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Asakawa T.,Dokkyo Medical University | Muramatsu A.,University of HyogoHyogo | Hayashi T.,Aino University | Urata T.,Osaka University | And 2 more authors.
Frontiers in Human Neuroscience | Year: 2014

The current study evaluated the effect of different anxiety states on information processing as measured by an electroencephalography (EEG) using emotional stimuli on a smartphone. Twenty-three healthy subjects were assessed for their anxiety states using The State Trait Anxiety Inventory (STAI) and divided into two groups: low anxiety (I, II) or high anxiety (III and IV, V). An EEG was performed while the participant was presented with emotionally laden audiovisual stimuli (resting, pleasant, and unpleasant sessions) and emotionally laden sentence stimuli (pleasant sentence, unpleasant sentence sessions) and EEG data was analyzed using propagation speed analysis. The propagation speed of the low anxiety group at the medial coronal for resting stimuli for all time segments was higher than those of high anxiety group. The low anxiety group propagation speeds at the medial sagittal for unpleasant stimuli in the 0–30 and 60–150 s time frames were higher than those of high anxiety group. The propagation speeds at 150 s for all stimuli in the low anxiety group were significantly higher than the correspondent propagation speeds of the high anxiety group. These events suggest that neural information processes concerning emotional stimuli differ based on current anxiety state. © 2014 Asakawa, Muramatsu, Hayashi, Urata, Taya and Mizuno-Matsumoto.

Daskalakis V.,Cyprus University of Technology | Ohta T.,University of HyogoHyogo | Kitagawa T.,University of HyogoHyogo | Varotsis C.,Cyprus University of Technology
Biochimica et Biophysica Acta - Bioenergetics | Year: 2015

Abstract Nitric oxide reductase (Nor) is the third of the four enzymes of bacterial denitrification responsible for the catalytic formation of laughing gas (N2O). Here we report the detection of the hyponitrite (HO-N = N-O-) species (νN-N = 1332 cm- 1) in the heme b3 Fe-FeB dinuclear center of Nor from Paracoccus denitrificans. We have also applied density functional theory (DFT) to characterize the bimetallic-bridging hyponitrite species in the reduction of NO to N2O by Nor and compare the present results with those recently reported for the N-N bond formation in the ba3 and caa3 oxidoreductases from Thermus thermophilus. © 2015 Published by Elsevier B.V.

Ueda H.,Kyoto University | Yokota E.,University of HyogoHyogo | Kuwata K.,Nagoya University | Kutsuna N.,University of Tokyo | And 9 more authors.
Plant Physiology | Year: 2016

The endoplasmic reticulum (ER) consists of dynamically changing tubules and cisternae. In animals and yeast, homotypic ER membrane fusion is mediated by fusogens (atlastin and Sey1p, respectively) that are membrane-associated dynamin-like GTPases. In Arabidopsis (Arabidopsis thaliana), another dynamin-like GTPase, ROOT HAIR DEFECTIVE3 (RHD3), has been proposed as an ER membrane fusogen, but direct evidence is lacking. Here, we show that RHD3 has an ER membrane fusion activity that is enhanced by phosphorylation of its C terminus. The ER network was RHD3-dependently reconstituted from the cytosol and microsome fraction of tobacco (Nicotiana tabacum) cultured cells by exogenously adding GTP, ATP, and F-actin. We next established an in vitro assay system of ER tubule formation with Arabidopsis ER vesicles, in which addition of GTP caused ER sac formation from the ER vesicles. Subsequent application of a shearing force to this system triggered the formation of tubules from the ER sacs in an RHD-dependent manner. Unexpectedly, in the absence of a shearing force, Ser/Thr kinase treatment triggered RHD3-dependent tubule formation. Mass spectrometry showed that RHD3 was phosphorylated at multiple Ser and Thr residues in the C terminus. An antibody against the RHD3 C-terminal peptide abolished kinase-triggered tubule formation. When the Ser cluster was deleted or when the Ser residues were replaced with Ala residues, kinase treatment had no effect on tubule formation. Kinase treatment induced the oligomerization of RHD3. Neither phosphorylation-dependent modulation of membrane fusion nor oligomerization has been reported for atlastin or Sey1p. Taken together, we propose that phosphorylation-stimulated oligomerization of RHD3 enhances ER membrane fusion to form the ER network. © 2016 American Society of Plant Biologists. All Rights Reserved.

