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Póvoa e Meadas, Portugal

Laranjo L.,University Nova Of Lisboalisboa | Neves A.L.,University of Porto | Costa A.,APDP | Ribeiro R.T.,APDP | And 2 more authors.
European Journal of General Practice | Year: 2015

Background: Patients with type 2 diabetes mellitus (DM) have a central role in managing their disease, but the effective adoption of self-management behaviours is often challenging. Objectives: The main objective of this study was to assess the facilitators, barriers and expectations in the self-management of type 2 DM, as perceived by patients. Methods: Patients with type 2 DM were recruited at the Portuguese Diabetes Association outpatient clinic, using a convenient sampling technique. Qualitative data was obtained using video-recorded focus groups. Each session had a moderator and an observer, and followed a pre-tested questioning route. Two independent researchers transcribed and analysed the focus groups. Results: Three major themes were identified: diet, physical exercise, and glycaemic control. Difficulties in changing dietary habits were grouped in four main categories: decisional, food quality, food quantity, and dietary schedule. Barriers related to physical exercise also included decisional aspects, as well as fatigue, muscle and joint pain, and other co-morbidities. Information and knowledge translation, as well as family and social ties, were commonly explored aspects across the three themes and were regarded as facilitators in some situations and as barriers in others. Conclusion: This study provided new insight on the barriers, facilitators and expectations in type 2 DM self-management, pointing out the importance of tailored guidance. Future research should explore interventions designed to promote and facilitate behaviour change in this population. © 2015 Informa Healthcare.

Correia C.,New University of Lisbon | Correia C.,Institute Biologia Experimental e Tecnologica | Koshkin A.,New University of Lisbon | Koshkin A.,Institute Biologia Experimental e Tecnologica | And 10 more authors.
Stem Cells Translational Medicine | Year: 2016

To fully explore the potential of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs), efficient methods for storage and shipment of these cells are required. Here, we evaluated the feasibility to cold store monolayers and aggregates of functional CMs obtained from different PSC lines using a fully defined clinical-compatible preservation formulation and investigated the time frame that hPSC-CMs could be subjected to hypothermic storage.We showed that two-dimensional (2D) monolayers of hPSC-CMs can be efficiently stored at 4°C for 3 days without compromising cell viability. However, cell viability decreasedwhen the cold storage intervalwas extended to 7 days.We demonstrated that hPSC-CMs are more resistant to prolonged hypothermic storage-induced cell injury in three-dimensional aggregates than in 2D monolayers, showing high cell recoveries (>70%) after 7 days of storage. Importantly, hPSC-CMs maintained their typical (ultra)structure, gene and protein expression profile, electrophysiological profiles, and drug responsiveness. © AlphaMed Press 2016.

Gomis-Berenguer A.,CSIC - National Coal Institute | Iniesta J.,University of Alicante | Moro A.,University Nova Of Lisboalisboa | Maurino V.,University of Turin | And 2 more authors.
Carbon | Year: 2016

We showed the effect of commensurate confinement in the pores of nanoporous carbons in the conversion of visible light into a chemical reaction. By using a series of nanoporous carbons with a controlled distribution of pore sizes obtained from a gradual activation under moderate conditions, we have demonstrated the superior conversion of light in the constrained pore space of the carbons compared to values in solution. Besides a more efficient conversion of light, nanopore confinement resulted in a 100-200 nm redshift in the wavelength onset of the photochemical reaction. The visible light activity was boosted in pores which sizes match the dimensions of the confined compound. We attribute this to the enhanced splitting and charge separation of the photogenerated species in the nanopores, due to the proximity of the charge carriers and the adsorbed molecules. © 2015 Elsevier Ltd.

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