Time filter

Source Type

Stergioti E.,National and Kapodistrian University of Athens | Deligeoroglou E.,National and Kapodistrian University of Athens | Economou E.,National and Kapodistrian University of Athens | Tsitsika A.,National and Kapodistrian University of Athens | And 4 more authors.
Gynecological Endocrinology | Year: 2013

Anorexia nervosa is a serious eating disorder that is associated with decreased bone mineral density and greater lifetime risk for fractures. This case-controlled study, analyzed single nucleotide polymorphisms of genes encoding vitamin D receptor, estrogen receptor alpha (ESR1), collagen type I and calcitonin receptor (CTR). Relationships between genotype and body mass index, cycling status and lumbar spine bone mineral density (LBMD) were determined in 40 adolescent girls with anorexia nervosa and 10 age-matched controls. The distribution of CTR-AluI genotypes differed between groups, but this polymorphism was not associated with LBMD Z-score. Distribution of ESR1-XbaI genotypes did not differ between groups, but the AA genotype was associated with decreased LBMD Z-score (≤-1) (OR = 24.79, 95% CI, 1.01-606.08). Carriers of the A allele were more likely to have decreased LBMD Z-scores compared with carriers of the G allele (OR = 4.12, 95% CI, 1.23-13.85, p = 0.022). In conclusion, our study shows that anorexic patients with wild-type genotype ESR-XbaI receptor are in greater risk for decreased BMD in relation to those with the mutated gene. Prompt recognition of these patients is crucial because early administration of the proper therapeutic treatment may contribute to the prevention of adverse sequelae on bone metabolism. © 2013 Informa UK Ltd.


Papagiannis D.,University of Thessaly | Rachiotis G.,University of Thessaly | Symvoulakis E.K.,Private Practice | Daponte A.,University of Thessalia Medical School | And 3 more authors.
Journal of Multidisciplinary Healthcare | Year: 2013

Background: There are still sparse data on vaccination coverage against human papillomavirus (HPV) among students in the health professions. The aim of this study was to investigate HPV vaccination coverage in female students from the health professions in Greece. Methods: A self-administered, anonymous questionnaire was distributed to second-year and third-year female students pursuing degrees in medicine, nursing, and paramedical health disciplines in central Greece. Results: Overall vaccination coverage was 44.3%. The major reason for lack of vaccination was fear about safety of the vaccine. Participants who had received information about safety of the vaccine from the mass media and paramedical students had lower vaccination coverage in comparison with students who had received information about vaccine safety from alternative sources. Conclusion: Further quantitative and qualitative research is needed to design educational activities targeting female students in the health professions in order to create a positive domino effect and improve HPV vaccination coverage levels in Greece. © 2013 Papagiannis et al.


Giannoukas A.D.,University of Thessalia Medical School
Phlebolymphology | Year: 2013

Superficial thrombophlebitis is a manifestation of thrombosis that involves the superficial venous system of the lower limb. It is frequently underreported and is considered an insignificant entity. In some cases it may coexist with deep vein thrombosis, or may extend from the superficial system to the deep veins, increasing the risk of complications such as pulmonary embolism. Diagnosis by ultrasound scanning is essential to exclude deep venous thrombosis and confirm the extent of the superficial thrombophlebitis. When superficial thrombophlebitis coexists with deep vein thrombosis, or when the main trunk of the saphenous veins in the vicinity of the junctions is affected, treatment with low molecular weight heparins should be initiated. © 2013 Les Laboratoires Servier-All rights reserved throughout the world and in all languages.


Athanassiou E.,University of Thessalia Medical School | Vamvakopoulou D.N.,University of Thessalia Medical School | Zacharoulis D.,University Hospital of Larissa | Paroutoglou G.,University of Thessalia Medical School | And 4 more authors.
Applied Immunohistochemistry and Molecular Morphology | Year: 2010

Objectives: To assess the prognostic value of combined mismatch DNA repair (MMR) phenotyping in 2 synchronous histomorphologically distinct gastric adenocarcinomas (GADCs), each accompanied by gastrointestinal stromal tumors (GISTs) of the proximal small bowel. Summary Background Data: A 72-year-old female and a 55-year-old male patient were submitted to partial and total gastrectomy, respectively, with synchronous resection of a GIST in the proximal small bowel. The 2 patients attained contrasting survival outcomes. The female survives disease-free 20 months after surgery having received no chemotherapy. The male who received adjuvant chemotherapy developed metastases in liver and lung, and died 18 months after surgery. Methods: We phenotype MSH2 and MLH1 protein expression in tumor relative to matched normal tissue by immunohistochemistry. Results: Immunohistochemistry analysis revealed different combined MMR phenotypes for the 2 histomorhologically distinct GADCs and similar for both GISTs studied. Conclusions: Good and bad prognosis for disease-free survival of patients based on reduced and elevated combined MMR phenotypic expression of the 2 histomorphologically distinct GADCs, could be explained by disease-associated emergence of genomic MMR alterations in the tumor. The impact of synchronous GISTs with common intermediate MMR phenotypes on patient survival is rather incidental and secondary to predominating GADCs. Copyright © 2010 by Lippincott Williams & Wilkins.


