Presidente Prudente, Brazil

University of West Paulista
Presidente Prudente, Brazil
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3-Monochloropropane-1,2-diol (3-MCPD) is a food contaminant that can be formed during the thermic processing of various foodstuffs. Studies of reproductive toxicology of 3-MCPD are mainly concentrated in the evaluation of possible insults caused by exposure of adult animals. However, the prepuberty might be a period of different susceptibility to chemicals. The aim of this study was to evaluate the effects on reproductive endpoints of the 3-MCPD-exposure prepubertal male rats. Wistar male rats were assigned to 4 groups: control and exposed to 2.5; 5 or 10 mg kg-1 day-1 of 3-MCPD for 30 days by gavage. Testis and epididymis were used for sperm counts and histology analysis. Sertoli cell number and dynamic of the spermatogenesis were evaluated. Sperm were collected from the vas deferens for evaluation of the sperm motility and morphology. Number of sperm with progressive movement, number of Sertoli cells and germ cells and relative daily sperm production were decreased in the groups exposed to 5 and 10 mg kg-1 day-1 of 3-MCPD. Sperm morphology, testicular and epididymal histology were comparable among groups. Results show that 3-MCPD-exposure of rats from prepuberty might cause alterations in spermatogenesis and sperm maturation, similarly to exposure in adulthood. © 2017, Eduem - Editora da Universidade Estadual de Maringa. All rights reserved.

Ribeiro L.F.P.,University Estadual Of Santa Cruz | Lima M.C.S.,University of West Paulista | Gobatto C.A.,São Paulo State University
Journal of Science and Medicine in Sport | Year: 2010

The slope of the distance-time relationship from maximal 200 and 400 m bouts (S200-400) has been increasingly employed for setting training intensities in swimming. However, physiological and mechanical responses at this speed are poorly understood. Thus, this study investigated blood lactate, heart rate (HR), stroke rate (SR), stroke length (SL) and RPE responses to an interval swimming set at S200-400 in trained swimmers. In a 50-m pool, twelve athletes (16.5 ± 1.2 yr, 176 ± 7 cm, 68.4 ± 5.4 kg, and 7.8 ± 2.5% body fat) performed maximal 200 and 400 m crawl trials for S200-400 determination (1.28 ± 0.05 m/s). Thereafter, swimmers were instructed to perform 5 × 400 m at this speed with 1.5 min rest between repetitions. Three athletes could not complete the set (exhaustion at 21.0 ± 3.1 min). For the remaining swimmers (total set duration = 32.0 ± 1.3 min) significant increases (p < 0.05) in blood lactate (5.7 ± 0.8-7.9 ± 2.4 mmol/l), SR (29.6 ± 3.2-32.1 ± 4.1 cycles/min), HR (169 ± 11-181 ± 8 bpm) and RPE (13.3 ± 1.6-16.3 ± 2.6) were observed through the IS. Conversely, SL decreased significantly (p < 0.05) from the first to the fifth repetition (2.48 ± 0.22-2.31 ± 0.24 m/cycle). These results suggest that interval swimming at S200-400 represents an intense physiological, mechanical and perceptual stimulus that can be sustained for a prolonged period by most athletes. © 2008 Sports Medicine Australia.

Lastoria J.C.,Paulista University | de Abreu M.A.M.M.,University of West Paulista
Anais Brasileiros de Dermatologia | Year: 2014

Leprosy is a chronic infectious condition caused by Mycobacterium leprae (M. leprae). It is endemic in many regions of the world and a public health problem in Brazil. Additionally, it presents a wide spectrum of clinical manifestations, which are dependent on the interaction between M. leprae and host, and are related to the degree of immunity to the bacillus. The diagnosis of this disease is a clinical one. However, in some situations laboratory exams are necessary to confirm the diagnosis of leprosy or classify its clinical form. This article aims to update dermatologists on leprosy, through a review of complementary laboratory techniques that can be employed for the diagnosis of leprosy, including Mitsuda intradermal reaction, skin smear microscopy, histopathology, serology, immunohistochemistry, polymerase chain reaction, imaging tests, electromyography, and blood tests. It also aims to explain standard multidrug therapy regimens, the treatment of reactions and resistant cases, immunotherapy with bacillus Calmette-Guérin (BCG) vaccine and chemoprophylaxis. © 2014 by Anais Brasileiros de Dermatologia.

