Malmasi A.,University of Tehran |
Ardestani B.Z.,University of Tehran |
Mohebali M.,Tehran University of Medical Sciences |
Akhoundi B.,Tehran University of Medical Sciences |
And 8 more authors.
Iranian Journal of Pharmaceutical Research | Year: 2014
Toxicity and drug resistance against pentavalent antimonials, medications of choice in treatment of leishmaniasis for more than 5 decades, have become important subjects globally.This study was a randomized, open labeled trial that was designed to determine efficacy and safety of IMOD as a novel herbal immunomodulator drug for treatment of canine visceral leishmaniasis (CVL).Twenty healthy mongrel dogs were infected with Iranian strain of L. Infantum amastigotes and randomly divided to 5 groups with four animals for each included on: I: negative control (non-infected) II: Glucantime® III: Glucantime® plus IMOD (immune-chemotherapy) IV: IMOD and V: positive control (non-treated). Physical examination, hematological, biochemical, serological, parasitological, pathological and imaging evaluations were performed pre-/postinterventions every month for 3 months.Comparing with control groups (I&V), immune-chemotherapy group (Glucantime® plus IMOD) showed significantly higher efficacy in resolving the clinical signs and hematobiochemistry factors. Based on our results, using IMOD in combination with meglumine antimoniate (Glucantime®) has significantly improved CVL than the latter drug alone. So, it seems this new herbal medicine is useful as adjuvant therapy for canine visceral leishmaniasis. © 2014 by School of Pharmacy.
Pourkazemi M.,International Sturgeon Research Institute |
Nazari S.,University of Gilan |
Bakhshalizadeh S.,University of Gilan
Iranian Journal of Fisheries Sciences | Year: 2010
Preparation of chromosome spreads and karyotype analysis in Blicca bjoerkna transcaucasica were carried out using 0.01% solution of colchicines and Phytohaemagglutinin (PHA) (20 μg g -1 body weight). The gill and kidney tissues were collected and let to stand in a hypotonic solution of 0.075 M KCl and then treated with a fixative (Carnoy's solution) in three steps. The chromosomes spreads were then stained with 5% Giemsa solution for 20 min and examined under a light microscope. Appropriate metaphase plates were photographed in order to prepare karyotype. The size of the chromosomes (short and long arms), relative length of chromosomes and centromere index were calculated. Chromosome spreads from gill tissue cultures which were colchicine treated with PHA, had a well defined size, shape and number of chromosomes for karyotype analysis. Based on the 76 metaphase plates studied, chromosome count in 59 metaphase plates was 2n=49.74±0.25. By arranging homologous chromosomes beside each other the chromosome formula was calculated as 6 pairs of Metacentric, 10 pairs of Sub-Metacentric and 9 pairs of Sub-Telocentric (2n=6M+10+Sm+9St) and the chromosome arm number (NF) was 100. The largest chromosome in this species was a pair of metacentric chromosomes. On the basis of the number and type of chromosomes, the karyotype obtained for this species conformed to the findings of other researchers, but the chromosome formula was different, which could be attributed to the existence of different populations for this species.
Sharifi A.,University of Jiroft |
Gorji M.,University of Tehran |
Asadi H.,University of Gilan |
Pourbabaee A.A.,University of Tehran
Paddy and Water Environment | Year: 2014
The aim of this study was to evaluate the impacts of land leveling on some of soil properties. Land leveling as an effective strategy for paddy land management is one of the most important programs run by the Ministry of Jihad-Agriculture in Guilan province, northern part of Iran. Precise land leveling improves irrigation application, fertilization efficiency, and uniform soil moisture for germination. Also, it increases yields and cultivable lands and reduces delivery losses. Running land-leveling projects, however, leads to such disadvantages as severe soil alteration and consequently disruptions to the soil physical, chemical, and biological properties. Based on statistical analysis, on the one hand land, leveling led to significant increases (P < 0.01) in bulk density, clay percentage, and EC, and on the other hand, it resulted in significant decreases (P < 0.01) in the soil organic matter, silt percentage, NPK, pH, soil respiration, bacterial community, and microbial biomass carbon. Microscopic images of buried slides indicated that there was a reduction in the soil microorganism communities and diversities as well. With regard to the management of paddy fields, despite beneficial impacts of land leveling, the results indicate that due to malperformance of the project, which is a consequence of the use of inappropriate machinery and/or nonremoval of surface soil and its re-diffusion, most of soil-desirable properties are reduced and are not revitalized even after 4 years. © 2013 Springer Japan.
