University of Pardubice | Date: 2015-07-27
The invention relates to a method for separation of biopolymer molecules, particularly biopolymer molecules from the group consisting of mono- a multi-phosphorylated peptides, recombinant peptides/proteins with a polyhistidine tag (His-tag) or with another chemically similar biospecific tag, cysteine-containing peptides/proteins and nucleic acids, in which a biopolymer molecule is bound in a binding solution by a specific binding to a carrier, which contains a core with dimensions in nano- and/or submicro- and/or microscale, which is composed of oxide of at least one transition metal and/or silicon oxide, on whose surface is deposited at least one continuous or non-continuous layer and/or nanoparticles of magnetic metal oxide and/or such nanoparticles are deposited in its inner structure, and subsequently undesirable and non-specifically bound components are washed off at least once from the carrier-bound bio-molecules by a washing solution, whereupon biopolymer molecules are eluted from it by changing pH and/or by using an elution solution. The invention also relates to a carrier for application of this method.
Agency: European Commission | Branch: H2020 | Program: Shift2Rail-RIA | Phase: S2R-OC-IP3-01-2016 | Award Amount: 5.00M | Year: 2016
The overall aim of the S-CODE project is to investigate, develop, validate and initially integrate radically new concepts for switches and crossings that have the potential to lead to increases in capacity, reliability and safety while reducing investment and operating costs. The S-CODE project will identify radically different technology concepts that can be integrated together to achieve significantly improved performance for S&C based around new operating concepts (e.g. super-fast switching, self-healing switch). The project will build on existing European and national research projects (in particular, the lighthouse project In2Rail, Capacity4Rail and Innotrack) to bring together technologies and concepts that will significantly reduce the constraints associated with existing switch technologies and develop a radically different solution. The project will be divided into three phases: Phase 1: Requirements and initial design - focusing on understanding constraints and critical requirements, and developing a radically different architecture and operation that makes use of technologies from other domains; Phase 2: Technical development - undertaking detailed modelling and simulation to identify an optimal configuration to maximise performance; Phase 3: Validation and evaluation - testing (to TRL4) the design concepts and formally evaluating their performance in order that an integrated design can be presented for further development.
Agency: European Commission | Branch: H2020 | Program: IA | Phase: ICT-28-2015 | Award Amount: 3.47M | Year: 2016
The development of new methodologies advancing the state of the art in foodborne pathogen detection is a challenge for scientists and technologists as well as food industry and consumers. This project aims to meet the challenge by providing a reliable and versatile solution thanks to the convergence of micro-nano-bio systems. The work capitalizes on several innovative concepts which have already been proven to meet the required criteria for fast, low cost and highly sensitive analysis of pathogens in food samples in a previous research project entitled LoveFood. These concepts are gathered on a credit-card size Lab-on-Chip platform, where all necessary steps for bacteria detection are performed on several chips. Specifically, bacteria capture and lysis (one chip), DNA extraction (second chip) and amplification (third chip) and finally pathogenic-DNA detection (fourth chip) can be performed in less than 8 hours and without the need for skilled personnel or large, lab-based dedicated equipment. To proceed for a higher Technology Readiness Level towards the successful commercialization of the current prototype and produce a portable, and rapid platform (targeting total pathogen analysis time 4 hours including a 3 hour pre-enrichment step), we propose to further develop it by integrating the bacteria lysis, DNA purification and amplification modules, as well as the biochip detection platform on a single cartridge, able to perform multi-pathogen analysis (i.e. Salmonella, Listeria, Escherichia coli and Bacillus cereus) in multiple samples. The system will be developed for dairy products and meat analysis, with a strong commitment to produce a pre-industrial prototype by the end of the project.
