University Research Farm

India

University Research Farm

India
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Karunakaran M.,Tamil Nadu Veterinary and Animal Sciences University | Karunakaran M.,ICAR Research Complex for Goa | Devanathan T.G.,Tamil Nadu Veterinary and Animal Sciences University | Jawahar T.P.,Tamil Nadu Veterinary and Animal Sciences University | And 9 more authors.
Indian Journal of Animal Sciences | Year: 2012

Proteins present in the seminal plasma and sperm influence sperm function and fertilization. The present study was carried out to characterize the electrophoretic profile of bull sperm membrane proteins and to correlate with bull fertility. Semen samples were collected from 20 breeding bulls (10 Jersey and 10 Jersey crossbred) and the sperm membrane proteins were extracted by Triton X. Heparin binding proteins were eluted with heparin column, and the molecular weight of the proteins was assessed by discontinuous sodium dodecyl sulphate polyacrylamide gel elecrophoresis (SDSPAGE). To assess the field fertility of the bulls, 50 frozen semen straws/bull were used for insemination. Results indicated that only 50 % of the bulls screened had 28-30 kDa heparin binding proteins in their sperm and these bulls had 12 % higher conception than the bulls lack the protein. So, it can be concluded that the bulls positive for of 28-30 kDa heparin binding proteins in sperm membrane protein had higher chance of fertility and screening for its presence can be used as a tool in addition to routine criteria for selecting bulls.


Karunakaran M.,Tamil Nadu Veterinary and Animal Sciences University | Devanathan T.G.,Tamil Nadu Veterinary and Animal Sciences University | Jawahar T.P.,Tamil Nadu Veterinary and Animal Sciences University | Jawahar T.P.,University Research Farm | And 5 more authors.
Indian Journal of Animal Sciences | Year: 2013

The addition of heparin binding proteins to frozen thawed bull semen samples were able to preserve the vital in vitro sperm characters essential for successful fertilization such as sperm motility, functional membrane integrity and mitochondrial membrane potential The proteins were also able to reduce the oxidative stress to the sperm cells during incubation as indicated by less production of lipid peroxide compounds.


Karunakaran M.,Tamil Nadu Veterinary and Animal Sciences University | Karunakaran M.,ICAR Research Complex for Goa | Devanathan T.G.,Tamil Nadu Veterinary and Animal Sciences University | Devanathan T.G.,Madras Veterinary College | And 12 more authors.
Indian Journal of Animal Sciences | Year: 2012

Level of lipid peroxides in frozen thawed bull semen samples was studied in frozen semen straws procured from 22 bulls maintained at bull stations. The semen samples were thawed at 37°C in a water-bath and the level of lipid peroxide was measured by estimating malondialdehyde (MDA) concentration in the semen samples at 0, 60, 120 and 180 min of incubation by thiobarbituric acid assay. 28-30 kDa heparin binding protein (HBP) of sperm membrane @ 25 μg was added to the frozen thawed semen samples and the level of MDA was estimated to check whether addition of HBP of sperm membrane would control the production of lipid peroxides. MDA level (ìmol/ml) increased significantly during different periods of incubation-60 min, 120 min and 180 min post thaw in both control and treatment groups. MDA level was significantly lower (P < 0.01) in HBP treated semen sample than control semen at each period of incubation 1.95 vs 2.85; 2.40 vs 3.20; 2.99 vs 4.31 at 60, 120 and 180 min, respectively post thaw. MDA level in H2O2 treated samples increased significantly from 0 min level 1.67±0.1 to 1.85±0.1 at 10 min, 3.05±0.11 at 30 min and to 3.28±0.11 at 60 min incubation. The MDA level in H2O2 treated group (3.28±0.11) was significantly higher than the control (2.85±0.12) and HBP treated group (1.95±0.11) at 60 min incubation period. It was concluded that excessive H2O2 causes significant increases in lipid peroxides level and 28 - 30 kDa HBP of the sperm membrane could help in controlling the oxidative stress of the sperm cells.

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