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Castro M.D.R.,University of Puerto Rico at San Juan | Suarez E.,Ponce School of Medicine & Health Sciences | Suarez E.,University of Puerto Rico-Ponce Campus | Kraiselburd E.,University of Puerto Rico at San Juan | And 4 more authors.
Experimental Gerontology | Year: 2012

While the mechanisms of cellular aging remain controversial, a leading hypothesis is that mitochondrial oxidative stress and mitochondrial dysfunction play a critical role in this process. Here, we provide data in aging rhesus macaques supporting the hypothesis that increased oxidative stress is a major characteristic of aging and may be responsible for the age-associated increase in mitochondrial dysfunction. We measured mitochondrial DNA (mtDNA) damage by quantitative PCR in liver and peripheral blood mononuclear cells of young, middle age, and old monkeys and show that older monkeys have increases in the number of mtDNA lesions. There was a direct correlation between the amount of mtDNA lesions and age, supporting the role of mtDNA damage in the process of aging. Liver from older monkeys showed significant increases in lipid peroxidation, protein carbonylations and reduced antioxidant enzyme activity. Similarly, peripheral blood mononuclear cells from the middle age group showed increased levels in carbonylated proteins, indicative of high levels of oxidative stress. Together, these results suggest that the aging process is associated with defective mitochondria, where increased production of reactive oxygen species results in extensive damage at the mtDNA and protein levels. This study provides valuable data based on the rhesus macaque model further validating age-related mitochondrial functional decline with increasing age and suggesting that mtDNA damage might be a good biomarker of aging. © 2011 Elsevier Inc.


Alicea-Serrano A.M.,University of Puerto Rico-Ponce Campus | Contreras M.,Venezuelan Institute for Scientific Research | Magris M.,Amazonic Center for the Research and Control of Tropical Diseases | Hidalgo G.,Amazonic Center for the Research and Control of Tropical Diseases | And 2 more authors.
Archives of Microbiology | Year: 2013

Newborns acquire their first microbiota at birth. Maternal vaginal or skin bacteria colonize newborns delivered vaginally or by C-section, respectively (Dominguez-Bello et al. 2010 #884). We aimed to determine differences in the presence of four tetracycline (tet) resistance genes, in the microbes of ten newborns and in the mouth and vagina of their mothers, at the time of birth. DNA was amplified by PCR with primers specific for [tet(M), tet(O), tet(Q), and tet(W)]. Maternal vaginas harbored all four tet resistance genes, but most commonly tet(M) and tet(O) (63 and 38 %, respectively). Genes coding for tet resistance differed by birth mode, with 50 % of vaginally delivered babies had tet(M) and tet(O) and 16 and 13 % of infants born by C-section had tet(O) and tet(W), respectively. Newborns acquire antibiotic resistance genes at birth, and the resistance gene profile varies by mode of delivery. © 2013 Springer-Verlag Berlin Heidelberg.


Hernandez-Becerril D.U.,National Autonomous University of Mexico | Nelson N.R.,University of Puerto Rico-Ponce Campus | Baron-Campis S.A.,Instituto Nacional Of Pesca | Moreno-Gufierrez S.P.,National Autonomous University of Mexico
Cryptogamie, Algologie | Year: 2013

In this paper, we show morphological features of two marine planktonic diatoms that are phylogenetically related, as suggested by molecular studies: Bellerochea malleus and Helicotheca tamesis. In order to study Bellerochea malleus specimens of the species were isolated from samples collected from marginal coral reefs located in the southern portion of Puerto Rico and cultured in the laboratory. Specimens of the species Helicotheca tamesis were isolated from net phytoplankton samples collected from the tropical Mexican Pacific and also cultured in the laboratory. The two species were examined by means of light and scanning electron microscopy. The morphology of Bellerochea malleus agrees well with previous reports: cells arranged in relatively long chains, united by the horn-shaped apical elevations, elliptical valves (not triangular) with depressed valve face bearing an eccentric annulus, from which numerous costae radiate, formation of costate ocelli, and one eccentric or marginal protruded and tubular rimoportula per valve. Helicotheca tamesis also shows the typical morphological features of this species: cells are solitary or form long and distinctly twisted chains, with no apparent apertures between sibling valves, valves are linear to elliptical, weakly silicified and flat. Furthermore, we found one conspicuous and protruded rimoportula per valve, located eccentrically or marginally (not central as previously described), consisting of an elliptic tubular structure. The cingulum is composed of numerous half-bands and segmented copulae. This study compares the morphology of these two species, with a special emphasis on the structure of the rimoportulae. © 2013 Adac. Tous droits réservés.


de Jesus-Laboy K.M.,University of Puerto Rico-Ponce Campus | Godoy-Vitorino F.,University of Puerto Rico-Ponce Campus | Godoy-Vitorino F.,U.S. Department of Energy | Piceno Y.M.,Lawrence Berkeley National Laboratory | And 5 more authors.
Genes | Year: 2012

Animals have co-evolved with mutualistic microbial communities, known as the microbiota, which are essential for organ development and function. We hypothesize that modern animal husbandry practices exert an impact on the intestinal microbiota. In this study, we compared the structure of the fecal microbiota between feral and domestic goats using the G2 PhyloChip and assessed the presence of five tetracycline resistance genes [tet(M), tet(S), tet(O), tet(Q) and tet(W)] by PCR. Feces were collected from 10 goats: 5 domestic from a farm in the main island of Puerto Rico and 5 feral from the remote dry island of Mona. There were 42 bacterial phyla from 153 families detected in the goats' feces. A total of 84 PhyloChip-OTUs were different in the fecal microbiota of feral and domestic goat. Both feral and domestic goats carried antibiotic resistance genes tet(O) and tet(W), but domestic goats additionally carried tet(Q). Diet, host genetics and antibiotic exposure are likely determinant factors in shaping the intestinal microbiota and may explain the differences observed between feral and domestic goats fecal microbiota. © 2012 by the authors; licensee MDPI, Basel, Switzerland.


