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Saltillo, Mexico

Rodriguez-Jasso R.M.,IBB Institute for Biotechnology And Bioengineering | Mussatto S.I.,IBB Institute for Biotechnology And Bioengineering | Pastrana L.,University of Vigo | Aguilar C.N.,University of Coahuila | Teixeira J.A.,IBB Institute for Biotechnology And Bioengineering
Applied Biochemistry and Biotechnology | Year: 2010

Ten different fungal strains from the genus Aspergillus, Penicillium, and Mucor were screened for fucoidan hydrolyzing ability aiming to find microorganisms able to produce sulfated fucan-degrading enzymes. Screening was carried out by measuring the strains kinetic and morphometric behavior over plate assays using Laminaria japonica fucoidan as only carbon source, testing three nitrogen sources (urea, peptone, and sodium nitrate). The selected fungal strains were subsequently used in submerged fermentations, which were performed for (1) selection of the strains able to growth over fucoidan medium and (2) media selection, testing the synergy of fucoidan with other sugars for inducing high enzyme titles. Radial expansion and hyphae parameters were observed for Aspergillus niger PSH, Mucor sp. 3P, and Penicillium purpurogenum GH2 grown only over fucoidan-urea medium. A. niger PSH showed the maximum enzymatic activity values, which were significantly different (p<0.05) from those achieved by the other selected fungi. Sucrose addition to fucoidan media proportioned the highest fucoidanase activity values for this fungal strain. This research allowed establishing optimal conditions for metabolites synthesis by fungal stains able to act toward fucoidan ramified matrix. © 2010 Springer Science+Business Media, LLC. Source

Recio-Vega R.,University of Coahuila | Recio-Vega R.,Environmental Health Unit | Valdez-Abrego C.,Met Mex Penoles Company | Adame-Lopez B.,Environmental Health Unit | Gurrola-Mendez A.,Met Mex Penoles Company
International Journal of Hygiene and Environmental Health | Year: 2012

Introduction: Because children exposed to lead have a very high health risk, surveillance and prevention programs are very important to avoid short- and long-term health effects. Objectives: To describe the trend for the blood lead levels over a 12-year period in environmentally exposed children and to document the actions implemented to reduce the blood lead levels. Materials and methods: We performed a retrospective cohort study of children aged 0-15 years who were enrolled in the Coahuila Health Secretary's Childhood Blood Lead Level Surveillance program. This database includes children from the city of Torreon, Coahuila, Mexico, where the biggest smelter in Latin America is located. Results: A total of 151,322 observations were analyzed in the study. The percentage of samples with elevated blood lead levels decreased from 84.9% to 10.4% during 1998-2010, and the decrease was greater in girls than in boys. Conclusion: According to the results of our study, the majority of strategies and activities to decrease blood lead levels in an environmentally exposed population should be focused on children aged 0-5 years, on the home environment, on preventing fugitive emissions from smelters and other sources and on the proper disposal and confinement of industrial residues. © 2011 Elsevier GmbH. Source

Wiegratz I.,MVZ Kinderwunschzentrum Wiesbaden GmbH | Wiegratz I.,Goethe University Frankfurt | Bassol S.,University of Coahuila | Weisberg E.,University of Sydney | And 2 more authors.
Reproductive Sciences | Year: 2014

This Phase III, uncontrolled, open-label, multicenter study was conducted to investigate the contraceptive efficacy, bleeding pattern, and cycle control of a novel once-a-week contraceptive patch, delivering low-dose ethinyl estradiol (EE) and gestodene (GSD) at the same systemic exposure seen after oral administration of a combined oral contraceptive containing 0.02 mg EE/0.06 mg GSD. Participants were women aged 18 to 35 years, all of whom received the EE/GSD patch for 13 cycles each of 21 treatment days (one patch per week for 3 weeks) followed by a 7-day, patch-free interval. The primary efficacy variable was the occurrence of unintended pregnancies during the study period as assessed by life table analysis and the Pearl Index. Secondary efficacy variables were days with bleeding during four 90-day reference periods and during 1 treatment year, bleeding pattern, and cycle control. The Kaplan-Meier probability of contraceptive protection after 364 treatment days was 98.8% and the adjusted Pearl Index was 0.81. The percentage of participants with intracyclic bleeding/spotting decreased over time, from 11.4% to 6.8% in cycles 1 and 12, respectively. Almost all participants (range: 90.8%-97.6%) experienced withdrawal bleeding across the study period. Compliance was very high (mean: 97.9%; median: 100%). The most frequent adverse events were headache (9.5%) and application site reaction (8.5%); no clinically significant safety concerns were observed. Results suggest the EE/GSD patch is highly effective in preventing pregnancy. Menstrual bleeding pattern was favorable and within the ranges expected of a healthy female population. The patch was well tolerated and treatment compliance was high. © The Author(s) 2014. Source

Loredo-Trevino A.,University of Coahuila | Gutierrez-Sanchez G.,University of Georgia | Rodriguez-Herrera R.,University of Coahuila | Aguilar C.N.,University of Coahuila
Journal of Polymers and the Environment | Year: 2012

Plastics are present in a lot of aspects of everyday life. They are very versatile and resistant to microbial attack. Polyurethanes are used in several industries and are divided in polyester and polyether polyurethanes and there are different types among them. Despite their microbial resistance, they are susceptible to the attack of fungi and bacteria but the mechanism to elucidate its biodegradation are unknown. There are reports from bacteria and fungi that are capable of degrading polyurethane but the studies about the enzymes that attack the plastic are focused on bacterial enzymes only. The enzymes reported are of type esterase and protease mainly since these enzymes are very unspecific and can recognize some regions in the polyurethane molecule and hydrolyze it. Fungal enzymes have been studied prior the 1990s decade but recently, some authors report the use of filamentous fungi to degrade polyurethane and also report some characteristics of the enzymes involved in it. This review approaches polyurethane biodegradation by focusing on the enzymes reported to date. © 2011 Springer Science+Business Media, LLC. Source

Recio-Vega R.,University of Coahuila | Gonzalez-Cortes T.,University of Coahuila | Olivas-Calderon E.,University of Coahuila | Lantz R.C.,University of Arizona | And 2 more authors.
Journal of Applied Toxicology | Year: 2015

The lung is a target organ for adverse health outcomes following exposure to As. Several studies have reported a high prevalence of respiratory symptoms and diseases in subjects highly exposed to As through drinking water; however, most studies to date has been performed in exposed adults, with little information on respiratory effects in children. The objective of the study was to evaluate the association between urinary levels of As and its metabolites with lung function in children exposed in utero and in early childhood to high As levels through drinking water. A total of 358 healthy children were included in our study. Individual exposure was assessed based on urinary concentration of inorganic As. Lung function was assessed by spirometry. Participants were exposed since pregnancy until early childhood to an average water As concentration of 152.13μgl-1. The mean urinary As level registered in the studied subjects was 141.2μgl-1 and only 16.7% had a urinary concentration below the national concern level. Forced vital capacity was significantly decreased in the studied population and it was negatively associated with the percentage of inorganic As. More than 57% of the subjects had a restrictive spirometric pattern. The urinary As level was higher in those children with restrictive lung patterns when compared with the levels registered in subjects with normal spirometric patterns. Exposure to As through drinking water during in utero and early life was associated with a decrease in forced vital capacity and with a restrictive spirometric pattern in the children evaluated. © 2014 John Wiley & Sons, Ltd. Source

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