General Luna, Philippines

University of San Agustin
General Luna, Philippines

The University of San Agustin is a private Catholic university in Iloilo City, Philippines. With 40 initial students, it was established in 1904 as a preparatory school for boys by the Spanish Catholic missionaries under the oldest religious Roman Catholic order in the Philippines during the American colonial period, the Order of Saint Augustine . In 1917, it was incorporated and became Colegio de San Agustin de Iloilo. In March 1953, San Agustin attained university status making it the First University in Western Visayas. San Agustin is the first and only Augustinian university in the Asia-Pacific region.University of San Agustin now provides programs from Basic Education up to post graduate studies in the areas of Business, Education, Computer Studies, Arts and science, Performing Arts, Music, Engineering, Medical Technology, Nursing and Pharmacy. In 2013, the university celebrated its 60th year as an institution of higher learning. Wikipedia.

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Valeriano-Zapana J.A.,University of Campinas | Valeriano-Zapana J.A.,University of San Agustin | Segovia-Cruz F.S.,University of San Agustin | Rojas-Hualpa J.M.,University of San Agustin | And 3 more authors.
Toxicon | Year: 2012

A new serine protease with thrombin-like activity (TLBan) from Bothrops andianus (Andean Lancehead) was isolated in two chromatographic steps in LC molecular exclusion and reverse phase-HPLC. TLBan is a glycoprotein that contains both N-linked carbohydrates and sialic acid in its structure, with Mr ∼29kDa under reducing conditions and non-reducing ∼25kDa conditions and confirmed by MALDI-TOF mass spectrometry (25,835.65Da) and exhibited high specificity for BAρNA, Michaelis-Menten behavior with Km 5.4×10-1 M and the Vmax 7.9×10-1 nmoles ρ-NA/L/min for this substrate and high stability when was analyzed at different temperatures (25 to 60°C), pHs (4.0 to 8.0), was inhibited by soybean trypsin inhibitor, EDTA and phenylmethylsulfonyl fluoride (PMSF).The total amino acid sequence was obtained through sequencing of selected tryptic peptides and by inference obtained using SwissProt database with the search restricted to serine proteases from Crotalinae snakes and show high amino acid sequence identity with other serine proteases from snake venom. TLBan showed the presence of His(44), Asp(91) residues and Ser was deduced (187) position, in the corresponding positions to the catalytic triad established in the serine proteases and Ser(187) are inhibited by phenylmethylsulfonyl fluoride (PMSF).In this work, we investigated the ability of TLBan to degrade fibrinogen and we observed that it is able to cause α- and β-chain cleavage. Enzymatic activities as well as the platelet aggregation were strongly inhibited when were incubated with PMSF, a specific inhibitor of serine protease. TLBan showed a potential medical-scientific interest to understand the pathophysiological mechanism of the snake venom action and identification of new blood coagulation cascade acting enzymes of natural sources. © 2011 Elsevier Ltd.

Ortiz M.I.,Autonomous University of the State of Hidalgo | Ortiz M.I.,University of San Agustin | Fernandez-Martinez E.,Autonomous University of the State of Hidalgo | Soria-Jasso L.E.,Autonomous University of the State of Hidalgo | And 5 more authors.
Biomedicine and Pharmacotherapy | Year: 2016

Chamomile (Matricaria chamomilla L., Asteraceae) is a medicinal plant widely used as remedy for pain and gastric disorders. The association of non-steroidal anti-inflammatory drugs (NSAIDs) with medicinal plant extracts may increase its antinociceptive activity, permit the use of lower doses and limit side effects. The aim was to isolate and identify the main chemical constituents of Matricaria chamomilla ethanolic extract (MCE) as well as to explore their activity as cyclooxygenase (COX) inhibitors in silico; besides, to examine the interaction between MCE and diclofenac on nociception in the formalin test by isobolographic analysis, and to determine the level of gastric injury in rats. Three terpenoids, α-bisabolol, bisabolol oxide A, and guaiazulene, were isolated and identified by 1H NMR. Docking simulation predicted COX inhibitory activity for those terpenoids. Diclofenac, MCE, or their combinations produced an antinociceptive effect. The sole administration of diclofenac and the highest combined dose diclofenac-MCE produced significant a gastric damage, but that effect was not seen with MCE alone. An isobologram was constructed and the derived theoretical ED35 for the antinociceptive effect was significantly different from the experimental ED35; hence, the interaction between diclofenac and MCE that mediates the antinociceptive effect is synergist. The MCE contains three major terpenoids with plausible COX inhibitory activity in silico, but α-bisabolol showed the highest affinity. Data suggest that the diclofenac-MCE combination can interact at the systemic level in a synergic manner and may have therapeutic advantages for the clinical treatment of inflammatory pain. © 2016 Elsevier Masson SAS.

