Tsuge M.,National Chiao Tung University |
Kalinowski J.,University of Helsinki |
Gerber R.B.,University of Helsinki |
Gerber R.B.,University of Jerusalem |
And 2 more authors.
Journal of Physical Chemistry A | Year: 2015
Proton-bound rare-gas dimer (RgHRg)+, in which Rg represents a rare-gas atom, serves as a prototypical system for proton solvation by inert-gas atoms. Until now, only centrosymmetric species with Rg = Ar, Kr, or Xe have been identified with infrared spectra. We employed electron bombardment during deposition of a mixture of Xe (or Kr) in p-H2 at 3.2 K to prepare (RgHRg)+. Lines at 847.0 and 972.1 cm-1 are assigned as the Rg-H-Rg antisymmetric stretching (μ3) mode and its combination with the Rg-H-Rg symmetric stretching (μ1 + μ3) mode of (XeHXe)+ in solid p-H2, respectively. Lines at 871.1 and 974.0 cm-1 are assigned as the μ3 and μ1 + μ3 modes of (KrHKr)+ in solid p-H2, respectively. Slightly shifted and broadened lines were observed for these species in solid n-H2. These results agree satisfactorily with reported experimental values of (XeHXe)+ and (KrHKr)+ in solid Xe, Kr, and Ar, and with the quantum-chemically predicted anharmonic vibrational wavenumbers of these species in the gaseous phase; the significant spectral shifts in various matrixes are rationalized with the proton affinities of the hosts. When a mixture of Xe and Kr in p-H2 was used, an additional broad feature at 1284 cm-1 was observed and assigned as the μ3 mode of (KrHXe)+ in solid p-H2. This line shifted to 1280 cm-1 in solid n-H2 and the corresponding line of (KrDXe)+ was observed at 954 cm-1 in n-D2. The observations of these lines are new; the wavenumbers significantly blue shifted from those of the centrosymmetric (RgHRg)+ agree with the quantum-chemically predicted anharmonic vibrational wavenumbers of 1279 cm-1 for (KrHXe)+ and 916 cm-1 for (KrDXe)+. Analysis of the computational results shows that electronic correlation effects play a much greater role for the asymmetric than for the symmetric species. An interpretation for this is provided. © 2014 American Chemical Society.
Mathias A.,Cardiology Division |
Moss A.J.,Cardiology Division |
Lopes C.M.,University of Rochester |
Barsheshet A.,Cardiology Division |
And 14 more authors.
Heart Rhythm | Year: 2013
Background: Individual corrected QT interval (QTc) may vary widely among carriers of the same long QT syndrome (LQTS) mutation. Currently, neither the mechanism nor the implications of this variable penetrance are well understood. Objectives: To hypothesize that the assessment of QTc variance in patients with congenital LQTS who carry the same mutation provides incremental prognostic information on the patient-specific QTc. Methods: The study population comprised 1206 patients with LQTS with 95 different mutations and≥5 individuals who carry the same mutation. Multivariate Cox proportional hazards regression analysis was used to assess the effect of mutation-specific standard deviation of QTc (QTcSD) on the risk of cardiac events (comprising syncope, aborted cardiac arrest, and sudden cardiac death) from birth through age 40 years in the total population and by genotype. Results: Assessment of mutation-specific QTcSD showed large differences among carriers of the same mutations (median QTcSD 45 ms). Multivariate analysis showed that each 20 ms increment in QTcSD was associated with a significant 33% (P =.002) increase in the risk of cardiac events after adjustment for the patient-specific QTc duration and the family effect on QTc. The risk associated with QTcSD was pronounced among patients with long QT syndrome type 1 (hazard ratio 1.55 per 20 ms increment; P<.001), whereas among patients with long QT syndrome type 2, the risk associated with QTcSD was not statistically significant (hazard ratio 0.99; P =.95; P value for QTcSD-by-genotype interaction =.002). Conclusions: Our findings suggest that mutations with a wider variation in QTc duration are associated with increased risk of cardiac events. These findings appear to be genotype-specific, with a pronounced effect among patients with the long QT syndrome type 1 genotype. © 2013 Heart Rhythm Society.