Agency: European Commission | Branch: H2020 | Program: IA | Phase: GALILEO-3-2015 | Award Amount: 1.68M | Year: 2016
RHINOS aims at increasing the use of EGNSS to support the safety-critical train localization function for train control in emerging regional and global markets. RHINOS adds value to EGNSS by leveraging the results from prior or existing projects, and develops a Railway High Integrity Navigation Overlay System to be used by the rail community. RHINOS pillar is the GNSS infrastructure realized for the aviation application with additional layers that meet the rail requirements in the difficult railway environments. RHINOS will feature an international cooperation with the Stanford University that has been involved in the aviation application since the birth of the GPS, gaining an undeniable knowledge of the GNSS performance and high-integrity applications. The ambition is a positive step beyond the proliferation of GNSS platforms, mainly tailored for regional applications, to favor a global solution to release the potential benefits of the EGNSS in the fast growing train signaling world market. The RHINOS work programme includes the investigation of candidate concepts for the provision of the high integrity needed to protect the detected position of the train, as required by the train control system application. The EGNSS (GALILEO and EGNOS) plus GPS and WAAS constitute the reference infrastructure that is available world-wide. Moreover, local augmentation elements, ARAIM techniques and other sensors on the train are the add-on specific assets for mitigating the hazards due to the environmental effects which dominate the rail application. A further objective of RHINOS is to contribute to the definition of a standard for the Railway High Integrity Navigation Overlay System leveraging on the EU-US Cooperation Agreement on ARAIM. The RHINOS dissemination plan includes three specific Workshops with the rail and satellite stakeholders, at Stanford University for the US community, in Roma for the Western European community and in Prague for the Eastern European community.
Synpo, University of Pardubice and R.Ø.S.A. | Date: 2014-12-24
The invention relates to a method of preparation of a stabilized polyethylene (PE), in which the polyethylene (PE) is first plasma-treated, by which means hydroxyl and/or amine functional groups are formed on the surface of PE, to which in the following step is either bound methacryoyl chloride, capable of copolymerization with a polymerizable stabilizer, or to the hydroxyl and/or amine functional groups of the treated PE by its -NCO functional groups is bound an additive with reactive -NCO groups, to which at least one stabilizer is subsequently bound by its -OH, -NH or -NH_(2) groups.
Svoboda R.,University of Pardubice
Acta Materialia | Year: 2013
Structural relaxation processes were studied by means of differential scanning calorimetry for a number of compositions belonging to the Se-rich sides of Se-Te, Ge-Se and As-Se chalcogenide systems. Based on the results of fitting and non-fitting methods, the relaxation behavior of these selenide glasses is described in terms of the Tool-Narayanaswamy-Moynihan (TNM) equations. The goal of this paper is to introduce an advanced model describing the relationship between the macroscopic relaxation behavior and molecular structure of the glass. Special attention is paid to differences between the particular structural units and molecular arrangements characteristic for each system studied. The TNM parameters as well as their changes are discussed with respect to both the increasing content of dopant in the Se matrix within each chalcogenide system and different structural units employed in the respective systems. A unique novel conception is suggested, showing a connection between the values of TNM parameters and respective molecular structures and their changes during structural relaxation. © 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Bures F.,University of Pardubice
RSC Advances | Year: 2014
Property tuning in selected examples of D-π-A molecules has been discussed and summarized in this review article. The tuning and structure-property relationships have been demonstrated on the particular A, π and D parts of the push-pull molecule. Special emphasis has been put on the tuning of the FMO levels and optical properties. Further prospective applications of the given chromophore have also been considered. This journal is © 2014 The Royal Society of Chemistry.
