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Brno, Czech Republic

Tichy A.,University of Defence at Brno
Acta medica (Hradec Králové) / Universitas Carolina, Facultas Medica Hradec Králové | Year: 2010

Ataxia-telangiectasia mutated kinase (ATM) is a DNA damage-inducible protein kinase, which phosphorylates plethora of substrates participating in DNA damage response. ATM significance for the cell faith is undeniable, since it regulates DNA repair, cell-cycle progress, and apoptosis. Here we describe its main signalling targets and discuss its importance in DNA repair as well as novel findings linked to this key regulatory enzyme in the terms of ionizing radiation-induced DNA damage.

Valis D.,University of Defence at Brno | Pietrucha-Urbanik K.,Rzeszow University of Technology
Eksploatacja i Niezawodnosc | Year: 2014

Assessing the vulnerability of critical infrastructure objects is of major concern when dealing with the process of dependability and risk management. Special attention is paid to the objects of higher interest, such as nuclear power plants. In spite of the protection of these objects, there is still a certain level of a potential threat. The aim of the paper is to describe a possible way of attacking on the object in order to get into a particular part of it. Several characteristics of an adversarýs attempt were obtained. For this reason as well as for modelling adversarýs behaviour diffusion processes have been used.

Khateb F.,Brno University of Technology | Biolek D.,University of Defence at Brno
Circuits, Systems, and Signal Processing | Year: 2011

Low-voltage (LV) low-power (LP) integrated circuit design is becoming a leading trend in VLSI technology, particularly in special portable applications. In this paper the principle of a bulk-driven MOS transistor is employed in the design of a novel LV LP current differencing transconductance amplifier (CDTA). Designs in the 0.25 μm CMOS technology have been verified via PSpice simulation. The supply voltages are only ±0.6 V. © 2011 Springer Science+Business Media, LLC.

Zaplatilek K.,University of Defence at Brno
Proceedings of the European Computing Conference, ECC '11 | Year: 2011

The article deals with various ways of memristor modeling and simulation in the MATLAB®&Simulink® environment. Recently used and published mathematical memristor model serves as a base, regarding all known features of its behavior. Three different approaches in the MATLAB&Simulink system are used for the differential and other equations formulation. The first one employs the standard system core offer for the Ordinary Differential Equations solutions (ODE) in the form of an m-file. The second approach is the model construction in Simulink environment. The third approach employs so-called physical modeling using the built-in Simscape™ system. The output data are the basic memristor characteristics and appropriate time courses. The features of all models are discussed, especially regarding the computer simulation. Possible problems that may occur during modeling are pointed.

Tichy A.,University of Defence at Brno
Acta medica (Hradec Králové) / Universitas Carolina, Facultas Medica Hradec Králové | Year: 2012

In this paper we describe the influence of NU7026, a specific inhibitor of DNA-dependent protein kinase, phosphoinositide 3-kinase, and ATM-kinase on molecular and cellular mechanisms triggered by ionising irradiation in human T-lymphocyte leukaemic MOLT-4 cells. We studied the effect of this inhibitor (10 1microM) combined with gamma-radiation (1 Gy) leading to DNA damage response and induction of apoptosis. We used methods for apoptosis assessment (cell viability count and flow-cytometric analysis) and cell cycle analysis (DNA content measurement) and we detected expression and post-translational modifications (Western blotting) of proteins involved in DNA repair signalling pathways. Pre-treatment with NU7026 resulted into decreased activation of checkpoint kinase-2 (Thr68), p53 (Ser15 and Ser392), and histone H2A.X (Ser139) 2 hours after irradiation. Subsequently, combination of radiation and inhibitor led to decreased amount of cells in G2-phase arrest and into increased apoptosis after 72 hours. Our results indicate that in leukaemic cells the pre-incubation with inhibitor NU7026 followed by low doses of ionising radiation results in radio-sensitising of MOLT-4 cells via diminished DNA repair and delayed but pronounced apoptosis. This novel approach might offer new strategies in combined treatment of leukaemia diseases.

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