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Lee C.,Karolinska Institutet | Wigren E.,Karolinska Institutet | Trcek J.,Karolinska Institutet | Trcek J.,University of Maribor | And 10 more authors.
Environmental Microbiology | Year: 2015

Pseudomonas aeruginosa is a highly successful nosocomial pathogen capable of causing a wide variety of infectionswithclone C strains most prevalentworldwide. In this study, we initially characterize a molecular mechanism of survival unique to clone C strains. We identified aP.aeruginosa clone C-specific genomic island (PACGI-1) that contains the highly expressed small heat shock protein sHsp20c, the founding member of a novel subclass of class B bacterial small heat shock proteins. sHsp20c and adjacent gene products are involved in resistance against heat shock.Heat stable sHsp20c is unconventionally expressed in stationary phase in a wide temperature range from 20 to 42°C. PurifiedsHsp20c has characteristic features of small heat shock protein class B as it is monodisperse, forms sphere-like 24-meric oligomers and exhibits significant chaperone activity. As theP.aeruginosaclone C population is significantly more heat shock resistant than genetically unrelated P.aeruginosa strains without sHsp20c, the horizontally acquired shsp20c operon might contribute to the survival of worldwide-distributed clone C strains. Copyright © 2015 Society for Applied Microbiology and John Wiley & Sons Ltd.


Khan H.,Gomal University | Farid Khan M.,Gomal University | Khan B.A.,Islamia University of Bahawalpur | Khan B.A.,Gomal University | And 4 more authors.
Biomedical Research | Year: 2012

Aluminium metal has important role in the field of medical sciences, but it has been found in various studies that Aluminium can cause numerous toxic effects. Studies have proven the strong affinity of the metalloelements for the sulfhydryl group (SH), present in reduced glutathione (GSH) and other biological molecules. In this context, the study about the possible interaction of Aluminium metal with glutathione in blood components was of interest, as an indication about the extent of Aluminium toxicity and the role of glutathione in the conjugation and detoxification of the metalloelement Aluminium metal. The concentration and time dependent effect of Aluminium metal on the level of GSH in plasma and Cytosolic Fraction in blood was investigated, following Ellman's method. It was found that Aluminium metal causes a decrease in the GSH level, which is more pronounced with increasing concentration of Aluminium metal and with time incubation as well. The observed effect GSH concentration may be presumably due to production of oxidized glutathione (GSSG) or then due to Aluminium-Glutathione (GS-Al) conjugate formation.

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