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Amir M.K.,Quaid-i-Azam University | Khan S.Z.,Quaid-i-Azam University | Khan S.Z.,University of Science & Technology Bannu | Hayat F.,Quaid-i-Azam University | And 3 more authors.
Inorganica Chimica Acta

Although it is well known that cisplatin and its analogues are effective anticancer agents, but their clinical use is restricted by some serious side effects. Palladium complexes are emerged as alternative metallo-anticancer drugs merited by their structural similarity to platinum(II) complexes, more labile nature, minimal chemoresistance and often water solubility. However, due to exceptional high reactivity of palladium complexes than their platinum counterparts, they are not only obstructed by the sulfur containing molecules to reach their pharmacological targets but also significantly enhance their affinity to convert into inactive trans isomers. This hitch can be overcome by the use of appropriate ligands that can have the potential to turn down the negative lability to positive inertness. This review provides a summary of the anticancer potential, DNA-binding and DNA-denaturing aptitude of various palladium(II) dithiocarbamates in which the presence of the dithiocarbamate moiety significantly improves the anticancer action and, at the same time, reduces the possibility of any damaging side effects. © 2016 Elsevier B.V. Source

Khan S.Z.,Quaid-i-Azam University | Khan S.Z.,University of Science & Technology Bannu | Amir M.K.,Quaid-i-Azam University | Naseer M.M.,Quaid-i-Azam University | And 4 more authors.
Journal of Coordination Chemistry

Two new heteroleptic Pd(II) dithiocarbamates (1 and 2) have been synthesized by reaction of equimolar quantities of palladium(II) chloride, sodium 4-(3-methoxyphenyl)piperazine-1-carbodithioate, and appropriate substituted triphenylphosphines. The synthesized complexes have been characterized by their physical, spectral (IR, 1H, 13C, and 31P NMR), and X-ray crystallographic data. Complexes 1 and 2 showed square-planar geometry both in solution and solid states. The crystal packing of both complexes revealed similar 3-D-supramolecular networks comprised of 1-D chains. However, the nature and strength of various non-covalent interactions of these networks were slightly different. The DNA interaction studies of the complexes have been carried out by UV–visible spectroscopy to evaluate their anticancer potential. The study suggested intercalative interaction with 2.402 × 104 and 2.713 × 103 M−1 binding constants, respectively. The complexes have also been evaluated for their anticancer activity against HeLa cell line. Both complexes showed higher activity with IC50 values much lower (22.176 and 26.166 μM for 1 and 2, respectively) than the standard cisplatin (78.075 μM). Furthermore, the complexes induced stronger DNA fragmentation as investigated by DNA ladder assay for apoptosis. Our findings suggested that the anticancer action of these compounds stems from their interaction with DNA leading to DNA damage and apoptosis. The excellent activity of 1 and 2 deserves to be a focus for further research and in vivo studies. © 2015 Taylor & Francis Source

Shafique S.,Quaid-i-Azam University | Khan M.R.,Quaid-i-Azam University | Nisar M.,University of Science & Technology Bannu | Shafiqure-Ur-Rehman,Jammu University
Pakistan Journal of Botany

In the present investigation 34 Pakistani Black gram cultivars were evaluated through morphological traits, SDS-PAGE analysis and random amplified polymorphic markers (RAPD) which revealed considerable amount of genetic diversity in this species. Among the morphological traits, dry pod weight (71.79%), number of branches per plant (51.16%) and biological yield (50.12%) showed highest level of coefficient of variation. Percent frequency distribution also showed highest level of genetic diversity. Positive significant correlation was observed between numbers of pods per plant and dry pod length (p=0.01); grain yield and number of seeds per plant (p=0.02), and 100 seeds weight and biological yield (p=0.04). A total of 20 cultivars were identified and characterized on the basis of maximum and minimum value for 10 quantitative traits. The combinations of 20 cultivars are having practical application for breeding program as well as for direct selection of super cultivar. Among the biochemical and molecular makers, RAPD marker showed high level genetic similarity difference (0.00-4.00) as compared to SDS-PAGE (0.00-2.45). RAPD analysis is considered the best option for determining Black gram genetic diversity pattern and Gene Bank management. Source

Manan A.,University of Science & Technology Bannu | Ullah I.,University of Science & Technology Bannu | Ahmad A.S.,Islamia College | Ullah A.,Wuhan University of Technology
Journal of Materials Science: Materials in Electronics

Ca1-xBaxLa4Ti5O17 (0 ≤ x ≤ 0.2) compositions were fabricated via a solid-state mixed oxide route and their phase, microstructure and microwave dielectric properties were investigated. All the compositions formed single phase in the Ca1-xBaxLa4Ti5O17 (0 ≤ x ≤ 0.2) solid solutions within the detection limit of in-house X-ray diffraction. The sintered microstructure of these ceramics comprised densely packed elongated and plate-like grains. The dielectric properties varied linearly with increasing x content. Relative permittivity (εr) increased from 53.2 to 56.6, Qufo decreased from 14,600 to 12,630 GHz and τf was tuned through zero and increased from -33.6 to 1.4 ppm/ºC with increase in x value from 0 to 0.2. In the present study, εr≈ 56.6, Qufo≈ 12,630 GHz and a near zero τf ≈ 1.4 ppm/ºC was achieved for Ba0.2Ca0.80La4Ti5O17(x = 0.2) ceramics. © Springer Science+Business Media New York 2016. Source

Ahmed W.,University of Science & Technology Bannu | Ahmed W.,University of Science & Technology Bannu | Ahmad M.,University of Science & Technology Bannu | Ahmad M.,University of Science & Technology Bannu | And 3 more authors.
Indian Journal of Animal Research

Snakebites are considered a neglected tropical disease that affects thousands of people worldwide. The available anti-venoms are associated with numerous side effects. As an alternative, researchers have extensively studied the plants in order to obtain some unusual treatment. The current study was conceded to evaluate the effects of salix nigra (Black willow) against krait snake venom acetylcholinesterase and its role as anti-cancer, anti-oxidant agent. Standard protocols of Ellman, Meyer, Gyamfi and Ruch were used for enzyme inhibition, anti-cancer and free radicals scavenging assays respectively. The methanolic extract of Salix nigraproved to possess neutralization properties against krait snake (Bungarus Sindanus) venom acetylcholinesterase. Statistical data of the results showed that Salix nigra extract inhibited the krait venom acetylcholinesterase through concentration dependent manner. Kinetic analysis using Lineweaver Burk plot revealed that Salix nigra caused competitive type of inhibition i.e. km value increased while Vmax remain constant with an increase of extract's concentration. The calculated IC50 value of Salix nigra was found to be 177μg/ml. In DPPH free radical scavenging assay, extract showed good scavenging potential with IC50 value of 317μg/ml. Similarly, using H2O2 free radicals, the % scavenging of the extract at 100, 250, 500 and 1000μg/ml was 41, 54, 69 and 74% in comparison with ascorbic acid. Moreover, the extract exhibited good cytotoxic activity against brine shrimps in a dose dependent manner. The present work suggests, for the first time, that Salix nigra extract can be used not only as a potent inhibitor of snake venom acetylcholinesterase but also for the eradication of free radicals along with significant anticancer activity. © 2016, Agricultural Research Communication Centre. All rights reserved. Source

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