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Matias E.F.F.,Leao Sampaio College | Alves E.F.,Leao Sampaio College | Silva M.K.N.,Leao Sampaio College | Carvalho V.R.A.,Leao Sampaio College | And 7 more authors.
Industrial Crops and Products | Year: 2016

Cordia verbenacea DC (Boraginaceae), popularly known as "Erva Baleeira," has been widely studied with respect to its chemical and pharmacological properties, and its anti-inflammatory, analgesic and anti-ulcerogenic activities have been demonstrated. The objective of this study was to determine the essential oil content with regard to time of collection of leaves and to evaluate the antibacterial activity and antibiotic-modifying activity of the oil, sabinene and sabinene hydrate against multidrug-resistant strains. GC/MS analysis showed that in samples of the essential oil obtained at different times over one year, there was qualitative and quantitative variation in chemical composition, with statistical significance (p <0.001 and p <0.05). The MIC of essential oil, sabinene and sabinene hydrate was ≥1024 μg/mL, demonstrating no clinically relevant antibacterial activity. In tests for modulation of bacterial resistance to aminoglycosides, essential oil, sabinene and sabinene hydrate showed significant changes (p <0.001) in MIC values, enhancing the effect of the antibiotics tested, thereby demonstrating synergistic potential. These results serve as a reference for choosing the best time to obtain the oil according to the constituent of interest, as well as for using the oil and its constituents as modifying agents of bacterial resistance to aminoglycoside. © 2016 Elsevier B.V.


Coutinho H.D.M.,University of the Region of Cariri | Costa J.G.M.,University of the Region of Cariri | Costa J.G.M.,Leao Sampaio College | Falcao-Silva V.S.,Federal University of Paraiba | And 2 more authors.
Comparative Immunology, Microbiology and Infectious Diseases | Year: 2010

In this study the ethanol extract (EEMC) of Momordica charantia L. (Cucurbitaceae) was tested for its modifying antibiotic activity against a MRSA strain. The growth of an MRSA (SA358) in the absence and presence of aminoglycosides was evaluated. A potentiating effect between this extract and all aminoglycosides was demonstrated. Similarly, the same effect was shown by chlorpromazine on kanamycin, gentamicin and neomycin, indicating the involvement of an efflux system in the resistance to these aminoglycosides. Extracts from M. charantia could be used as a source of plant-derived natural products with resistance-modifying activity. This is the first report about the modifying antibiotic activity of M. charantia, constituting a new weapon against multi-resistant bacteria such as MRSA. © 2009 Elsevier Ltd.


Matias E.F.F.,Laboratory of Microbiology and Molecular Biology | Santos K.K.A.,Laboratory of Microbiology and Molecular Biology | Almeida T.S.,University of the Region of Cariri | Costa J.G.M.,University of the Region of Cariri | And 2 more authors.
Chemotherapy | Year: 2011

Background: Escherichia coli is known to produce enterotoxins whose properties and role in diarrheal disease have been extensively investigated; besides, this bacterium is related to several extra-intestinal problems in the intensive care unit and in surgical wound infections. Some species of Staphylococcus are recognized as etiological agents of opportunistic infections in animals and humans. This study is the first test on the modulation of antibiotic activity by Croton campestris A. against multiresistant strains of E. coli and Staphylococcus aureus. Methods: In this study, the hexane and methanol extract of C. campestris A. was tested for antibacterial activity alone and in combination with aminoglycosides against bacterial strains. The synergy of the methanol and hexane extract was verified by microdilution method. Results: A synergistic effect of both extracts combined with the aminoglycosides was demonstrated. Conclusions: It is suggested that the extracts from C. campestris A. could be used as a source of natural product derived from this plant with resistance-modifying antibacterial activity, providing a new weapon against the problem of bacterial resistance to antibiotics. Copyright © 2011 S. Karger AG, Basel.


PubMed | University of the Region of Cariri and Federal University of Paraiba
Type: Journal Article | Journal: Journal of young pharmacists : JYP | Year: 2014

In this work, the ethyl acetate and hexane fractions of Pityrogramma calomelanos (L.) were evaluated to antibacterial and antifungal activity against strains of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans, Candida krusei and Candida tropicalis.The study was performed aiming to assess the antimicrobial effect with the method of dilution in HIA. The hexane and ethyl acetate fractions were named HFPC and EAFPC, respectively.Both fractions of specie P.calomelanos displayed good activity against S.aureus when associated with gentamicin. When associated with the antifungal, the fractions did not exhibit relevant activity against species of Candida.These results indicate that this specie can be used as a possible source of natural products of antibacterial interest, mainly when combined with aminoglycosides.


Sousa I.J.B.,University of the Region of Cariri | Matias E.F.F.,University of the Region of Cariri | Menezes I.R.A.,University of the Region of Cariri | Coutinho H.D.M.,University of the Region of Cariri
Journal of Food and Nutrition Research | Year: 2013

Medicinal plants had been used by traditional populations as a way to treat diseases for centuries. Currently, despite the search of the pharmaceutical industry for different kinds of drugs, the bacterial mechanisms of resistance against antimicrobial drugs are a real problem to the health. Origanum vulgare and Coriandrum sativum are medicinal plants with essential oils of a known antibacterial activity. The essential oils were assayed for their antibacterial and modulatory antibiotic activities by gaseous contact method. Both essential oils demonstrated modulatory activity mainly when associated with gentamicin and vancomycin. These natural products demonstrated an interesting potential to be studied in association with antibiotics to elaborate new drug formulations, or to be used as functional foods. © 2013 VÚP Food Research Institute, Bratislava.


