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Kuchtova A.,Slovak Academy of Sciences | Janecek S.,Slovak Academy of Sciences | Janecek S.,University of St. Cyril and Methodius in Trnava
Biochimica et Biophysica Acta - Proteins and Proteomics | Year: 2015

Abstract The CAZy glycoside hydrolase (GH) family GH77 is a monospecific family containing 4-α-glucanotransferases that if from prokaryotes are known as amylomaltases and if from plants including algae are known as disproportionating enzymes (DPE). The family GH77 is a member of the α-amylase clan GH-H. The main difference discriminating a GH77 4-α-glucanotransferase from the main GH13 α-amylase family members is the lack of domain C succeeding the catalytic (β/α)8-barrel. Of more than 2400 GH77 members, bacterial amylomaltases clearly dominate with more than 2300 sequences; the rest being approximately equally represented by Archaea and Eucarya. The main goal of the present study was to deliver a detailed bioinformatics study of family GH77 (416 collected sequences) focused on amylomaltases from borreliae (containing unique sequence substitutions in functionally important positions) and plant DPE2 representatives (possessing an insert of ∼ 140 residues between catalytic nucleophile and proton donor). The in silico analysis reveals that within the genus of Borrelia a gradual evolutionary transition from typical bacterial Thermus-like amylomaltases may exist to family-GH77 amylomaltase versions that currently possess progressively mutated the most important and otherwise invariantly conserved positions. With regard to plant DPE2, a large group of bacterial amylomaltases represented by the amylomaltase from Escherichia coli with a longer N-terminus was identified as a probable intermediary connection between Thermus-like and DPE2-like (existing also among bacteria) family GH77 members. The presented results concerning both groups, i.e. amylomaltases from borreliae and plant DPE2 representatives (with their bacterial counterpart), may thus indicate the direction for future experimental studies. © 2015 Elsevier B.V. Source

Puterova Z.,Comenius University | Krutosikova A.,University of St. Cyril and Methodius in Trnava | Veghc D.,Slovak University of Technology in Bratislava
Arkivoc | Year: 2010

Chemistry of 2-aminothiophenes is arguably one of the most extensive and dynamic field of present-day thiophene research. Since 1961 when first report on the Gewald reaction was reported it became a universal method for synthesis of substituted 2-aminothiophenes and has gained prominence in recent times. The availability of reagents and the mild reaction conditions all contribute to the versatility of this reaction. This review summarizes the synthetic strategies for substituted 2-aminothiophenes. Consequently, details about the proposed mechanism of Gewald-like reactions and the wide scope of substituted 2-aminothiophenes for real life applications. © ARKAT USA, Inc. Source

Hegedus O.,University of St. Cyril and Methodius in Trnava | Cepelova D.,Oddelenie chemickych a fyzikalnych analyz | Hegedusova A.,Katedra zeleninarstva and univerzity v Nitre
Chemicke Listy | Year: 2015

A titrimetric method of determination of free and fixed fats in foodstufs after their saponification was compared with a standard extraction method. Both the methods gave the same results in a wide concentration range. The former was less time-consuming and less expensive. © 2015 Czech Society of Chemical Engineering. All rights reserved. Source

Agency: Cordis | Branch: FP7 | Program: CP-IP | Phase: SSH-2010-5.1-1 | Award Amount: 9.97M | Year: 2011

MYPLACE explores how young peoples social participation is shaped by the shadows (past, present and future) of totalitarianism and populism in Europe. Conceptually, it goes beyond the comparison of discrete national political cultures or reified classifications of political heritage (postcommunist/liberal democratic); it is premised rather on the pan-European nature of a range of radical and populist political and philosophical traditions and the cyclical rather than novel nature of the popularity they currently enjoy. Empirically, MYPLACE employs a combination of survey, interview and ethnographic research instruments to provide new, pan- European data that not only measure levels of participation but capture the meanings young people attach to it. Analytically, through its specific focus on youth and the historical and cultural contextualization of young peoples social participation, MYPLACE replaces the routine, and often abstract, iteration of the reasons for young peoples disengagement from politics with an empirically rich mapping of young peoples understandings of the civic and political space that they inhabit. In policy terms, MYPLACE identifies the obstacles to, and facilitators of, young peoples reclamation of the European political arena as a place for them.

Agency: Cordis | Branch: FP7 | Program: CP-FP | Phase: SSH-2007-5.2-01;SSH-2007-3.3-01 | Award Amount: 1.72M | Year: 2008

The proposed research project is aimed at an in-depth understanding of the ways in which the modern European identities and regional cultures are formed and inter-communicated in the Eastern part of the European continent. The project is an inter-disciplinary effort (sociology, political science, history, ethnography). Its methodological thrust is empirical and comparative; new data complementing prioritiess will be generated. On top of that, the project pursues the objective to verify and advance existing theoretical and methodological frameworks for ethnic studies as well studies of identities and nationalism. In order to account for the diversity encountered in the field and to adequately identify the main factors in the identity formation process, ethnic groups, which are part of larger titular nations and are divided by the new European frontier toward the New Independent States are studied. Altogether, 8-10 million people have been affected by politically set boundaries. The project clusters based on major themes such as Formation and interplay of identities and ethnic cultures in Europe, Nations between states, Self-governance, representation and citizenship, Historical memory and dynamic trajectories of the development of ethnic minorities in Eastern Europe. Project assumes a detailed study of a sample of 10 ethnic minorities in EE by the means of a series of quantitative and qualitative surveys focusing on the identities and values issues. Four regional workshops and a final conference will conclude the project.

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