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Gainesville, FL, United States

The University of Florida is an American public land-grant, sea-grant, and space-grant research university located on a 2,000-acre campus in North Central Florida. Howard and Matthew Greene recognized Florida as a Public Ivy in 2001, a publicly funded university considered as providing a quality of education comparable to those of the Ivies. In 2014, U.S. News & World Report ranked Florida as the fourteenth best public university in the United States. It is a senior member of the State University System of Florida and traces its historical origins to 1853, and has operated continuously on its present Gainesville campus since September 1906.The University of Florida is an elected member of the Association of American Universities , the association of preeminent North American research universities. The University is classified as a Research University with Very High Research by the Carnegie Foundation for the Advancement of Teaching. The university is accredited by the Southern Association of Colleges and Schools . Florida Governor Rick Scott and the state Legislature designated the University of Florida as one of two "preeminent" state universities in the spring of 2013. It is the third largest Florida university by student population, and is the eighth largest single-campus university in the United States with 49,913 students enrolled for the fall 2012 semester. The University of Florida is home to sixteen academic colleges and more than 150 research centers and institutes. It offers multiple graduate professional programs—including business administration, engineering, law, dentistry, medicine, and veterinary medicine—on one contiguous campus, and administers 123 master's degree programs and seventy-six doctoral degree programs in eighty-seven schools and departments.The University of Florida's intercollegiate sports teams, commonly known by their "Florida Gators" nickname, compete in National Collegiate Athletic Association Division I and the Southeastern Conference . In their 108-year history, the university's varsity sports teams have won thirty-three national team championships, twenty-eight of which are NCAA titles, and Gator athletes have won 267 individual national championships. Wikipedia.

Sefah K.,University of Florida
Nature protocols | Year: 2010

In the past two decades, high-affinity nucleic acid aptamers have been developed for a wide variety of pure molecules and complex systems such as live cells. Conceptually, aptamers are developed by an evolutionary process, whereby, as selection progresses, sequences with a certain conformation capable of binding to the target of interest emerge and dominate the pool. This protocol, cell-SELEX (systematic evolution of ligands by exponential enrichment), is a method that can generate DNA aptamers that can bind specifically to a cell type of interest. Commonly, a cancer cell line is used as the target to generate aptamers that can differentiate that cell type from other cancers or normal cells. A single-stranded DNA (ssDNA) library pool is incubated with the target cells. Nonbinding sequences are washed off and bound sequences are recovered from the cells by heating cell-DNA complexes at 95 degrees C, followed by centrifugation. The recovered pool is incubated with the control cell line to filter out the sequences that bind to common molecules on both the target and the control, leading to the enrichment of specific binders to the target. Binding sequences are amplified by PCR using fluorescein isothiocyanate-labeled sense and biotin-labeled antisense primers. This is followed by removal of antisense strands to generate an ssDNA pool for subsequent rounds of selection. The enrichment of the selected pools is monitored by flow cytometry binding assays, with selected pools having increased fluorescence compared with the unselected DNA library. The procedure, from design of oligonucleotides to enrichment of the selected pools, takes approximately 3 months. Source

Will C.M.,University of Florida
Living Reviews in Relativity | Year: 2014

The status of experimental tests of general relativity and of theoretical frameworks for analyzing them is reviewed and updated. Einstein's equivalence principle (EEP) is well supported by experiments such as the Eötvös experiment, tests of local Lorentz invariance and clock experiments. Ongoing tests of EEP and of the inverse square law are searching for new interactions arising from unification or quantum gravity. Tests of general relativity at the post-Newtonian level have reached high precision, including the light deflection, the Shapiro time delay, the perihelion advance of Mercury, the Nordtvedt effect in lunar motion, and frame-dragging. Gravitational wave damping has been detected in an amount that agrees with general relativity to better than half a percent using the Hulse-Taylor binary pulsar, and a growing family of other binary pulsar systems is yielding new tests, especially of strong-field effects. Current and future tests of relativity will center on strong gravity and gravitational waves. Source

