University of Medicine and Pharmacy Iuliu Hatieganu

Medicine and, Romania

University of Medicine and Pharmacy Iuliu Hatieganu

Medicine and, Romania
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PubMed | University of Medicine and Pharmacy, Cluj-Napoca and University of Medicine and Pharmacy Iuliu Hatieganu
Type: Journal Article | Journal: Journal of gastrointestinal and liver diseases : JGLD | Year: 2014

A gradual shift of colorectal adenoma and carcinoma location toward the proximal colon has been recently observed both in the United States and Europe. We aimed to study the polyp and adenoma detection rate in a major endoscopy center of northwestern Romania over a 16-year period, and to characterize the distribution and the pathological features of the removed polyps according to age and sex, in order to assess the trend of proximal adenoma prevalence in our population.We retrospectively analyzed 9,230 consecutive colonoscopies performed between 1996 and 2011 in a high-volume outpatient clinic in Cluj-Napoca, Romania. We analyzed 2,436 complete colonoscopies that detected 3,642 polyps in two time periods: 1996 to 2003 and 2004 to 2011. We compared the number, size and histopathological features of the polyps removed from the right-sided colon and the left sided-colon in the two periods.An increasing trend of polyp detection rate in the right-sided colon was observed, from 9.36% in the first period to 12.17% in the second period (p<0.001). The prevalence of right-sided colon adenomas also presented an increased trend (OR 1.45; CI95% 1.02-2.05; p=0.03). High-grade dysplasia (HGD) was found in 8.6% of the adenomas and in 4.1% of the diminutive polyps. Advanced neoplasia was detected in 1.5% of persons younger than 50 years. Multivariate logistic regression analysis evidenced that the right-sided polyps were significantly associated with the last time period (OR 1.3; p=0.001; CI95% 1.12-1.56), male gender (OR-1.3; p=0.001; CI95% 1.1-1.5) and age above 48 years (OR 1.3; p=0.006: CI95% 1-1.6).An increasing trend of polyp detection rate in the right-sided colon was documented, with an increasing prevalence of right-sided adenomas. The evaluation of the proximal colon is particularly important in males aged over 48. A clear-cut risk of HGD in the diminutive polyps and in the middle age subjects has been also observed.

Wykes T.,King's College London | Reeder C.,King's College London | Huddy V.,King's College London | Taylor R.,King's College London | And 5 more authors.
Schizophrenia Research | Year: 2012

Background: Cognitive remediation (CRT) affects functioning but the extent and type of cognitive improvements necessary are unknown. Aim: To develop and test models of how cognitive improvement transfers to work behaviour using the data from a current service. Method: Participants (N49) with a support worker and a paid or voluntary job were offered CRT in a Phase 2 single group design with three assessments: baseline, post therapy and follow-up. Working memory, cognitive flexibility, planning and work outcomes were assessed. Results: Three models were tested (mediation - cognitive improvements drive functioning improvement; moderation - post treatment cognitive level affects the impact of CRT on functioning; moderated mediation - cognition drives functioning improvements only after a certain level is achieved). There was evidence of mediation (planning improvement associated with improved work quality). There was no evidence that cognitive flexibility (total Wisconsin Card Sorting Test errors) and working memory (Wechsler Adult Intelligence Scale III digit span) mediated work functioning despite significant effects. There was some evidence of moderated mediation for planning improvement if participants had poorer memory and/or made fewer WCST errors. The total CRT effect on work quality was d = 0.55, but the indirect (planning-mediated CRT effect) was d = 0.082. Conclusion: Planning improvements led to better work quality but only accounted for a small proportion of the total effect on work outcome. Other specific and non-specific effects of CRT and the work programme are likely to account for some of the remaining effect. This is the first time complex models have been tested and future Phase 3 studies need to further test mediation and moderated mediation models. © 2012 Elsevier B.V.

Micu M.C.,County Hospital Turda | Micu M.C.,Clinical Rehabilitation Hospital Cluj | Bogdan G.D.,County Hospital | Fodor D.,University of Medicine and Pharmacy Iuliu Hatieganu
Rheumatology | Year: 2010

Objective: To determine the efficacy of IA corticosteroid (CS) injection in pain reduction for hip OA under ultrasound (US) guidance. Methods: Forty patients [mean age 62.78 (8.16) years] fulfilling ACR criteria for hip OA, with synovitis detected at US, gave their consent for IA US-guided CS injection because of pain refractory to conventional therapy. At baseline, at 1 and 3 months, patients filled up a visual analogue scale (VAS) pain on walking, performed the Lequesne index and were checked by US for synovitis. Results were compared with age-matched controls. The occurrence of side effects both short and long term was monitored. Results: IA steroid deposition was performed under US guidance. After 1 and 3 months, walking pain VAS was significantly reduced vs baseline (P < 0.001) and had high correlation with Lequesne index. Synovial hypertrophy was reduced in 75% of the hips after 1 and 3 months vs baseline (P < 0.001). In the group of controls, hip walking pain VAS, Lequesne index and synovial hypertrophy were not changed at 3 months vs baseline (P > 0.05). Transient facial rash was present in 16 patients during the first 24-48 h after injection. No side effects were reported. Conclusion: US-guided steroid injections in hip OA is an efficacious and safe therapeutic approach to achieve pain control and reduction of synovial hypertrophy avoiding the use of X-ray-guided procedure. © The Author 2010.

