University of Veterinary Medicine in Kosice
Kosice, Slovakia

The University of Veterinary Medicine and Pharmacy in Košice is the only institution in the Slovak Republic offering higher education in the field of veterinary medicine. It has been providing the veterinary education in English language for 15 years. Nowadays the University offers two English study programmes: 6-year degree programme for secondary school graduates and 4-year degree post-BSc. programme for bachelors in agricultural, veterinary or biological science, both leading to the degree of Doctor of Veterinary Medicine .The DVM qualification was recognised and accredited by the EAEVE European Association of Establishments for Veterinary Education in 1996 and 2002.Students receiving a DVM degree from UVM Kosice, are entitled to automatic membership of the Royal College of Veterinary Surgeons upon returning to the UK, and can thereby practice veterinary medicine in the UK . Wikipedia.

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Herich R.,University of Veterinary Medicine in Kosice
Food and Agricultural Immunology | Year: 2017

IgA antibodies belong to the most important humoral immune factors present on mucosal surfaces. Different defence mechanisms are involved in the permanent and effective surveillance of mucosal surfaces. The robustness of local immunity homeostasis may also explain how the intestinal immune system is able to accommodate a wide range of microbes that have evolved a wide variety of intestinal lifestyles. Bacterial behaviours depend not only on the bacterial species, but also on the host. Commensal bacteria have been directly associated with the proper development of gut-associated lymphoid tissues. © 2016 Informa UK Limited, trading as Taylor & Francis Group.

Hassan S.T.S.,University of Veterinary And Pharmaceutical Sciences Brno | Zemlicka M.,University of Veterinary Medicine in Kosice
Archiv der Pharmazie | Year: 2016

Inhibition of the metalloenzyme urease has important pharmacologic applications in the field of antiulcer and antigastric cancer agents. Urease is involved in many serious infections caused by Helicobacter pylori in the gastric tract as well as by Proteus and related species in the urinary tract. Although numerous studies have described several novel urease inhibitors (UIs) used for the treatment of gastric and urinary infections, all these compounds have exhibited severe side effects, toxicity, and instability. Therefore, to overcome such problems, it is necessary to search for new sources of UIs, such as natural products, that provide reduced side effects, low toxicity, greater stability, and bioavailability. As limited studies have been conducted on plant-derived UIs, this paper aims to highlight and summarize the most promising compounds isolated and identified from plants, such as terpenoids, phenolic compounds, alkaloids, and other substances with inhibitory activities against plant and bacterial ureases; these are in vitro and in vivo studies with an emphasis on structure–activity relationship studies and types of inhibition that show high and promising levels of anti-urease activity. This will aid medicinal chemists in the design and synthesis of novel and pharmacologically potent UIs useful for the development of antiulcer drugs. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Kania B.F.,Warsaw University of Life Sciences | Sutiak V.,University of Veterinary Medicine in Kosice
Research in Veterinary Science | Year: 2011

A different role of L-type antagonists for voltage-gated calcium channels (VGCC) has been previously identified in different types of experimental and clinical pain in man and animals. Present study examined the role of VGCC blocker - diltiazem administered icv (0.25, 0.5, 1.0 and/or 2.0mg in toto) on the development of pain related symptoms, clinical signs, plasma catecholamine level and the inhibition of reticulo-rumen motility caused by 5min lasting mechanical duodenum distension (DD) in the sheep. Experimental DD was conducted by insertion (during surgery) of rubber balloon into the duodenum and the distension by 40ml of warm water. Duodenal distension resulted in a significant increase of behavioural pain responses, tachycardia, hyperventilation, inhibition of reticulo-rumen contractions rate (from 85% to 45% during 15-20min), an increase of plasma catecholamine concentration (over sevenfold increase of epinephrine during 2h following DD, two-times norepinephrine and 84% increase of dopamine). Diltiazem infusion given 10min before DD decreased intensity of visceral nocifensive responses such as: behavioural changes, tachycardia, hyperventilation, reticulo-rumen motility and efficiently prevented appearance of catecholamine release. These data demonstrated that the development and persistence of acute duodenal pain depends on the activation of Ca2+ ion flux leading to neurotransmitters release and modulation of membrane excitability. It seems that diltiazem given icv 10min prior to DD (as a source of acute visceral pain), inhibited specific receptors α1 subunits of VGCCs in target tissues, prevent depolarization of cell membranes and release of neurotransmitters responsible for pain sensitivity in sheep. The observed antinociceptive action of VGCCs type-L blockers suggests that these channels play a crucial role in the modulation of acute visceral pain in sheep. © 2010.

