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Pimenta Bueno, Brazil

Figueredo F.G.,Laboratory of Microbiology and Molecular Biology | Lucena B.F.F.,Laboratory of Microbiology and Molecular Biology | Tintino S.R.,Laboratory of Microbiology and Molecular Biology | Matias E.F.F.,Laboratory of Microbiology and Molecular Biology | And 7 more authors.
Pharmaceutical Biology | Year: 2014

Context: The combination of antibiotics with natural products has demonstrated promising synergistic effects in several therapeutic studies. Objective: The aim of this study was to determine the effect of a combination of an ethanol extract of Myracrodruon urundeuva Fr. All. (Anacardiaceae) (aroeira plant) and its essential oil with six antimicrobial drugs against multiresistant strains of Staphylococcus aureus and Escherichia coli from clinical isolates. Materials and methods: After identification of the chemical components by GC-MS, the antibacterial activity of the natural products and antibiotics was assessed by determining the minimal inhibitory concentration (MIC) using the microdilution method and concentrations ranging 8-512μg/mL and 0.0012-2.5mg/mL, respectively. Assays were performed to test for a possible synergistic action between the plant products and the antimicrobials, using the extract and the oil at a sub-inhibitory concentration (128μg/mL) and antibiotic at concentrations varying between 8 and 512μg/mL. Results: The GC-MS analysis identified the main compound as δ-carene (80.41%). The MIC of the natural products was >1024μg/mL, except against S. aureus ATCC25923. Only the combinations of the natural products with gentamicin, amikacin and clindamycin were effective against S. aureus 358, enhancing the antibiotic activity by reducing the MIC. Conclusions: The extract from aroeira showed a higher antibacterial activity and the oil was more effective in potentiating the activity of conventional antibiotics. © 2014 Informa Healthcare USA, Inc. All rights reserved. Source


Matias E.F.F.,Laboratory of Microbiology and Molecular Biology | Santos K.K.A.,Laboratory of Microbiology and Molecular Biology | Almeida T.S.,University of the Region of Cariri | Costa J.G.M.,University of the Region of Cariri | And 2 more authors.
Chemotherapy | Year: 2011

Background: Escherichia coli is known to produce enterotoxins whose properties and role in diarrheal disease have been extensively investigated; besides, this bacterium is related to several extra-intestinal problems in the intensive care unit and in surgical wound infections. Some species of Staphylococcus are recognized as etiological agents of opportunistic infections in animals and humans. This study is the first test on the modulation of antibiotic activity by Croton campestris A. against multiresistant strains of E. coli and Staphylococcus aureus. Methods: In this study, the hexane and methanol extract of C. campestris A. was tested for antibacterial activity alone and in combination with aminoglycosides against bacterial strains. The synergy of the methanol and hexane extract was verified by microdilution method. Results: A synergistic effect of both extracts combined with the aminoglycosides was demonstrated. Conclusions: It is suggested that the extracts from C. campestris A. could be used as a source of natural product derived from this plant with resistance-modifying antibacterial activity, providing a new weapon against the problem of bacterial resistance to antibiotics. Copyright © 2011 S. Karger AG, Basel. Source


Matias E.F.F.,Leao Sampaio College | Alves E.F.,Leao Sampaio College | Silva M.K.N.,Leao Sampaio College | Carvalho V.R.A.,Leao Sampaio College | And 7 more authors.
Industrial Crops and Products | Year: 2016

Cordia verbenacea DC (Boraginaceae), popularly known as "Erva Baleeira," has been widely studied with respect to its chemical and pharmacological properties, and its anti-inflammatory, analgesic and anti-ulcerogenic activities have been demonstrated. The objective of this study was to determine the essential oil content with regard to time of collection of leaves and to evaluate the antibacterial activity and antibiotic-modifying activity of the oil, sabinene and sabinene hydrate against multidrug-resistant strains. GC/MS analysis showed that in samples of the essential oil obtained at different times over one year, there was qualitative and quantitative variation in chemical composition, with statistical significance (p <0.001 and p <0.05). The MIC of essential oil, sabinene and sabinene hydrate was ≥1024 μg/mL, demonstrating no clinically relevant antibacterial activity. In tests for modulation of bacterial resistance to aminoglycosides, essential oil, sabinene and sabinene hydrate showed significant changes (p <0.001) in MIC values, enhancing the effect of the antibiotics tested, thereby demonstrating synergistic potential. These results serve as a reference for choosing the best time to obtain the oil according to the constituent of interest, as well as for using the oil and its constituents as modifying agents of bacterial resistance to aminoglycoside. © 2016 Elsevier B.V. Source


Coutinho H.D.M.,University of the Region of Cariri | Costa J.G.M.,University of the Region of Cariri | Costa J.G.M.,Leao Sampaio College | Falcao-Silva V.S.,Federal University of Paraiba | And 2 more authors.
Comparative Immunology, Microbiology and Infectious Diseases | Year: 2010

In this study the ethanol extract (EEMC) of Momordica charantia L. (Cucurbitaceae) was tested for its modifying antibiotic activity against a MRSA strain. The growth of an MRSA (SA358) in the absence and presence of aminoglycosides was evaluated. A potentiating effect between this extract and all aminoglycosides was demonstrated. Similarly, the same effect was shown by chlorpromazine on kanamycin, gentamicin and neomycin, indicating the involvement of an efflux system in the resistance to these aminoglycosides. Extracts from M. charantia could be used as a source of plant-derived natural products with resistance-modifying activity. This is the first report about the modifying antibiotic activity of M. charantia, constituting a new weapon against multi-resistant bacteria such as MRSA. © 2009 Elsevier Ltd. Source


Sousa I.J.B.,University of the Region of Cariri | Matias E.F.F.,University of the Region of Cariri | Menezes I.R.A.,University of the Region of Cariri | Coutinho H.D.M.,University of the Region of Cariri
Journal of Food and Nutrition Research | Year: 2013

Medicinal plants had been used by traditional populations as a way to treat diseases for centuries. Currently, despite the search of the pharmaceutical industry for different kinds of drugs, the bacterial mechanisms of resistance against antimicrobial drugs are a real problem to the health. Origanum vulgare and Coriandrum sativum are medicinal plants with essential oils of a known antibacterial activity. The essential oils were assayed for their antibacterial and modulatory antibiotic activities by gaseous contact method. Both essential oils demonstrated modulatory activity mainly when associated with gentamicin and vancomycin. These natural products demonstrated an interesting potential to be studied in association with antibiotics to elaborate new drug formulations, or to be used as functional foods. © 2013 VÚP Food Research Institute, Bratislava. Source

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