PubMed | University of Medicine and Pharmacy Iuliu Hatieganu, University of Medicine and Pharmacy of Craiova and Laboratories of Phytotherapy and Homeopathy
Type: Journal Article | Journal: Current health sciences journal | Year: 2014

THE PURPOSE OF THIS STUDY WAS THE QUALITATIVE AND QUANTITATIVE ANALYSIS OF FLAVONOIDS FROM ROBINIA PSEUDOACACIA USING TWO DIFFERENT TECHNIQUES OF ANALYSIS: Thin Layer Chromatography (TLC) and TLC coupled with photo-densitometry. The results obtained by chromatographic analysis showed a higher concentration of flavonoids in flowers than in leaves. The flowers harvested in the plains have a higher concentration of hyperoside (0.9 mg/mL) compared with the flowers collected from the hills (0.54 mg/mL). The leaves are richer in ruthoside (0.98 mg/mL) compared with the flowers.

Berindan-Neagoe I.,Oncological Institute Ion Chiricuta | Berindan-Neagoe I.,University of Medicine and Pharmacy Iuliu Hatieganu | Berindan-Neagoe I.,Victor Babes University of Medicine and Pharmacy Timisoara | Braicu C.,Oncological Institute Ion Chiricuta | And 9 more authors.
Journal of Gastrointestinal and Liver Diseases | Year: 2013

Background & Aims. The present study was designed to examine the combined effects of Oxaliplatin (OXA) and 5-Fluorouracil (5-FU) in the Colo320 cell line. Methods. The antiproliferative effects were evaluated using the MTT assay, apoptosis by flow cytometry, and RT-PCR-array technology was used to determine the major effects of the two chemotherapeutic drugs upon the most important genes involved in apoptosis. Results. The antiproliferative effects of the therapeutic agents, as individual therapy or combined, proved to be dose and time-dependent, with increased efficiency for the combined treatment. Flow cytometry data revealed increased apoptotic processes in the case of the combined treatment at 24 hours after administration. The RT-PCR-array data indicated that at 24 hours after OXA treatment, 49 genes were differentially expressed, of which 45 were up-regulated and 4 down-regulated. In the case of the 5-FU treatment, 35 genes were down regulated and 2 up regulated. In the combined treatment of 5-FU and OXA, 19 genes were up-regulated and 15 down-regulated. Conclusions. This study proved that drug resistance could be counteracted by combining OXA with 5-FU to form a tandem that is capable of reducing cell proliferation and to stimulate extrinsic apoptosis pathway by targeting death receptors on the cell surface.

Vlase L.,University of Medicine and Pharmacy Iuliu Hatieganu | Popa A.,University of Medicine and Pharmacy Iuliu Hatieganu | Neag M.,1st Medical Clinic | Muntean D.,University of Medicine and Pharmacy Iuliu Hatieganu | And 2 more authors.
Clinical and Experimental Pharmacology and Physiology | Year: 2012

1.Our objective was to evaluate a possible pharmacokinetic interaction between zolpidem and fluvoxamine in healthy volunteers. 2.The study consisted of two periods: Period1 (reference), when each volunteer received a single dose of 5mg zolpidem; and Period2 (test), when each volunteer received a single dose of 5mg zolpidem and 100mg fluvoxamine. Between the two periods, the subjects were treated for 6days with a single daily dose of 100mg fluvoxamine. 3.Pharmacokinetic parameters of zolpidem given in each treatment period were calculated using non-compartmental analysis and the data from two periods were compared to determine statistically significant differences. 4.In the two periods of treatments, the mean peak plasma concentrations (C max) were 56.4±25.6ng/mL (zolpidem alone) and 67.3±25.8ng/mL (zolpidem after pretreatment with fluvoxamine). The t max, times taken to reach C max, were 0.83±0.44 and 1.26±0.74h, respectively, and the total areas under the curve (AUC 0-∞) were 200.9±116.8 and 512.0±354.6ngh/mL, respectively. The half-life of zolpidem was 2.24±0.81h when given alone and 4.99±2.92h after pretreatment with fluvoxamine. 5.Fluvoxamine interacts with zolpidem in healthy volunteers and increases its exposure by approximately 150%. The experimental data showed the pharmacokinetic interaction between zolpidem and fluvoxamine, and suggest that the observed interaction might be clinically significant, but its relevance has to be confirmed. © 2011 The Authors. Clinical and Experimental Pharmacology and Physiology © 2011 Blackwell Publishing Asia Pty Ltd.

