naples Federico II | D'Errico S.,University of Naples Federico II | And 4 more authors.
MedChemComm | Year: 2014

Recently, microRNAs (miRNAs) were identified as being able to inhibit the expression of the Cystic Fibrosis Transmembrane Regulator (CFTR) disease-gene of Cystic Fibrosis (CF) and CFTR-Related Disorders (CFTR-RD). This study shows the use of peptide nucleic acids (PNAs) as inhibitors of miR-509-3p, one of the CFTR regulating miRNAs, by reverting the expression of the luciferase gene containing the 3′UTR of CFTR gene. © 2014 The Royal Society of Chemistry.


PubMed | Centro Regionale Toscano per la Fibrosi Cistica, Unita di Fibrosi Cistica, Laboratorio Genetica Medica, University Telematica Pegaso and 9 more.
Type: | Journal: Journal of medical genetics | Year: 2016

The effect of complex alleles in cystic fibrosis (CF) is poorly defined for the lack of functional studies.To describe the genotype-phenotype correlation and the results of either in vitro and ex vivo studies performed on nasal epithelial cells (NEC) in a cohort of patients with CF carrying cystic fibrosis transmembrane conductance regulator (CFTR) complex alleles.We studied 70 homozygous, compound heterozygous or heterozygous for CFTR mutations: p.[Arg74Trp;Val201Met;Asp1270Asn], n=8; p.[Ile148Thr;Ile1023_Val1024del], n=5; p.[Arg117Leu;Leu997Phe], n=6; c.[1210-34TG[12];1210-12T[5];2930C>T], n=3; p.[Arg74Trp;Asp1270Asn], n=4; p.Asp1270Asn, n=2; p.Ile148Thr, n=6; p.Leu997Phe, n=36. In 39 patients, we analysed the CFTR gating activity on NEC in comparison with patients with CF (n=8) and carriers (n=4). Finally, we analysed in vitro the p.[Arg74Trp;Val201Met;Asp1270Asn] complex allele.The p.[Ile148Thr;Ile1023_Val1024del] caused severe CF in five compound heterozygous with a class I-II mutation. Their CFTR activity on NEC was comparable with patients with two class I-II mutations (mean 7.3% vs 6.9%). The p.[Arg74Trp;Asp1270Asn] and the p.Asp1270Asn have scarce functional effects, while p.[Arg74Trp;Val201Met;Asp1270Asn] caused mild CF in four of five subjects carrying a class I-II mutation in trans, or CFTR-related disorders (CFTR-RD) in three having in trans a class IV-V mutation. The p.[Arg74Trp;Val201Met;Asp1270Asn] causes significantly (p<0.001) higher CFTR activity compared with compound heterozygous for class I-II mutations. Furthermore, five of six compounds heterozygous with the p.[Arg117Leu;Leu997Phe] had mild CF, whereas the p.Leu997Phe, in trans with a class I-II CFTR mutation, caused CFTR-RD or a healthy status (CFTR activity: 21.3-36.9%). Finally, compounds heterozygous for the c.[1210-34TG[12];1210-12T[5];2930C>T] and a class I-II mutation had mild CF or CFTR-RD (gating activity: 18.5-19.0%).The effect of complex alleles partially depends on the mutation in trans. Although larger studies are necessary, the CFTR activity on NEC is a rapid contributory tool to classify patients with CFTR dysfunction.


Persia F.,University of Naples Federico II | D'Auria D.,University of Naples Federico II | Sperli G.,University of Naples Federico II | Tufano A.,University Telematica Pegaso
Communications in Computer and Information Science | Year: 2015

Security has been raised at major public buildings in the most famous and crowded cities all over the world following the terrorist attacks of the last years, the latest one at the Bardo museum in the centre of Tunis. For that reason, video surveillance systems have become more and more essential for detecting and hopefully even prevent dangerous events in public areas. In this paper, we present a prototype for anomaly detection in video surveillance context. The whole process is described, starting from the video frames captured by sensors/cameras till at the end some well-known reasoning algorithms for finding potentially dangerous activities are applied. The conducted experiments confirm the efficiency and the effectiveness achieved by our prototype. © Springer International Publishing Switzerland 2015.


