University of Medicine and Farmacy Iuliu Hatieganu

Cluj-Napoca, Romania

University of Medicine and Farmacy Iuliu Hatieganu

Cluj-Napoca, Romania
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Gulei D.,University of Medicine and Farmacy Iuliu Hatieganu | Mehterov N.,Medical university-Plovdiv | Mehterov N.,Technological Center for Emergency Medicine | Ling H.,University of Houston | And 5 more authors.
Biochimica et Biophysica Acta - General Subjects | Year: 2017

Background: Lack of early diagnosis methods and the development of drug resistance are among the main reasons for increased mortality rates within breast cancer patients. These two aspects are governed by specific pro-carcinogenic modifications, where TGBβ-induced EMT is one of the leading actors. Endowment of the epithelial cells with mesenchymal characteristics allows them to migrate and invade secondary tissues in order to form malignant sites and also confers chemoresistance. TGFβ which role switches from the tumor suppressor cytokine to the oncogenic one favoring the tumor microenvironment regulates this process. Scope of review: This review aims to comprehensively present the updated TGFβ-induced EMT in breast cancer, including the regulatory role of the non-coding RNAs with focus on the miR-200 family and newly discovered lncRNAs such as HOTAIRM1. Additionally, a new phenotype, P-EMT, also modulated by miR-200 and miR-34 families that form complex feedback loops with TGFβ, SNAI1 and ZEB1/2 is presented under an updated form. Major conclusions: The hallmarks of EMT are becoming increasingly associated with aggressive forms of breast cancer and low survival rates among patients. Considering that this phenotypical switch can trigger drug resistance, invasion and metastasis, inhibition of EMT could represent an important milestone in mammary cancer treatment. General significance: The present review assembles the most recent data regarding TGFβ induced EMT, including the input of non-coding RNAs, contributing to the possible development of new targeted treatment strategies for cancer patients. © 2017 Elsevier B.V.

Braicu C.,University of Medicine and Pharmacy, Cluj-Napoca | Braicu C.,The Oncological Institute Prof Dr Ion Chiricuta | Cojocneanu-Petric R.,University of Medicine and Pharmacy, Cluj-Napoca | Jurj A.,University of Medicine and Pharmacy, Cluj-Napoca | And 7 more authors.
BMC Genomics | Year: 2016

Background: Zearalenone (ZEA) is a secondary metabolite produced by Fusarium species. ZEA was proved to exert a wide range of unwanted side effects, but its mechanism of action, particularly at duodenum levels, remains unclear. In our study based on the microarray technology we assessed the alteration of gene expression pattern Sus scrofa duodenum which has been previously exposed to ZEA. Gene expression data was validated by qRT-PCR and ELISA. The gene expression data were further extrapolated the results to their human orthologues and analyzed the data in the context of human health using IPA (Ingenuity Pathways Analysis). Results: Using Agilent microarray technology, we found that gene expression pattern was significantly affected by ZEA exposure, considering a 2-fold expression difference as a cut-off level and a p-value < 0.05. In total, we found 1576 upregulated and 2446 downregulated transcripts. About 1084 genes (764 downregulated and 751 overexpressed) were extrapolated to their human orthologues. IPA analysis showed various altered key cellular and molecular pathways. As expected, we observed a significant alteration of immune response related genes, MAPK (mitogen activate protein kinases) pathways or Toll-Like Receptors (TLRs). What captured our attention was the modulation of pathways related to the activation of early carcinogenesis. Conclusions: Our data demonstrate that ZEA has a complex effect at duodenum level. ZEA is able to activate not only the immune response related genes, but also those relate to colorectal carcinogenesis. The effects can be more dramatic when connected with the exposure to other environmental toxic agents or co-occurrence with different microorganisms. © 2016 The Author(s).

Sovrea A.,University of Medicine and Farmacy Iuliu Hatieganu | Vornicescu C.,University of Medicine and Farmacy Iuliu Hatieganu | Zerbea M.I.,University of Medicine and Farmacy Iuliu Hatieganu | Yacoob S.,University of Medicine and Farmacy Iuliu Hatieganu | Stan N.D.,University of Medicine and Farmacy Iuliu Hatieganu
Annals of the Romanian Society for Cell Biology | Year: 2011

Immunohistochemistry is complementary to the histological diagnosis (by optical and electronical microscopy) related with the clinical-pathology data. Though, there are cases when the interaction of specific antibodies with proteins located at the tumor site, is the only method that offers suitable information for a final diagnosis. The aim of this study is to determine a correlation between the intensity of the immunohistochemical staining and the malignancy degree. Twenty cases of gliomas, diagnosed at the Emergency County Hospital in Cluj-Napoca, Neurosurgery Clinic between 2007 - 2009 were studied. They were graded according to the World Health Organization/2007 grading system. For staining, the following monoclonal antibodies Mouse antihuman were used: Actin - clone 1A 4 DAKO and GFAP (glial fibrillary acidic protein) - clone GF 2 DAKO. Using the anti-GFAP staining and the ImmunoMarking Intensity Score (IMIS) a statistic study was carried out regarding the possible correlations between the number of cells, the intensity of the staining, and the malignancy degree. The data was analysed using T-test. Data having p-value < 0.05 was considered statistically relevant. The actin antibody illustrates a raise in the number of sanguine vessels in low-differentiated tumors compared to high-differentiated ones. High cellularity of malignant tumors requires increased oxygen intake, leading to angiogenesis, sustained by the presence of actin. The GFAP antibody determines a positive cytoplasmatic response in all studied cases, but having different intensities. The GFAP stained slides showed that in low-differentiated astrocytomas the number of clearly marked cells is diminished (p<0.05) and there is a high amount of unstained cells (p<0.05). Being a marker of astrocytic differentiation, its absence is associated with a higher degree of malignancy. Thus, the more anaplastic the tumor is, the less GFAP exist in the cells. Consequently the GFAP immunostaining allows an appropriate diagnosis and facilitates a targeted therapeutic attitude.

Bolfa P.,University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca | Catoi C.,University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca | Gal A.,University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca | Taulescu M.,University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca | And 6 more authors.
Romanian Biotechnological Letters | Year: 2011

Classical medicinal treatments tend to be replaced by alternative therapies, in which the natural plant extracts are being used. In the current study we studied the effects of five alcoholic plant extracts (Calendula flos, Echinacea pallida, Echinacea purpurea, Urtica dioica and Aloe vera) on the dynamics of some immune effectors of equine infectious anemia virus (EIAV) infected horses in vitro. The research focused on the effects of plants active principles on the cellular non-specific defence subsystem and on the functional capacity of the T lymphocyte subsystem. Our findings suggest that the stimulatory effect of Urtica dioica alcoholic extract on the phagocytic function in young, recently EIAV infected horses is followed by an inhibitory effect, that could be explained by the possible rapid carbon particle ingestion, and then by increase cell membrane fragility. Although very closed related taxonomically, the two Echinacea extracts studied here don't share the same effect in vitro, meaning that different principles are responsible for their in vitro effects. Young horses recently infected with EIAV have an overall increased cellular reactivity compared to older animals, due to infection. On the other hand, due to general increased immunoreactivity in younger animals, the immune recovery effects of the used alcoholic extracts is marked in younger equines. © 2011 University of Bucharest.

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