Mudronova D.,University of Veterinary Medicine in Kosice
Beneficial Microbes | Year: 2015

Selection of appropriate bacterial strains is crucial for development of new probiotic preparations. The fundamental prerequisite for potential efficacy of a probiotic preparation for oral application is the selection of appropriate bacterial strains with good gastrointestinal colonisation abilities, antimicrobial activity, and tolerance of conditions in the gastrointestinal tract, resistance to different antimicrobial agents, survival during processing and storage. The strain should be genetically stable, it should have good growth properties, to maintain its high viability at processing and when in storage. Mostly, the properties of promising strains are tested in the first phase in vitro, and only the best ones undergo subsequent in vivo testing. in vitro tests are often performed by classical microbiological cultivation methods which are material and time consuming, and they are not able to distinguish between 'viable but nonculturable' and dead bacteria. Flow cytometry is usually used for counting, phenotyping or functional characterisation of immune cells. Nowadays, flow cytometry is increasingly used in microbiology for counting bacteria, determining their viability and metabolic activity, detecting specific strains or testing their adherence abilities. The utilisation of flow cytometry in combination with an appropriate fluorescent labelling represents an effective and rapid method for the selection of probiotic bacteria.

Petrovova E.,University of Veterinary Medicine in Kosice
Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes | Year: 2011

The histological structure of rabbit spleen after bendiocarb administration was studied. Bendiocarb was perorally administered for 30 days. At day 10, 20 and 30 morphometric analysis was realized. Quantitative evaluation showed that in the control group the relative spleen volume of white pulp ranged from 35.03 ± 10.94 % and the relative volume of red pulp 64.97 ± 10.94 %. In all experimental groups were detected significantly higher relative volume of red pulp and the lower relative volume of white pulp, except on day 30. The experimental groups showed a significant increase in the number of lymphocytes in comparison with the control group. On day 10 we observed a significant increase in diameter of investigated lymphocytes. The results of our study determined structural alterations in spleen structure after bendiocarb administration, which probably causes alteration in the immune system.

Schusterova I.,Childrens Faculty Hospital | Leenen F.H.H.,University of Ottawa | Jurko A.,Pediatrics Cardiology Clinic | Sabol F.,East Slovak Institute of Cardiovascular Disease | Takacova J.,University of Veterinary Medicine in Kosice
Pediatric Obesity | Year: 2014

What is already known about this subject The prevalence of childhood obesity has increased markedly in the past 2 decades. Abdominal fat is a better predictor of risk than body mass index. Waist circumference (WC) as a measure of abdominal fat has limited sensitivity and specificity. What this study adds Epicardial adipose tissue (EAT) as measured by echocardiography represents a simple and reliable marker of visceral adiposity. In children, both body mass index and EAT show a similar or better correlation with markers of cardiometabolic risk than does waist circumference. Background Epicardial adipose tissue (EAT) is the visceral fat deposit around the heart and is commonly increased in obese subjects. EAT is related to cardiometabolic risk factors and non-alcoholic fatty liver disease (NAFLD) in adults, but this relationship is not well known in children. Objectives Echocardiographic assessment of EAT and its association with cardiometabolic risk factors in overweight and obese children. Study groups and methods In 25 (mean age 13.0-‰±-‰2.3) overweight and obese subjects and 24 lean controls, blood pressure (BP), WC, fasting plasma glucose and insulin, lipids, uric acid and hepatic enzymes were measured. EAT thickness was measured by transthoracic echocardiography. Results In overweight and obese subjects, EAT was significantly higher compared to normal weight children. Overweight and obese children had significantly higher body mass index (BMI), WC, BP, triglycerides (TAG), low-density lipoprotein and total cholesterol, hepatic enzymes alanine aminotransferase (ALT) and γ-glutamyl transferase, and lower high-density lipoprotein cholesterol (HDL-C). EAT correlated significantly with BP, TAG, uric acid, HDL-C, apoprotein B and ALT. Correlation coefficients were similar or better than for WC, but similar or lower than for BMI. Conclusion EAT thickness in children is associated with an unfavourable cardiometabolic risk profile including biochemical signs of NAFLD and hyperuricaemia, but is not a stronger indicator than BMI. © 2012 The Authors.