Santolo A.S.D.,University Telematica Pegaso | Forte G.,University of Naples Federico II | De Falco M.,University of Naples Federico II | Santo A.,University of Naples Federico II
Procedia Engineering | Year: 2016

The city of Naples and its neighboring is an example of urban area affected by frequent anthropogenic sinkholes. They occur where the mining of tuff at shallow depth left a wide and complex network of cavities. The collapse is usually triggered by the soaking of the overlying pyroclastic soils forwarded by the presence of leakage from aged aqueducts and sewerages. This note reports the first results of a multidisciplinary research activity aimed at enhance the knowledge of the triggering factors of these phenomena in urban contexts. The study focused on an area characterized by the presence of cavities dug in tuff, starting from the research and collection of their location and that of past collapse events. In particular, the paper presents the results of sinkhole occurrence assessment at both local and metropolitan scale. In the first case, in order to define the most likely triggering mechanisms a case study among the recent sinkholes was investigated. A detailed field survey of the phenomena permitted to define the stratigraphical and geometrical setting of the pre-existing cavity and collect soil and rock samples for the geotechnical characterization. The attained results permitted to identify the most relevant parameters that influence the susceptibility assessment in a study area at metropolitan scale. This study represents contribute to the definition of a procedure to study anthropogenic sinkhole in intensely urbanized areas and it represents a valuable support for future planning strategies of risk mitigation. © 2016 The Authors.


Nigro E.,CEINGE Biotecnologie Avanzate Scarl | Sangiorgio D.,University Telematica Pegaso | Scudiero O.,CEINGE Biotecnologie Avanzate Scarl | Scudiero O.,University of Naples Federico II | And 5 more authors.
Cytokine | Year: 2016

Metabolic Syndrome prevalence has reaching epidemic proportions worldwide. Adiponectin (Acrp30), and in particular its High Molecular Weight (HMW) oligomers, contributes to enhance insulin sensitivity and to reduce inflammation levels. Physical exercise improves body's biochemical balance and metabolism resulting effective in prevention of metabolic diseases. Whether improvement of metabolic features mediated by physical exercise is associated with changes in Acrp30 serum composition is not yet clarified. In the present study, we investigated total Acrp30 expression, its oligomeric status and genetic variants in adiponectin gene (ACDC) in twenty-two professional Water Polo (WP) Players and 40 age- and sex-matched controls. Anthropometric, metabolic parameters and total Acrp30 were assessed; Acrp30 oligomeric profile was characterized by Western blot as well as by FPLC analysis. ACDC gene was analyzed by direct-sequencing analysis. Significant elevated body mass index, aspartate aminotransferase and lactate dehydrogenase levels and, conversely, significantly lower concentrations of total and cholesterol low density lipoprotein were present in WP players. No significant difference was found in total Acrp30 and/or HMW oligomers. Interestingly, in WP players, a direct relationship between total Acrp30 and monocytes as well as an inverse relationship between total Acrp30 and AST levels were found. ACDC screening revealed previously described SNPs. In conclusion, our study confirms the long-term beneficial effects of high physical training on metabolism and suggests that they are not associated with Acrp30 and/or HMW oligomers changes. Moreover, the correlation of Acrp30 with monocytes in WP athletes could represent a mechanism by which Acrp30 participates in exercise-induced anti-inflammatory functions and/or cardiovascular health. © 2016.


PubMed | University of Naples Federico II, Pediatria Sistematica, University Telematica Pegaso and Bambino Gesu Childrens Hospital
Type: | Journal: Molecular medicine (Cambridge, Mass.) | Year: 2016

Genetic features of Chronic Pancreatitis (CP) have been extensively investigated mainly testing genes associated to the trypsinogen activation pathway. However, different molecular pathways involving other genes may be implicated in CP pathogenesis.80 patients with Idiopathic CP were investigated using Next Generation Sequencing approach with a panel of 70 genes related to six different pancreatic pathways: premature activation of trypsinogen; modifier genes of Cystic Fibrosis phenotype; pancreatic secretion and ion homeostasis; Calcium signalling and zymogen granules exocytosis; autophagy; autoimmune pancreatitis related genes.We detected mutations in 34 out of 70 genes examined; 64/80 patients (80.0%) were positive for mutations in one or more genes, 16/80 patients (20.0%) had no mutations. Mutations in Our data, although to be extended with functional analysis of novel mutations, suggest a high rate of genetic heterogeneity in chronic pancreatitis and that trans-heterozygosity may predispose to the idiopathic CP phenotype.


PubMed | University of Naples Federico II and University Telematica Pegaso
Type: Journal Article | Journal: Clinica chimica acta; international journal of clinical chemistry | Year: 2015

Mannose binding lectin (MBL) is a protein of innate immunity that activates the complement and promotes opsonophagocytosis. The deficiency of MBL due to several common gene polymorphisms significantly enhances the risk of severe infections, particularly in the neonatal age and in childhood. On the contrary, the role of the protein in carcinogenesis and atherogenesis is still debated: MBL has a relevant role against neoplastic cells, but some studies described a protective effect of low levels of MBL toward breast cancer and a longer survival of lung cancer patients with a reduced MBL activity. Similarly, some studies concluded on the protective role of low levels of MBL toward cardiovascular diseases while other focused on a higher risk of myocardial infarction in subjects with a deficient activity of the protein. More recently, a role of MBL in the clearance of senescent cells emerged, and a study in two large cohorts of centenarians demonstrated that a high biological activity of the protein enhances the risk of autoimmune diseases. This body of data strongly suggests that the optimal levels of MBL activity depend on the age and on the environmental context of each subject.


