Muravyov A.V.,Ushinsky University of Yaroslavl |
Koshelev V.B.,Moscow State University |
Fadyukova O.E.,Moscow State University |
Tikhomirova I.A.,Ushinsky University of Yaroslavl |
And 2 more authors.
Erythrocyte deformability is an important determinant of red blood cell (RBC) transport capacity and depends on at least three key factors: shape, internal viscosity and membrane mechanical properties. These properties can be modified by phosphorylation of key membrane proteins. At the same time, the whole signaling pathway that is connected with the change of RBC microrheology was not investigated properly. The aim of this study was to investigate the role of adenylyl cyclase (AC) system in RBC microrheology changes. Incubation of RBC with isoproterenol led to an increase of the RBC deformability (RBCD) by 30% (p < 0.01). When an AC stimulator forskolin (10 μM) was added to the RBC suspension, RBCD increased (p < 0.05). Somewhat more significant deformability rise appeared after the RBC incubation with dB-AMP (by 24%; p < 0.01). RBC aggregation (RBCA) decreased significantly under these conditions (p <0.01). All drugs having phosphodiesterase (PDE) activity similarly increased RBCD. Some more changes in RBCD were found after the incubation of RBC with isobutyl methybcantine (IBMX). Incubation was accompanied only by 22% rise of RBCD. The drugs inhibiting the PDE activity reduced RBCA. The most significant RBCA reduction was produced by IBMX and the PDE[ inhibitor vinpocetine. On the whole, the data suggest that the changes in RBC aggregation anu deformation were related with activation of the adenylyl cy-clase-cAMP intracellular signaling pathways. Source