Ramos A.C.,State University of Norte Fluminense |
Dobbss L.B.,University Of Vila Velha Uvv |
Santos L.A.,Federal Rural University of Rio de Janeiro |
Fernandes M.S.,Federal Rural University of Rio de Janeiro |
And 3 more authors.
Applied Adhesion Science | Year: 2015
Background: The humic acid (HA) fraction of soil organic matter (SOM) exerts an effective plant growth promotion through a complex mechanism involving a coordinated activation of several key ion transport and signaling systems. We investigated the effects of HA on H+ and Ca2+ cellular dynamics at the early stages of lateral plant root development. Results: Emergence of lateral root in rice seedlings were related to specific H+ and Ca2+ fluxes in the root elongation zone underlying an activation of the plasma membrane H+-ATPase and of the Ca2+-dependent protein kinase (CDPK). The latter was coupled with an increased expression of the voltage-dependent OsTPC1 Ca2+channels and two stress responsive CDPK isoforms, such as OsCPK7 and OsCPK17. Conclusions: HA act as molecular elicitors of H+ and Ca2+ fluxes, which seem to be upstream of a complex CDPK cell-signaling cascade. These findings shed light on the first ion signaling events involved in the mechanism of action of HA on plant growth and development. © 2015, Ramos et al.; licensee Springer. All Right Reserved.
da Silva Junior V.L.,Federal University of Viçosa |
Donzele J.L.,Federal University of Viçosa |
de Oliveira R.F.M.,Federal University of Viçosa |
Saraiva A.,Federal University of Viçosa |
And 2 more authors.
Ciencia Rural | Year: 2014
The experiment was conducted to evaluate five nutritional plans based on sequences of standardized ileal digestible lysine: 0.90-0.80-0.70, 1.00-0.90-0.80, 1.10-1.00-0.90, 1.20-1.10-1.00, and 1.30-1.20-1.10% fed to gilts from 60 to 99, 129 to 100, and 130 to 148 days of age, respectively. Eighty commercial hybrid gilts, selected for lean gain, with initial weight of 23.46±0.27kg were allotted in a randomized block design, with five treatments, eight replicates, and two pigs per experimental unit. No effect (P>0.05) of the nutritional plans was verified on daily feed intake, daily weight gain and feed conversion. The nutritional plans had no influence (P>0.05) on any of the carcass traits evaluated (carcass yield, meat amount, and meat yield). The nutritional plan of 0.90-0.80-0.70% standardized ileal digestible lysine fed to gilts from 60 to 99, 100 to 129, and 130 to 148 days of age, respectively, meets the standardized ileal digestible lysine requirements of gilts from 60 to 148 days of age. © 2014, Universidade Federal de Santa Maria. All rights reserved.
Paganotti M.A.,University Of Vila Velha Uvv |
Valim V.,Federal University of Espirito Santo |
Serrano E.V.,Escola Superior de Ciencias da Santa Casa de Misericordia de Vitoria Emescam |
Serrano E.V.,Federal University of Espirito Santo |
And 3 more authors.
Revista Brasileira de Reumatologia | Year: 2015
Objective: To carry out the cross-cultural adaptation of EULAR Sjögren's Syndrome PatientReported Index (ESSPRI) for Portuguese language and evaluate its psychometric properties.Method: Cross-sectional study of patients with primary Sjögren's syndrome (SS). The psy-chometric properties (intraobserver reproducibility and construct validity) were studied. Inconstruct validity, ESSPRI was compared with the Patient's Global Assessment (PGA), Profileof Fatigue and Discomfort (Profad), Sicca Symptoms Inventory (SSI) and Functional Assess-ment of Chronic Illness Therapy (Facit-F). Statistical tests used were: Cronbach's alpha,intraclass correlation coefficient (ICC), Bland-Altman method and Spearman coefficient.A value of p = 0.05 was considered significant.Results: There was no difference between versions in both languages; thus, a Brazilian con-sensual version was obtained. All subjects were women aged 49.4 ± 11.6 years, with onsetof symptoms of 7.2 ± 5.4 years, and time of diagnosis of 3.0 ± 3.3 years. The mean ESSPRIwas 6.87 ± 1.97. The intraobserver reproducibility was high and significant (0.911) and, withBland-Altman method, there was no systematic bias in the agreement of measures amongevaluations. A moderate correlation of ESSPRI with all tested instruments was observed. © 2015 Elsevier Editora Ltda.
Marcato D.G.,University Of Vila Velha Uvv |
Sampaio J.D.,University Of Vila Velha Uvv |
Alves E.R.B.,University Of Vila Velha Uvv |
de Jesus J.S.A.,University Of Vila Velha Uvv |
And 3 more authors.