PubMed | University of Pavia, Papa Giovanni XXIII Hospital, University of Jerusalem, Maggiore Parma Hospital and 10 more.
Type: | Journal: World journal of emergency surgery : WJES | Year: 2015
Lesions of the upper digestive tract due to ingestion of caustic agents still represent a major medical and surgical emergency worldwide. The work-up of these patients is poorly defined and no clear therapeutic guidelines are available.The aim of this study was to provide an evidence-based international consensus on primary and secondary prevention, diagnosis, staging, and treatment of this life-threatening and potentially disabling condition.An extensive literature search was performed by an international panel of experts under the auspices of the World Society of Emergency Surgery (WSES). The level of evidence of the screened publications was graded using the Oxford 2011 criteria. The level of evidence of the literature and the main topics regarding foregut caustic injuries were discussed during a dedicated meeting in Milan, Italy (April 2015), and during the 3rd Annual Congress of the World Society of Emergency Surgery in Jerusalem, Israel (July 2015).One-hundred-forty-seven full papers which addressed the relevant clinical questions of the research were admitted to the consensus conference. There was an unanimous consensus on the fact that the current literature on foregut caustic injuries lacks homogeneous classification systems and prospective methodology. Moreover, the non-standardized definition of technical and clinical success precludes any accurate comparison of therapeutic modalities. Key recommendations and algorithms based on expert opinions, retrospective studies and literature reviews were proposed and approved during the final consensus conference. The clinical practice guidelines resulting from the consensus conference were approved by the WSES council.The recommendations emerging from this consensus conference, although based on a low level of evidence, have important clinical implications. A world registry of foregut caustic injuries could be useful to collect a homogeneous data-base for prospective clinical studies that may help improving the current clinical practice guidelines.
Liu J.F.,Heart Research Follow up Program |
Moss A.J.,Heart Research Follow up Program |
Jons C.,Heart Research Follow up Program |
Benhorin J.,University of Jerusalem |
And 10 more authors.
American Journal of Cardiology | Year: 2010
The clinical course of patients with 2 relatively common long QT syndrome type 3 mutations has not been well described. In the present study, we investigated the mutational-specific risk in patients with deletional (ΔKPQ) and missense (D1790G) mutations involving the SCN5A gene. The study population involved 50 patients with the ΔKPQ mutation and 35 patients with the D1790G mutation. The cumulative probability of a first cardiac event (syncope, aborted cardiac arrest, or long QT syndrome-related sudden death) was evaluated using the Kaplan-Meier method. The Cox proportional hazards survivorship model was used to determine the independent contribution of clinical and genetic factors to the first occurrence of cardiac events from birth through 40 years of age. The Andersen-Gill proportional intensity regression model was used to analyze the factors associated with recurrent syncope. Patients with a ΔKPQ mutation had a significantly greater probability of a first cardiac event from birth through 40 years of age (34%) than those with the D1790G mutation (20%; p <0.001). Multivariate analysis demonstrated an increased risk of cardiac events among ΔKPQ carriers compared to D1790G carriers (hazard ratio 2.42, p <0.0001) after adjustment for gender and QTc duration. Patients with ΔKPQ mutations also had an increased risk of recurrent syncope (hazard ratio 5.20, p <0.001). In conclusion, the clinical course of patients with long QT syndrome type with ΔKPQ mutations was shown to be more virulent than those with D1790G mutations, and this effect was independent of QTc duration. The findings highlight the importance of knowing the specific mutation in risk stratification of patients with long QT syndrome type 3. © 2010 Elsevier Inc. All rights reserved.
Cohen G.,University of Jerusalem |
Lecht S.,University of Jerusalem |
Oron-Herman M.,Advanced Technology Center |
Momic T.,University of Jerusalem |
And 2 more authors.