Souza T.M.,University of the Region of Cariri | Braga M.F.B.M.,University of the Region of Cariri | Costa J.G.M.,University of the Region of Cariri | Saraiva A.A.F.,University of the Region of Cariri | Coutinho H.D.M.,University of the Region of Cariri
International Journal of Phytomedicine | Year: 2012

This study is the first report about the enhancement of additive effect of Pityrogramma caiomeianos L. (link) associated with aminoglycosides against a multiresistant Staphylococcus aureus. In this study, the ethanol extract and the ethyl acetate fraction were assayed to identify the relationship between these natural products and the aminoglycosides using the checkerboard method. The checkerboard assay was realized to verify if the relationship between these drugs and natural products represent a synergistic, additive, indifferent or antagonistic effect. All natural products and antibiotics assayed demonstratetd an additive effect, with FIC index = 0.5. These results indicate that the ethanol extract and the ethyl acetate fraction of P caiomelanos can be a source of plant derived products with antibiotic modifier activity, affecting positively the antibiotic activity of aminoglycosides.


PubMed | University of the Region of Cariri
Type: Journal Article | Journal: Natural product research | Year: 2013

Leishmaniasis is caused by parasites of the genus Leishmania. Recent reports about leishmaniasis show a few number of drugs available, indicating the necessity of new drugs. In this study, the ethanol extract and fractions of Pityrogramma calomelanos (L.) link. (Pteridaceae) were assayed to verify the cytotoxicity and in vitro leishmanicidal activity against promastigote forms of Leishmania brasiliensis. The cytotoxic assay was performed using fibroblasts NCTC929. The studies indicated a leishmanicidal effect of the ethanol extract and the ethyl-acetate fraction. However, a high cytotoxic effect was observed. The hexane and methanol fractions did not show leishmanicidal activity, nor cytotoxic effect. The phytochemical screening detected the presence of alkaloids, a class of secondary metabolites with a known leishmanicidal activity. This is the first study reporting an anti-Leishmania and cytotoxic effect of P. calomelanos, being an interesting approach in the search for drugs against this disease.


PubMed | University of the Region of Cariri
Type: Journal Article | Journal: Pharmaceutical biology | Year: 2010

Bacterial infectious agents represent a risk to populations, where they are responsible for the high morbidity and mortality. In combating these pathogens, our main line of defense is the use of antibiotics. However, the indiscriminate use of these drugs select resistant strains to these same drugs.In this study the ethanol extract of Hyptis martiusii Benth. (EEHM) (Lamiaceae) was tested for its antimicrobial activity against aminoglycoside multi-resistant Staphylococcus aureus (MRSA).In this study, the ethanol extract of H. martiusii was prepared and tested with chlorpromazine for its antimicrobial activity using the microdilution method. Chlorpromazine and the ethanol extract were used alone and also in combination with aminoglycosides against a MRSA strain resistant to these antibiotics to determine the participation of efflux systems in resistance mechanisms. The FIC index was calculated and evaluated by the checkerboard method.A potentiating effect between this extract and aminoglycosides was demonstrated. Similarly, a potentiating effect of chlorpromazine with kanamycin was detected, indicating the involvement of an efflux system in the resistance to this aminoglycoside. The checkerboard method with combinations of aminoglycosides and EEHM demonstrated additive effect with kanamycin and gentamicin. It is therefore suggested that extracts from H. martiusii could be used as a source of plant-derived natural products with resistance- modifying activity.This is the first report about the modifying antibiotic activity of Hyptis martiusii, constituting a new approach against bacterial resistance to antibiotics as aminoglycosides.


PubMed | University of the Region of Cariri
Type: Journal Article | Journal: Chemotherapy | Year: 2011

Escherichia coli is known to produce enterotoxins whose properties and role in diarrheal disease have been extensively investigated; besides, this bacterium is related to several extra-intestinal problems in the intensive care unit and in surgical wound infections. Some species of Staphylococcus are recognized as etiological agents of opportunistic infections in animals and humans. This study is the first test on the modulation of antibiotic activity by Croton campestris A. against multiresistant strains of E. coli and Staphylococcus aureus.In this study, the hexane and methanol extract of C. campestris A. was tested for antibacterial activity alone and in combination with aminoglycosides against bacterial strains. The synergy of the methanol and hexane extract was verified by microdilution method.A synergistic effect of both extracts combined with the aminoglycosides was demonstrated.It is suggested that the extracts from C. campestris A. could be used as a source of natural product derived from this plant with resistance-modifying antibacterial activity, providing a new weapon against the problem of bacterial resistance to antibiotics.


PubMed | University of the Region of Cariri
Type: Journal Article | Journal: Comparative immunology, microbiology and infectious diseases | Year: 2010

In this study the ethanol extract (EEMC) of Momordica charantia L. (Cucurbitaceae) was tested for its modifying antibiotic activity against a MRSA strain. The growth of an MRSA (SA358) in the absence and presence of aminoglycosides was evaluated. A potentiating effect between this extract and all aminoglycosides was demonstrated. Similarly, the same effect was shown by chlorpromazine on kanamycin, gentamicin and neomycin, indicating the involvement of an efflux system in the resistance to these aminoglycosides. Extracts from M. charantia could be used as a source of plant-derived natural products with resistance-modifying activity. This is the first report about the modifying antibiotic activity of M. charantia, constituting a new weapon against multi-resistant bacteria such as MRSA.

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