Arquivos Brasileiros de Endocrinologia e Metabologia | Year: 2014
Objective: Sitting height (SH) is an important parameter in the evaluation of children with growth and pubertal disorders. Besides this, it has been viewed as a biomarker of cardiovascular risk, which is increased in adults with relatively short legs. So, the aim of this study was to evaluate the relationship between body proportions and cardiovascular risk markers in children. Subjects and methods: Eight hundred and seventeen children aged 6-13 years were evaluated. Weight, height, sitting-height (SH), sitting-height/height (SH/H), body mass index (BMI) and blood pressure (BP) were assessed and converted to standard deviation scores (SDS) for age and sex. Statistical analyses were performed. Results: There was a positive association of BMI SDS with SH and SH/H SDS (p < 0.001). Overweight children showed SH 0.8 SDS superior to eutrophic children (p < 0.001). SH SDS was also directly related to BP SDS, but this association was not independent of the association between obesity and BP when assessed by multiple regression analyzes. Conclusion: Measures of SH are strongly associated with BMI and BP in children, although the association between SH and BP is probably dependent on the association of both those variables with BMI. This is (an) important information for correct interpretation of SH values in children. © ABE&M todos os direitos reservados.
PubMed | Federal University of Espirito Santo, Escola Superior de Ciencias da Santa Casa de Misericordia de Vitoria Emescam and University Of Vila Velha Uvv
Type: Journal Article | Journal: Revista brasileira de reumatologia | Year: 2015
To carry out the cross-cultural adaptation of Eular Sjgrens Syndrome Patient Reported Index (ESSPRI) for Portuguese language and evaluate its psychometric properties.Cross-sectional study of patients with primary Sjgrens syndrome (SS). The psychometric properties (intraobserver reproducibility and construct validity) were studied. In construct validity, ESSPRI was compared with the Patients Global Assessment (PGA), Profile of Fatigue and Discomfort (Profad), Sicca Symptoms Inventory (SSI) and Functional Assessment of Chronic Illness Therapy (Facit-F). Statistical tests used were:Cronbachs alpha, intraclass correlation coefficient (ICC), Bland-Altman method and Spearman coefficient. A value of p 0.05 was considered significant.There was no difference between versions in both languages; thus, a Brazilian consensual version was obtained. All subjects were women aged 49.4 11.6 years, with onset of symptoms of 7.2 5.4 years, and time of diagnosis of 3.0 3.3 years. The mean ESSPRI was 6.87 1.97. The intraobserver reproducibility was high and significant (0.911) and, with Bland-Altman method, there was no systematic bias in the agreement of measures among evaluations. A moderate correlation of ESSPRI with all tested instruments was observed.The Brazilian Portuguese version of ESSPRI is a valid and reproducible version.
Kuster R.M.,Federal University of Rio de Janeiro |
Kuster R.M.,Federal University of Espirito Santo |
Caxito M.L.C.,State University of Rio de Janeiro |
Sabino K.C.C.,State University of Rio de Janeiro |
And 7 more authors.
Phytochemistry Letters | Year: 2015
Euphorbia tirucalli aerial parts are popularly used in Brazil for cancer treatment. The elution of the aqueous extract of the plant on silica gel C-18 cartridge furnished a water-soluble fraction, which was analyzed directly into the electrospray ionization (ESI) source combined with Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) and characterized as a mixture of malic acid glycosides 1-5. The compounds were detected in their deprotonated form [M-H]-, where their exact mass (mass error lower than 1 ppm), molecular formula (CnHhOo), double bond equivalent (DBE) and connectivity were determined from ESI-(-)-MS and ESI-(-)-MS/MS experiments. The presence of malic acid and glucose, as part of the structures, could originate from crassulacean acid metabolism (CAM) of the plant © 2015 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved.
Camponez M.O.,University Of Vila Velha Uvv |
Salles E.O.T.,Federal University of Espirito Santo |
Filho M.S.,Federal University of Espirito Santo
IEEE Latin America Transactions | Year: 2016
In a previous paper we have proposed two new superresolution image reconstruction algorithms, based on a non-parametric numerical integration Bayesian inference method, the Integrated Nested Laplace Approximation (INLA). Despite achieving superior image reconstruction results compared to other state-of-the-art methods, such algorithms manipulate huge matrices (although sparse). Therefore, the demand for memory usage and computation is high. In this paper, review such algorithms, solving these problems through relaxing one equation in the original mathematical model and involving the high-resolution (HR) image in a Torus. The result is a meaningful reduction in the computation cost of such algorithms and in the dimensions of the matrices handled as well (from n2-by-n2 to n-by-n, the size of the HR image). The result is a new algorithm, much faster than its previous version and other meaningful state-of-the-art algorithms. © 2003-2012 IEEE.