Aleno R.,University of Puerto Rico-Ponce Campus | Collazo A.L.,University of Puerto Rico-Ponce Campus | Medina E.,University of Puerto Rico-Ponce Campus | Figueroa L.D.,University of Puerto Rico-Ponce Campus | And 2 more authors.
Materials Research Society Symposium Proceedings | Year: 2014

Due to the rapid advance of the emergence of resistant microorganisms to different antibiotics, there is a need to create new antimicrobial agents. It is possible that Nanotechnology has a great impact in this area since the nanoparticles can improve the antimicrobial effect of the antibiotics. In this study we used three different metal oxides nanoparticles, the MgO, ZnO and CuO. These nanoparticles were selected because their interactions leading to cell death and their optical properties. The aim of this study is to develop new methods that are more effective against resistance bacteria, developing antibacterial agents using different nanoparticles against Escherichia coli (ATCC 10536), Pseudomonas aeruginosa (ATCC 10145), and Staphylococcus aureus (ATCC BAA-1026). This study was conducted to evaluate the antibacterial effects of a combination of nanoparticles together with different concentrations of three antibiotics, Gentamicin, Cephalexin and Co-Trimoxazole. The results showed that some nanoparticles are effective to inhibit growth in these microorganisms by increasing the effectiveness of the antibiotic. Therefore, the present study indicates that the combination of the nanoparticles with antibiotics may be applicable as a new antimicrobial agent. © 2014 Materials Research Society.


PubMed | University Institute of Health Sciences and University of Puerto Rico-Ponce Campus
Type: Journal Article | Journal: Journal of clinical & cellular immunology | Year: 2016

Inflammatory bowel diseases (IBD) are chronic relapsing inflammatory conditions of unknown cause and likely result from the loss of immunological tolerance, which leads to over-activation of the gut immune system. Gut macrophages and dendritic cells (DCs) are essential for maintaining tolerance, but can also contribute to the inflammatory response in conditions such as IBD. Current therapies for IBD are limited by high costs and unwanted toxicities and side effects. The possibility of reducing intestinal inflammation with DCs genetically engineered to over-express the apoptosis-inducing FasL (FasL-DCs) has not yet been explored.Investigate the immunomodulatory effect of administering FasL-DCs in the rat trinitrobenzene sulfonic acid (TNBS) model of acute colitis.Expression of FasL on DCs isolated from the mesenteric lymph nodes (MLNs) of normal and TNBS-colitis rats was determined by flow cytometry. Primary rat bone marrow DCs were transfected with rat FasL plasmid (FasL-DCs) or empty vector (EV-DCs). The effect of these DCs on T cell IFN secretion and apoptosis was determined by ELISPOT and flow cytometry for Annexin V, respectively. Rats received FasL-DCs or EV-DCs intraperitoneally 96 and 48 hours prior to colitis induction with TNBS. Colonic T cell and neutrophil infiltration was determined by immunohistochemistry for CD3 and myeloperoxidase activity assay, respectively. Macrophage number and phenotype was measured by double immunofluorescence for CD68 and inducible Nitric Oxide Synthase.MLN dendritic cells from normal rats expressed more FasL than those from colitic rats. Compared to EV-DCs, FasL-DCs reduced T cell IFN secretion and increased T cell apoptosis FasL-DCs are effective at treating colonic inflammation in this model of IBD and represent a possible new treatment for patients with IBD.


University of Puerto Rico-Ponce Campus | Entity website

Revista Huellas del Futuro | 2016 Volmen III Animated publication To maximize your viewing experience of this digital catalog, we recommend installing Adobe Flash Player Plugin. This installation will only take a few moments ...


University of Puerto Rico-Ponce Campus | Entity website

La Constitucin Apostlica del Sumo Pontfice Juan Pablo II, Ex corde Ecclesiae, define la Pastoral Universitaria como: Aquella actividad de la Universidad que ofrece a los miembros de la Comunidad la ocasin de coordinar el estudio acadmico y las actividades para-acadmicas con los principios religiosos y morales integrando de esta manera la vida con la fe. (Ex corde Ecclesiae, 38) El mismo documento seala que, Dicha pastoral concretiza la misin de la Iglesia en la Universidad y forma pare integrante de su actividad y de su estructura: (38) Por lo tanto, es de suma importancia que, Como natural expresin de su identidad catlica, la comunidad universitaria deba saber encarnar la fe en sus actividades diarias (39) Adems, Segn su propia naturaleza, toda Universidad Catlica presta una importante ayuda a la Iglesia en su misin evangelizadora (49) CapellanaLos Capellanes Sern responsables de ofrecer a los miembros de la comunidad universitaria los servicios de capellana inherentes a la labor pastoral que le corresponde a la Universidad como institucin catlica ...


University of Puerto Rico-Ponce Campus | Entity website


University of Puerto Rico-Ponce Campus | Entity website

La Oficina de Asistencia Econmica de la Pontificia Universidad Catlica de Puerto Rico te provee, si cualificas, la ayuda econmica que necesitas para alcanzar tus metas acadmicas. Este proceso comienza cuando radicas la solicitud de FAFSA (Solicitud de Ayuda Gratuita Federal) por cada ao acadmico por el cual desees recibir ayudas econmicas ...

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