Perez C.,University of San Agustin | Klaustermeier J.E.,Catalan Institute of Nanoscience and Nanotechnology | Alemany L.,Hospitalet of Llobregat | Tous S.,Hospitalet of Llobregat | And 3 more authors.
Diagnostic Molecular Pathology | Year: 2012

The great interest in molecular epidemiology of human papilloma virus (HPV) in cervical cancer led us to perform a thorough evaluation of 2 polymerase chain reaction (PCR)-based methods for the detection of HPV in archival formalin-fixed paraffin-embedded (FFPE) samples. Thus, the aim of this study was to compare HPV detection in FFPE samples that have histopathologic diagnosis of invasive cervical cancer using SPF10 broad-spectrum primers PCR followed by DNA enzyme immunoassay and LiPA25 (version 1: Labo Biomedical products, Rijswijk, The Netherlands version 1) and the Papillomavirus Clinical Arrays technique (Genómica, Tres Cantos, Madrid, Spain). In this study, 235 biopsies with histopathologic diagnosis of invasive cervical cancer were analyzed for the detection and genotyping of HPV by LiPA25 SPF10-PCR System (version 1) and Papillomavirus Clinical Arrays technique. The detection of HPV DNA with Genómica technique was 75.1%, and 91.9% with LiPA25 SPF10-PCR. The Genómica technique detected a higher percentage of multiple infections (35%) than LiPA25 (8.9%), with a very low agreement for the detection of multiple infections between them (P>0.05). Our study highlights an important difference between 2 PCR-based methods for detection and genotyping of HPV. LiPA25 SPF10-PCR technology may be more adequate than Genómica for the detection of HPV DNA when using FFPE tissue. © 2012 by Lippincott Williams & Wilkins.

Prieto I.,CSIC - Estación Experimental De Zonas Áridas | Martinez-Tilleria K.,University of La Serena | Martinez-Manchego L.,University of San Agustin | Montecinos S.,University of La Serena | And 2 more authors.
Oecologia | Year: 2010

Hydraulic lift (HL) is the passive movement of water through the roots from deep wet to dry shallow soil layers when stomata are closed. HL has been shown in different ecosystems and species, and it depends on plant physiology and soil properties. In this study we explored HL patterns in several arid land shrubs, and developed a simple model to simulate the temporal evolution and magnitude of HL during a soil drying cycle under relatively stable climatic conditions. This model was then used to evaluate the influence of soil texture on the quantity of water lifted by shrubs in different soil types. We conducted transpiration suppression experiments during spring 2005 in Chile and spring 2008 in Spain on five shrub species that performed HL, Flourensia thurifera, Senna cumingii and Pleocarphus revolutus (Chile), Retama sphaerocarpa and Artemisia barrelieri (Spain). Shrubs were covered with a black, opaque plastic fabric for a period of 48-72 h, and soil water potential was recorded at different depths under the shrubs. While the shrubs remained covered, water potential continuously increased in shallow soil layers until the cover was removed. The model output indicated that the amount of water lifted by shrubs is heavily dependent on soil texture, as shrubs growing in loamy soils redistributed up to 3.6 times more water than shrubs growing on sandy soils. This could be an important consideration for species growing in soils with different textures, as their ability to perform HL would be context dependent. © Springer-Verlag 2010.

