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Bolfa P.,University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca | Catoi C.,University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca | Gal A.,University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca | Taulescu M.,University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca | And 6 more authors.
Romanian Biotechnological Letters

Classical medicinal treatments tend to be replaced by alternative therapies, in which the natural plant extracts are being used. In the current study we studied the effects of five alcoholic plant extracts (Calendula flos, Echinacea pallida, Echinacea purpurea, Urtica dioica and Aloe vera) on the dynamics of some immune effectors of equine infectious anemia virus (EIAV) infected horses in vitro. The research focused on the effects of plants active principles on the cellular non-specific defence subsystem and on the functional capacity of the T lymphocyte subsystem. Our findings suggest that the stimulatory effect of Urtica dioica alcoholic extract on the phagocytic function in young, recently EIAV infected horses is followed by an inhibitory effect, that could be explained by the possible rapid carbon particle ingestion, and then by increase cell membrane fragility. Although very closed related taxonomically, the two Echinacea extracts studied here don't share the same effect in vitro, meaning that different principles are responsible for their in vitro effects. Young horses recently infected with EIAV have an overall increased cellular reactivity compared to older animals, due to infection. On the other hand, due to general increased immunoreactivity in younger animals, the immune recovery effects of the used alcoholic extracts is marked in younger equines. © 2011 University of Bucharest. Source

Sovrea A.,University of Medicine and Farmacy Iuliu Hatieganu | Vornicescu C.,University of Medicine and Farmacy Iuliu Hatieganu | Zerbea M.I.,University of Medicine and Farmacy Iuliu Hatieganu | Yacoob S.,University of Medicine and Farmacy Iuliu Hatieganu | Stan N.D.,University of Medicine and Farmacy Iuliu Hatieganu
Annals of the Romanian Society for Cell Biology

Immunohistochemistry is complementary to the histological diagnosis (by optical and electronical microscopy) related with the clinical-pathology data. Though, there are cases when the interaction of specific antibodies with proteins located at the tumor site, is the only method that offers suitable information for a final diagnosis. The aim of this study is to determine a correlation between the intensity of the immunohistochemical staining and the malignancy degree. Twenty cases of gliomas, diagnosed at the Emergency County Hospital in Cluj-Napoca, Neurosurgery Clinic between 2007 - 2009 were studied. They were graded according to the World Health Organization/2007 grading system. For staining, the following monoclonal antibodies Mouse antihuman were used: Actin - clone 1A 4 DAKO and GFAP (glial fibrillary acidic protein) - clone GF 2 DAKO. Using the anti-GFAP staining and the ImmunoMarking Intensity Score (IMIS) a statistic study was carried out regarding the possible correlations between the number of cells, the intensity of the staining, and the malignancy degree. The data was analysed using T-test. Data having p-value < 0.05 was considered statistically relevant. The actin antibody illustrates a raise in the number of sanguine vessels in low-differentiated tumors compared to high-differentiated ones. High cellularity of malignant tumors requires increased oxygen intake, leading to angiogenesis, sustained by the presence of actin. The GFAP antibody determines a positive cytoplasmatic response in all studied cases, but having different intensities. The GFAP stained slides showed that in low-differentiated astrocytomas the number of clearly marked cells is diminished (p<0.05) and there is a high amount of unstained cells (p<0.05). Being a marker of astrocytic differentiation, its absence is associated with a higher degree of malignancy. Thus, the more anaplastic the tumor is, the less GFAP exist in the cells. Consequently the GFAP immunostaining allows an appropriate diagnosis and facilitates a targeted therapeutic attitude. Source

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