As obesity reaches epidemic proportions, nonalcoholic fatty liver disease (NAFLD) is becoming a frequent cause of patient referral to gastroenterologists. There is a close link between dysfunctional adipose tissue in NAFLD and common conditions such as metabolic syndrome, type 2 diabetes mellitus, and cardiovascular disease. This review focuses on the pathophysiology of interactions between adipose tissue and target organs in obesity and the resulting clinical implications for the management of nonalcoholic steatohepatitis. The release of fatty acids from dysfunctional and insulin-resistant adipocytes results in lipotoxicity, caused by the accumulation of triglyceride-derived toxic metabolites in ectopic tissues (liver, muscle, pancreatic beta cells) and subsequent activation of inflammatory pathways, cellular dysfunction, and lipoapoptosis. The cross talk between dysfunctional adipocytes and the liver involves multiple cell populations, including macrophages and other immune cells, that in concert promote the development of lipotoxic liver disease, a term that more accurately describes the pathophysiology of nonalcoholic steatohepatitis. At the clinical level, adipose tissue insulin resistance contributes to type 2 diabetes mellitus and cardiovascular disease. Treatments that rescue the liver from lipotoxicity by restoring adipose tissue insulin sensitivity (eg, significant weight loss, exercise, thiazolidinediones) or preventing activation of inflammatory pathways and oxidative stress (ie, vitamin E, thiazolidinediones) hold promise in the treatment of NAFLD, although their long-term safety and efficacy remain to be established. Better understanding of pathways that link dysregulated adipose tissue, metabolic dysfunction, and liver lipotoxicity will result in improvements in the clinical management of these challenging patients. © 2012 AGA Institute. Source

Okun M.S.,University of Florida
New England Journal of Medicine | Year: 2012

A 72-year-old right-handed man with a 12-year history of Parkinson's disease presents with a diminished response to medication and right-sided dyskinesia (involuntary movements). During the past several years, he has been taking multiple drugs for Parkinson's disease, including a monoamine oxidase inhibitor, amantadine, a dopamine agonist, and carbidopa-levodopa. He reports that with his current regimen, which includes 1.5 tablets of 25/100 carbidopa-levodopa taken every 2 hours, he has marked reductions in tremor, rigidity, and bradykinesia and substantial improvement in his walking. Despite multiple interval and dose adjustments, however, he also reports 6 hours per day of "off" time, when his symptoms are unresponsive to his current medication regimen. In addition, he has severe, disabling right-sided dyskinesia 4 hours per day. Symptoms affecting his left side are mild and not bothersome. His cognition is excellent, his neurologic examination is otherwise normal, and he has no other coexisting medical conditions. His neurologist refers him to a neurosurgeon for consideration of deep-brain stimulation. Copyright © 2012 Massachusetts Medical Society. Source

Maupin-Furlow J.A.,University of Florida
Annual Review of Microbiology | Year: 2014

Prokaryotes form ubiquitin (Ub)-like isopeptide bonds on the lysine residues of proteins by at least two distinct pathways that are reversible and regulated. In mycobacteria, the C-terminal Gln of Pup (prokaryotic ubiquitin-like protein) is deamidated and isopeptide linked to proteins by a mechanism distinct from ubiquitylation in enzymology yet analogous to ubiquitylation in targeting proteins for destruction by proteasomes. Ub-fold proteins of archaea (SAMPs, small archaeal modifier proteins) and Thermus (TtuB, tRNA-two-thiouridine B) that differ from Ub in amino acid sequence, yet share a common β-grasp fold, also form isopeptide bonds by a mechanism that appears streamlined compared with ubiquitylation. SAMPs and TtuB are found to be members of a small group of Ub-fold proteins that function not only in protein modification but also in sulfur-transfer pathways associated with tRNA thiolation and molybdopterin biosynthesis. These multifunctional Ub-fold proteins are thought to be some of the most ancient of Ub-like protein modifiers. Copyright © 2014 by Annual Reviews. All rights reserved. Source

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