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Silosi I.,University of Medicine and Pharmacy of Craiova | Cojocaru M.,Titu Maiorescu University | Foia L.,University of Medicine and Pharmacy of Iasi | Boldeanu M.V.,University of Medicine and Pharmacy of Craiova | And 3 more authors.
Journal of Immunology Research

In the recent years, statistically significant associations between rheumatoid arthritis (RA) and periodontal disease have been identified. Emerging as a chronic inflammatory joint disease, RA displays various features and pathogenetic events similar to chronic periodontitis (CP). The purpose of this study was to evaluate the utility of determining systemic and crevicular levels of metalloproteinase-9 (MMP-9) as potential biomarkers for association between RA and CP. A total of fifty-six patients were included in the study. The subjects were categorized into four groups as follows: healthy-control (n = 21), active RA (n = 16), CP (n = 14), and RA-CP association (n = 12). Assessment of serum and crevicular concentrations of total MMP-9 (active and pro-MMP-9) was based on ELISA technique. The results of this study showed statistically significant differences of serum MMP-9 between patients groups and control. Serum levels of MMP-9 were similar in RA and RA-CP associated patients. Gingival crevicular fluid (GCF) recorded increased MMP-9 levels in RA-CP association subjects as compared to CP. Considering that RA-CP association is characterized by a disregulation of the inflammatory response, MMP-9 may play a role in the pathogenesis of RA-CP association. MMP-9 is therefore a sensitive tool in the diagnosis and management of patients affected by this binomial association. © 2015 Isabela Silosi et al. Source

Kloetzer L.,Technical University Gheorghe Asachi | Postaru M.,Technical University Gheorghe Asachi | Galaction A.-I.,University of Medicine and Pharmacy of Iasi | Blaga A.C.,Technical University Gheorghe Asachi | Cascaval D.,Technical University Gheorghe Asachi
Industrial and Engineering Chemistry Research

The mechanism of reactive extraction of rosmarinic acid with Amberlite LA-2 (secondary amine) and D2EHPA (organophosphoric acid) was analyzed for three solvents with different polarities (n-heptane, n-butyl acetate, dichloromethane), with or without 1-octanol. Thus, in the absence of 1-octanol, the stoichiometric ratio between the rosmarinic acid and Amberlite LA-2 was 1:1 for dichloromethane, 1:2 for butyl acetate, and 1:4 for n-heptane. In the presence of 1-octanol, the formation of aminic adducts is restricted, and the structures of the interfacial complexes reflect the following stoichiometries for the interfacial reaction between the solute and Amberlite LA-2: 1:1 for dichloromethane and butyl acetate and 1:2 for n-heptane. However, regardless of 1-octanol addition to the organic phase, the extraction of rosmarinic acid with D2EHPA occurred only through the formation of an interfacial compound containing one acid molecule and two molecules of extractant for all considered solvents. In the case of reactive extraction with Amberlite LA-2, the highest extraction constant was reached for low-polarity solvents, which promotes the formation of aminic adducts. In contrast, an increase of the organic-phase polarity positively influenced the value of the extraction constant for extraction with D2EHPA. © 2013 American Chemical Society. Source

Vlase L.,University of Medicine and Pharmacy, Cluj-Napoca | Vancea S.,University of Medicine and Pharmacy of Targu Mures | Mircioiu C.,Carol Davila University of Medicine and Pharmacy | Lupusoru C.E.,University of Medicine and Pharmacy of Iasi | And 2 more authors.
Studia Universitatis Babes-Bolyai Chemia

A rapid and sensitive liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) method for the quantification of ethambutol in human plasma was developed and validated. The separation was performed on a Gemini C18 column under isocratic conditions using a mixture of methanol and 0.1% ammonia 65:35 (v/v) as mobile phase, at 40 °C with a flow rate of 0.6 mL/min. The detection of ethambutol was performed in multiple reaction monitoring mode using an ion trap mass spectrometer with atmospheric pressure chemical ionization, operating in positive MS/MS mode. The human plasma samples (0.1 mL) were deproteinized with methanol and aliquots of 2 μL from supernatants obtained after centrifugation were directly injected into the chromatographic system. The method shows a good linearity (r > 0.994), precision (CV < 8.8%) and accuracy (bias < 12.3%) over the range of 0.36- 17.18 μg/mL plasma. The lower limit of quantification (LLOQ) was 0.36 μg/mL and the recovery was between 93-111%. The developed and validated method is simple, rapid and specific for the determination of ethambutol in human plasma and was successfully applied in therapeutic drug monitoring of ethambutol in patients with tuberculosis. Source

Cobzeanu M.D.,University of Medicine and Pharmacy of Iasi | Drug V.,University of Medicine and Pharmacy of Iasi | Palade O.D.,University of Medicine and Pharmacy of Iasi | Ciobotaru A.,University of the Arts George Enescu of Iasi | Voineag M.,University of Siegen
EPE 2012 - Proceedings of the 2012 International Conference and Exposition on Electrical and Power Engineering

The authors realized a prospective study on a series of 96 patients working in different fields requiring intense vocal effort, analyzing the influence of different environmental factors and of the gastroesophageal reflux in generating pharyngolaryngeal inflammatory pathology followed by a professional dysphonia. They insisted upon the use of the standard including questionnaire and the methods of investigation for the vocal parameters during the gastroesophageal reflux disease (GERD). Alcohol intake, smoking, stress, gastro esophageal reflux and different allergens influenced the symptoms and the objective alterations of the vocal performance (Reflux Finding Score - RFI, Reflux Symptoms Index - RSI). The symptoms, statistically significant, on the studied series were hoarseness (p=0.0l), postnasal drip (p=0.03), globus hystericus (p=0.0l). ENT examination revealed: erythema, congestion (p=0.0l), vocal folds edema (p=0.0l), profuse laryngeal edema (p=0.02), posterior commissure hypertrophy (p=0.02). Environmental factors and gastro esophageal reflux disease had a negative impact on the vocal performances of the vocal professionals. © 2012 IEEE. Source

Solomon S.,University of Medicine and Pharmacy of Iasi | Ursarescu I.,University of Medicine and Pharmacy of Iasi | Martu A.,University of Medicine and Pharmacy of Iasi | Luchian I.,University of Medicine and Pharmacy of Iasi | And 3 more authors.
Revista de Chimie

The periodontal disease represents an inflammatory disease with an infectious etiology; the photo-activated disinfection of the periodontal pockets was proposed as an adjunctive form of treatment to the classic mechanical scaling and root planning. The purpose of our study is to assess the efficiency of the photo-activated toluidine blue on the clinical parameters of the patient with chronic periodontitis versus classic mechanical treatment alone. Source

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