Ramon Castilla, Peru

National University of Piura

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Ramon Castilla, Peru
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Tume Farfan L.F.,National University of Piura | Tume Farfan L.F.,Laboratorio Of Biologia Molecular
Inmunologia | Year: 2014

There is evidence that supports the hypothesis of the existence of cancer stem cells (CSC), in which it postulates that these are responsible for the initiation, recurrence, metástasis, and resistance to cancer treatments, thus creating a need for therapies that specifically target these subpopulations of cells with stem cell characteristics in most malignant tumors with mixed cell phenotypes. Since its advent, immunotherapy has been used as an attractive approach due to the many shortcomings of conventional surgery, radiotherapy and chemotherapy in the treatment of cancer. Within tumor subsets neoplastically transformed cells demonstrate surface expression of molecules that are not typically present on the surface of the surrounding normal cells. In some cases, especially in malignant melanoma, the cytotoxic T lymphocytes (CTL) directed against tumor associated antigens (TAA) have been isolated to raise antibodies and somehow reduce the disease. The focus of cancer vaccine therapy is based on the idea that the immune system could mount a rejection response force against conglomerate neoplastically transformed cells. However, due to the low immunogenicity of TAA, down-regulation of MHC molecules, the lack of expression of appropriate co-stimulatory molecule, cytokine secretion inmunoinhibitorias etc., they rarely met expectations. This is why new markers that are not only safe and allow immunotherapy directed against CSC are now being sought, but also tumor eradication could be achieved with the combination of many therapies in order to have an efficient outcome in the treatment of this disease currently affecting the world. © 2014 Sociedad Espanola de Inmunologia.


PubMed | University of San Martín de Porres, National University of Piura, San Luis Gonzaga National University and Catholic University of Peru
Type: Journal Article | Journal: Le infezioni in medicina : rivista periodica di eziologia, epidemiologia, diagnostica, clinica e terapia delle patologie infettive | Year: 2016

Not available.


Mejia C.R.,Asociacion Medica de Investigacion y Servicios en Salud | Mejia C.R.,Ricardo Palma University | Quinones-Laveriano D.M.,Ricardo Palma University | Espinoza K.G.,Ricardo Palma University | Quezada-Osoria C.,National University of Piura
Revista Peruana de Medicina Experimental y Salud Publica | Year: 2013

The aim of this study was to describe the concentration of lead in umbilical cord blood (UCB) of neonates born from primiparous women who live in northern Lima. A cross sectional study was carried out at Cayetano Heredia National Hospital, from July 2011 to January 2012. 100 neonates born from primiparous women who lived in northern Lima in the previous 5 years or more were included. Umbilical cord lead levels were measured by the atomic adsorption method, and the demographic information and risk factors of the neonates were filled in a patient record designed to this purpose. 30% of the neonates had concentration of lead in UCB that was considered as increased risk (≥ 3,0 μg/dL), 16% of the cases had had toxic concentration of lead in UCB (≥ 5 μg/dL). We conclude that there are a high percentage of neonates contaminated with lead in the northern Lima.


Manchester S.R.,University of Florida | Herrera F.,University of Florida | Herrera F.,Smithsonian Tropical Research Institute | Fourtanier E.,California Academy of Sciences | And 2 more authors.
Journal of Geology | Year: 2012

The Belén flora, in north coastal Peru, is the most diverse fruit and seed assemblage yet known from the Paleogene of South America. Little original paleobotanical work has been performed on this assemblage since the pioneering treatments published by E. W. Berry in the 1920s, and the precise age has not been determined. Nevertheless, the flora has been regarded as a focal point in understanding the vegetational, orogenic, and climatic history of northern South America, and in recent literature it has been assumed to be early Eocene. In order to tighten this age assignment, which has varied from early Eocene to early Oligocene in the opinions of different authors, we revisited the Belén site, measured the stratigraphic section, and processed the fruit- and seed-containing sediment for age-diagnostic microfossils. Although pollen and foraminifera were not recovered, the sediment is rich in diatoms. The diatom assemblage includes Lisitzinia ornata and Rocella vigilans, among others, indicating a latest early Oligocene age (~30-28.5 Ma) for these deeper marine sediments, which we infer to have been subsequently reworked into the Belén environment. We also reevaluate the botanical identifications, which are based on the original museum specimens supplemented by more recently collected specimens. The Belén flora provides a window into extinct forests in South America that were present before the rising of the Andes in western Peru. © 2012 by The University of Chicago.


PubMed | National University of Piura, University of Washington and Antenor Orrego Private University
Type: Journal Article | Journal: American journal of medical genetics. Part A | Year: 2016

Congenital generalized lipodystrophy (CGL) is a genetically heterogeneous group of disorders characterized by the absence of functional adipose tissue. We identified two pedigrees with CGL in the community of the Mestizo tribe in the northern region of Peru. Five cases, ranging from 15 months to 7 years of age, presented with generalized lipodystrophy, muscular prominence, mild intellectual disability, and a striking aged appearance. Sequencing of the BSCL2 gene, known to be mutated in type 2 CGL (CGL2; Berardinelli-Seip syndrome), revealed a homozygous deletion of exon 3 in all five patients examined, suggesting the presence of a founder mutation. This intragenic deletion appeared to be mediated by recombination between Alu sequences in introns 2 and 3. CGL2 in this population is likely underdiagnosed and undertreated because of its geographical, socio-economic, and cultural isolation. 2016 Wiley Periodicals, Inc.