Aguiar N.O.,State University of Norte Fluminense |
Medici L.O.,Federal Rural University of Rio de Janeiro |
Olivares F.L.,State University of Norte Fluminense |
Dobbss L.B.,University Of Vila Velha Uvv |
And 4 more authors.
Annals of Applied Biology | Year: 2016
Water deficit is the major yield-limiting factor for sugarcane crop production that can be enhanced by inoculating with plant growth promoting bacteria (PGPB) combined with humic substances. The aim of this work was to examine changes to the metabolic profile and antioxidant enzyme activity of sugarcane treated with PGPB and humic acid (HA) after drought and then rehydration. The drought was imposed by withholding irrigation for 21 days thereby measuring enzyme activity, metabolic profile and photosynthetic rate 1 week after rehydratation. Growth of plants treated with HA, PGPB and with both treatments combined (PGPB + HA) was higher than control plants. The antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) activities remained higher after rehydration only in plants treated with HA. Plants treated with HA and PGPB + HA exhibited increased transpiration, stomatal conductance and net photosynthesis than plants treated with PGPB. The PGPB-treated plants exhibited drought resistance that resembled 'delayed stress onset', which is a new term for preserving water in the plants tissues. Water preservation in plants treated with PGPB was corroborated by higher relative water content (RWC) than control plants at the end of the drought period. Plants treated with HA + PGPB exhibited the highest water potential after rehydration and high RWC. Osmotic adjustment in the other treatments (control, HA and PGPB) was indicated by a new pattern of metabolic response after rehydration, including generally enhanced carbohydrates and proteins and specific changes induced by HA-enhancing aromatic compounds, whereas PGPB exhibited enhanced fatty acids and other aliphatic H species. Humic acids assist with drought stress recovery by inducing antioxidant enzyme activity whereas PGPB induced preservation of leaf water potential and RWC by closing stomata efficiently, resulting in plant water preservation. © 2015 Association of Applied Biologists.
Tozetti P.B.,University Of Vila Velha Uvv |
Pinto F.E.,University Of Vila Velha Uvv |
Pereira Neto E.,University Vila Velha |
Andrade T.U.,University Of Vila Velha Uvv |
And 2 more authors.
Journal of Morphological Sciences | Year: 2015
Introduction: A differential blood cell count represents a substantial component of the daily clinical routine. The aim of this study was to conduct automated high-throughput analyses of light microscopy images of leukocytes with unspecific staining (hematoxylin-eosin, HE) and to classify the resulting subgroups (lymphocytes, monocytes and neutrophils). Materials and Method: The software package CellProfiler was used for the image analysis and cell classification. The results were compared to those from hematological laboratories that analyzed the same samples. Results: There was a high similarity between the results obtained by image analysis and those from the hematological laboratories, with an r2=0.95 for the differential count and an r2=0.89 for the total count. Conclusion: Differential blood cell count using light microscopy, nonspecific dyes and advanced image analysis is accurate, reproducible, and less expensive compared to other techniques. The results also showed a strong correlation when compared to traditional methods. © 2015 Brazilian Society of Anatomy. All rights reserved.
Anesthetic induction and recovery time of Centropomus parallelus exposed to the essential oil of Aloysia triphylla [Indução e recuperação anestésica de Centropomus parallelus expostos ao óleo essencial de Aloysia triphylla]
Parodi T.V.,Federal University of Santa Maria |
Dos Santos C.A.,University Of Vila Velha Uvv |
Veronez A.,University Of Vila Velha Uvv |
Gomes L.C.,University Of Vila Velha Uvv |
And 2 more authors.
Ciencia Rural | Year: 2016
This study analyzed the effectiveness of the essential oil of Aloysia triphylla (EOA) as an anesthetic and stress reducing agent in the transport of fat snook (Centropomus parallelus). Juveniles were exposed to different concentrations of EOA (seawater-adapted: 25-300µL L-1; freshwater-adapted: 200µL L-1) to identify the anesthetic induction and recovery times. In an additional experiment, seawater-adapted fat snook were transported in plastic bags with 10 or 20µL L-1 EOA for 6 or 24h. The increased concentration of EOA proportionally decreased the time required for anesthesia induction. Mortality was 30 and 70% in fat snook transported with 10 and 20µL L-1, respectively, 24h after transport. The addition of 20µL L-1 EOA significantly reduced plasma cortisol levels during transport compared to the control group, but increased blood glucose levels after 6 and 12h of transport. The EOA reduced total ammonia and dissolved oxygen levels at the end of transport compared to control fish. The EOA is recommended for sedation (25-50µL L-1) and anesthesia (100-300µL L-1) but not for transport of fat snook because it increased mortality. © 2016, Universidade Federal de Santa Maria. All rights reserved.