University of Bouake

University of Technology of Compiègne, France

University of Bouake

University of Technology of Compiègne, France
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Ayouba A.,Montpellier University | Akoua-Koffi C.,University of Bouake | Calvignac-Spencer S.,Project Group Epidemiology of Highly Pathogenic Microorganisms RKI | Esteban A.,Montpellier University | And 7 more authors.
AIDS | Year: 2013

HIV types 1 and 2 (HIV-1 and HIV-2) are the result of multiple cross-species transmissions of their simian counterparts (SIVs) to humans. We studied whether new SIVs lineages have been transmitted to humans in rural Côte d'Ivoire and identified a novel HIV-2 variant (HIV-2-07IC-TNP03) not related to any of the previously defined HIV-2 groups. This finding shows that sooty mangabey viruses continue to be transmitted to humans, causing new zoonotic outbreaks. © 2013 Wolters Kluwer Health | Lippincott Williams & Wilkins.


Ahoua Alou L.P.,University Of Cocody | Koffi A.A.,Institute Pierre Richet IPR | Adja M.A.,University Of Cocody | Tia E.,University Of Bouake | And 3 more authors.
Malaria Journal | Year: 2010

Background. The spread of pyrethroid resistance in Anopheles gambiae s.s. is a critical issue for malaria vector control based on the use of insecticide-treated nets. Carbamates and organophosphates insecticides are regarded as alternatives or supplements to pyrethroids used in nets treatment. It is, therefore, essential to investigate on the susceptibility of pyrethroid resistant populations of An. gambiae s.s. to these alternative products. Methods. In September 2004, a cross sectional survey was conducted in six localities in Côte d'Ivoire: Toumbokro, Yamoussoukro, Toumodi in the Southern Guinea savannah, Tiassalé in semi-deciduous forest, then Nieky and Abidjan in evergreen forest area. An. gambiae populations from these localities were previously reported to be highly resistant to pyrethroids insecticides. Anopheline larvae were collected from the field and reared to adults. Resistance/susceptibility to carbamates (0.4% carbosulfan, 0.1% propoxur) and organophosphates (0.4% chlorpyrifos-methyl, 1% fenitrothion) was assessed using WHO bioassay test kits for adult mosquitoes. Then, PCR assays were run to determine the molecular forms (M) and (S), as well as phenotypes for insensitive acetylcholinesterase (AChE1) due to G119S mutation. Results. Bioassays showed carbamates (carbosulfan and propoxur) resistance in all tested populations of An. gambiae s.s. In addition, two out of the six tested populations (Toumodi and Tiassalé) were also resistant to organophosphates (mortality rates ranged from 29.5% to 93.3%). The M-form was predominant in tested samples (91.8%). M and S molecular forms were sympatric at two localities but no M/S hybrids were detected. The highest proportion of S-form (7.9% of An. gambiae identified) was in sample from Toumbokro, in the southern Guinea savannah. The G119S mutation was found in both M and S molecular forms with frequency from 30.9 to 35.2%. Conclusion. This study revealed a wide distribution of insensitive acetylcholinesterase due to the G119S mutation in both M and S molecular forms of the populations of An. gambiae s.s. tested. The low cross-resistance between carbamates and organophosphates highly suggests involvement of other resistance mechanisms such as metabolic detoxification or F290V mutation. © 2010 Alou et al; licensee BioMed Central Ltd.


Ouattara A.F.,Swiss Tropical and Public Health Institute | Ouattara A.F.,University of Basel | Dagnogo M.,Nangui Abrogoua University | Constant E.A.,Nangui Abrogoua University | And 9 more authors.
Malaria Journal | Year: 2014

