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Farmsen-Berne, Germany

Broggini T.,Charite - Medical University of Berlin | Czabanka M.,Charite - Medical University of Berlin | Piffko A.,Charite - Medical University of Berlin | Harms C.,Charite - Medical University of Berlin | And 6 more authors.
European Spine Journal | Year: 2015

Introduction: Clinical treatment of spinal metastasis is gaining in complexity while the underlying biology remains unknown. Insufficient biological understanding is due to a lack of suitable experimental animal models. Intercellular adhesion molecule-1 (ICAM1) has been implicated in metastasis formation. Its role in spinal metastasis remains unclear. It was the aim to generate a reliable spinal metastasis model in mice and to investigate metastasis formation under ICAM1 depletion. Material and methods: B16 melanoma cells were infected with a lentivirus containing firefly luciferase (B16-luc). Stable cell clones (B16-luc) were injected retrogradely into the distal aortic arch. Spinal metastasis formation was monitored using in vivo bioluminescence imaging/MRI. Neurological deficits were monitored daily. In vivo selected, metastasized tumor cells were isolated (mB16-luc) and reinjected intraarterially. mB16-luc cells were injected intraarterially in ICAM1 KO mice. Metastasis distribution was analyzed using organ-specific fluorescence analysis. Results: Intraarterial injection of B16-luc and metastatic mB16-luc reliably induced spinal metastasis formation with neurological deficits (B16-luc:26.5, mB16-luc:21 days, p < 0.05). In vivo selection increased the metastatic aggressiveness and led to a bone specific homing phenotype. Thus, mB16-luc cells demonstrated higher number (B16-luc: 1.2 ± 0.447, mB16-luc:3.2 ± 1.643) and increased total metastasis volume (B16-luc:2.87 ± 2.453 mm3, mB16-luc:11.19 ± 3.898 mm3, p < 0.05) in the spine. ICAM1 depletion leads to a significantly reduced number of spinal metastasis (mB16-luc:1.2 ± 0.84) with improved neurological outcome (29 days). General metastatic burden was significantly reduced under ICAM1 depletion (control: 3.47 × 107 ± 1.66 × 107; ICAM-1−/−: 5.20 × 104 ± 4.44 × 104, p < 0.05 vs. control) Conclusion: Applying a reliable animal model for spinal metastasis, ICAM1 depletion reduces spinal metastasis formation due to an organ-unspecific reduction of metastasis development. © 2015, Springer-Verlag Berlin Heidelberg. Source


Sherif C.,Hospital Rudolfstiftung | Sherif C.,Medical University of Vienna | Fandino J.,University of Berne | Erhardt S.,University of Berne | And 4 more authors.
Journal of Visualized Experiments | Year: 2011

For ruptured human cerebral aneurysms endovascular embolization has become an equivalent alternative to aneurysm clipping. 1 However, large clinical trials have shown disappointing long-term results with unacceptable high rates of aneurysm recanalization and delayed aneurysm rupture. 2 To overcome these problems, animal experimental studies are crucial for the development of better endovascular devices. 3-5 Several animal models in rats, rabbits, canines and swine are available. 6-8 Comparisons of the different animal models showed the superiority of the rabbit model with regard to hemodynamics and comparability of the coagulation system and cost-effectiveness. 9-11 The venous pouch arterial bifurcation model in rabbits is formed by a venous pouch sutured into an artificially created true bifurcation of both common carotid arteries (CCA). The main advantage of this model are true bifurcational hemodynamics. 12 The major drawbacks are the sofar high microsurgical technical demands and high morbidity and mortality rates of up to 50%. 13 These limitations have resulted in less frequent use of this aneurysm model in the recent years. These shortcomings could be overcome with improved surgical procedures and modified peri- and postoperative analgetic management and anticoagulation. 14-16 Our techniques reported in this paper demonstrate this optimized technique for microsurgical creation of arterial bifurcation aneurysms. © JoVE 2006-2011 All Rights Reserved. Source