Eslami L.,Golestan University of Medical Sciences |
Isazadehfar K.,Ardabil University of Medical Sciences |
Karami M.,Shahid Beheshti University |
Eslami L.,University of Gilan |
And 2 more authors.
Govaresh | Year: 2015
Background: Despite the prevalence of Non-Alcoholic Fatty Liver Disease (NAFLD) and Non-Alcoholic Steatohepatitis (NASH), there was no treatment has been proven to be effective in these common diseases. Although many studies have shown that lifestyle modifications such as increasing physical activities and exercise could be effective in the treatment of these common diseases, the optimal strategy was still not determined. According to the beneficial effects of antioxidant agents in the treatment of NASH, vitamin E has been used for this purpose by some clinicians. We designed this study for assessing beneficial effects of regular physical activity on the biochemical and imaging responses in patients with NASH and comparing this with vitamin E as an accepted treatment for NASH. Materials and Methods: This study was Randomized and single-blind clinical trials were carried out in Gonbad-e Kavus through which a total of 30 consecutive patients with the ultra sonographic diagnosis of non-alcoholic steatohepatitis (NASH)were enrolled and randomized to one of the three groups: Vitamin E 800 mg/day, regular physical activity, or both. Results: In all treatment groups improvement in liver transaminases level, serum lipids and ultrasonographic grading of fatty liver occurred after three months of treatment. When these decrement was compared between the treatment groups, there was no statistically significant difference in the value of improvement between the three groups (ANOVA: p>0.5). I.e. all three interventions improved the biochemical and ultrasonographic finding of fatty liver in the same way. Both groups with regular exercise had significant mean weight loss in comparison with the vitamin E group (a mean decrease of 3.0 kg in exercise group, 5.8 kg in subjects on regular exercise plus vitamin E and 0.2 kg in vitamin E group, ANOVA: p=0.04). Conclusion: There were no significant differences between exercise and vitamin E alone or in combination regarding the reduction in the level of liver enzymes and sonographic evidences of fatty liver although both resulted in significant improvements in biochemical endpoints. This implies that physical activity could be considered as effective as vitamin E in the improvement of biochemical and ultrasonographic presentations of NASH and the addition of Vitamin E does not offer any benefits. According to the findings of this pilot study a full-powered study with a control group should be designed. © 2015, Iranian Association of Gastroenterology and Hepatology. All rights reserved.
Ghorbani M.-J.,University of Gilan |
Salehi Z.,University of Gilan |
Ejtehadi F.,Shiraz University of Medical Sciences
Journal of Mazandaran University of Medical Sciences | Year: 2014
Background and purpose: Helicobacter pylorus is a well-recognized cause of chronic active gastritis, peptic ulcer disease (PUD) and linked to the development of gastric adenocarcinoma. Heat shock protein (HSP) acts as molecular chaperons in the folding of newly synthesized proteins in cells and assist in the refolding of damaged proteins. The HSP70-2 gene has a pst1 site due to an A to G transition at the 1267 position and different genotypes of the HSP70-2 gene have been shown to be associated with a different level of HSP70 mRNA expression. The aim of this study was to investigate the association between polymorphism of the HSP70-2 gene and susceptibility to helicobacter pylori infection.Materials and methods: The studied population comprised of 100 subjects, attending the Endoscopy Center of Hafez Hospital in Shiraz, Iran. All the subjects underwent upper gastroscopy. RFLP (restriction fragment length polymorphism) analysis was performed for 1267G/A polymorphism of HSP70-2 gene in all the subjects.Results: After gastroscopy, 50 cases with H. pylori infection and 50 cases with normal gastric tissues were considered as the controls. In the normal subjects, the HSP70-2 genotype distribution was 20 AA (40%), 26 AG (52%), and 4 GG (8%). Meanwhile, the HSP70-2 genotype distribution in patients were 5 AA (10%), 43 AG (86%) and 2 GG (4%).Conclusion: The analysis showed that the AG genotype increased the risk of peptic ulcer (OR = 6.61, 95% CI = 2.21-19.76, P = 0.0007). The results of this study suggested that HSP70-2 polymorphism may be involved in susceptibility to helicobacter pylori infection. © 2014 Mazandaran University of Medical Sciences. All rights reserved.
