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Bucar Pajek M.,University of LjubljanaLjubljana | Leskosek B.,University of LjubljanaLjubljana | Vivoda T.,University of LjubljanaLjubljana | Svilan K.,University of LjubljanaLjubljana | And 2 more authors.
Therapeutic Apheresis and Dialysis | Year: 2016

To reduce the need for a large number of executed physical function tests we examined inter-relations and determined predictive power for daily physical activity of the following tests: 6-min walk, 10 repetition sit-to-stand, time up-and-go, Storke balance, handgrip strength, upper limb tapping and sitting forward bend tests. In 90 dialysis and 140 healthy control subjects we found high correlations between all tests, especially those engaging lower extremities. Sit-to-stand, forward bend and handgrip strength were selected for the test battery and composite motor performance score. Sit-to-stand test was superior in terms of sensitivity to uremia effects and association with daily physical function in adjusted analyses. There was no incremental value in calculating the composite performance score. We propose to standardize the physical function assessment of dialysis patients for cross-sectional and longitudinal observations with three simple, cheap, well-accessible and easily performed test tools: sit-to-stand test, handgrip strength and Human Activity Profile questionnaire. © 2016 International Society for Apheresis, Japanese Society for Apheresis, and Japanese Society for Dialysis Therapy Source

Maneek-Keber M.,National Institute of ChemistryLjubljana | Frank-Bertoncelj M.,University Medical Center LjubljanaLjubljana | Hafner-Bratkovie I.,National Institute of ChemistryLjubljana | Smole A.,National Institute of ChemistryLjubljana | And 9 more authors.
Science Signaling | Year: 2015

Oxidative stress produced in response to infection or sterile injury activates the innate immune response. We found that extracellular vesicles (EVs) isolated from the plasma of patients with rheumatoid arthritis or secreted from cells subjected to oxidative stress contained oxidized phospholipids that stimulated cells expressing Toll-like receptor 4 (TLR4) in a manner dependent on its co-receptor MD-2. EVs from healthy subjects or reconstituted synthetic EVs subjected to limited oxidation gained the ability to stimulate TLR4-expressing cells, whereas prolonged oxidation abrogated this property. Furthermore, we found that 15-lipoxygenase generated hydro(pero)xylated phospholipids that stimulated TLR4-expressing cells. Molecular modeling suggested that the mechanism of activation of TLR4 by oxidized phospholipids in EVs was structurally similar to that of the TLR4 ligand lipopolysaccharide (LPS). This was supported by experiments showing that EV-mediated stimulation of cells required MD-2, that mutations that block LPS binding to TLR4 abrogated the stimulatory effect of EVs, and that EVs induced TLR4 dimerization. On the other hand, analysis of gene expression profiles showed that genes encoding factors that resolve inflammation were more abundantly expressed in responses to EVs than in response to LPS. Together, these data suggest that EVs act as an oxidative stress-induced endogenous danger signal that underlies the pervasive role of TLR4 in inflammatory diseases. Source

Budic-leto I.,Institute for Adriatic Crops | Humar I.,Institute for Adriatic Crops | Zdunic G.,Institute for Adriatic Crops | Hribar J.,University of LjubljanaLjubljana | Zlatic E.,University of LjubljanaLjubljana
Czech Journal of Food Sciences | Year: 2015

Prošek dessert wines produced from dried grapes of two native Croatian varieties, one white and one red, had complex volatile compositions. Various categories of volatile compounds were identified using headspace solid-phase microextraction (HS-SPME) coupled to gas chromatography-mass spectrometry (GC-MS). Sixty-one individual volatile compounds of Prošek were identified. Of these, nine compounds comprised over 95% of the total peak area of volatile aromas detected in Prošek: five esters and four alcohols. The remaining volatile compounds consisted of 11 alcohols, 11 esters, 10 terpenes, 8 aldehydes, 10 ketones, 4 acids, 1 lactone, and 1 norisoprenoide. Only 13 of the 61 aroma compounds showed significant differences between the varieties investigated. These results suggest that the characteristic aroma of Prošek is very complex and determined predominantly by the unique process of the grape drying. Source

Kandus A.,University Medical Center LjubljanaLjubljana | Buturovic Ponikvar J.,University Medical Center LjubljanaLjubljana | Buturovic Ponikvar J.,University of LjubljanaLjubljana | Mlinsek G.,University Medical Center LjubljanaLjubljana | And 3 more authors.
Therapeutic Apheresis and Dialysis | Year: 2016

This report gives an overview of kidney transplantation in Slovenia, a country with a population of 2.1 million and one transplant center. The establishment of a national transplant organization resulted in the acceptance of Slovenia into Eurotransplant (ET) in 2000. Between 1970 and 2015, 1158 kidney transplantations were performed. From 1970 to 2009, 126 patients were transplanted from living related donors, only two in the ET period. From 1986 to 1999, 239 patients received kidney grafts from deceased donors, while 793 patients were transplanted from deceased donors after joining ET. In ET period, 1- and 5-year patient survival rates were 98.1% and 93.8%, and the concomitant graft survival rates were 94.3% and 87.5%, respectively. During the ET period, the number of deceased donor kidney transplants per year was three times higher than in the 14 years before. Patient and graft survival rates have been very good and entirely comparable to those in large reports. © 2016 International Society for Apheresis, Japanese Society for Apheresis, and Japanese Society for Dialysis Therapy Source

Zhao Y.X.,Hefei University of Technology | Allada K.,Massachusetts Institute of Technology | Allada K.,Jefferson Lab | Aniol K.,California State University, Los Angeles | And 126 more authors.
Physical Review C - Nuclear Physics | Year: 2015

We report the measurement of beam-target double spin asymmetries (A LT) in the inclusive production of identified hadrons, e + 3 He → h + X, using a longitudinally polarized 5.9-GeV electron beam and a transversely polarized He 3 target. Hadrons (π ±, K ±, and proton) were detected at 16 with an average momentum P h = 2.35 GeV/c and a transverse momentum (p T) coverage from 0.60 to 0.68 GeV/c. Asymmetries from the He 3 target were observed to be nonzero for π ± production when the target was polarized transversely in the horizontal plane. The π + and π - asymmetries have opposite signs, analogous to the behavior of A LT in semi-inclusive deep-inelastic scattering. © 2015 American Physical Society. Source

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