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Kloetzer L.,Technical University Gheorghe Asachi | Postaru M.,Technical University Gheorghe Asachi | Galaction A.-I.,University of Medicine and Pharmacy of Iasi | Blaga A.C.,Technical University Gheorghe Asachi | Cascaval D.,Technical University Gheorghe Asachi
Industrial and Engineering Chemistry Research | Year: 2013

The mechanism of reactive extraction of rosmarinic acid with Amberlite LA-2 (secondary amine) and D2EHPA (organophosphoric acid) was analyzed for three solvents with different polarities (n-heptane, n-butyl acetate, dichloromethane), with or without 1-octanol. Thus, in the absence of 1-octanol, the stoichiometric ratio between the rosmarinic acid and Amberlite LA-2 was 1:1 for dichloromethane, 1:2 for butyl acetate, and 1:4 for n-heptane. In the presence of 1-octanol, the formation of aminic adducts is restricted, and the structures of the interfacial complexes reflect the following stoichiometries for the interfacial reaction between the solute and Amberlite LA-2: 1:1 for dichloromethane and butyl acetate and 1:2 for n-heptane. However, regardless of 1-octanol addition to the organic phase, the extraction of rosmarinic acid with D2EHPA occurred only through the formation of an interfacial compound containing one acid molecule and two molecules of extractant for all considered solvents. In the case of reactive extraction with Amberlite LA-2, the highest extraction constant was reached for low-polarity solvents, which promotes the formation of aminic adducts. In contrast, an increase of the organic-phase polarity positively influenced the value of the extraction constant for extraction with D2EHPA. © 2013 American Chemical Society.


Silosi I.,University of Medicine and Pharmacy of Craiova | Cojocaru M.,Titu Maiorescu University | Foia L.,University of Medicine and Pharmacy of Iasi | Boldeanu M.V.,University of Medicine and Pharmacy of Craiova | And 3 more authors.
Journal of Immunology Research | Year: 2015

In the recent years, statistically significant associations between rheumatoid arthritis (RA) and periodontal disease have been identified. Emerging as a chronic inflammatory joint disease, RA displays various features and pathogenetic events similar to chronic periodontitis (CP). The purpose of this study was to evaluate the utility of determining systemic and crevicular levels of metalloproteinase-9 (MMP-9) as potential biomarkers for association between RA and CP. A total of fifty-six patients were included in the study. The subjects were categorized into four groups as follows: healthy-control (n = 21), active RA (n = 16), CP (n = 14), and RA-CP association (n = 12). Assessment of serum and crevicular concentrations of total MMP-9 (active and pro-MMP-9) was based on ELISA technique. The results of this study showed statistically significant differences of serum MMP-9 between patients groups and control. Serum levels of MMP-9 were similar in RA and RA-CP associated patients. Gingival crevicular fluid (GCF) recorded increased MMP-9 levels in RA-CP association subjects as compared to CP. Considering that RA-CP association is characterized by a disregulation of the inflammatory response, MMP-9 may play a role in the pathogenesis of RA-CP association. MMP-9 is therefore a sensitive tool in the diagnosis and management of patients affected by this binomial association. © 2015 Isabela Silosi et al.


PubMed | University of Medicine and Pharmacy of Iasi, University of Medicine and Pharmacy of Craiova and Titu Maiorescu University
Type: | Journal: Journal of immunology research | Year: 2015