Dafopoulos K.,University of Thessalia Medical School | Vrekoussis T.,University of Crete | Chalvatzas N.,University of Thessalia Medical School | Messini C.I.,University of Thessalia Medical School | And 4 more authors.
Fertility and Sterility | Year: 2013

Objective To investigate the effect of metformin administration on the expression of endometrial corticotrophin-releasing hormone (CRH) and urocortin (UCN) in the midluteal phase of the cycle. Design Experimental study, performed in 2010-2011. Setting University hospital. Patient(s) Eight healthy, normally cycling and parous women volunteered for the study. Intervention(s) All women were investigated in two nonconsecutive cycles (control cycle, untreated and after one cycle break; trial cycle, oral administration of metformin [850 mg × 2]). Endometrial pipelle biopsies were obtained on day LH+7. Main Outcome Measure(s) The endometrial biopsies were immunohistochemically assessed for CRH and UCN expression. Evaluation of positivity was performed by applying the immunoreactive score. Result(s) Compared with samples from control cycles, CRH and UCN were significantly reduced in endometrial samples obtained during metformin treatment. This down-regulation was significant both in the endometrial cells and in the endometrial stroma. Conclusion(s) This is the first study showing that during the midluteal phase of the cycle, metformin may decrease the production of CRH and UCN in the endometrium. Metformin interference to decidualization could happen by CRH/UCN modification. © 2013 by American Society for Reproductive Medicine.


Daponte A.,University of Thessalia Medical School | Pournaras S.,University of Thessalia Medical School | Polyzos N.P.,University of Thessalia Medical School | Tsezou A.,University of Thessalia Medical School | And 4 more authors.
Journal of Clinical Endocrinology and Metabolism | Year: 2011

Context: Diagnosis of early pregnancy failure is hampered by the lack of reliable serological markers. Objective: We assessed whether a single serum measurement of placental growth factor (PlGF) and the soluble Flt-1 (sFlt-1) receptor of vascular endothelial growth factor at 6-8 wk gestation could differentiate failed pregnancies, whether ectopic pregnancies (EP) or missed abortions (MA), from healthy intrauterine pregnancies (IUP). Design and Setting: We conducted a prospective clinical study at a tertiary university hospital between January 2009 and February 2011. Patients: A total of 78 consecutive patients (38 EP, 40 MA) with failed early pregnancy and 50 IUP (control group) participated in the study. Intervention(s): Determination of serum PlGF and sFlt-1 has been carried out by ELISA. Gene expression of PlGF and Flt-1 in trophoblasts was performed by RT-PCR. Main Outcome Measure(s): We investigated whether a single, combined serum measurement of the above markers could contribute to the differential diagnosis. Results: PlGF and sFlt-1 concentration was lower in both EP (mean, 14.60 ± 3.42/178.16 ± 76.03 pg/ml) and MA (mean, 16.25 ± 4.73/399.42 ± 337.54 pg/ml) compared to IUP (mean, 21.64 ± 5.68/1390.32 ± 655.37 pg/ml). sFlt-1 (P = 0.033) and sFlt-1/PlGF ratio (P = 0.029) but not PlGF had the ability to discriminate MA from EP. Compared to women with viable IUP, mRNA gene expression levels of PlGF and Flt-1 were considerably lower in women with MA and in women with EP. Conclusions: Combined measurement of sFlt-1 and PlGF levels can differentiate normal from failed pregnancies, whereas sFlt-1 as well as sFlt-1/PlGF ratio can also discriminate EP from MA. PlGF and Flt-1 gene expression in trophoblasts from women with EP and MA appears impaired. Copyright © 2011 by The Endocrine Society.


Perrakis N.,Metaxa Cancer Memorial Hospital | Athanassiou E.,University of Thessalia Medical School | Vamvakopoulou D.,University of Thessalia Medical School | Kyriazi M.,Metaxa Cancer Memorial Hospital | And 3 more authors.
World Journal of Gastroenterology | Year: 2011

AIM: To study the outcome of patients undergoing sur- gical resection of the bowel for sustained radiation-induced damage intractable to conservative management. METHODS: During a 7-year period we operated on 17 cases (5 male, 12 female) admitted to our surgical department with intestinal radiation injury (IRI). They were originally treated for a pelvic malignancy by surgical resection followed by postoperative radiotherapy. During follow-up, they developed radiation enteritis requiring surgical treatment due to failure of conservative management. RESULTS: IRI was located in the terminal ileum in 12 patients, in the rectum in 2 patients, in the descending patients had resection of the affected region(s). There were no postoperative deaths, while 3 cases presented with postoperative complications (17.7%). All patients remained free of symptoms without evidence of recurrence of IRI for a median follow-up period of 42 mo (range, 6-96 mo). CONCLUSION: We report a favorable outcome without IRI recurrence of 17 patients treated by resection of the diseased bowel segment. © 2011 Baishideng. All rights reserved.


