Buenos Aires, Argentina

University of Belgrano

Buenos Aires, Argentina

The University of Belgrano is a private university established in 1964 and located in the Belgrano district of the city of Buenos Aires, Argentina.It has 9 Departments:Architecture and Urban PlanningLaw and Political ScienceEconomicsHumanitiesEngineering and Computer TechnologyAgricultural scienceLanguage and Foreign StudiesHealth scienceApplied scienceThe school operates 90.9 FM, a station featuring eclectic programming and daily BBC News broadcasts. Wikipedia.

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Olivera J.,CONICET | Challu L.,University of Belgrano | Penedo J.M.G.,CONICET | Roussos A.,CONICET
Psychotherapy | Year: 2017

There is no consensus among different therapeutic approaches on the process of termination when therapy does not have a prefixed duration. Moreover, both clinicians and researchers are still exploring decision making in the termination of treatment. The present study assessed former client's perspective of therapy termination in a nonprobabilistic sample from Buenos Aires, Argentina. Seventy-three semistructured interviews, lasting60 min each, were conducted with participants that had finished a therapeutic treatment or dropped out. They were asked about several aspects of therapy, including their experience of termination, specifically who decided to terminate, if there was agreement on termination or not, and their thoughts on the termination process. All interviews were transcribed and analyzed using an adaptation of Consensual Qualitative Research (CQR). Quantitative analyses were also conducted to examine associations between variables. Two main factors emerged from the analysis: client/therapist initiative on termination; and level of agreement between client and therapist regarding termination. Whereas nearly all (95%) of therapistinitiated termination cases agreed on termination, client-initiated termination cases could be sorted in agreed (49%) and disagreed (51%) terminations. Both therapist-initiated terminations and agreed upon terminations presented more categories of positive termination motives, better therapeutic bond, and higher overall satisfaction with treatment. Implications for research and clinical practice are discussed. © 2017 American Psychological Association.

The process of feline immunodeficiency virus (FIV) entry into its target cells is initiated by the association of the surface (SU) subunit of the viral envelope glycoprotein (Env) with the cellular receptors CD134 and CXCR4. This event is followed by the fusion of the viral and cellular membranes, which is mediated by the transmembrane (TM) subunit of Env. We and others have previously demonstrated that the V3 domain of the SU subunit of Env is essential for CXCR4 binding. Of note, there are two contiguous and highly conserved potential N-glycosylation sites (418NST420 and 422NLT424) located at the C-terminal side of the V3 domain. We therefore decided to study the relevance for Env functions of these N-glycosylation motifs and found that disruption of both of them by introducing the N418Q/N422Q double amino acid substitution drastically impairs Env processing into the SU and TM subunits. Moreover, the simultaneous mutation of these N-glycosylation sites prevents Env incorporation into virions and Env-mediated cell-to-cell fusion. Notably, a recombinant soluble version of the SU glycoprotein carrying the double amino acid replacement N418Q/N422Q at the V3 C-terminal side binds to CXCR4 with an efficiency similar to that of wild-type SU. © 2016, Springer-Verlag Wien.

Gonzalez S.A.,University of Belgrano | Paladino M.G.,University of Belgrano | Affranchino J.L.,University of Belgrano
Virology | Year: 2012

The feline immunodeficiency virus (FIV) envelope glycoprotein (Env) possesses a short cytoplasmic domain of 53 amino acids containing four highly conserved cysteines at Env positions 804, 811, 815 and 848. Since palmitoylation of transmembrane proteins occurs at or near the membrane anchor, we investigated whether cysteines 804, 811 and 815 are acylated and analyzed the relevance of these residues for Env functions. Replacement of cysteines 804, 811 and 815 individually or in combination by serine residues resulted in Env glycoproteins that were efficiently expressed and processed. However, mutations C804S and C811S reduced Env fusogenicity by 93% and 84%, respectively, compared with wild-type Env. By contrast, mutant C815S exhibited a fusogenic capacity representing 50% of the wild-type value. Remarkably, the double mutation C804S/C811S abrogated both Env fusion activity and Env incorporation into virions. Finally, by means of Click chemistry assays we demonstrated that the four FIV Env cytoplasmic cysteines are palmitoylated. © 2012 Elsevier Inc.