Boss R.,University of Berne | Naskova J.,Agroscope Liebefeld Posieux Research Station | Steiner A.,University of Berne | Graber H.U.,University of Berne
Journal of Dairy Science | Year: 2011

A novel real-time quantitative PCR assay for detecting the pathogenic and contagious Staphylococcus aureus genotype B (GTB) in bulk tank milk was developed and evaluated. The detection of this pathogen in bulk tank milk would greatly facilitate its control, as it is responsible for great economic loss in Swiss dairy herds. The assay is based on the simultaneous detection of 3 GTB-typical target sequences, including 2 enterotoxin genes and a polymorphism within the leucotoxin E gene. A variety of mastitis-associated bacteria was used to validate the assays, resulting in an analytical specificity of 100% and high repeatability. The analytical sensitivity in milk was 40 cfu/mL. An exponential association between simulated cow prevalence and quantitative PCR result was observed. An initial field study revealed 1 GTB-positive herd among the 33 studied herds. This novel assay for bulk tank milk analysis is suitable for routine purposes and is expected to be an effective tool for minimizing Staph. aureus GTB in Swiss dairy herds. © 2011 American Dairy Science Association. Source


Andres R.H.,Stanford University | Andres R.H.,University of Berne | Pendharkar A.V.,Stanford University | Kuhlen D.,University of Berne | And 2 more authors.
Journal of Neurosurgery | Year: 2010

Patients requiring CSF shunts frequently have comorbidities that can influence water and electrolyte balances. The authors report on a case involving a ventriculoperitoneal shunt in a patient who underwent intravenous hyperhydration and withdrawal of vasopressin substitution prior to scheduled high-dose chemotherapy regimen for a metastatic suprasellar germinoma. After acute neurological deterioration, the patient underwent CT scanning that demonstrated ventriculomegaly. A shunt tap revealed no flow and negative opening pressure. Due to suspicion of proximal shunt malfunction, the comatose patient underwent immediate surgical exploration of the ventricle catheter, which was found to be patent. However, acute severe hypernatremia was diagnosed during the procedure. After correction of the electrolyte disturbances, the patient regained consciousness and made a good recovery. Although rare, the effects of acute severe hypernatremia on brain volume and ventricular size should be considered in the differential diagnosis of ventriculoperitoneal shunt failure. Source


Fuchs O.,University of Berne | Latzin P.,University of Berne | Thamrin C.,University of Berne | Stern G.,University of Berne | And 7 more authors.
European Respiratory Journal | Year: 2011

Despite association with lung growth and long-term respiratory morbidity, there is a lack of normative lung function data for unsedated infants conforming to latest European Respiratory Society/American Thoracic Society standards. Lung function was measured using an ultrasonic flow meter in 342 unsedated, healthy, term-born infants at a mean±SD age of 5.1±0.8 weeks during natural sleep according to the latest standards. Tidal breathing flow-volume loops (TBFVL) and exhaled nitric oxide (eNO) measurements were obtained from 100 regular breaths. We aimed for three acceptable measurements for multiple-breath washout and 5-10 acceptable interruption resistance (Rint) measurements. Acceptable measurements were obtained in ≤285 infants with high variability. Mean values were 7.48 mL·kg-1 (95% limits of agreement 4.95-10.0 mL·kg-1) for tidal volume, 14.3 ppb (2.6-26.1 ppb) for eNO, 23.9 mL·kg-1 (16.0-31.8 mL·kg-1) for functional residual capacity, 6.75 (5.63-7.87) for lung clearance index and 3.78 kPa·s·L-1 (1.14-6.42 kPa·s·L-1) for Rint. In males, TBFVL outcomes were associated with anthropometric parameters and in females, with maternal smoking during pregnancy, maternal asthma and Caesarean section. This large normative data set in unsedated infants offers reference values for future research and particularly for studies where sedation may put infants at risk. Furthermore, it highlights the impact of maternal and environmental risk factors on neonatal lung function. Copyright©ERS 2011. Source

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