Klontzas M.E.,University of Crete | Zibis A.H.,University of Thessalia Medical School | Karantanas A.H.,University of Crete
American Journal of Roentgenology | Year: 2015

OBJECTIVE. Osteoid osteoma is a benign bone tumor that is commonly located in the proximal femur. Although CT is helpful in the diagnosis of osteoid osteoma, patients in whom the clinical presentation of the tumor is atypical are first referred to undergo MRI, which yields a high percentage of false-negative or inconclusive results. We sought to explore the association between a half-moon sign of bone marrow edema and the presence of osteoid osteoma of the femoral neck. SUBJECTS AND METHODS. The MRI examinations of 11 consecutive patients with osteoid osteoma (group 1) were prospectively included in the study. In addition, 950 consecutive hip MRI examinations of 485 patients were retrospectively analyzed for the presence of bone marrow edema of the femoral neck, with 19 patients identified as having bone marrow edema resulting from causes other than osteoid osteoma (group 2). Patients in both study groups were evaluated for the presence of the half-moon sign. RESULTS. The half-moon sign of bone marrow edema was significantly associated with the presence of osteoid osteoma (p < 0.0001), having been seen on the MR images of all 11 patients in group 1 and only one of 19 patients in group 2. MRI examination was therefore 94.7% specific and 100% sensitive for the detection of osteoid osteoma, with positive and negative predictive values of 91.7% and 100%, respectively. CONCLUSION. The half-moon sign is an MRI finding that is highly specific and sensitive for an accurate diagnosis of osteoid osteoma of the femoral neck. © American Roentgen Ray Society.


PubMed | University of Thessalia Medical School
Type: Case Reports | Journal: Applied immunohistochemistry & molecular morphology : AIMM | Year: 2010

To assess the prognostic value of combined mismatch DNA repair (MMR) phenotyping in 2 synchronous histomorphologically distinct gastric adenocarcinomas (GADCs), each accompanied by gastrointestinal stromal tumors (GISTs) of the proximal small bowel.A 72-year-old female and a 55-year-old male patient were submitted to partial and total gastrectomy, respectively, with synchronous resection of a GIST in the proximal small bowel. The 2 patients attained contrasting survival outcomes. The female survives disease-free 20 months after surgery having received no chemotherapy. The male who received adjuvant chemotherapy developed metastases in liver and lung, and died 18 months after surgery.We phenotype MSH2 and MLH1 protein expression in tumor relative to matched normal tissue by immunohistochemistry.Immunohistochemistry analysis revealed different combined MMR phenotypes for the 2 histomorhologically distinct GADCs and similar for both GISTs studied.Good and bad prognosis for disease-free survival of patients based on reduced and elevated combined MMR phenotypic expression of the 2 histomorphologically distinct GADCs, could be explained by disease-associated emergence of genomic MMR alterations in the tumor. The impact of synchronous GISTs with common intermediate MMR phenotypes on patient survival is rather incidental and secondary to predominating GADCs.


PubMed | University of Thessalia Medical School
Type: Journal Article | Journal: The Journal of clinical endocrinology and metabolism | Year: 2011

Diagnosis of early pregnancy failure is hampered by the lack of reliable serological markers.We assessed whether a single serum measurement of placental growth factor (PlGF) and the soluble Flt-1 (sFlt-1) receptor of vascular endothelial growth factor at 6-8 wk gestation could differentiate failed pregnancies, whether ectopic pregnancies (EP) or missed abortions (MA), from healthy intrauterine pregnancies (IUP).We conducted a prospective clinical study at a tertiary university hospital between January 2009 and February 2011.A total of 78 consecutive patients (38 EP, 40 MA) with failed early pregnancy and 50 IUP (control group) participated in the study.Determination of serum PlGF and sFlt-1 has been carried out by ELISA. Gene expression of PlGF and Flt-1 in trophoblasts was performed by RT-PCR.We investigated whether a single, combined serum measurement of the above markers could contribute to the differential diagnosis.PlGF and sFlt-1 concentration was lower in both EP (mean, 14.60 3.42/178.16 76.03 pg/ml) and MA (mean, 16.25 4.73/399.42 337.54 pg/ml) compared to IUP (mean, 21.64 5.68/1390.32 655.37 pg/ml). sFlt-1 (P = 0.033) and sFlt-1/PlGF ratio (P = 0.029) but not PlGF had the ability to discriminate MA from EP. Compared to women with viable IUP, mRNA gene expression levels of PlGF and Flt-1 were considerably lower in women with MA and in women with EP.Combined measurement of sFlt-1 and PlGF levels can differentiate normal from failed pregnancies, whereas sFlt-1 as well as sFlt-1/PlGF ratio can also discriminate EP from MA. PlGF and Flt-1 gene expression in trophoblasts from women with EP and MA appears impaired.

Loading University of Thessalia Medical School collaborators
Loading University of Thessalia Medical School collaborators