Jandera P.,University of Pardubice
Journal of Chromatography A | Year: 2013
Monolithic continuous separation media are gradually finding their way to sample pre-treatment, isolation, enrichment and final analytical separations of a plethora of compounds, occurring as food components, additives or contaminants, including pharmaceuticals, pesticides and toxins, which have traditionally been the domain of particulate chromatographic materials. In the present review, recent advances in the technology of monolithic columns and the applications in food analysis are addressed. Silica-based monoliths are excellent substitutes to conventional particle-packed columns, improving the speed of analysis for low-molecular weight compounds, due to their excellent efficiency and high permeability. These properties have been recently appreciated in two-dimensional HPLC, where the performance in the second dimension is of crucial importance. Organic-polymer monoliths in various formats provide excellent separations of biopolymers. Thin monolithic disks or rod columns are widely employed in isolation, purification and pre-treatment of sample containing proteins, peptides or nucleic acid fragments. Monolithic capillaries were originally intended for use in electrochromatography, but are becoming more frequently used for capillary and micro-HPLC. Monoliths are ideal highly porous support media for immobilization or imprinting template molecules, to provide sorbents for shape-selective isolation of target molecules from various matrices occurring in food analysis. The separation efficiency of organic polymer monoliths for small molecules can be significantly improved by optimization of polymerization approach, or by post-polymerization modification. This will enable full utilization of a large variety of available monomers to prepare monoliths with chemistry matching the needs of selectivity of separations of various food samples containing even very polar or ionized compounds. © 2013 Elsevier B.V.
Jandera P.,University of Pardubice
Journal of Chromatography A | Year: 2012
Recent advances in applications of gradient elution in two-dimensional liquid chromatography are reviewed. Benefits of simultaneous programmed elution in two-dimensional liquid chromatography are shown, with emphasis on fast gradient elution in second dimension using various gradient types and profiles. The theory of gradient elution is shortly overviewed with attention to its impacts on two-dimensional separations. Various two-dimensional gradient systems are compared in terms of peak capacity and peak production rate: off-line, stop-and-go and real-time comprehensive approaches. Selection of stationary phases in combination with gradient elution and the effects of gradient elution on improved regularity of two-dimensional space coverage, orthogonality and peak capacity are discussed. Not only two-dimensional reversed-phase systems, but also other HPLC modes such as HILIC, ion-exchange or normal-phase chromatography with organic mobile phases are included. Fraction modulation in gradient elution setups, mobile phase compatibility and solvent effects in the fraction transfer between the first and the second dimension are addressed. Design and optimization of two-dimensional gradient separation systems for particular ordered and non-ordered samples are dealt with in detail. The discussed principles are illustrated by practical examples. © 2012 Elsevier B.V.
Agency: European Commission | Branch: H2020 | Program: ERC-STG | Phase: ERC-StG-2014 | Award Amount: 1.64M | Year: 2015
In photovoltaics (PVs), a significant scientific and technological attention has been given to technologies that have the potential to boost the solar-to-electricity conversion efficiency and to power recently unpowerable devices and objects. The research of various solar cell concepts for diversified applications (building integrated PVs, powering mobile devices) has recently resulted in many innovations. However, designs and concepts of solar cells fulfilling stringent criteria of efficiency, stability, low prize, flexibility, transparency, tunable cell size, esthetics, are still lacking. Herein, the research focus is given to a new physical concept of a solar cell that explores extremely promising materials, yet unseen and unexplored in a joint device, whose combination may solve traditional solar cells drawbacks (carrier recombination, narrow light absorption). It features a high surface area interface (higher than any other known PVs concept) based on ordered anodic TiO2 nanotube arrays, homogenously infilled with nanolayers of high absorption coefficient crystalline chalcogenide or organic chromophores using different techniques, yet unexplored for this purpose. After addition of supporting constituents, a solid-state solar cell with an extremely large incident area for the solar light absorption and optimized electron pathways will be created. The CHROMTISOL solar cell concept bears a large potential to outperform existing thin film photovoltaic technologies and concepts due to unique combination of materials and their complementary properties. The project aims towards important scientific findings in highly interdisciplinary fields. Being extremely challenging and in the same time risky, it is based on feasible ideas and steps, that will result in exciting achievements. The principal investigator, Jan Macak, has an outstanding research profile in the field of self-organized anodic nanostructures and is an experienced researcher in the photovoltaic field