Nardi G.M.,Federal University of Santa Catarina |
Nardi G.M.,University of West Santa Catarina |
Scheschowitsch K.,Federal University of Santa Catarina |
Ammar D.,Federal University of Santa Catarina |
And 3 more authors.
Critical Care Medicine | Year: 2014
OBJECTIVE: Vascular dysfunction plays a central role in sepsis, and it is characterized by hypotension and hyporesponsiveness to vasoconstrictors. Nitric oxide is regarded as a central element of sepsis vascular dysfunction. The high amounts of nitric oxide produced during sepsis are mainly derived from the inducible isoform of nitric oxide synthase 2. We have previously shown that nitric oxide synthase 2 levels decrease in later stages of sepsis, whereas levels and activity of soluble guanylate cyclase increase. Therefore, we studied the putative role of other relevant nitric oxide sources, namely, the neuronal (nitric oxide synthase 1) isoform, in sepsis and its relationship with soluble guanylate cyclase. We also studied the consequences of nitric oxide synthase 1 blockade in the hyporesponsiveness to vasoconstrictors. DESIGN: Randomized controlled prospective experimental study. SETTING: Academic research laboratory. SUBJECTS: Female Wistar rats submitted to cecal ligation and puncture method. INTERVENTIONS: 1) Six, 12, and 24 hours after cecal ligation and puncture, vascular reactivity to phenylephrine (3 and 30 nmol/kg) before and after 7-nitroindazole (45 μmol/kg, s.c.) or aminoguanidine (30 μmol/kg, s.c.) administration was evaluated. 2) Protein levels and interaction between nitric oxide synthase 1 and soluble guanylate cyclase were determined. 3) Six, 12, and 24 hours after cecal ligation and puncture, thoracic aorta segments were stimulated with phenylephrine in the presence or absence of 7-nitroindazole and cyclic guanosine monophosphate accumulation was determined. 4) After 24 hours of cecal ligation and puncture, norepinephrine was infused (10 μg/kg/min) in the presence or absence of 7-nitroindazole or S-methyl-L- thiocitrulline (1 μmol/kg, IV) and mean arterial pressure was registered. MEASUREMENTS AND MAIN RESULTS: 1) Both nitric oxide synthase 1 and soluble guanylate cyclase are expressed in higher levels in vascular tissues during sepsis; 2) both proteins physically interact and nitric oxide synthase 1 blockade inhibits cyclic guanosine monophosphate production; 3) pharmacological blockade of nitric oxide synthase 1 using 7-nitroindazole or S-methyl- L-thiocitrulline reverts the hyporesponsiveness to phenylephrine and increases the vasoconstrictor effect of norepinephrine and phenylephrine. CONCLUSIONS: Sepsis induces increased expression and physical association of nitric oxide synthase 1/soluble guanylate cyclase and a higher production of cyclic guanosine monophosphate that together may help explain sepsis-induced vascular dysfunction. In addition, selective inhibition of nitric oxide synthase 1 restores the responsiveness to vasoconstrictors. Therefore, inhibition of nitric oxide synthase 1 (and possibly soluble guanylate cyclase) may represent a valuable alternative to restore the effectiveness of vasopressor agents during late sepsis. © 2014 by the Society of Critical Care Medicine and Lippincott Williams & Wilkins.
Scaratti D.,University of West Santa Catarina |
Calvo M.C.M.,Federal University of Santa Catarina
Revista de Saude Publica | Year: 2012
OBJECTIVE: To develop a composite indicator to evaluate the quality of municipal management of primary health care. METHODS: The evaluation model focuses on aspects of health system management. Fifty-fi ve performance indicators were used and classifi ed according to the criteria of relevance, effectiveness, effi cacy and effi ciency. The measures were aggregated through an additive data envelopment analysis model for measures of value, merit and quality. Data was utilized from 36 municipalities in Santa Catarina State (Southern Brazil), with populations between 10 thousand and 50 thousand residents in 2006. RESULTS: The results are presented as monotonic measures over the interval [0, 1] (score = 1: effi cient; other values: ineffi cient). Five municipalities had a score of 1 in the quality of management for actions promoting access, while eight municipalities received a score of 1 in the quality of management of actions for service provision; the other municipalities were classifi ed as ineffi cient (score < 1) for both dimensions. CONCLUSIONS: The quality of municipal management in primary health care can be evaluated with a composite indicator, constructed through linear programming techniques, which simultaneously considers the criteria of relevance, effectiveness, effi cacy and effi ciency and expresses them as measures of value, merit and quality.
