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Sestacova T.,University of the Academy of Sciences of Moldova | Gisca I.,AMG Agroselect Comert Company | Cucereavii A.,AMG Agroselect Comert Company | Port A.,University of the Academy of Sciences of Moldova | Duca M.,University of the Academy of Sciences of Moldova
Biotechnology and Biotechnological Equipment | Year: 2016

Transcriptional activity of four defence-related genes (NPR1, PAL, defensin and PR5) in seven sunflower lines – resistant (MS-2161A), tolerant (MS-2039A) and susceptible (MS-2098A, MS-2091A, MS-2077A, MS-2067A and MS-1589A), infected with three broomrape populations (Tulcea, Romania; Soroca and Anenii Noi, Republic of Moldova), was studied in advanced stages of infection (90 days after sowing). Obtained results revealed that resistant genotypes are characterized through higher stability in transcriptional activity of the studied genes. Thus, resistance could be associated with ability rapidly to maintain and recover a normal level of metabolism under more intensive stress factors. Also, it was established that expression of PR5 and defensin genes was altered and revealed considerable deviations in this phase of adaptation, while NPR1 and PAL mostly showed values at the level of the control group, which allows assuming that these genes, in the moment of sample collection and analysis, had normalized transcriptional activity, probably being involved in early responses. © 2016 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis.


Duca M.,University of the Academy of Sciences of Moldova
Helia | Year: 2015

The first document that attests the cultivation of sunflower on the territory of the Republic of Moldova dates back to 1845. The first evidence of oil production from sunflower seeds has been identified in 1867. Since then, the area cultivated with sunflower has expanded exponentially. Sunflower has now become the third most produced crop, after corn and wheat. In the Republic of Moldova, sunflower research activities have been focused on genetics, breeding and improvement of crop cultivation technologies, with a special emphasis on resistance to disease and pests including broomrape, which can considerably diminish crop productivity. Significant efforts have been invested to identify solutions for fighting parasites, such as the investigation of morpho-physiological, biochemical and genetic aspects of the host- parasite interaction; the development of chemical and agro-technical methods to fight parasites; the evaluation of genetic resistance in artificial and natural conditions; the development of hybrids resistant to a number of Orobanche varieties; and the monitoring of broomrape impact on the production indices. The current paper examines the scientific information available, providing a comprehensive view on the management, breeding, resistance and economic impact of sunflower broomrape in the Republic of Moldova. The review aims to provide a perspective for future research strategies to further develop our understanding of the parasite-host interaction.


Munkert J.,Friedrich - Alexander - University, Erlangen - Nuremberg | Costa C.,Friedrich - Alexander - University, Erlangen - Nuremberg | Budeanu O.,University of the Academy of Sciences of Moldova | Petersen J.,Friedrich - Alexander - University, Erlangen - Nuremberg | And 4 more authors.
Plant Biology | Year: 2015

This study aimed to define progesterone 5β-reductases (P5βR, EC 1.3.99.6, enone 1,4-reductases) as function-associated molecular markers at the plant family level. Therefore cDNAs were isolated from 25 Brassicaceae species, including two species, Erysimum crepidifolium and Draba aizoides, known to produce cardiac glycosides. The sequences were used in a molecular phylogeny study. The cladogram created is congruent to the existing molecular analyses. Recombinant His-tagged forms of the P5βR cDNAs from Aethionema grandiflorum, Draba aizoides, Nasturtium officinale, Raphanus sativus and Sisymbrium officinale were expressed in E. coli. Enone 1,4-reductase activity was demonstrated in vitro using progesterone and 2-cyclohexen-1-one as substrates. Evidence is provided that functional P5βRs are ubiquitous in the Brassicaceae. The recombinant P5βR enzymes showed different substrate preferences towards progesterone and 2-cyclohexen-1-one. Sequence comparison of the catalytic pocket of the P5βR enzymes and homology modelling using Digitalis lanata P5βR (PDB ID: 2V6G) as template highlighted the importance of the hydrophobicity of the binding pocket for substrate discrimination. It is concluded that P5βR genes or P5βR proteins can be used as valuable function-associated molecular markers to infer taxonomic relationship and evolutionary diversification from a metabolic/catalytic perspective. © 2015 German Botanical Society and Royal Botanical Society of the Netherlands.