Tobias J.A.,University of Oxford | Gamarra-Toledo V.,University of Los Andes, Colombia | Gamarra-Toledo V.,University of San Agustin | Pulgarin P.C.,University of Antioquia | Seddon N.,University of Oxford
Journal of Evolutionary Biology | Year: 2011

The evolution of sexually monomorphic (i.e. mutual) ornamentation has attracted growing attention as a 'blind-spot' in evolutionary biology. The popular consensus is that female ornaments are subject to the same modes of sexual selection as males: intrasexual competition and mate choice. However, it remains unclear how these forces interact within and between sexes, or whether they fully capture selection on female traits. One possibility is that the 'armament-ornament' model - which proposes that traits used primarily in male-male contests are also co-opted by females as indicators of male quality - can be extended to explain signal evolution in both sexes. We examine this idea by testing the function of acoustic signals in two species of duetting antbirds. Behavioural observations and playback experiments suggest that male and female songs function primarily as armaments in competitive interactions. Removal experiments reveal that song is also a classic ornament used by unpaired males and females to advertise for mates. These results indicate that 'armament-ornament' processes may operate in reciprocal format, potentially explaining widespread mutual ornamentation in species with elevated intrasexual competition for resources. In addition, given that songs mediate competition between species outside the breeding season, our findings suggest that processes shaping monomorphic ornaments extend beyond the traditional definitions of sexual selection and are best understood in the broader framework of social selection. © 2011 The Authors. Journal of Evolutionary Biology © 2011 European Society For Evolutionary Biology.

Florez O.U.,Utah State University | Ocsa A.,University of San Agustin | Dyreson C.,Utah State University
MIR 2010 - Proceedings of the 2010 ACM SIGMM International Conference on Multimedia Information Retrieval | Year: 2010

This paper introduces a novel hashing algorithm for large timeseries databases, which can improve the querying of human motion. Timeseries that represent human motion come from many sources, in particular, videos and motion capture systems. Motion-related timeseries have features which are not commonly present in traditional types of vector data and that create additional indexing challenges: high and variable dimensionality, no Euclidean distance without normalization, and a metric space not fully defined. New techniques are needed to index motion-related timeseries. The algorithm that we present in this paper generalizes the dot product operator to hash timeseries of variable dimensionality without assuming constant dimensionality or requiring dimensionality normalization, unlike other approaches. By avoiding normalization, our hashing algorithm preserves more timeseries information and improves retrieval accuracy, and by hashing achieves sublinear computation time for most searches. Additionally, we show how to further improve the hashing by partitioning the search space using timeseries within the index. This paper also reports the results of experiments that show that the algorithm performs well in the querying of real human motion datasets. Copyright 2010 ACM.

Ortiz M.I.,Autonomous University of the State of Hidalgo | Ortiz M.I.,University of San Agustin
Pain Research and Management | Year: 2013

BACKGROUND: There is evidence that biguanide3 and sulfonylurea: block diclofenac-induced antinociception (DIA) in rat models. However, little i3 known about tke interaction between these hypoglycemics with respect to DIA. OBJECTIVE: To determine whether metformin-sulfonylurea combinations affect DIA during the formalin te3t. METHODS: Rats received the appropriate vehicle or diclofenac before 1% formaldehyde was injected into the paw. Rats were also pretreated with vehicle, glibenclamide, glipizide, metformin or glibenclamide/metformin and glipizide/metformin combinations before the diclofenac and formaldehyde injections, and the effect on antinociception was assessed. Isobolograms of the combinations were constructed to test for a synergistic interaction. RESULTS: Systemic injection of diclofenac resulted in antinociception during the second phase of the test. Systemic pretreatment with the combinations of glibenclamide (0.56 mg/kg to 10 mg/kg )/metform in (10 mg/kg to ISO mg/kg) and glipizide (0.56 mg/kg to 10 mg/kg)/metformin (10 mg/kg to ISO mg/kg) blocked DIA. The derived theoretical effective doses for 50% of subjects (ED50) for the glibenclamide/metformin and glipizide/ metformin combinations were 32.52 mg/kg and 32.42 mg/kg, respectively, and were significantly higher than the actual observed experimental ED50 values (7.57 mg/kg and 8.43 mg/kg, respectively). CONCLUSION: Pretreatment with glibenclamide, glipizide or met-formin blocked DIA in a do3e-dependent manner, and combining either sulfonylurea with metformin produced even greater effects. The observed ED50S for the combinations were approximately fourfold lower than the calculated additive effects. These data indicate that sulfonylureas interact to produce antagonism of DIA. Combination therapy is a common second- line treatment for patients with diabetes and metabolic syndrome, a group that experiences pain from multiple sources. The results suggest that at least some anti-inflammatory agent3 may not be effective in this group. © 2013 Pulsus Group Inc.

