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Munkert J.,Friedrich - Alexander - University, Erlangen - Nuremberg | Costa C.,Friedrich - Alexander - University, Erlangen - Nuremberg | Budeanu O.,University of the Academy of Sciences of Moldova | Petersen J.,Friedrich - Alexander - University, Erlangen - Nuremberg | And 4 more authors.
Plant Biology | Year: 2015

This study aimed to define progesterone 5β-reductases (P5βR, EC 1.3.99.6, enone 1,4-reductases) as function-associated molecular markers at the plant family level. Therefore cDNAs were isolated from 25 Brassicaceae species, including two species, Erysimum crepidifolium and Draba aizoides, known to produce cardiac glycosides. The sequences were used in a molecular phylogeny study. The cladogram created is congruent to the existing molecular analyses. Recombinant His-tagged forms of the P5βR cDNAs from Aethionema grandiflorum, Draba aizoides, Nasturtium officinale, Raphanus sativus and Sisymbrium officinale were expressed in E. coli. Enone 1,4-reductase activity was demonstrated in vitro using progesterone and 2-cyclohexen-1-one as substrates. Evidence is provided that functional P5βRs are ubiquitous in the Brassicaceae. The recombinant P5βR enzymes showed different substrate preferences towards progesterone and 2-cyclohexen-1-one. Sequence comparison of the catalytic pocket of the P5βR enzymes and homology modelling using Digitalis lanata P5βR (PDB ID: 2V6G) as template highlighted the importance of the hydrophobicity of the binding pocket for substrate discrimination. It is concluded that P5βR genes or P5βR proteins can be used as valuable function-associated molecular markers to infer taxonomic relationship and evolutionary diversification from a metabolic/catalytic perspective. © 2015 German Botanical Society and Royal Botanical Society of the Netherlands. Source


Ivanov S.,Moldova Academy of Sciences | Ivanov S.,University of the Academy of Sciences of Moldova
International Journal of Computer Mathematics | Year: 2013

An important direction in the research of P systems is paving the way to modelling real-world biological structures. Clock-free P systems represent an attempt at bringing the P formalism closer to biological origins by eliminating global synchronization, the global clock. The goal of this paper is to provide a formalization of clock-free P systems starting from the formal concept of a network of cells. This approach makes it possible to show that clock-free systems can be simulated with the usual networks of cells. © 2013 Copyright Taylor and Francis Group, LLC. Source


University of the Academy of Sciences of Moldova | Entity website

Acte normative Avize Susinerea tezelor Informaii de contact INSTITUTUL DE GEOLOGIE I SEISMOLOGIE AL ACADEMIEI DE TIINE A MOLDOVEI Str . Academiei 3, MD-2028, Chiinu, Republica Moldova Tel: (373) 22 739027, Fax: (373) 22 739729 e-mail: cancelarie ...


University of the Academy of Sciences of Moldova | Entity website

Acte normative Avize Susinerea tezelor Informaii de contact INSTITUTUL DE CHIMIE AL ACADEMIEI DE TIINE A MOLDOVEI Str. Academiei 3, MD-2028, Chiinu, Republica Moldova Tel: (373) 22 725490, (373) 22 725490, Fax: (373) 22 739954 e-mail: ichem@asm ...


University of the Academy of Sciences of Moldova | Entity website

Studenii i profesorii Universitii s-au reunit mari, 28 iunie, ncepnd cu ora 10, pentru a

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