Locatelli C.,University of West Santa Catarina |
Locatelli C.,University Do Alto Vale Do Rio Do Peixe |
Filippin-Monteiro F.B.,Federal University of Santa Catarina |
Creczynski-Pasa T.B.,Federal University of Santa Catarina
European Journal of Medicinal Chemistry | Year: 2013
The current review presents the antitumoral properties of gallic acid and its ester derivatives. Numerous studies have indicated that the alkyl esters are more effective against tumor cell lines than gallic acid, and that this activity is related to their hydrophobic moiety. All related studies have shown that the antitumor activity is interconnected to the induction of apoptosis by different mechanisms and it depends on the cell type. The results presented in this review may help to emphasize that these compounds could be promising as a new alternative for the treatment of cancer, either alone or in combination with other antitumor drugs to potentiate their effects.
Hellmann F.,University of Southern Santa Catarina |
Hellmann F.,Federal University of Santa Catarina |
Verdi M.,Federal University of Santa Catarina |
Schlemper Junior B.R.,University of West Santa Catarina |
Caponi S.,Federal University of Santa Catarina
Archives of Medical Research | Year: 2014
The Declaration of Helsinki (DoH), adopted by the World Medical Association (WMA), is one of the most influential international documents in research ethics, is turning 50 in 2014. Its regular updates, seven versions (1975, 1983, 1989, 1996, 2000, 2008, 2013) and two notes of clarification (2002, 2004), characterize it as a 'live' document. The seventh version of the DoH was amended by the 64th WMA General Assembly, Fortaleza, Brazil, October 2013. The new version was reorganized and restructured, with paragraphs subdivided and regrouped. However, the DoH remains controversial and some ethical issues are still uncovered. The major problem was the insertion of the phrase 'less effective than the best proven' on placebo paragraph in order to allow double standard in medical research in low-resource countries. The DoH is a 'live' document, which will continually have to deal with new topics and challenges. Health equity needs to be a priority, and with that, a single ethical standard for medical research. © 2014 IMSS.
Trott A.,University of West Santa Catarina |
Trott A.,Federal University of Rio Grande do Sul |
Houenou L.J.,Forsyth Technical Community College
Recent Patents on DNA and Gene Sequences | Year: 2012
Autosomal dominant spinocerebellar ataxias (SCAs) are a complex group of debilitating and neurodegenerative diseases that affect the cerebellum and its main connections and characterized by a generalized incoordination of gait, speech, and limb movements. In general, the onset of SCAs occurs during adult life and shows great clinical heterogeneity. Currently, the mutations responsible for different types of SCAs have been localized in different regions of the genome, and most of them were already mapped and cloned. Several pieces of evidence suggest that all these diseases share the same molecular mechanism and physiopathological processes. CAG trinucleotide expansion is a common mutational basis of several of these disorders. An expanded polyglutamine tract may become a toxic product when located within the coding region of the gene. The SCA genes, recent patents and the molecular aspects of these disorders are presented in this review. Our knowledge of the molecular mechanisms of SCAs is rapidly expanding, and the development of important studies is bringing hope for effective therapies. © 2012 Bentham Science Publishers.
Ruviaro C.F.,Federal University of Rio Grande do Sul |
Gianezini M.,Federal University of Rio Grande do Sul |
Brandao F.S.,Federal University of Rio Grande do Sul |
Winck C.A.,Federal University of Rio Grande do Sul |
And 2 more authors.
Journal of Cleaner Production | Year: 2012
Worldwide demand to set reliable environmental criteria for food and feed products has brought Life Cycle Assessment (LCA) methodologies to agribusiness. This paper describes the results of a search for scientific literature and government documents regarding the application of LCA to agricultural products worldwide, as a way to capture state-of-the-art technology in the field and to identify the trends and drivers for labeling and certification requirements in international markets. Considering the status of Brazilian agriculture, it would be necessary to adapt the LCA tools to the peculiarities of this country's environmental and technological context, regarding the ability to follow current trends in the application of LCA as a tool for analysis of the environmental impact. In Brazil, any effort to develop specific methodologies for both Life Cycle Inventory and Life Cycle Impact Assessment is urgently needed for the country to remain among the leaders of food and feed exporters and would be appreciated by consumers worldwide. © 2011 Elsevier Ltd. All rights reserved.
