Mazur L.,Maria Curie Sklodowska University |
Modzelewska-Banachiewicz B.,Nicolaus Copernicus University |
Paprocka R.,Nicolaus Copernicus University |
Zimecki M.,Polish Academy of Sciences |
And 4 more authors.
Journal of Inorganic Biochemistry | Year: 2012
A new linear amidrazone derivative, 6-acetyl-cyclohex-3-enecarboxylic acid [1-pyridin-2-yl-1-(pyridyn-2-yloamin)meth-(Z)-ylidene] hydrazide, H2L (2) and its Cu(II) complex, [Cu2L2]·4H 2O (3) were synthesized and characterized by elemental analysis, IR and 1H NMR spectroscopy and cyclic voltammetry. Compound 2 was synthesized in the equimolar reaction of N3-substituted amidrazone with cis-1,2,3,6-tetrahydrophthalic anhydride. The Cu complex of 2 was obtained in the reaction with copper(II) acetate. The molecular structures of 2 and 3 were determined by X-ray crystallography. The parent ligand exists in its amide-hydrazone form in the solid state. The central amidrazone moiety has a Z configuration with respect to the double CN bond. Coordination to the metal center promotes Z/E isomerization of the hydrazone group of the ligand. Compound 3 is a dinuclear four-coordinated Cu(II) complex with the amidrazone ligand behaving as a tetradentate double deprotonated chelating one. Several biological activities of 2 and 3 were examined in vitro; they were: antimicrobial properties against selected bacterial and fungal strains, suppression of phytohemagglutinin A (PHA)-induced proliferation of human peripheral blood mononuclear cells (PBMC) and their effects on tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) production. The cytotoxic activity of Cu(II) complex was determined with respect to the four carcinoma cell lines (SW 984, CX-1, L-1210, A-431). The studied complex exhibited significant cytotoxic effects (particularly against CX-1 colon carcinoma), comparable to those reported for cisplatin. Both compounds have shown a relatively low antibacterial activity and were devoid of antifungal properties. © 2012 Elsevier Inc. All rights reserved.
Wegiera M.,Medical University of Lublin |
Grabarczyk P.,Medical University of Lublin |
Baraniak B.,Agricultural University of Lublin |
Smolarz H.D.,Medical University of Lublin
Acta Biologica Cracoviensia Series Botanica | Year: 2011
We used the DPPH method to assess in vitro the antiradical activity of extracts from the roots, leaves and fruits of six Rumex L. (dock) species. Data from preliminary screening indicated that all the tested extracts showed antioxidant properties. The degree of antiradical activity depended upon the plant part. Fruit extracts from R. hydrolapathum Huds., R. obtusifolius L. and R. confertus Willd. showed stronger antiradical properties than the other tested material. We also determined tannin content levels in the extracts and their correlation with antioxidant activity. © Polish Academy of Sciences and Jagiellonian University, Cracow 2011.