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Santos R.C.V.,Centro Universitario Franciscano | Alves C.F.D.S.,Centro Universitario Franciscano | Schneider T.,Centro Universitario Franciscano | Lopes L.Q.S.,Centro Universitario Franciscano | And 5 more authors.
Journal of Invertebrate Pathology | Year: 2012

The Gram-positive, spore-forming bacterium Paenibacillus larvae is the primary bacterial pathogen of honeybee brood and the causative agent of American foulbrood disease (AFB). One of the feasible alternative treatments being used for their control of this disease is essential oils. In this study in vitro antimicrobial activity of Andiroba and Copaíba essential oils against Paenibacillus species, including P. larvae was evaluated. Minimal inhibitory concentration (MIC) in Mueller-Hinton broth by the microdilution method was assessed. Andiroba registered MIC values of 1.56-25%, while the MICs values obtained for Copaíba oil were of 1.56-12.5%. In order to determine the time-response effect of essential oils on P. larvae, this microorganism was exposed to the oils for up to 48. h. After 24. h treatment with Andiroba oil and after 48. h treatment with Copaíba oil no viable cells of P. larvae ATCC 9545 were observed. The possible toxic effect of essential oils were assessed by the spraying application method of the same concentrations of MICs. Bee mortality was evident only in treatment with Andiroba oil and the Copaíba oil shows no toxic effects after 10. days of observation. Taking together ours results showed for the first time that these oils presented a high activity against Paenibacillus species showing that Copaíba oil may be a candidate for the treatment or prevention of AFB. © 2011 Elsevier Inc.. Source

Lima L.F.P.,University of the Region of Campanha | Miotto S.T.S.,Federal University of Rio Grande do Sul
Darwiniana | Year: 2011

Pollen morphology of eight species of Cyclanthera and three species of Sicyos was studied using light and scanning electron microscopy. Pollen grains of Cyclanthera have a mean polar diameter of 51.40 μm, and an equatorial mean diameter of 49.10 μm, a prolate spheroidal shape (P/E = 1.11), 4-7 zonocolporate, with circular endoapertures, and punctitegilate exine. Pollen grains of Sicyos have a mean polar diameter of 50.60 μm and a mean equatorial diameter of 61.30 μm, an oblate spheroidal shape (P/E = 0.88), 8-12 colpate, supra and microreticulate echinate exine. Differences among the genera and species are discussed. Source

This study presents original data on the reproduction and feeding ecology of two syntopic amphisbaenians, Amphisbaena munoai Klappenbach, 1969 and Anops kingi Bell, 1833, from southern Brazil. Sampling was carried out from April 2004 to April 2006 at a highland area located in São Jerônimo and Barão do Triunfo municipalities, Rio Grande do Sul, Brazil. A total of 647 amphisbaenians were collected, 510 specimens of A. munoai and 137 specimens of A. kingi, of which 130 and 93, respectively, had their gonads and gut content analyzed. Both species presented a unimodal pattern of seasonal distribution, sexually mature females with significantly larger snout vent length (SVL) than sexually mature males, and seasonal reproductive cycle. Adults of A. kingi had significantly larger (SVL) than those of Amphisbaena munoai. Both species had generalist diets, but termites (Isoptera) was a staple item in their diet. Greater predominance of insect larvae was observed in the diet of A. kingi, which may be due to its larger body in comparison to A. munoai. Insect larvae ingested by A. kingi were significantly larger than those ingested by A. munoai. Diets of adult males and females of both species were not significantly different. The ontogenetic diet comparison in both species revealed significant numerical differences, with adults consuming higher numbers of prey. Source

Sarmento M.B.,Federal University of Pampa | Da Silva A.C.S.,Instituto Biotecnologico Of Reproducao Vegetal Intec | dos Santos K.L.,Federal University of Santa Catarina | de Mattos L.C.P.,University of the Region of Campanha
Revista Brasileira de Fruticultura | Year: 2013

Brazilian guava, native fruit tree from Southern Brazil, has become relevant due to its potencial use as ecological and comercial. This article aims evaluate the physiological quality of Brazilian guava seeds by tetrazolium test. The work was divided in two parts. In the first, both lots were submitted to the germination test, index of speed germination, mean time of germination, emergency of seedlings in greenhouse, mean time of emergency and shoot length of seedlings. In the second part it was performed the tetrazolium test. Two concentrations of tetrazolium were used (0,5 e 1,0) and two times of imbibition (2 h e 4 h). To the lot 2007, the treatment 0,5 TZ 4 h obtained 73% of viable seeds, otherwise, to the lot 2008, the was no significant differences among treatments. For the germination test there has been positive correlation for 0,5 TZ 2 h and 1 TZ 2 h treatments. Emergency of seedlings in greenhouse was correlated only to 0,5 TZ 2 h. The Tetrazolium Test has allowed to rank the lots in four classes of viability, confirming the eficiency of the test to evaluate the viability of Brazilian Guava seeds. Source

Audino L.D.,Federal University of Lavras | da Silva P.G.,Universidadefederal Of Santa Maria | Nogueira J.M.,University of the Region of Campanha | de Moraes L.P.,ONG Instituto Pro Pampa Sustentavel | Vaz-de-Mello F.Z.,Federal University of Mato Grosso
Iheringia - Serie Zoologia | Year: 2011

This study provides data about the Scarabaeinae fauna that occurs in a eucalyptus plantation inserted in an originally open area, describing which are able to establish themselves in this introduced and shaded environment. The survey was carried in an area of eucalyptus monoculture using flight intercept and pitfall traps baited with human feces, rotten banana and carcass, from December 2005 to November 2006. Twenty-eight species were collected, belonging to 11 genera. Of these 28 species, 11 have been previously recorded in grassland and native forest environments of the region, 11 only in grasslands, two were exclusive of eucalyptus plantations and four were not identified at the specific level, which did not allow the achievement of more information on these species in the natural ecosystems of the region. The results showed that species that occur in eucalyptus are habitat generalists, have preference for shaded environments and/or are tourist species. This, however, is one of the few surveys performed in the region. Therefore, additional studies are necessary to obtain more information about the distribution and response of dung beetles to natural and anthropogenic ecosystems of the region. Source

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