Castro-Rodriguez J.A.,University of Santiago de Chile | Molina R.O.,University of San Agustin | Caceres M.,University of San Agustin | Recabarren A.,University of San Agustin | Recabarren A.,Hospital III Yanahuara EsSalud
Pediatric Pulmonology | Year: 2014

Objective: To determine if nitrites (nitric oxide metabolites) measured in induced sputum decrease and correlate with improvement of clinical asthma symptoms after treatment, we performed a prospective longitudinal study in a tertiary care hospital in Arequipa, Peru. Methods: In 95 schoolchildren with mild and moderate persistent asthma we determined nitrites in induced sputum samples (measured using the Griess assay). Clinical parameters and exercise bronchial challenge (EBC) test were performed twice, at baseline and after 3 months of beclomethasone-dipropionate treatment (median doses: 300 mcg/day, IQR: 300-450). Results: Sixty out of 95 children completed the study (median age of 9-year [IQR: 7-13]). A significant change in sputum nitrites levels between admission and the end of the study was observed (34.4 nmol/ml [IQR:18.2-58.4] and 11.2 nmol/ml [6-20.1], respectively, P = < 0.0001). Also a significant correlation between decrease of sputum nitrites levels and improvement of clinical parameters (acute exacerbations [r = 0.361, P = 0.005]; use of salbutamol [r = 0.322, P = 0.013]; emergency visits [r = 0.275, P = 0.033]; and school absence [r = 0.41, P = 0.001]) from admission to the end of the study was found. However, sputum nitrites levels did not correlated with peripherical blood eosinophils or serum IgE levels or with EBC test at any point of the study. The decrease of sputum nitrites levels after the treatment was significant in each asthma group (mild and moderate), but not between groups. Conclusion: This study showed that measured nitrite in induced sputum (a simple and cheap non-invasive method) is a good alternative for monitoring asthmatic treatment in schoolchildren. © 2013 Wiley Periodicals, Inc.

Objective: To evaluate the vasodilator response of the hydroalcoholic extract of Zea mays L. (Andean purple corn) and to determine if this response is mediated by nitric oxide (NO). Material and methods: We obtained an extract by maceration for eight days of Andean purple corn cobs in 70% ethanol and subsequent concentration of the product. Thoracic aortic rings were evaluated in an isolated organ chamber, bathed with Krebs-Hensleit solution (KH), and vasomotor activity was recorded with an isometric tension transducer. Basal contraction was produced with 120 mM KCl and then, we proceeded to determinate the vasodilator effect of 3 doses of the extract: 0.1, 0.5, and 1.0 mg/mL. We used L-NG-Nitroarginin methyl ester (L-NAME) to verify that the vasodilation depends on nitric oxide sinteasa (NOs). Then we compared the inhibition of vascular contraction after incubation for 30 minutes, with purple corn extract and captopril 10-5 M. Results: We observed a reduction in maximum contraction (100%) to 85.25 ± 2.60%, 77.76 ± 3.23%, and 73.3 ± 4.87% for doses of 0.1, 0.5 and 1,0 mg/mL respectively. The vasodilation was inhibited by prior incubation with L-NAME. Andean purple corn extract did not inhibit vascular contraction as captopril did (reduction to 75.27 ± 8.61%). Conclusion. The hydroalcoholic extract of Zea mays L produces NO dependent vasodilation.

Cuba R.F.B.,University of San Agustin
Proceedings of the 6th IASTED International Conference on Software Engineering and Applications, SEA 2002 | Year: 2012

The problem with complex games is that there are many ways to play them. In other words the group of possible strategies is large. However, if we consider a strategy as a succession of pure strategies through generations in the game (as a game tree), we can solve the problem with a Genetic Algorithm (GA). Furthermore, fitness function which evaluates the individuals needs to know the state of the game, in order to apply the best strategy. Representing discreetly the state of the game (pattern) we can train a Backpropagation Net (BPN) to recognize it. This research work proposes a method to solve games with perfect information from Artificial Intelligence's perspective. The use of this method will be shown on the development of a hybrid system, which can play a Chess departure successfully. The advantage between this method and the extensive method is that the number of individuals in each generation is always the same (only the best strategies survive).

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