Barata-Vallejo S.,University of Buenos Aires | Postigo A.,University of Belgrano
Journal of Organic Chemistry | Year: 2010

Figure presented. Perfluoroalkyl-substituted compounds are regarded as important components of fluorophors and for the introduction of fluorous tags into organic substrates. Their syntheses in organic solvents are achieved through different methods, among which, the addition of perfluoroalkyl radicals to unsaturated bonds represents a convenient choice. On the other hand, intermolecular radical reactions in water have attracted the attention of synthetic chemists as a strategic route to carbon-carbon bond formation reactions. In this paper we undertook the intermolecular addition of perfluoroalkyl radicals on electron rich alkenes and alkenes with electron withdrawing groups in water, mediated by silyl radicals, and obtained perfluoroalkyl-substituted compounds in fairly good yields. The radical triggering events employed consist of the thermal decomposition of an azo compound and the dioxygen initiation. Our results indicate that for intermolecular carbon-carbon bond formation reactions mediated by (Me 3Si)3SiH, the decomposition of the azo compound 1,1′-azobis(cyclohexanecarbonitrile) (ACCN) is the best radical initiator. We also found that water exerts a relevant solvent effect on the rates of perfluoroalkyl radical additions onto double bonds and the H atom abstraction from the silane. Our account provides a versatile and convenient method to achieve perfluoroalkylation reactions of alkenes in water to render perfluoroalkylated alkanes as key intermediates in the synthesis of fluorophors and other fluorinated materials. This is the first report where perfluoroalkyl-substituted alkanes are synthesized through intermolecular radical carbon-carbon bond formation reactions in water, mediated by silyl radicals. © 2010 American Chemical Society.

Postigo A.,University of Belgrano | Nudelman N.S.,University of Buenos Aires
Journal of Physical Organic Chemistry | Year: 2010

The relevance of radical initiation methodologies for the classical hydrosilylation reactions of organic compounds bearing C-C multiple bonds is due to the need to come up with newer and more efficient methods to effect this reaction, on account of its applications on surface chemistry. In the past, when organic solventswere employed, thermal and photochemical methods for the chain initiation reaction have been documented (thermal and photochemical decompositions of azo compounds). We herein present the dioxygen-initiation technique of the classical radical hydrosilylation reaction of C-C triple bonds with tris(trimethylsilyl)silane ((Me3Si)3SiH) in water. This initiation technique is confronted with the photochemical radical initiation in the absence of a chemical radical precursor other than the silane and also confronted with the classical thermal initiation triggered by the decomposition of an azo compound, both performed in water. The radical-based dioxygen initiation methodology studied in water is shown to afford the highest Z:E stereoselective ratios of hydrosilylated alkenes. Copyright © 2010 John Wiley and Sons, Ltd.

Muller F.,University of Belgrano
Psychoanalytic Quarterly | Year: 2016

This paper describes the shift that appears to be taking place in contemporary psychoanalysis, as reflected among intersubjective approaches, from a monological conception of the self to a dialogical one. The monological self emphasizes the separation between mind, body, and external world, focusing on the representational and descriptive/referential function of language. In contrast, the dialogical self emphasizes practices, the permeable nature of relationships between subjects, and the constitutive function of language. This paper attempts to explain the growing emphasis on the dialogical self, understood from a theoretical, metatheoretical, and technical point of view, using contemporary intersubjective approaches to illustrate this shift. © 2016 The Psychoanalytic Quarterly, Inc.