Borges E.M.,University of West Santa Catarina
Journal of chromatographic science | Year: 2015
Free silanols on the surface of silica are the "villains", which are responsible for detrimental interactions of those compounds and the stationary phase (i.e., bad peak shape, low efficiency) as well as low thermal and chemical stability. For these reasons, we began this review describing new silica and hybrid silica stationary phases, which have reduced and/or shielded silanols. At present, in liquid chromatography for the majority of analyses, reversed-phase liquid chromatography is the separation mode of choice. However, the needs for increased selectivity and increased retention of hydrophilic bases have substantially increased the interest in hydrophilic interaction chromatography (HILIC). Therefore, stationary phases and this mode of separation are discussed. Then, non-silica stationary phases (i.e., zirconium oxide, titanium oxide, alumina and porous graphitized carbon), which afford increased thermal and chemical stability and also selectivity different from those obtained with silica and hybrid silica, are discussed. In addition, the use of these materials in HILIC is also reviewed. © Crown copyright 2014.
Schwarz R.G.,University of West Santa Catarina
Journal of Educational, Cultural and Psychological Studies | Year: 2016
From the point of view of international law, what we are seeking in this paper is to expose some of the premises for a (new) approach to fundamental social rights. If we think about the absolute supremacy of human life, a life that, to be understood as such, must be a life lived with dignity, we have to think about life from a material point of view and, therefore, in a priority status to the so-called «social» rights, since social rights (economic, social and cultural) address issues as basic to life and human dignity as food, health, shelter, work, education and water. With this understanding, it becomes very clear that the materiality of human dignity rests on the so-called «existential minimum», the hard kernel of social rights, in such a way that social rights are genuine (true) fundamental human rights. Recognition of social rights cannot be, therefore, a mere listing of good intentions on the part of the state. Social rights are fundamental rights, which are for all men, can be exercised by everyone and are essential to life and human dignity. What we are seeking in this paper, then, is to shed light on the understanding that social rights are fundamental human rights in international law. © 2016, Edizioni Universitarie di Lettere Economia Diritto. All rights reserved.
Andrades C.,University of West Santa Catarina
O Papel | Year: 2015
Results of the survey presented in this study aim at evaluating the feasibility of increasing from 45 to 55 the kappa number of Pinus taeda kraft pulp meant for the production of flexible packaging with low grammage paper. The yield observed for kappa number 55 increased by 1.5[%], with consequent increase in shives content, condition that could be handled in the screening process. The tear resistance did not differ between pulps kappa 45 and 55. Tensile strength and airflow through sheet values decreased with the increased kappa number, requiring a higher re(ning action to recondition these properties. The results indicate that kappa 55 pulp can be used for the production of low weight sacks paper, being suitable for the demands associated with this class of products.
Borges E.M.,University of West Santa Catarina |
Rostagno M.A.,University of Campinas |
Meireles M.A.A.,University of Campinas
RSC Advances | Year: 2014
The need for increased throughput and superior performance has increased the demand for stationary phases with improved kinetic performance. Among them, increasing the sample throughput of the ever-growing number of necessary (routine) analyses has become a popular target for reducing precious time. For the past thirty years, high-performance liquid chromatography (HPLC) has been the leading technology when it comes to various analyses; however, the requirement of typically 10-45 min for serial analyses has been a sample throughput-limiting barrier. Recently, the fundamentals of HPLC have been exploited to develop new technologies that can speed up analyses to ground-breaking limits without compromising separation efficiency. This paper reviews the most promising technologies, including porous sub-2 μm ultra-performance liquid chromatography (UPLC) and fused-core particle technology, which have the potential to take LC to the next level. As each analytical method has its own demands, the advances of the above mentioned technologies are discussed for different applications where high throughput analysis can be meaningful. Moreover, we discuss and compare the perspectives of these technologies. © 2014 the Partner Organisations.