Prestes-Carneiro L.E.,University of West Paulista | Prestes-Carneiro L.E.,Hospital Ipiranga
HIV/AIDS - Research and Palliative Care | Year: 2013

An increasing number of HIV-infected women of childbearing age are initiating antiretroviral therapy (ART) worldwide. This review aims to discuss updated data of the eligible ART regimens and their role in inducing birth defects in utero. Zidovudine and lamivudine plus a non-nucleoside reverse-transcriptase inhibitor or protease inhibitor (PI) is the first-line regimen applied. The role of zidovudine exposition monotherapy or associated with other ART in inducing birth defects remains inconclusive. The main organ systems involved are genitourinary and cardiovascular. For HIV-infected pregnant women, World Health Organization (WHO) guidelines up to 2010 recommend the same group of drugs that are prescribed to nonpregnant women. The exception is efavirenz, which has been associated with an increase in the risk of teratogenicity. Increased rates of birth defects were found in large cohorts and computational studies conducted recently in infants exposed to efavirenz-containing regimens. The combination of zidovu ine and lamivudine and lopinavir/ritonavir is one of the most used ART regimens for prevention of mother-to-child-transmission. Conflicting data about the role of PI exposure in utero and birth defects have been reported. However, a reduced number of studies evaluating the role of PI in inducing birth defects in women are available. An association between prematurity and PI exposure in pregnancy was extensively described. Some questions arise due to the tendency of initiating ART early in the life of HIV-infected individuals or those at risk of infection. Long-time exposure to different ART regimens and the potential effect of birth-defect induction in pregnancy are not completely understood. Developing regions harbor the highest numbers of women of reproductive age exposed to ART. Most of the largest and expressive data come from developed countries, and could not be sufficiently representative of pregnant women living in developing countries. © 2013 Prestes-Carneiro, publisher and licensee Dove Medical Press Ltd.

Vitolo H.F.,University of West Paulista | Souza G.M.,University of West Paulista | Silveira J.A.G.,Federal University of Ceará
Environmental and Experimental Botany | Year: 2012

The concept of hierarchical levels and its implications for biological organisation have been recognised for many years, and various hierarchical models of biological organisation have been proposed. However, the increased tendency to analyse biological systems from the molecular perspective has overshadowed the integrated view of living beings. On the other hand, plant ecophysiological studies have shown that variations in environmental factors can affect the plant from the molecular to the whole-plant level in different ways. The main objective of this study was to demonstrate that a cross-scale multivariate approach is important to support more consistent conclusions about plant-environment interactions. A study with two tropical crops (a C 3 and a C 4 species) was performed to test the effects, in a single experiment, of high temperatures across different levels of plant organisation from the molecular to the whole-plant level. Simulations using principal component analysis (PCA) on variables grouped according to the nature of the data were performed to investigate the effect of the observational scale on the interpretation of plant responses to high temperature. Despite an efficient photosynthetic response to elevated temperature, indicating an acclimatisation of the photosynthetic apparatus, the results of growth analysis showed a significant reduction in the biomass of both species and in leaf area. The resulting PCAs showed that the distinct differences between the responses of each species to the two temperature regimes were dependent on the specific data set observed. Despite causal associations between the physiological variables in different temperature regimes, the PCA results demonstrate that focusing on specific datasets markedly influences the interpretation of plant environmental response. Thus, we suggest that the multivariate approach could improve the differentiation between a global disruptive disturbance and a local physiological adjustment within a plant. © 2012 Elsevier B.V.

Carvalho C.L.D.B.,University of West Paulista
Anais Brasileiros de Dermatologia | Year: 2014

The tumor necrosis factor alpha is a cytokine related to immune and inflammatory processes by acting on different parts of the body. It is secreted by several cell types including macrophages, lymphocytes, mono-cytes, neutrophils, dendritic cells, among others. Infliximab is a chimeric monoclonal antibody that specifically binds to soluble and transmembrane tumor necrosis factor alpha form blocking its action. In rheumatoid arthritis it is used because the cytokines that cause inflammation in this disease are regulated by tumor necrosis factor alpha and IL-1. We report the case of a 46-year-old patient with rheumatoid arthritis who developed segmental vitiligo after two months using infliximab. The event aims to alert to the existence of this adverse effect that can be induced with the use of this medication. © 2014 by Anais Brasileiros de Dermatologia.