Idota H.,Kinki University | Bunno T.,Kinki University | Tsuji M.,University of HyogoHyogo
Communications in Computer and Information Science | Year: 2015

A large number of firms use social media as new communication tools for obtaining information on consumer’s needs and market for developing new goods and services. Authors’ previous research found social media use promotes product innovation based on questionnaire data of Japanese firms. However, it was not clear whether social media effected directly on product innovation. This paper introduces a new variable constructed by achievement of product innovation and social media use and utilizes this as a dependent variable. The estimation methods conducted in the previous research are employed for probit analysis. This study finds all estimations have the same results. In addition, this paper also examines what kind of effect of social media is important for product innovation. The effects of social media such as obtaining trends and customer’s needs, and opening the FAQ site are found to be useful for achieving product innovation. © Springer-Verlag Berlin Heidelberg 2015.

Idota H.,Kinki University | Bunno T.,Kinki University | Ueki Y.,Institute of Development Economies | Komolavanij S.,Panyapiwat Institute of Management | And 2 more authors.
Communications in Computer and Information Science | Year: 2015

In order to achieve successful innovation, firms have to elevate their capability for innovation. ICT becomes one of essential tools of business management. The aim of this papers to examine the causal relationship between ICT use and innovation capability. Based on survey data from four ASEAN economies of Indonesia, the Philippines, Thailand, and Vietnam, this study examines what kind of ICT use such as SCM, CAD/CAM, SNS and so on, has been introduced by firms, and how it enhances internal innovation capability and contributes to achieve innovation. In so doing, to make the concept of internal innovation capability more tractable for empirical analysis, AHP (Analytical Hierarchy Process) was employed to construct an index of internal innovation capability. By using instrumental variable probit estimation (or two Stage Least Square: 2SLS), the following results are obtained: (1) ICT significantly influences to enhance internal innovation capability; and (2) internal innovation capability promotes innovation. © Springer-Verlag Berlin Heidelberg 2015.

Kimura Y.,University of Shizuoka | Kimura Y.,Tokyo Metropolitan Institute of Medical Science | Tanigawa M.,University of Tokyo | Kawawaki J.,Tokyo Metropolitan Institute of Medical Science | And 4 more authors.
Eukaryotic Cell | Year: 2015

Yeast Bro1 and Rim20 belong to a family of proteins which possess a common architecture of Bro1 and V domains. Alix and His domain protein tyrosine phosphatase (HD-PTP), mammalian Bro1 family proteins, bind YP(X)nL (n=1 to 3) motifs in their target proteins through their V domains. In Alix, the Phe residue, which is located in the hydrophobic groove of the V domain, is critical for binding to the YP(X)nL motif. Although the overall sequences are not highly conserved between mammalian and yeast V domains, we show that the conserved Phe residue in the yeast Bro1 V domain is important for binding to its YP(X)nLcontaining target protein, Rfu1. Furthermore, we show that Rim20 binds to its target protein Rim101 through the interaction between the V domain of Rim20 and the YPIKL motif of Rim101. The mutation of either the critical Phe residue in the Rim20 V domain or the YPIKL motif of Rim101 affected the Rim20-mediated processing of Rim101. These results suggest that the interactions between V domains and YP(X)nL motif-containing proteins are conserved from yeast to mammalian cells. Moreover, the specificities of each V domain to their target protein suggest that unidentified elements determine the binding specificity. © 2015, American Society for Microbiology. All Rights Reserved.

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