Ivanov S.,Moldova Academy of Sciences | Ivanov S.,University of the Academy of Sciences of Moldova
International Journal of Computer Mathematics | Year: 2013

An important direction in the research of P systems is paving the way to modelling real-world biological structures. Clock-free P systems represent an attempt at bringing the P formalism closer to biological origins by eliminating global synchronization, the global clock. The goal of this paper is to provide a formalization of clock-free P systems starting from the formal concept of a network of cells. This approach makes it possible to show that clock-free systems can be simulated with the usual networks of cells. © 2013 Copyright Taylor and Francis Group, LLC.


PubMed | Friedrich - Alexander - University, Erlangen - Nuremberg, University of the Academy of Sciences of Moldova and Federal University of Lavras
Type: Journal Article | Journal: Plant biology (Stuttgart, Germany) | Year: 2015

This study aimed to define progesterone 5-reductases (P5R, EC 1.3.99.6, enone 1,4-reductases) as function-associated molecular markers at the plant family level. Therefore cDNAs were isolated from 25 Brassicaceae species, including two species, Erysimum crepidifolium and Draba aizoides, known to produce cardiac glycosides. The sequences were used in a molecular phylogeny study. The cladogram created is congruent to the existing molecular analyses.Recombinant His-tagged forms of the P5R cDNAs from Aethionema grandiflorum, Draba aizoides, Nasturtium officinale, Raphanus sativus and Sisymbrium officinale were expressed in E.coli. Enone 1,4-reductase activity was demonstrated in vitro using progesterone and 2-cyclohexen-1-one as substrates. Evidence is provided that functional P5Rs are ubiquitous in the Brassicaceae. The recombinant P5R enzymes showed different substrate preferences towards progesterone and 2-cyclohexen-1-one. Sequence comparison of the catalytic pocket of the P5R enzymes and homology modelling using Digitalis lanata P5R (PDB ID: 2V6G) as template highlighted the importance of the hydrophobicity of the binding pocket for substrate discrimination. It is concluded that P5R genes or P5R proteins can be used as valuable function-associated molecular markers to infer taxonomic relationship and evolutionary diversification from a metabolic/catalytic perspective.


University of the Academy of Sciences of Moldova | Entity website

Acte normative Avize Susinerea tezelor Informaii de contact INSTITUTUL DE GEOLOGIE I SEISMOLOGIE AL ACADEMIEI DE TIINE A MOLDOVEI Str . Academiei 3, MD-2028, Chiinu, Republica Moldova Tel: (373) 22 739027, Fax: (373) 22 739729 e-mail: cancelarie ...


University of the Academy of Sciences of Moldova | Entity website

Bucurie i entuziasm pentru grupul de studeni, masteranzi, doctoranzi i cercettori, care n


University of the Academy of Sciences of Moldova | Entity website

Seminare, lecii publice Training TEHNICI DE ANALIZ MOLECULAR, 17 - 20 septembrie 2013. Informaii utile n atenia studenilor, masteranzilor ...


University of the Academy of Sciences of Moldova | Entity website

n perioada 2-8 decembrie 2015, Universitatea Academiei de tiine a Moldovei a fost vizitat, de doamna dr. Isgouhi Kaloshian, profesor n Departamentul Genetic i Nematologie, Universitatea California, Riverside, SUA ...


University of the Academy of Sciences of Moldova | Entity website

Studenii i profesorii Universitii s-au reunit mari, 28 iunie, ncepnd cu ora 10, pentru a

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