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Rahman F.-U.,Hazara University | Rahman F.-U.,Shaheed Benazir Bhutto University | Perveen F.,Shaheed Benazir Bhutto University | Rauf T.,Shaheed Benazir Bhutto University | And 6 more authors.
Journal of Animal and Plant Sciences | Year: 2015

The present study was conducted to explore the bats of Khyber Pakhtunkhwa, Pakistan. During the present study 52 bats were collected through the mist nets from FR, Peshawar in District Peshawar and Mazdurabad and Ghanu Deri from District Mardan for a period of two years from July 2011 to May 2013. They were identified on the basis of morphological and cranial measurements. Mean (±SD) of the body mass (BM), head and body length (HBL), forearm length (FA) and wingspan (WS) were identified as 19.09±1.36mm, 65.29±2.20 mm, 68.71±1.05 mm and 197±9.7 mm, respectively.The tail is shorter than the forearm proportionally. While the skull is sagittal and measured ascondylocanine length (CCL) 18.61±0.21mm, greater skull length (GTL) 19.64±0.21 mm and Zygomatic breadth (ZB)12.13±0.15 mm.There were no significant (P<0.05) morphological and cranial variations among all the samples collected from the study area. On the basis of morphological and cranial measurements the species was identified as R. microphyllumfor the first time and after 27 years from the Khyber Pakhtunkhwa. © 2015 Pakistan Agricultural Scientists Forum. All rights reserved.

Ullah S.,Quaid-i-Azam University | Begum M.,University of Malakand | Dhama K.,Indian Veterinary Research Institute | Ahmad S.,University of Malakand | And 2 more authors.
Asian Journal of Animal and Veterinary Advances | Year: 2016

The current study was aimed to investigate the genotoxic effect of an organophosphate pesticide malathion in the gills of a freshwater teleost rohu, Labeo rohita using alkaline Single Cell Gel Electrophoresis (SCGE)/comet assay. The 96 h LC50 of malathion was estimated for rohu in a semi-static system and was found to be 5 µg L-1. Specimens of rohu were exposed to LC50 of malathion. Gill tissues were sampled after 24, 48, 72 and 96 h of exposure. DNA damage was evaluated by studying different indices, including tail length (µm), percentage of DNA in tail, tail moment and olive tail moment using TriTek CometScore™. A linear relation was observed between exposure time and DNA damage in the gill cells. The current study revealed malathion as a potent inducer of DNA damage and comet assay as a reliable and sensitive assay for investigating and detecting DNA damage in vivo, induced in fish by genotoxic pesticides. In order to conserve the vanishing populations of rohu in natural aquatic bodies across the country, indiscriminate use of genotoxic pesticides such as malathion should be minimized. ©2016 Sana Ullah et al.

Midrarullah,University of PeshawarKhyber Pakhtunkhwa | Midrarullah,Shaheed Benazir Bhutto University | Ahmed B.,University of PeshawarKhyber Pakhtunkhwa
Pakistan Journal of Botany | Year: 2015

Field experiment was performed to investigate the growth promoting activities of plant growth promoting rhizobacteria (PGPR) on different growth parameters of maize variety ‘Pahari’. Three bacterial strains Azospirillum brasilense strain R1, Azospirillum lipoferum strain RSWT1 and Pseudomonas strain Ky1 were used to inoculate maize. Plant growth promotion was observed in all inoculated treatments over un-inoculated control which was evident from increase in plant height, number of grains/ear, thousand grain weight and biological yield. Azospirillum lipoferum strain RSWT1 was more effective in plant growth promotion than other strains. The inoculation with Azospirillum lipoferum strain RSWT1 evidenced increase in plant height (8.82%), number of ear/plant (35%), ear length (27%), ear weight (19.4%) and thousand grains weight (11.8%) compared to control treatment. Similarly, Inoculation with Azospirillum brasilense strain R1 result increased in plant height (6.5%), number of ears/plant (45.9%), ear length (23.7%), ear weight (17.25%) and thousand grain yield (9.68%) as compared to control treatments. Higher biological yield (9210 kg/ha) was produced when treatment was done with Azospirillum lipoferum strain RSWT1 followed by treatment with Azospirillum brasilense strain R1 (8960 kg/ha). The study revealed that beneficial strains of PGPR can be used as biofertilizer for maize. © 2015, Pakistan Botanical Society. All rights Reserved.

Ahmad B.,University of PeshawarKhyber Pakhtunkhwa | Khan M.S.,University of Agriculture, Peshawar | Haider A.,PGMI Lady Reading Hospital | Khan I.,University of PeshawarKhyber Pakhtunkhwa
Pakistan Journal of Botany | Year: 2015

Brassica juncea (Czern & Coss., L.) is an important oilseed crop. Since it is attacked by several bacterial and fungal diseases, therefore, we developed an easy and simple protocol for the regeneration and transformation of B. juncea variety RAYA ANMOL to give rise to transgenic plants conferring resistance against various fungal diseases. The transformation was carried out using Agrobacterium with Chitinase gene. This gene was isolated from Streptomyces griseus HUT6037. We used two types of explants for transformation i.e. hypocotyls and cotyledons. Only hypocotyls explants showed good results regarding callus initiation. Different hormonal concentrations were applied i.e. BAP 2, 4 and 6 mgL-1 and NAA 0.1, 0.2 and 0.3 mgL-1. However, high transformation efficiency was observed by supplementing the medium with combination of 2 mgL-1 BAP and 0.2 mgL-1 for initiation of callus. Similarly 10 mgL-1 kanamycin and 200 mgL-1 cefotaxime also proved successful for the selection of transformed callus. In order to confirm the presence of transgenic callus Polymerase chain reaction was performed using specific primers for Chitinase gene. © 2015, Pakistan Botanical Society. All rights reserved.

Ali N.,University of Technology Malaysia | Ajmal S.,University of PeshawarKhyber Pakhtunkhwa | Shah M.,University of PeshawarKhyber Pakhtunkhwa | Ahmed R.,University of Technology Malaysia | And 2 more authors.
Chalcogenide Letters | Year: 2014

Antimony doped tin sulphide thin films were prepared on glass substrate from SnS and Sb2S3 powder by thermal evaporation techniques. The thin films were annealed in argon gas at 250°C for 30 minutes. The films were characterized by X-ray diffraction (XRD), optical microscopy, optical absorption, photoconductivity, and hot-probe techniques. The XRD studies revealed that the annealed films are polycrystalline. The band gap was found to be in the range 2.2-2.6eV along with p-type conductivity. The value of the absorption coefficient is found to be higher than 105 cm-1. © 2014, National Institute R and D of Materials Physics. All right reserved.

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