Leskova V.,University of Veterinary Medicine in Kosice
Acta virologica | Year: 2013

In this study, a major part of genome of the pestivirus isolate 297 from Slovakia, comprising the 7195 nt-long 5'-UTR-NS3 region was sequenced and analyzed. Conserved cleavage sites between individual viral proteins of this region were determined and the number of amino acids of respective proteins was estimated as follows: 168 for Npro, 100 for C, 227 for Erns, 195 for E1, 373 for E2, 70 for p7, 453 for NS2, and 683 for NS3. Based on sequence and phylogenetic analysis of 5'-UTR, Npro, and E2 the isolate 297 was characterized as a border disease virus of genotype 3. It was found to be distinct from other BDV-3 strains analyzed so far, consequently forming a distinct branch within the phylogenetic clade. All these data expand a relatively limited knowledge of genetic properties of individual BDV genotypes and strains circulating in the Central Europe.

Petrovova E.,University of Veterinary Medicine in Kosice
Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes | Year: 2010

Various pesticides have immuno-suppressive effects, and thus the organisms become responsive to viral, bacterial and parasitic diseases and neoplasm. The aim of the study was to observe the structure of the small intestine (height of enterocytes and crypts), mucosal lymphoid tissue (Payer's patches, lymphocytes in lamina propria) and a lymph node after administration of bendiocarb (2,2-dimethyl-1,3-benzodioxol-4-yl-methylcarbamate) on days 3, 10, 20, 30 and 60 of the experiment. The height of the observed enterocytes showed an increasing tendency. On days 20, 30 and 60 we also observed an increase in diameter of crypts located in intestinal epithelium. The number of cells in lamina propria mucosae was significantly reduced on days 20 and 30 after administration of bendiocarb. Observations of the lymph node showed that on days 10 and 20 there was a significant increase in relative volume of medulla at the expense of the relative volume of the cortex and a decrease in the number of lymphocytes. However, we recorded an increase in diameter of lymphocytes. The intracellular parasite Toxoplasma gondii (T. gondii) belongs to the most common pathogenic parasites in the world and it can cause serious health complications in pregnant and immunodeficient individuals. DNA isolation, standard polymerase chain reaction (PCR) and visualization in a 2.5 % agarose gel, the presence of DNA T. gondii was detected in no examined rabbit brain samples. Using real time PCR T. gondii DNA was detected and quantified in the three rabbit brain samples (10 %).

Hurnikova Z.,Slovak Academy of Sciences | Hurnikova Z.,University of Veterinary Medicine in Kosice | Miterpakova M.,Slovak Academy of Sciences | Mandelik R.,University of Veterinary Medicine in Kosice
Parasitology Research | Year: 2013

Angiostrongylus vasorum is a metastrongyloid nematode that may cause cardiopulmonary disease, neurological signs and coagulopathies in dogs. The parasite has an indirect life cycle with molluscs as intermediate hosts, in which the infective third larval stage develops. Recently, A. vasorum has been repeatedly reported in dogs outside the endemic areas, indicating that this parasite is widely distributed over Europe. This is the first record of an autochthonous infection in a dog from Slovakia that was casually diagnosed during routine preventive parasitological examination. A. vasorum first-stage larvae were recovered using the Baermann technique and identified by length and characteristic tail morphology. The animal originated from Slovakia and had not travelled abroad. The dog had been regularly walked on grass fields with a concentrated presence of common species of Gastropoda and frogs. The owner reported that the dog had been licking and eating grass and it had shown curiosity for molluscs and frogs. The first finding of A. vasorum-infected dog in Slovakia has confirmed that the parasite is spreading beyond the traditional hyperendemic foci, which accentuates the need for monitoring and increasing of disease awareness in primary care clinical practice. © 2013 Springer-Verlag Berlin Heidelberg.

Valencakova A.,University of Veterinary Medicine in Kosice | Halanova M.,University of P.J. Šafarik
Comparative Immunology, Microbiology and Infectious Diseases | Year: 2012

The microsporidia are emerging agents of infectious disease in both immunocompromised and immunocompetent mammals. Recently, there has been an increased interest in studying the immunobiology of microsporidiosis. This paper discusses the humoral and cell-mediated immune responses to Encephalitozoon spp. The T-cell-mediated responses appear to be most important in conferring resistance. This has become evident by the lethal effects of microsporidiosis in T-cell-deficient hosts. However, much still needs to be learned about the immunobiology of microsporidiosis regarding the specific T-cell responses and the cytokines that provide protective immunity and facilitate the macrophage-mediated killing of microsporidia. Such information will become important in developing immunotherapeutic strategies to control microsporidiosis in the future. © 2011 Elsevier Ltd.

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