Background: The use of long-lasting insecticidal nets (LLINs) is an effective malaria control strategy. However, there are challenges to achieve high coverage, such as distribution sustainability, and coverage keep-up. This study assessed the effect of LLINs coverage and contextual factors on entomological indicators of malaria in rural Côte d'Ivoire. Methods. The study was carried out between July 2009 and May 2012 in three villages (Bozi, N'Dakonankro and Yoho) of central Côte d'Ivoire. In Bozi and Yoho, LLINs were distributed free of charge by the national malaria control programme in 2008. In Bozi, an additional distribution was carried out in May 2011. No specific interventions were done in N'Dakonankro. Entomological surveys were conducted in July 2009 and July 2010 (baseline), and in August and November 2011 and in February 2012. Frequency of circumsporozoite protein was determined using an enzyme-linked immunosorbent assay. Regression models were employed to assess the impact of LLINs and changing patterns of irrigated rice farming on entomological parameters, and to determine associations with LLINs coverage and other contextual factors. Results: In Bozi, high proportion of LLIN usage was observed (95-100%). After six months, 95% of LLINs were washed at least once and 79% were washed up to three times within one year. Anopheles gambiae was the predominant malaria vector (66.6% of all mosquitoes caught). From 2009 to 2012, in N'Dakonankro, the mean annual entomological inoculation rate (EIR) increased significantly from 116.8 infectious bites/human/year (ib/h/y) to 408.8 ib/h/y, while in the intervention villages, the EIR decreased significantly from 514.6 ib/h/y to 62.0 ib/h/y (Bozi) and from 83.9 ib/h/y to 25.5 ib/h/y (Yoho). The risk of an infectious bite over the three-year period was significantly lower in the intervention villages compared to the control village (p <0.001). Conclusion: High coverage and sensitization of households to use LLINs through regular visits (particularly in Bozi) and abandoning irrigated rice farming (in Yoho) resulted in highly significant reductions of EIR. The national malaria control programme should consider household sensitization and education campaigns and other contextual factors to maximize the benefit of LLINs. © 2014 Ouattara et al.; licensee BioMed Central Ltd.


Ballet J.,University of Versailles | Koffi J.-M.,UMI Resiliences | Koffi J.-M.,University of Bouake | Pelenc J.,UMI Resiliences
Ecological Economics | Year: 2013

In recent years, several studies have attempted to combine the capabilities approach with sustainable development. However, critics have pointed out that although the capability approach takes the environment into account, it has its shortcomings for not being a complete ethical theory. Our article attempts to go beyond these criticisms, and show that the capabilities approach provides a good analytical framework for an environmental justice approach. © 2012 Elsevier B.V.


Momou K.J.,Nangui Abrogoua University | Akoua-Koffi C.,University Of Bouake | Dosso M.,Institute Pasteur Of Cote Divoire
Food and Environmental Virology | Year: 2014

The objective of this study was to compare sensitivities of enterovirus isolation from wastewater in different cell lines as well as to compare the sensitivity and specificity of isolation in cell culture with direct detection by reverse transcription polymerase chain reaction (RT-PCR). Sixty-eight samples of wastewaters were collected between September 2008 and January 2009 in Yopougon, Abidjan. Enteroviruses were concentrated according to World Health Organization recommendations. Viruses were inoculated into various cell lines while direct RT-PCR was performed on water concentrates. The buffalo green monkey kidney cell line was the most sensitive with 58.8 % of viral isolation. This was followed by the rhabdomyosarcoma cell line with sensitivity of 51.6 %, with human epidermoid carcinoma cell line showing sensitivity of 50 % and fibroblastic cells derived from transgenic mice LTK-1 (L20B) cell showing 23.50 % sensitivity. However, a lower specificity of 2.9 % was observed with the L20B cell line. 44.1 % of the samples were positive by direct RT-PCR detection while 51.47 % samples were positive by using RT-PCR on infected cell cultures. No difference in percentage positivity was observed using RT-PCR on infected tissue culture isolates or using RT-PCR directly on wastewater samples. © 2013 Springer Science+Business Media New York.


PubMed | Institute Pasteur Of Cote Divoire and University Of Bouake
Type: Evaluation Studies | Journal: Food and environmental virology | Year: 2016