In the recent years, statistically significant associations between rheumatoid arthritis (RA) and periodontal disease have been identified. Emerging as a chronic inflammatory joint disease, RA displays various features and pathogenetic events similar to chronic periodontitis (CP). The purpose of this study was to evaluate the utility of determining systemic and crevicular levels of metalloproteinase-9 (MMP-9) as potential biomarkers for association between RA and CP. A total of fifty-six patients were included in the study. The subjects were categorized into four groups as follows: healthy-control (n = 21), active RA (n = 16), CP (n = 14), and RA-CP association (n = 12). Assessment of serum and crevicular concentrations of total MMP-9 (active and pro-MMP-9) was based on ELISA technique. The results of this study showed statistically significant differences of serum MMP-9 between patients groups and control. Serum levels of MMP-9 were similar in RA and RA-CP associated patients. Gingival crevicular fluid (GCF) recorded increased MMP-9 levels in RA-CP association subjects as compared to CP. Considering that RA-CP association is characterized by a disregulation of the inflammatory response, MMP-9 may play a role in the pathogenesis of RA-CP association. MMP-9 is therefore a sensitive tool in the diagnosis and management of patients affected by this binomial association.


PubMed | University of Medicine and Pharmacy of Iasi, University of Medicine and Pharmacy of Targu Mures and University of Medicine and Pharmacy of Craiova
Type: Journal Article | Journal: Current health sciences journal | Year: 2015

Purpose. Craniofacial morphology is usualy described by the head (cephalic index) and face form (prosopic index). The aim of this study was to establish the existence of correlations between the cranial and facial types and the dento-maxillary anomalies. Material and Methods. Data were collected from two groups of patients (a control group of 42 patients without dental anomalies and a test group of 76 patients with dento-maxillary anomalies) of both sexes and age range 11-16 years. Results. Both in the control and test groups, 50% of patients were classified in mesocephalic type and mesoprosopic type. Conclusions. Groups studied by us showed correlations between anomalies in the transverse plane and dolichocephalic type and correlations between anomalies in the sagittal plane and leptoprosopic type.


Vlase L.,University of Medicine and Pharmacy, Cluj-Napoca | Vancea S.,University of Medicine and Pharmacy of Targu Mures | Mircioiu C.,Carol Davila University of Medicine and Pharmacy | Lupusoru C.E.,University of Medicine and Pharmacy of Iasi | And 2 more authors.
Studia Universitatis Babes-Bolyai Chemia | Year: 2011

A rapid and sensitive liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) method for the quantification of ethambutol in human plasma was developed and validated. The separation was performed on a Gemini C18 column under isocratic conditions using a mixture of methanol and 0.1% ammonia 65:35 (v/v) as mobile phase, at 40 °C with a flow rate of 0.6 mL/min. The detection of ethambutol was performed in multiple reaction monitoring mode using an ion trap mass spectrometer with atmospheric pressure chemical ionization, operating in positive MS/MS mode. The human plasma samples (0.1 mL) were deproteinized with methanol and aliquots of 2 μL from supernatants obtained after centrifugation were directly injected into the chromatographic system. The method shows a good linearity (r > 0.994), precision (CV < 8.8%) and accuracy (bias < 12.3%) over the range of 0.36- 17.18 μg/mL plasma. The lower limit of quantification (LLOQ) was 0.36 μg/mL and the recovery was between 93-111%. The developed and validated method is simple, rapid and specific for the determination of ethambutol in human plasma and was successfully applied in therapeutic drug monitoring of ethambutol in patients with tuberculosis.


Cobzeanu M.D.,University of Medicine and Pharmacy of Iasi | Drug V.,University of Medicine and Pharmacy of Iasi | Palade O.D.,University of Medicine and Pharmacy of Iasi | Ciobotaru A.,University of the Arts George Enescu of Iasi | Voineag M.,University of Siegen
EPE 2012 - Proceedings of the 2012 International Conference and Exposition on Electrical and Power Engineering | Year: 2012