International Journal of Molecular Sciences | Year: 2013
In this study, we present the applicability of imaging epidermal growth factor (EGF) receptor levels in preclinical models of COLO205 carcinoma cells in vitro, mice with orthotopic tumors and ex vivo colorectal tumor biopsies, using EGF-labeled with IRDye800CW (EGF-NIR). The near infrared (NIR) bio-imaging of COLO205 cultures indicated specific and selective binding, reflecting EGF receptors levels. In vivo imaging of tumors in mice showed that the highest signal/background ratio between tumor and adjacent tissue was achieved 48 hours post-injection. Dissected colorectal cancer tissues from different patients demonstrated ex vivo specific imaging using the NIR bio-imaging platform of the heterogeneous distributed EGF receptors. Moreover, in the adjacent gastrointestinal tissue of the same patients, which by Western blotting was demonstrated as EGF receptor negative, no labeling with EGF-NIR probe was detected. Present results support the concept of tumor imaging by measuring EGF receptor levels using EGF-NIR probe. This platform is advantageous for EGF receptor bio-imaging of the NCI-60 recommended panel of tumor cell lines including 6-9 colorectal cell lines, since it avoids radioactive probes and is appropriate for use in the clinical setting using NIR technologies in a real-time manner. © 2013 by the authors; licensee MDPI, Basel, Switzerland.
Elkind Y.,University of Jerusalem |
Glick E.,University of Jerusalem |
Jacobson Y.,Zeraim Gedera |
Schor-Fumbarov T.,Zeraim Gedera
Acta Horticulturae | Year: 2014
The areas of pepper growing in passive protected cultivation regions with mild winter conditions are expanding. Simple plastic-covered structures facilitate protected pepper production during the winter. A typical crop is planted at the middle of the summer and ripe fruits are harvested during the fall, winter and spring seasons. Environmental conditions inside such structures are more extreme than those prevailing in climate-controlled greenhouses or outdoors. During the beginning of the production season when flowering and fruit set occur, the plants are exposed to extremely high day and night temperatures; these can be up to 45 and 25°C, respectively. In order to prevent even higher temperatures shading is applied. In the middle of the season, during the winter, minimum night temperatures are in the range 4-10°C, with infrequent frosts, and insufficient radiation. During this period growth continues and fruits are set, develop and ripen. It is proposed that cultivars which can thrive in such harsh conditions should be bred. Sufficient fruit set must occur under both high and low temperatures. The minimum night temperature for proper fruit set of blocky pepper was thought to be 18°C. Selection from a wide range of genetic variation under low night temperatures, led to the development of cultivars that can set normally shaped fruits at temperatures under 8-10°C. Those cultivars are also characterized with vigorous plants. Such cultivars fail to set under high temperatures during the beginning of the season. Special agrotechniques such as removal of shading, nitrogen starvation, and water stress seem to induce flower development and fruit set under high temperatures. This in turn extended the period of fruit setting, leading to a longer production season, and higher yields. It can be concluded that appropriate genetic advancement through breeding, combined with suitable agrotechnical means, can provide farmers with sustainable production systems.
Bar-El Dadon S.,University of Jerusalem |
Pascual C.Y.,Hospital Infantil La Paz |
Eshel D.,Israel Agricultural Research Organization |
Teper-Bamnolker P.,Israel Agricultural Research Organization |
And 2 more authors.
Food Chemistry | Year: 2013
IgE-mediated reactions to food allergens constitute a major health problem in industrialized countries. Chickpea is consumed in Mediterranean countries, and reportedly associated with IgE-mediated hypersensitivity reactions. However, the nature of allergic reactions to chickpea has not been characterized. A serum pool from paediatric patients allergic to chickpeas was used to detect IgE-binding proteins from chickpea seeds by immunoassay and immunoblot inhibition assay. Protein samples enriched in chickpea legumin and vicilin were obtained by anion exchange chromatography, and were identified by mass spectrometric analysis. IgE-immunoassays of globulin fractions from chickpeas revealed that vicilin (50 kDa) and the basic subunit of legumin (20 kDa) were bound by IgE from patient sera. Pea and lentil protein extracts strongly inhibited the IgE binding to chickpea globulin. We speculate that vicilin and the basic subunit of legumin are major chickpea allergens. Also, the globulin fraction of chickpea likely cross-reacts with the allergenic proteins of pea and lentil. © 2012 Elsevier Ltd. All rights reserved.