The objective of this study was to compare sensitivities of enterovirus isolation from wastewater in different cell lines as well as to compare the sensitivity and specificity of isolation in cell culture with direct detection by reverse transcription polymerase chain reaction (RT-PCR). Sixty-eight samples of wastewaters were collected between September 2008 and January 2009 in Yopougon, Abidjan. Enteroviruses were concentrated according to World Health Organization recommendations. Viruses were inoculated into various cell lines while direct RT-PCR was performed on water concentrates. The buffalo green monkey kidney cell line was the most sensitive with 58.8% of viral isolation. This was followed by the rhabdomyosarcoma cell line with sensitivity of 51.6%, with human epidermoid carcinoma cell line showing sensitivity of 50% and fibroblastic cells derived from transgenic mice LTK-1 (L20B) cell showing 23.50% sensitivity. However, a lower specificity of 2.9% was observed with the L20B cell line. 44.1% of the samples were positive by direct RT-PCR detection while 51.47% samples were positive by using RT-PCR on infected cell cultures. No difference in percentage positivity was observed using RT-PCR on infected tissue culture isolates or using RT-PCR directly on wastewater samples.


PubMed | Kwame Nkrumah University Of Science And Technology, University of Washington, University of Bouake and Columbia University
Type: Journal Article | Journal: The British journal of surgery | Year: 2016

This study aimed to describe national peripheral vascular disease (PVD) risk and health burden, and vascular care capacity in Ghana. The gap between PVD burden and vascular care capacity in low- and middle-income countries was defined, and capacity improvement priorities were identified.Data to estimate PVD risk factor burden were obtained from the World Health Organization Study on Global Ageing and Adult Health (SAGE), Ghana, and the Institute of Health Metrics and Evaluation Global Burden of Disease (IHME GBD) database. In addition, a novel nationwide assessment of vascular care capacity was performed, with 20 vascular care items assessed at 40 hospitals in Ghana. Factors contributing to specific item deficiency were described.From the SAGE database, there were 4305 respondents aged at least 50 years with data to estimate PVD risk. Of these, 574 per cent were at moderate to risk high of PVD with at least three risk factors; extrapolating nationally, the estimate was 1654557 people. Based on IHME GBD data, the estimated disability-adjusted life-years incurred from PVD increased fivefold from 1990 to 2010 (from 63 to 317 per 100000 persons respectively). Vascular care capacity assessment demonstrated marked deficiencies in items for diagnosis, and in perioperative and vascular surgical care. Deficiencies were most often due to absence of equipment, lack of training and technology breakage.Risk factor reduction and management as well as optimization of current resources are paramount to avoid the large burden of PVD falling on healthcare systems in low- and middle-income countries. These countries are not well equipped to handle vascular surgical care, and rapid development of such capacity would be difficult and expensive.


Ballet J.,University of Bordeaux 1 | Bazin D.,University of Nice Sophia Antipolis | Koffi K.J.M.,University of Bouake | Komena K.B.,University of Bouake
Society and Natural Resources | Year: 2015

Cultural heterogeneity is considered to be a plausible explanatory factor in the management of natural resources. We show that the capability approach can nuance this argument. By contextualizing the role of cultural heterogeneity, arguing that inequalities of opportunity often take precedence over cultural differences, we show how the capability approach modifies the role of this factor. We then underline that cultural heterogenity is often a mask for differences in opportunities based on three dimensions of power: strategic power, institutional power, and domination power. © 2015, Copyright © Taylor & Francis Group, LLC.


This article examines the effects of the post-2002 sociopolitical crisis in Abidjan, Côte d'Ivoire, on urban and peri-urban agriculture. Based on the case study of Abidjan, it argues for a conceptualization of sustainability that includes social as well as environmental dimensions and focuses on coping strategies of producers and merchants. In Abidjan, these strategies included internal migration within the city and its periphery, the use of organic fertilizers, and changes in market structure. The study illustrates how such strategies allowed producers to continue to supply produce to the market, despite the difficulties of war.


Sociocultural etiology to guide community control of malaria in Côte d'Ivoire In the rural communities of Côte d'Ivoire, malaria control is guided by the sociocultural etiology or perception of this disease, as we can see by studies conducted in the Baoulé and Gouro communities. These studies show that these communities establish a link between the sun, excessive oil consumption, agricultural activities, disease, and lack of hygiene. Mosquitoes, however, are not identified as the cause of malaria but rather as an insect noxious by their noise and painful bites. These populations use both traditional and modern means to move mosquitoes away from their homes. Analysis of this social reality shows that the battle against malaria in rural areas is not simply a problem of health education. It is thus important in developing malaria control programmes to adopt a strategy that links community participation and health education.

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