Shabanzadeh P.,University Putra Malaysia |
Senu N.,University Putra Malaysia |
Shameli K.,University Putra Malaysia |
Shameli K.,Iranian Materials and Energy Research Center |
Tabar M.M.,University of Gilan
Journal of Chemistry | Year: 2013
Artificial neural network (ANN) models have the capacity to eliminate the need for expensive experimental investigation in various areas of manufacturing processes, including the casting methods. An understanding of the interrelationships between input variables is essential for interpreting the sensitivity data and optimizing the design parameters. Silver nanoparticles (Ag-NPs) have attracted considerable attention for chemical, physical, and medical applications due to their exceptional properties. The nanocrystal silver was synthesized into an interlamellar space of montmorillonite by using the chemical reduction technique. The method has an advantage of size control which is essential in nanometals synthesis. Silver nanoparticles with nanosize and devoid of aggregation are favorable for several properties. In this investigation, the accuracy of artificial neural network training algorithm was applied in studying the effects of different parameters on the particles, including the AgNO3 concentration, reaction temperature, UV-visible wavelength, and montmorillonite (MMT) d-spacing on the prediction of size of silver nanoparticles. Analysis of the variance showed that the AgNO3 concentration and temperature were the most significant factors affecting the size of silver nanoparticles. Using the best performing artificial neural network, the optimum conditions predicted were a concentration of AgNO 3 of 1.0 (M), MMT d-spacing of 1.27 nm, reaction temperature of 27°C, and wavelength of 397.50 nm. © 2013 Parvaneh Shabanzadeh et al.
Mostashari S.M.,University of Gilan |
Baie S.,University of Gilan
Cellulose Chemistry and Technology | Year: 2010
The effect of lithium bromide as compared to lithium chloride, as non-durable finishes, on the flammability of 100% cotton fabric (woven construction, weighing 151 g/m2) has been investigated in the present article. The laundered, bone-dried, weighed specimens were impregnated with suitable concentrations of aqueous lithium bromide and/or lithium chloride solutions by means of squeeze rolls and then dried at 110 °C for 30 min Afterwards they were cooled in a desiccator, re-weighed with an analytical balance and kept under ordinary conditions before carrying out the vertical flame test. The optimum add-on values conferring flame rctardancy, expressed in g anhydrous lithium bromide and lithium chloride per 100 g fabrics, were found to be about 3.85% and 7.5%, respectively. The thermogravimetric analysis (TGA) of pure cotton and of the fabrics supported by salts at optimum additions was performed and the thermograms were then compared and commented. The results obtained proved the superiority of lithium bromide in imparting flame retardancy as compared to lithium chloride. Overall, the additions comply with Free radical theory in explaining flame-retardancy. The results may provide some beneficial data and assessments to be put into practice for commercial purposes, such as in the dope of insulators, plastics and polymers.
Mostashari S.M.,University of Gilan |
Baie S.,University of Gilan
Journal of Thermal Analysis and Calorimetry | Year: 2010
The combined effect between calcium chloride and red phosphorus (RP) on the flame-retardancy of a cotton fabric (woven construction massing 152 g/m2) has been studied in this work. The laundered bone-dried massed samples were impregnated with suitable concentrations of individual aqueous red phosphorus suspensions and/or calcium chloride solutions and some bunches were impregnated with appropriate admixed solutions of the both chemicals. An acceptable synergistic effect was then experienced by using an admixed bath containing 0.20 F red phosphorus and 0.20 M calcium chloride for impartation of flame-retardancy to a cotton fabric. By using a vertical flame spread test the optimum mass of the mixture needed to donate flame-retardancy was obtained to be about 5.88 g anhydrous additives per 100 g dry fabric. Thermogravimetry (TG/DTG) results concerning untreated and treated cotton fabrics at the optimum addition were obtained and their curves were compared and commented, fortifying the flame spread tests outcomes. It can be deduced that the applied treatment functioned as a catalyst at the combustion's temperature of the polymeric substrate and, thermosensibilized the combustion process. This synergism is in favor of green chemistry as well as the economical and industrial view points. © Akadémiai Kiadó, Budapest, Hungary 2009.
Roohi H.,University of Gilan |
Zahiri E.,Payame Noor University
Computational and Theoretical Chemistry | Year: 2012
Structures and thermodynamic properties of complexes formed from interaction between the methimazole (MMI) as an anti-thyroid drug and M z+ (Ca 2+, Mg 2+, Na + and Li +) were investigated at various levels of theory. We have found two types planar and non-planar MMI-M z+ complexes on the potential energy surface. From the Gibbs free energy difference between the planar and non-planar forms of MMI-M z+ complexes, it is found that the non-planar forms are the predominant ones. In addition, stability of MMI-M z+ complexes increases in the following order: Na +