Affranchino J.L.,University of Belgrano | Gonzalez S.A.,University of Belgrano
Virus Research | Year: 2010

The retroviral Gag protein is the only viral product that is necessary for the assembly of virions in mammalian cells. We have established an in vitro assembly system to study the assembly properties of purified feline immunodeficiency virus (FIV) Gag protein expressed in bacteria. Under fully defined conditions, the FIV Gag protein assembles into spherical particles of 33. nm in diameter which are morphologically similar to authentic immature particles, albeit smaller than virions. The in vitro assembly of FIV Gag into particles was found to be resistant to the addition of Triton X-100 and required the presence of RNA. Notably, we found that an amino acid substitution in the nucleocapsid domain of Gag that impairs RNA binding and blocks virion production in vivo, also abrogates Gag assembly in vitro. The development of an in vitro assembly system for FIV Gag protein will facilitate the study of the mechanisms by which this protein assembles into immature particles. © 2010 Elsevier B.V.

Gantman E.R.,University of Belgrano | Gantman E.R.,University of Buenos Aires
Scientometrics | Year: 2012

This paper examines the influence of economic, linguistic, and political factors in the scientific productivity of countries across selected scientific disciplines. Using a negative binomial regression model, I show that the effect of these determinants is contingent upon the scientific field under analysis. The only variable that exerts a positive and significant effect across all disciplines is the size of the economy. The linguistic variable only has a positive influence in the social sciences as well as in medicine and agricultural sciences. In addition, it is also demonstrated that the degree of political authoritarianism has a negative and statistically significant effect in some of the selected fields. © 2012 Akadémiai Kiadó, Budapest, Hungary.

Barata-Vallejo S.,University of Buenos Aires | Nudelman N.S.,University of Buenos Aires | Postigo A.,University of Belgrano
Current Organic Chemistry | Year: 2011

This account is focused on highlighting the recent advances on synthetically-useful organic reactions employing siliconcentered radicals in water, and presenting new reactions in water, mediated by silyl radicals. In doing so, several types of organic radical transformations will be discussed, such as reduction of organic halides utilizing non toxic organosilane reducing agents in water, transformation of azides into amines, synthesis of protecting silyl ethers in water, hydrosilylation reactions of carbon-carbon double and triple bonds, and radical cyclization reactions in water induced by silicon-centered radicals. More recently, intermolecular radical carboncarbon bond formation reactions mediated by silyl radicals have allowed the synthesis of perfluoroalkyl-substituted compounds in water, widening the scope for the syntheses of fluorophoes. These silicon radical-mediated chain reactions in water are initiated through different methods, among which, thermal, photochemical, and dioxygen initiations are reported to be the most successful methods in water. A versatile aspect of the radical methodology employed in water will be presented in terms of dealing with water-soluble and organic solvent- soluble substrates in these silicon radical-mediated reactions in water. In this regard, for an efficient chain process to take place in water, a chain carrier must be used when water-soluble substrates are employed, whereas organic solvent-soluble materials do not require a chain transporter when silyl radicals are used in water. © 2011 Bentham Science Publishers Ltd.

Affranchino J.L.,University of Belgrano | Gonzalez S.A.,University of Belgrano
Viruses | Year: 2014

The lentiviral envelope glycoproteins (Env) mediate virus entry by interacting with specific receptors present at the cell surface, thereby determining viral tropism and pathogenesis. Therefore, Env incorporation into the virions formed by assembly of the viral Gag polyprotein at the plasma membrane of the infected cells is a key step in the replication cycle of lentiviruses. Besides being useful models of human immunodeficiency virus (HIV) infections in humans and valuable tools for developing AIDS therapies and vaccines, simian and feline immunodeficiency viruses (SIV and FIV, respectively) are relevant animal retroviruses; the study of which provides important information on how lentiviral replication strategies have evolved. In this review, we discuss the molecular mechanisms underlying the incorporation of the SIV and FIV Env glycoproteins into viral particles. © 2014 by the authors; licensee MDPI, Basel, Switzerland.

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