The authors realized a prospective study on a series of 96 patients working in different fields requiring intense vocal effort, analyzing the influence of different environmental factors and of the gastroesophageal reflux in generating pharyngolaryngeal inflammatory pathology followed by a professional dysphonia. They insisted upon the use of the standard including questionnaire and the methods of investigation for the vocal parameters during the gastroesophageal reflux disease (GERD). Alcohol intake, smoking, stress, gastro esophageal reflux and different allergens influenced the symptoms and the objective alterations of the vocal performance (Reflux Finding Score - RFI, Reflux Symptoms Index - RSI). The symptoms, statistically significant, on the studied series were hoarseness (p=0.0l), postnasal drip (p=0.03), globus hystericus (p=0.0l). ENT examination revealed: erythema, congestion (p=0.0l), vocal folds edema (p=0.0l), profuse laryngeal edema (p=0.02), posterior commissure hypertrophy (p=0.02). Environmental factors and gastro esophageal reflux disease had a negative impact on the vocal performances of the vocal professionals. © 2012 IEEE.


Solomon S.,University of Medicine and Pharmacy of Iasi | Ursarescu I.,University of Medicine and Pharmacy of Iasi | Martu A.,University of Medicine and Pharmacy of Iasi | Luchian I.,University of Medicine and Pharmacy of Iasi | And 3 more authors.
Revista de Chimie | Year: 2015

The periodontal disease represents an inflammatory disease with an infectious etiology; the photo-activated disinfection of the periodontal pockets was proposed as an adjunctive form of treatment to the classic mechanical scaling and root planning. The purpose of our study is to assess the efficiency of the photo-activated toluidine blue on the clinical parameters of the patient with chronic periodontitis versus classic mechanical treatment alone.


PubMed | University of Medicine and Pharmacy of Iasi
Type: Journal Article | Journal: Journal of microbiology and biotechnology | Year: 2012

This paper is dedicated to the study on the external and internal mass transfers of glucose for succinic acid fermentation under substrate and product inhibitions using a bioreactor with stirred bed of immobilized Actinobacillus succinogenes cells. By means of the substrate mass balance for a single particle of biocatalysts, considering the kinetic model adapted for both inhibitory effects, specific mathematical models were developed for describing the profiles of the substrate concentration in the outer and inner regions of biocatalysts and for estimating the substrate mass flows in the liquid boundary layer surrounding the particle and inside the particle. The values of the mass flows were significantly influenced by the internal diffusion velocity and rate of the biochemical reaction of substrate consumption. These cumulated influences led to the appearance of a biological inactive region near the particle center, its magnitude varying from 0 to 5.3% of the overall volume of particles.


PubMed | University of Medicine and Pharmacy of Iasi and Fertility Reproductive Medical Center Omini Clinic Iasi
Type: Journal Article | Journal: Current health sciences journal | Year: 2014

The aim of the present study was to compare the GnRH agonist long protocol with the flexible GnRH antagonist protocol in infertile PCOS women undergoing COS in terms of clinical pregnancy rate (CPR), with special reference to the incidence of OHSS. Materials and Methods. The study was conducted at the Hospital Obstetrics and Gynecology Cuza Vod Iai and Fertility Reproductive Medical Center Omini Clinic Iai from June 1, 2010, to September 31, 2012. PCOS as defined by the Rotterdam 2003 consensus, i.e. presence of two of the following three features: presence of oligo- and/ or anovulation, clinical and/or biochemical signs of hyperandrogenism, polycystic ovaries and exclusion of other endocrinopathies. Results. No differences were observed in clinical pregnancy rate (CPR) in the agonist and antagonist protocols, respectively. Incidence of OHSS was lower in the antagonist compared with agonist group (4% versus 28%). Duration of stimulation (13,80 + 1,4 vs 11,85 + 2,4 p < 0,001) and total gonadotrophin required (2435,5 + 884,5 versus 2005, 5 + 545,5 IU p < 0.003) were also lower in the antagonist compared with agonist protocol. Conclusions. The current study suggests that the flexible GnRH antagonist protocol is associated with a similar ongoing pregnancy rate, lower incidence of OHSS grade II, lower gonadotrophin requirement and shorter duration of stimulation, compared with GnRH agonist. The GnRH antagonist might be the treatment choice for patients with PCOS undergoing IVF.

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