Rodriguez-Galvez E.,National University of Piura | Maldonado E.,National University of Piura | Alves A.,University of Aveiro
European Journal of Plant Pathology | Year: 2014

Production of table grapes is a major export commodity in the region of Piura, one of the most important grape-production areas of Peru. Characteristic symptoms of Botryosphaeria dieback were observed in vineyards throughout this region. The aim of this study was to characterise the symptoms of dieback of table grapes; evaluate the incidence and prevalence of the disease; identify the aetiological agent and evaluate its pathogenicity and virulence. The disease was present in all vineyards surveyed but incidence varied with location. A botryosphaeriaceous fungus was isolated from symptomatic plants collected in five different vineyards in Piura. On the basis of morphological and cultural characters as well as phylogenetic analysis of the internal transcribed spacer region (ITS), and part of the translation elongation factor 1-α gene (EF1-α) the isolates were identified as belonging to the species Lasiodiplodia theobromae. Inoculation of cv. Red Globe plants confirmed the pathogenicity of the isolates and revealed differences in virulence among the isolates tested. This is the first report of L. theobromae as causal agent of dieback of table grapes in Peru, which represents a threat to Peruvian vineyards productivity and longevity. © 2014, Koninklijke Nederlandse Planteziektenkundige Vereniging.


The Peruvian Plantcutter (Phytotoma raimondii) is an endemic bird from northwestern Peru. It is considered endangered due to habitat fragmentation. Although there are many studies on this species, some aspects of its natural history and habitat requirements remain little studied. Here, I assess home range, site fidelity, and resource selection in four pairs of Peruvian Plantcutters in the dry forest of Talara-Piura between February and August 2013. Home ranges varied from 0.68 to 3.44 ha (mean = 1.70 ± 0.99). There was strong evidence for site fidelity of all eight individuals. Plantcutters favored the use of palo negro (Grabowskia boerhaaviaefolia) and algarrobo (Prosopis pallida). These plants are used by the Peruvian Plantcutter for foraging, nesting, and shelter; therefore both plants constitute important resources, which must be considered in conservation plans for the species.


Tume Farfan L.F.,National University of Piura
Revista Venezolana de Oncologia | Year: 2014

The evidence supports the hypothesis of the cancer stem cells, which postulate that they are responsible for the initiation, the recurrence, metastasis and the resistance to anti neoplasia treatments, creating the need for therapies aimed specifically at these subpopulations of cells with characteristics of stem cells within the most heterogeneous malignant tumors. Within tumors subsets of the cells transformed in neoplasia show the expression on its surface of molecules that are not typically present on the surface of the surrounding normal cells. In some cases, especially in the malignant melanomas, the lymphocytes T cytotoxic directed against these antigens associated with tumors have been isolated to create antibodies and somehow reduce the disease. The approach to vaccine against cancer therapy is based on the idea that the immune system could saddle a rejection response force against the conglomerate of the cells transformed neoplasia. The new markers that are safe and allow direct immunotherapy for eradication of the tumor, with the combination of many therapies, and thus have an efficient outcome in the treatment of this disease is currently looking for.


Tume Farfan L.F.,National University of Piura | Tume Farfan L.F.,Laboratorio Of Biotecnologia Molecular
Revista Venezolana de Oncologia | Year: 2015

We know that our cells have gene expression patterns that can be on altered over and give rise lead to cancer. The epigenetic is the study of the changes in the gene expression without modifying the DNA sequence. The epigenetic and the genetic alterations are considered as two separate mechanisms involved in the onset and progression of the cancer. During the last decade have emphasized the field of the epigenetics to find applications in the diagnosis and therapy in this disease, along with microRNAs play an important role in the silencing of certain genes against certain conditions. In this review, we study and discussed the epigenetic changes and microRNAs that are notorious in the lung cancer and the importance of both in the early diagnosis of this disease and his therapy they are discussed. © 2015, Sociedad Venezolana de Oncologia. All rights reserved.


Tume-Farfan L.F.,National University of Piura
Gaceta Mexicana de Oncologia | Year: 2014

The cells acquire different patterns of gene expression during differentiation to adapt to a changing environment. Epigenetic and genetic alterations are considered to be 2 independent mechanisms involved in the onset and progression of cancer. Epigenetic mechanisms may be important for biological events such as the genetic mechanisms that do not involve a change in DNA sequence, but if they have an important role in modifying gene expression. During the last decade, research in epigenetic alterations in cancer progression has been improved thanks to the emergence of new technologies, to find applications in the diagnosis and therapy of this disease. In this review is discussed the current evidence on the role of epigenetic mechanisms in the progression of various cancers highlighting the advantages of the investigation of epigenetics in future new treatments. 1665-9201 © 2014 Gaceta Mexicana de Oncología. Publicado por Masson Doyma México S.A. Todos los derechos reservados.

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