Ivanescu B.,Grigore T. Popa University of Medicine and Pharmacy | Vlase L.,University of Medicine and Pharmacy Iuliu Hatieganu | Corciova A.,Grigore T. Popa University of Medicine and Pharmacy | Lazar M.I.,Grigore T. Popa University of Medicine and Pharmacy
Natural Product Research | Year: 2011

Artemisinin, a sesquiterpene lactone from Artemisia annua L., has received considerable attention in the last few decades as a potent antimalarial drug. Artemisinin has rather low toxicity; it is effective against drug-resistant Plasmodium species and against cerebral malaria. This study reports the development of a rapid and sensitive assay for the quantification of artemisinin in A. annua by reversed phase HPLC/MS. In the selected optimal experimental conditions, artemisinin exhibited a well-defined chromatographic peak with a retention time of 2 ±0.2 min. The chromatographic signal shows a linear dependence with artemisinin concentration, enabling the use of this signal for artemisinin quantification according to the following regression equation: y = 2665.40x-14697.61. The correlation coefficient (R 2) was 0.9989. For every concentration within the range of the standard curve (0.1-2 μg mL -1), accuracy was between 95 and 104%. Artemisinin content in Romanian A. annua wild plants varies between 0.17 and 0.21% (dry weight basis). © 2011 Taylor &Francis.

Miclea D.,University Of Medicine And Pharmacy Iuliu Hatieganu | Pop I.V.,University Of Medicine And Pharmacy Iuliu Hatieganu
Annals of the Romanian Society for Cell Biology | Year: 2012

Turner syndrome is very heterogeneous, short stature often being the only clinical sign. In this pathology, classical karyotype is the gold standard in genetic investigation for the positive diagnosis, and it is recommended to be performed in every girl with unexplained short stature. We investigated by standard and molecular cytogenetics a girl who presented for short stature not explained by common medical reasons. Standard karyotype showed homogeneous monosomy, 45,X, FISH technique revealing a mosaicism undetected before, 45,X/46,XX, the level of mosaicism for the second cell line being of 22%. This difference could be explained by an augmented number of cells analyzed by FISH, thus improving the cytogenetic diagnosis. FISH technique is useful in medical practice, complementary to the classical techniques due to a greater resolution and for the detection of the mosaicism.

Sparchez Z.,University of Medicine and Pharmacy Iuliu Hatieganu | Sparchez Z.,Institute for Gastroenterology and Hepatology | Radu P.,University of Medicine and Pharmacy Iuliu Hatieganu | Sparchez M.,University of Medicine and Pharmacy Iuliu Hatieganu | And 6 more authors.
Journal of Gastrointestinal and Liver Diseases | Year: 2013

The era of the real time low mechanical index (MI) contrast enhanced ultrasound (CEUS) began in 2004. Since then, CEUS with second generation contrast agents like SonoVue has been able to offer a new clinical utility both in diagnosis and in interventional therapies. Intracavitary administration of SonoVue is an off-label, extravascular application of CEUS. There are two distinct applications in gastroenterology that are currently emerging: contrast agent injection into physiological cavities and injection into non-physiological cavities and fistulas. Numerous reports on the extravascular or intracavitary administration of SonoVue have been published and the results are positive, even though larger prospective studies are still lacking.

Tantau M.,University of Medicine and Pharmacy Iuliu Hatieganu | Tantau M.,Regional Institute of Gastroenterology and Hepatology Octavian Fodor | Procopet B.,University of Medicine and Pharmacy Iuliu Hatieganu | Procopet B.,Regional Institute of Gastroenterology and Hepatology Octavian Fodor | Bureau C.,University Paul Sabatier
Journal of Gastrointestinal and Liver Diseases | Year: 2013

Portal hypertension is a major consequence of any chronic liver disease and it represents the main mechanism of complication occurrence. Therefore, the assessment of portal hypertension presence is one of the most important steps in the management of any chronic liver diseases. The most accurate tool for portal pressure assessment is hepatic venous pressure gradient (HVPG) measurement, which has diagnostic and prognostic relevance. In this paper we review the methodology of HVPG measuring, together with the clinical relevance of this technique. Portal hypertension is denned as a HVPG higher than 5 mmHg, but clinically significant portal hypertension that predisposes to clinical decompensation is defined as HVPG higher than 10 mmHg. HVPG is useful for portal hypertension treatment monitoring. A decrease in HVPG greater than 20% or under the threshold of 12 mmHg is considered to be protective against portal hypertension-related events. Even if HVPG measurement is a safe procedure, it is still considered an invasive technique and not widely available. Therefore, non-invasive markers of portal hypertension were searched for. Until now only liver stiffness measurement by transient elastography has proved to be sufficiently accurate but there is still heterogeneity among the cut-off values for portal hypertension diagnosis.

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