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Gorzów Śląski, Poland

Slowinska-Lisowska M.,Wroclaw University | Szygula Z.,University of Physical Education in Warsaw | Witkowski Z.,Wroclaw University | Szyszka K.,University of Physical Education Poznan
Journal of Physiology and Biochemistry

The aim of this study was to follow up whether the modification of pro-antioxidant status by 8-day oral application of N-acetylcysteine (NAC) in healthy men affects the haematological response, whether there is a direct relationship between antioxidant defences and erythropoietin (EPO) secretion and whether NAC intake enhances exercise performance. Fifteen healthy men were randomly assigned to one of two groups: control or NAC (1,200 mg d-1 for 8 days prior to and 600 mg on the day of exercise trial). To measure the ergogenic effectiveness of NAC, subjects performed incremental cycle exercise until exhaustion. NAC administration significantly influenced the resting and post-exercise level of glutathione (+31%) as well as the resting activity of glutathione enzymes (glutathione reductase, -22%; glutathione peroxidase, -18%). The oxidative damage markers, i.e., protein carbonylation and lipid peroxidation products (thiobarbituric acid reactive substance) were reduced by NAC by more than 30%. NAC noticeably affected the plasma level of EPO (+26%), haemoglobin (+9%), haematocrit (+9%) and erythrocytes (-6%) at rest and after exercise. The mean corpuscular volume and the mean corpuscular haemoglobin increased by more than 12%. Plasma total thiols increased by 17% and directly correlated with EPO level (r= 0.528, P<0.05). NAC treatment, contrary to expectations, did not significantly affect exercise performance. Our study has shown that 8-day NAC intake at a daily dose of 1,200 mg favours a pro-antioxidant status and affects haematological indices but does not enhance exercise performance. © University of Navarra 2010. Source

Zembron-Lacny A.,University of Physical Education Poznan | Slowinska-Lisowska M.,Wroclaw University | Ziemba A.,Polish Academy of Sciences
Physiological Research

The present study was designed to evaluate the plasma markers of reactive oxygen species (ROS) activity and cytokines, and their relationship with thiol redox status of basketball players during training. Sixteen professional players of the Polish Basketball Extraleague participated in the study. The study was performed during the preparatory period and the play-off round. Markers of ROS activity (lipid peroxidation TBARS, protein carbonylation PC) and reduced glutathione (GSH) demonstrated regularity over time, i.e. TBARS, PC and GSH were elevated at the beginning and decreased at the end of training periods. Oxidized glutathione (GSSG) was not affected by exercise training. Thiol redox status (GSHtotal-2GSSG/GSSG) correlated with TBARS and PC in both training periods. The level of interleukin-6 (IL-6) was increased and positively correlated with thiol redox (r=0.423) in the preparatory period, whereas tumor necrosis factor α (TNFα) was increased and inversely correlated with thiol redox (r=-0.509) in the play-off round. The present study showed significant shifts in markers of ROS activity, thiol redox status and inflammatory mediators (IL-6, TNFα) following professional sport training as well as correlation between changes in thiol redox and cytokine response. © 2010 Institute of Physiology v.v.i., Academy of Sciences of the Czech Republic, Prague, Czech Republic. Source

Ostapiuk-Karolczuk J.,University of Physical Education Poznan | Zembron-Lacny A.,University of Physical Education Poznan | Naczk M.,University of Physical Education in Warsaw | Gajewski M.,Medical University of Gdansk | And 3 more authors.
Journal of Sports Medicine and Physical Fitness

Aim. The goal of the study was to clarify the sequence of cytokines and inflammatory cells in non-athletes performed an intense running exercise. Methods. Sixteen young healthy men participated in the exercise trial that involved 90-min run at 65% VO2max• Results. The plasma concentrations of anti-inflammatory cytokines IL-4, IL-6 and IL-10 increased immediately after exercise simultaneously with number of white blood cells. Between IL-6 and IL-10, and neutrophils the relationships were observed. The correlation value for IL-6 and neutrophils was 0.775 whereas for IL-10 and neutrophils was 0.506. The proinflammatory cytokines IL-I β and TNFa were detected at 6 h after exercise and moderately correlated with monocytes count. The high level of proinflammatory cytokines, monocytes and creatine kinase (CK) remained until 48 h rest. The CK activity significantly correlated with IL-1β (r=0.578) and TNFa (r=0.452), and also with monocytes count (r=0.439). Conclusion. The results have shown that: 1) exercise induces anti-inflammatory cytokines production first and then proinflammatory cytokines; and 2) prolonged proinflammatory response is closely related with muscle damage present. Source

Zembron-Lacny A.,University of Physical Education Poznan | Naczk M.,University of Physical Education Poznan | Gajewski M.,Medical University of Gdansk | Ostapiuk-Karolczuk J.,University of Physical Education Poznan | And 3 more authors.
Physiological Research

The aim of this study was to compare the levels of the plasma muscle-derived cytokines (myokines) and reactive oxygen and nitrogen species (RONS) after muscle damage triggered by different exercises, and to demonstrate the relationships between RONS, thiol redox status and myokines. Sixteen young men participated in a 90-min run at 65 % VO2max (Ex.1) or 90-min run at 65 % VO2max finished with a 15-min eccentric phase (Ex.2, downhill running). Plasma samples were collected before and at 20 min, 24 h and 48 h after exercise. The exercise trials significantly elevated the concentrations of plasma hydrogen peroxide (H2O2) and 8-isoprostane at 20 min rest. Myokines IL-6 and IL-10 increased at 20 min rest while IL-1β and TNFα increased at 24 h rest following both running. Ex.2 caused a significant increase in nitric oxide (NO), IL-6, IL-10 and oxidized glutathione (GSSG) levels. Thiol redox status (GSHtotal-2GSSG/GSSG) decreased by about 30 % after Ex.2 as compared to Ex.1. H2O2 and NO directly correlated with IL-6, IL-10, IL-1β and TNFα and glutathione. These results show that eccentric work is an important factor that enhances the production of RONS and muscle-derived cytokines, and that there is a possible participation of thiol redox status in the release of myokines to blood. © 2010 Institute of Physiology v.v.i., Academy of Sciences of the Czech Republic. Source

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