Lupicka M.,Polish Academy of Sciences |
Bodek G.,Polish Academy of Sciences |
Shpigel N.,University of Jerusalem |
Elnekave E.,University of Jerusalem |
Korzekwa A.J.,Polish Academy of Sciences
Reproduction | Year: 2015
The aim of this study was to identify uterine pluripotent cells both in bovine uterine tissues as well in epithelial, stromal, and myometrial uterine cell populations. Moreover, the relationship of pluripotent markers expression with age and the uterine horn side was considered. Uterine tissue was collected from ipsilateral and contralateral horns (days 8-10 of the estrous cycle). Immunohistostaining for C-KIT, OCT3/4, NANOG, and SOX2 in uterine tissue was determined. mRNA expression of C-KIT, OCT3/4, NANOG and SOX2 was evaluated in uterine tissue relative to the age of the cow and uterine horn side. Gene and protein expression of these markers in the uterine luminal epithelial, stromal, and myometrial cells was evaluated by real-time PCR and western blotting respectively. The expression of pluripotent cell markers OCT3/4, NANOG, and SOX2 was identified by flow cytometry assay in epithelial, stromal, and myometrial cells. Multilineage differentiation of the bovine uterine cells was performed. mRNA expression of OCT3/4, NANOG, and SOX2 in uterine tissue was higher in the ipsilateral horn than in the contralateral horn. Flow cytometry assay revealed positive fluorescence for OCT3/4, NANOG, and SOX2 in all uterine cell types. Results showed the age-dependent expression of pluripotent markers in uterine tissue. Beside, the different expression of pluripotent cells in each horn of uterus suggests the influence of ovarian hormones on these characteristics. The highest mRNA and protein expression for pluripotent markers was observed in stromal cells among uterine cells, which indicates this population of cells as the main site of pluripotent cells in the cow uterus. © 2015 Society for Reproduction and Fertility.
Rosenbaum D.,University of Jerusalem |
Zoran D.,University of Jerusalem |
Weiss Y.,University of Jerusalem
Advances in Neural Information Processing Systems | Year: 2013
Motivated by recent progress in natural image statistics, we use newly available datasets with ground truth optical flow to learn the local statistics of optical flow and compare the learned models to prior models assumed by computer vision researchers. We find that a Gaussian mixture model (GMM) with 64 components provides a significantly better model for local flow statistics when compared to commonly used models. We investigate the source of the GMM's success and show it is related to an explicit representation of flow boundaries. We also learn a model that jointly models the local intensity pattern and the local optical flow. In accordance with the assumptions often made in computer vision, the model learns that flow boundaries are more likely at intensity boundaries. However, when evaluated on a large dataset, this dependency is very weak and the benefit of conditioning flow estimation on the local intensity pattern is marginal.
Lichtenberg P.,University of Jerusalem
Psychiatric Quarterly | Year: 2011
Community homes for the treatment of the acutely psychiatrically ill have been established in several places in the world as alternatives to inpatient hospitalizations. We reviewed sources from the psychiatric and psychological literature which examine these models. Several features are common to this treatment setting: fewer residents in comparison with hospital wards; a supportive and caring milieu including intense and regular therapeutic contact with staff; a de-emphasis of medication; and a destigmatization of the therapeutic treatment of psychosis. The models differ with respect to use of psychotropic medication, emphasis upon first admissions, and composition of staff. The varying models are comparable to inpatient treatment in terms of clinical and psychosocial outcome, and generally cost less. For most patients requiring psychiatric hospitalization, residential treatment can be a viable alternative to inpatient hospitalization. Issues of cost, therapeutic effectiveness, and long-term outcomes still need to be studied. © 2011 Springer Science+Business Media, LLC.