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Bohra P.,University of Agricultural Sciences, Bangalore | Bohra P.,Central Island Agricultural Research Institute | Waman A.A.,University of Agricultural Sciences, Bangalore | Waman A.A.,Central Island Agricultural Research Institute | And 3 more authors.
Proceedings of the National Academy of Sciences India Section B - Biological Sciences | Year: 2016

The commercial scale banana multiplication through micropropagation is concentrated mainly on the Cavendish group of bananas. Other varieties, though possess a wide array of desirable characters, have been conveniently neglected owing to their inherent problems such as difficult aseptic establishment, low multiplication rate, culture browning, difficult hardening, etc. The mixed diploid bananas including the Ney Poovan are among the choicest of bananas relished in many parts of the world. To promote its cultivation, systematic studies were undertaken and the lines possessing superior attributes have been identified. In order to multiply the clones in large number, a viable multiplication protocol is a must. The present study dealt with optimizing the plant growth regulator requirements of the growing cultures for obtaining superior multiplication and in vitro rooting. Out of the different cytokinins as benzyl aminopurine, 2-isopentenyl adenine, meta topolin (mT), and thidiazuron (TDZ) tested, only TDZ was found to promote shoot multiplication in both types of propagules i.e. single and double bud explants, although shorter plantlets were noticed in the treatment. In vitro root induction parameters were found to be superior when indole butyric acid was used as auxin source. This report could help in improving the multiplication rate of this least attempted group of bananas. As per the present knowledge it is the first report on the use of TDZ and mT in mixed diploid banana. © 2014, The National Academy of Sciences, India. Source


Waman A.A.,University of Agricultural Sciences, Bangalore | Bohra P.,University of Agricultural Sciences, Bangalore | Sathyanarayana B.N.,University of Agricultural Sciences, Bangalore | Gourish R.K.,University of Horticultural science Bagalkot | Ashok T.H.,University of Agricultural Sciences, Bangalore
Proceedings of the National Academy of Sciences India Section B - Biological Sciences | Year: 2014

Panama wilt, a deadly disease of banana in general and Silk group in particular, is creating havoc worldwide. Infected planting material being a major mode of disease spread, production of disease free quality planting material is a must for area expansion. Micropropagation offers a viable solution for overcoming this problem. Though protocols for micropropagation have been standardized for various varieties of banana, their in field trials along with the conventional propagules have seldom been made. This study deals with agronomic, phenological and post harvest attributes of in vitro propagated plantlets of Silk banana var. Nanjanagud Rasabale in comparison to their traditional counterparts. Tissue cultured plantlets were superior to sucker derived plants in terms of vegetative growth, yield and quality parameters. Further micropropagules were precocious by eight weeks and exhibited synchronized flowering. © 2014 The National Academy of Sciences, India. Source


Bohra P.,University of Agricultural Sciences, Bangalore | Waman A.A.,University of Agricultural Sciences, Bangalore | Sathyanarayana B.N.,University of Agricultural Sciences, Bangalore | Umesha K.,University of Horticultural science Bagalkot
Indian Journal of Natural Products and Resources | Year: 2013

Banana, the plant of virtues, is known for its multifaceted uses. Suckers, apart from being used for propagation, are known to have special significance during various religious ceremonies in India. Also, in the absence of commercially viable tissue culture protocols, vegetative propagation through suckers is the only option for conserving and multiplying the native banana varieties. Efforts were thus made to assess the variability amongst two native-export suited varieties and their clones for sucker production. Of the 24 and 37 lines of 'Nanjanagud Rasabale' and 'Elakki Bale' studied, five and nine lines, respectively were found to be promising in terms of their sucker production capacity. Maximum numbers of suckers were produced in the plants during 8 to 10 months of age; thus, farmers may adjust the planting timing so as to get maximum suckers for selling either as planting material or for other uses. This could be a boon for many farmers even in the condition of accidental crop loss due to various biotic or abiotic factors. Further, it may serve as an impetus for export of these varieties. Source


Bohra P.,University of Agricultural Sciences, Bangalore | Bohra P.,Central Island Agricultural Research Institute | Waman A.A.,University of Agricultural Sciences, Bangalore | Waman A.A.,Central Island Agricultural Research Institute | And 4 more authors.
Erwerbs-Obstbau | Year: 2015

Banana, one of the major staple food crops of the world, flowers round the year irrespective of the season. It gives the decisive flexibility to the grower for planning orchard operations as well as exploring the best marketing opportunities for the produce well in advance. Being a heavy feeder of nutrients and water, these inputs are critical to the success of banana cultivation especially at reproductive stage. Also, banana fruits are sensitive to many biotic and abiotic stresses which render them unsuitable for marketing. All these factors could be taken care of if phenological cycle of the crop is known to the grower. In Ney Poovan (Musa AB) banana, 58 plants were studied for parameters like days taken for floral initiation, anthesis, completion of female phase, fruit filling and total crop duration using BBCH scale. Inflorescence appeared after 311 days of planting and duration of reproductive stage varied between 124–147 days with average crop duration of 443 days. An account of the practical implications of this study has been discussed in detail. Further, another study was conducted in order to study the changes in the quality and storage behavior of the fruits harvested at different phases of bunch development (12–18 weeks after anthesis). Studies revealed that fruits harvested after 16 weeks of anthesis were more firm, exhibited maximum shelf life with minimum PLW and had highest TSS content amongst all the groups studied. The early as well as delayed harvesting rendered the fruits inferior in most of the parameters studied. © 2014, Springer-Verlag Berlin Heidelberg. Source


Waman A.A.,University of Agricultural Sciences, Bangalore | Waman A.A.,ICAR Central Island Agricultural Research Institute | Bohra P.,University of Agricultural Sciences, Bangalore | Bohra P.,ICAR Central Island Agricultural Research Institute | And 5 more authors.
Erwerbs-Obstbau | Year: 2015

Factors concerning aseptic culture establishment and hardening were studied in detail in choicest Silk Banana. Effect of size of sucker (small, medium and large), carbon concentration (10, 20 and 30 g/l), season of initiation (wet and dry) and pre-treatments such as segmentation and incision to the explants were studied during initiation. Further, hardening related factors such as substrates used for primary and secondary hardening, nursery nutrition (source and frequency of application) and pre-treatments for ex vitro rooting were also studied. Results revealed that small suckers were most suited for initiations with the least contamination, maximum establishment and higher percentage of greening. Lower concentration of sucrose, though delayed greening, resulted in 100 % establishment of explants. Initiations performed during the drier period were completely free from the fungal contamination and showed less bacterial contamination than those performed during the rainy season. Segmentation of explant into four parts during first subculture supported maximum shoot proliferation by overcoming apical dominance. Coir pith was observed to be the most congenial substrate during primary hardening, whereas coir pith alone or sand: red earth: coir pith (1:1:1) supported superior performance of plantlets during secondary hardening. Single application of mono ammonium phosphate improved growth of plantlets during secondary hardening. Pre-treatment with mono ammonium phosphate gave the best response in terms of rooting and hardening of un-rooted micro-shoots. Thus, the discussed methodology could help the industries to take up commercial scale propagation of Silk Banana. © 2015, Springer-Verlag Berlin Heidelberg. Source

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