University of KentuckyKentucky

University, United States

University of KentuckyKentucky

University, United States

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Pena F.J.,University of Veterinary and Animal Sciences | Ball B.A.,University of KentuckyKentucky | Squires E.L.,University of KentuckyKentucky
Cytometry Part B - Clinical Cytometry | Year: 2017

Multiparametric assessment of stallion sperm quality using flow cytometry can be a useful adjunct in semen evaluation; however, the availability of flow cytometers in veterinary practice is limited. The ability to preserve and transport sperm samples for later flow cytometric analysis using fixable probes would potentially facilitate this process. In the current study, we validated the combination of live/dead Zombie Green® (a fixable dye used to assess live and dead sperm) and MitoTracker Deep Red® (used to assess mitochondrial membrane potential). The assay was validated against classic, non-fixable, membrane assays (SYBR-14/PI). Our results demonstrated the feasibility of the assay. In conclusion, stained and fixed semen samples stored for 72 h obtained equivalent results to the exam on the same day; this new protocol shall facilitate the wider use of flow cytometry in stallion andrology in the future. © 2016 International Clinical Cytometry Society.


Gao X.,University of KentuckyKentucky | Omosebi A.,University of KentuckyKentucky | Landon J.,University of KentuckyKentucky | Liu K.,University of KentuckyKentucky
Journal of the Electrochemical Society | Year: 2014

In order to examine the reliability of carbon xerogel (CX) materials for capacitive deionization (CDI), long-term operation of a CDI cell with CX electrodes was performed in a dilute NaCl solution. During operation at 1.2/0 V, the deionization performance gradually degraded, and a growing inversion peak formed at the beginning of each adsorption step. When the polarity of the electrode was reversed, the use of -1.2/0 V significantly boosted deionization performance. However, this enhancement was not sustained with continued cycling. In this study, we also reported the use of a four-electrode cell, in which the potential distribution for a pair of electrodes could be measured, and the potential of zero charge (PZC) for a single electrode could be located. Based upon the PZC location for the used electrodes with respect to the corresponding potential distribution, performance variation and the formation of the inversion peak for long-term CDI operation were interpreted. In addition, N2 adsorption-desorption measurements and surface morphology examinations indicated that there was little variation in the porosity of the cycled electrodes with long-term operation. © 2014 The Electrochemical Society. All rights reserved.

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