Mendes F.B.R.,University of West Paulista | Hissa-Elian A.,University of Serra dos Orgaos | De Abreu M.A.M.M.,University of West Paulista | Goncalves V.S.,University of West Paulista
Anais Brasileiros de Dermatologia | Year: 2013

Dermatitis herpetiformis (DH) or Duhring-Brocq disease is a chronic bullous disease characterized by intense itching and burning sensation in the erythematous papules and urticarial plaques, grouped vesicles with centrifuge growth, and tense blisters. There is an association with the genotypes HLA DR3, HLA DQw2, found in 80-90% of cases. It is an IgA-mediated cutaneous disease, with immunoglobulin A deposits appearing in a granular pattern at the top of the dermal papilla in the sublamina densa area of the basement membrane, which is present both in affected skin and healthy skin. The same protein IgA1 with J chain is found in the small intestinal mucosa in patients with adult celiac disease, suggesting a strong association with DH. Specific antibodies such as antiendomysium, antireticulina, antigliadin and, recently identified, the epidermal and tissue transglutaminase subtypes, as well as increased zonulin production, are common to both conditions, along with gluten-sensitive enteropathy and DH. Autoimmune diseases present higher levels of prevalence, such as thyroid (5-11%), pernicious anemia (1-3%), type 1 diabetes (1-2%) and collagen tissue disease. The chosen treatment is dapsone and a gluten-free diet. © 2013 by Anais Brasileiros de Dermatologia.

Cardozo R.B.,University of West Paulista | de Araujo F.F.,University of West Paulista
Revista Brasileira de Engenharia Agricola e Ambiental | Year: 2011

This work aimed to evaluate the control of root-knot nematodes and sugarcane growth in function of Bacillus subtilis in soil after multiplication in vinasse. Laboratory tests were developed to define the best concentration of vinasse on the composition of culture medium to optimize the growth of B. subtilis. For the experiment in the greenhouse, soil collected in the area of sugarcane cultivation was used. The following treatments were established: control, vinasse (50 m3 ha-1), Bacillus subtilis in aqueous suspension (50 m3 ha-1); B. subtilis multiplied in vinasse (50 and 100 m3 ha-1). The multiplication of Bacillus subtilis in pure vinasse (25%) was significantly superior when compared to nutrient solution. The application of B. subtilis in aqueous solution promoted growth of sugarcane and reduced reproduction of the nematodes in the roots during the experiment. The use of B. subtilis, multiplied in vinasse did not achieve the benefits to promote growth and control of nematodes in sugarcane, found with the application of only bacteria in soil.

Solubilization of phosphorus, soil correction and corn yield after addition of phosphates and natural and composted tannery sludge. The objective of this study was to evaluate the effect of tannery sludge associated with fosforita (rock phosphate) on the availability of phosphorus and correction of soil, leaf levels and maize yield. The experiment was conducted on the field during twelve months (October 2005 to October 2006) with the incorporation of 2.5 and 5.0 Mg ha-1 of tannery sludge (natural and composted) and 100 kg of P2O5 ha-1 in the form of soluble and natural phosphate (fosforita). During the months of November 2005 to April 2006, maize was grown in the experimental area. Treatment with tannery sludge naturally, at a dose of 5.0 Mg ha-1 associated with fosforita, incremented the levels of phosphorus, calcium and base saturation in the soil for 360 days after implementation. This treatment provided increases in levels of phosphorus in the leaves of maize. The composted tannery sludge treatment, compared to natural tannery sludge, was deficient in the absorption of phosphorus by maize and soil correction. Increased yield of maize was verified by the incorporation of fosforita associated with the natural and composted tannery sludge at a dose of 5.0 Mg ha-1.

Lastoria J.C.,São Paulo State University | de Abreu M.A.M.M.,University of West Paulista
Anais Brasileiros de Dermatologia | Year: 2014

Leprosy is caused by Mycobacterium leprae and has been known since biblical times. It is still endemic in many regions of the world and a public health problem in Brazil. The prevalence rate in 2011 reached 1.54 cases per 10,000 inhabitants in Brazil. The mechanism of transmission of leprosy consists of prolonged close contact between susceptible and genetically predisposed individuals and untreated multibacillary patients. Transmission occurs through inhalation of bacilli present in upper airway secretion. The nasal mucosa is the main entry or exit route of M. leprae. The deeper understanding of the structural and biological characteristics of M. leprae, the sequencing of its genome, along with the advances in understanding the mechanisms of host immune response against the bacilli, dependent on genetic susceptibility, have contributed to the understanding of the pathogenesis, variations in the clinical characteristics, and progression of the disease. This article aims to update dermatologist on epidemiological, clinical, and etiopathogenic leprosy aspects. © 2014 by Anais Brasileiros de Dermatologia.

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