As Sulaymānīyah, Iraq
As Sulaymānīyah, Iraq

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Daneshvar N.,University Putra Malaysia | Daneshvar N.,University of Manitoba | Rasedee A.,University Putra Malaysia | Shamsabadi F.T.,University Putra Malaysia | And 6 more authors.
Tissue and Cell | Year: 2015

Induced Pluripotent Stem Cells (iPSCs) has been produced by the reprogramming of several types of somatic cells through the expression of different sets of transcription factors. This study consists of a technique to obtain iPSCs from human umbilical cord mesenchymal stem cells (UC-MSCs) in a feeder layer-free process using a mini-circle vector containing defined reprogramming genes, Lin28, Nanog, Oct4 and Sox2. The human MSCs transfected with the minicircle vector were cultured in iPSCs medium. Human embryonic stem cell (ESC)-like colonies with tightly packed domelike structures appeared 7-10 days after the second transfection. In the earliest stages, the colonies were green fluorescence protein (GFP)-positive, while upon continuous culture and passage, genuine hiPSC clones expressing GFP were observed. The induced cells, based on the ectopic expression of the pluripotent markers, exhibited characteristics similar to the embryonic stem cells. These iPSCs demonstrated in vitro capabilities for differentiation into the three main embryonic germ layers by embryoid bodies formation. There was no evidence of transgenes integration into the genome of the iPSCs in this study. In conclusion, this method offers a means of producing iPSCs without viral delivery that could possibly overcome ethical concerns and immune rejection in the use of stem cells in medical applications. © 2015.


Rahman H.S.,University Putra Malaysia | Rahman H.S.,University of Sulaimany | Rasedee A.,University Putra Malaysia | How C.W.,University Putra Malaysia | And 14 more authors.
International Journal of Nanomedicine | Year: 2015

Cancer nanotherapy is progressing rapidly with the introduction of many innovative drug delivery systems to replace conventional therapy. Although the antitumor activity of zerumbone (ZER) has been reported, there has been no information available on the effect of ZER-loaded nanostructured lipid carrier (NLC) (ZER-NLC) on murine leukemia cells. In this study, the in vitro and in vivo effects of ZER-NLC on murine leukemia induced with WEHI-3B cells were investigated. The results from 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, Hoechst 33342, Annexin V, cell cycle, and caspase activity assays showed that the growth of leukemia cells in vitro was inhibited by ZER-NLC. In addition, outcomes of histopathology, transmission electron microscopy, and Tdt-mediated dUTP nick-end labeling analyses revealed that the number of leukemia cells in the spleen of BALB/c leukemia mice significantly decreased after 4 weeks of oral treatment with various doses of ZER-NLC. Western blotting and reverse-transcription quantitative polymerase chain reaction assays confirmed the antileukemia effects of ZER-NLC. In conclusion, ZER-NLC was shown to induce a mitochondrial-dependent apoptotic pathway in murine leukemia. Loading of ZER in NLC did not compromise the anticancer effect of the compound, suggesting ZER-NLC as a promising and effective delivery system for treatment of cancers. © 2015 Rahman et al.


Berzinji A.,University of Stockholm | Abdullah F.S.,University Utrecht | Kakei A.H.,University of Sulaimany
Proceedings - 2013 European Intelligence and Security Informatics Conference, EISIC 2013 | Year: 2013

Nowadays using the Social Networking Sites (SNS) especially Facebook by terrorist groups to spread their ideas among people has grown. In this work we try to monitor those groups by Facebook Operation techniques and then apply an algorithm to detect most active node in the group that can recruit most nodes on Facebook. The algorithm works on centralities to find out the node that is most central in the group. © 2013 IEEE.


Al-Daraji H.J.,University of Baghdad | Salih A.M.,University of Sulaimany
Pakistan Journal of Nutrition | Year: 2012

A total of 300 one day old Ross 308 broiler chicks were used in this study to determine the effect of supplementing ration with different levels of arginine on productive performance of broiler chickens. The chicks were allocated for 4 treatment groups (75 chicks for each group) and each treatment was consisted of five replicates with 15 chicks each. Treatment groups were: C: control group (without any addition of arginine); T1, T2 and T3: adding arginine to the diet of broiler chickens at levels of 0.02, 0.04 and 0.06%, respectively. Two types of diets were used over the period of experiment, starter diet was used from one to 20 days of chicks' age and then grower diet was used till the end of the experiment (46 days of age). Blood traits included in this study were total erythrocytes count (RBC), Packed Cell Volume (PCV), Hemoglobin concentration (Hb), Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Hemoglobin Concentration (MCHC), total leucocytes count (WBC), hetrophil to lymphocyte ratio (H/L) and thrombocytes count (Thr.). Results indicated that adding arginine to the diet of broiler chickens (T1, T2 and T3) resulted in significant increase in RBC, PCV, Hb, MCV, MCH, MCHC, WBC and Thr. and significant decrease in H/L ratio during all periods of experiment and regarding the total means of these traits as compared with control group. In conclusion, supplementation of the broiler ration with L-arginine resulted in significant improvement with respect to blood traits included in this study. Therefore, arginine can be used as effective feed additive for enhancing physiological status of broiler chickens. © Asian Network for Scientific Information, 2012.


Al-Daraji H.J.,University of Baghdad | Salih A.M.,University of Sulaimany
Pakistan Journal of Nutrition | Year: 2012

A total of 300 one day old Ross 308 broiler chicks were used in this study to determine the effect of supplementing ration with different levels of arginine on productive performance of broiler chickens. The chicks were allocated for 4 treatment groups (75 chicks for each group) and each treatment was consisted of five replicates with 15 chicks each. Treatment groups were: C: control group (without any addition of arginine); T1, T2 and T3: adding arginine to the diet of broiler chickens at levels of 0.02, 0.04 and 0.06%, respectively. Two types of diets were used over the period of experiment, starter diet was used from one to 20 days of chicks' age and then grower diet was used till the end of the experiment (46 days of age). Productive traits included in this study were live body weight, weight gain, feed intake, feed conversion ratio, production index, economic figure and livability. Results revealed that feeding diets containing arginine (T1, T2 and T3) resulted in significant increase (p<0.05) in live body weight, weight gain, feed intake and feed conversion ratio during the most period of experiment and with relation to accumulative weight gain, accumulative feed intake and accumulative feed conversion ratio as compared with control group (C). However, adding arginine to the diet of broiler chickens resulted in significant increase (p<0.05) in production index, economic figure and livability in comparison with control group. In conclusion, dietary arginine supplementation resulted in significant improvement in productive traits included in this study. So arginine can be used as effective feed additive for improve productive performance of broiler chickens. © Asian Network for Scientific Information, 2012.

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