PubMed | CEINGE Biotecnologie Avanzate Scarl, University of Molise, The Second University of Naples, University of Naples Federico II and University Telematica Pegaso
Type: | Journal: Cytokine | Year: 2016

Metabolic Syndrome prevalence has reaching epidemic proportions worldwide. Adiponectin (Acrp30), and in particular its High Molecular Weight (HMW) oligomers, contributes to enhance insulin sensitivity and to reduce inflammation levels. Physical exercise improves bodys biochemical balance and metabolism resulting effective in prevention of metabolic diseases. Whether improvement of metabolic features mediated by physical exercise is associated with changes in Acrp30 serum composition is not yet clarified. In the present study, we investigated total Acrp30 expression, its oligomeric status and genetic variants in adiponectin gene (ACDC) in twenty-two professional Water Polo (WP) Players and 40 age- and sex-matched controls. Anthropometric, metabolic parameters and total Acrp30 were assessed; Acrp30 oligomeric profile was characterized by Western blot as well as by FPLC analysis. ACDC gene was analyzed by direct-sequencing analysis. Significant elevated body mass index, aspartate aminotransferase and lactate dehydrogenase levels and, conversely, significantly lower concentrations of total and cholesterol low density lipoprotein were present in WP players. No significant difference was found in total Acrp30 and/or HMW oligomers. Interestingly, in WP players, a direct relationship between total Acrp30 and monocytes as well as an inverse relationship between total Acrp30 and AST levels were found. ACDC screening revealed previously described SNPs. In conclusion, our study confirms the long-term beneficial effects of high physical training on metabolism and suggests that they are not associated with Acrp30 and/or HMW oligomers changes. Moreover, the correlation of Acrp30 with monocytes in WP athletes could represent a mechanism by which Acrp30 participates in exercise-induced anti-inflammatory functions and/or cardiovascular health.


Fabbrocino F.,University Telematica Pegaso
Civil-Comp Proceedings | Year: 2014

The fundamental period of vibration plays a primary role in the assessment of the seismic demand on structures. A reliable evaluation of dynamic and seismic response of structures requires an accurate estimation of their modal properties. This is a relevant aspect for both the design of new structures and the assessment and strengthening of existing ones. In the case of historical structures the accuracy of numerical analysis is critical because interventions have to be carefully calibrated in order to limit their invasiveness. Structural modal properties can be obtained from an eigenvalue analysis of its mechanical model, or either from empirical correlations as a function of geometric parameters (usually the height), whenever prescribed regularity conditions are fulfilled. In this paper, starting from available experimental results from output-only modal identification tests on ancient masonry towers in the Molise region, of Southern Italy, numerical analyses were performed with the aim of investigating the relevant factors influencing the structural dynamic response of the towers described in this paper. A numerical procedure able to solve an eigenvalue problem for a Timoshenko beam has been developed and used to carry out extensive sensitivity analyses. The final objective of the analyses described in this paper, was the quantitative assessment of the influence of the relevant geometric and mechanical parameters on the dynamic response of historical masonry towers. The aim was the development of a formulation for the prediction of the fundamental period as a function of the height, based on the analogy with similar correlations reported in the literature and in design codes for selected structural typologies. © Civil-Comp Press, 2014.


Iodice P.,University Telematica Pegaso | Massarotti N.,Parthenope University of Naples | Mauro A.,Parthenope University of Naples
Energies | Year: 2016

The interest of calculating the effects of thermal bridges in buildings energy consumption is growing, due to recent energy saving regulations applied in different countries. The widespread use of insulating materials to reduce energy requirements of buildings, often employed for intermediate insulation of the building envelope, makes thermal bridges a crucial point in the energy analysis of building envelopes. Furthermore, heat losses through thermal bridges often lead to building pathologies due to moisture condensation. Therefore, thermal bridges need to be correctly characterized in the building design stage in order to reduce heat losses and avoid materials degradation. The authors numerically simulate, by using finite elements, the steady-state and dynamic three-dimensional (3D) heat and vapor transport in inhomogeneous thermal bridges and building envelopes. The aim of the present work is to show the importance of taking into account the presence of inhomogeneities (i.e., metal stud) in building materials for the calculation of actual heat losses and water condensation in 3D thermal bridges. The obtained heat transfer results are verified against the reference data of the technical standard UNI EN ISO 10211. The proposed microscopic approach is essential to calculate the actual heat losses of three-dimensional thermal bridges and building envelopes and to overcome condensation problems. © 2016 by the authors.

Loading University Telematica Pegaso collaborators
Loading University Telematica Pegaso collaborators