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Bucharest, Romania

University of Agronomic science and Veterinary Medicine in Bucharest, Romania, is a university which specializes in agriculture and veterinary medicine. Its campus is located on 59, Mărăşti Blvd. Wikipedia.

Vamanu E.,University of Agronomical Sciences and Veterinary Medicine
Molecules | Year: 2012

The antioxidant and antimicrobial potential of the ethanolic extract of Pleurotus ostreatus PQMZ91109 mycelium was determined based on inorganic and organic nitrogen sources in the culture medium. The presence of ammonium sulfate resulted in a greater accumulation of bioactive compounds compared with the organic ones. This finding was also confirmed by the low values of the ascertained EC 50 and minimum inhibitory concentration (MICs). Among the organic sources, peptone followed by corn extract, led to a more important radical-scavenging activity. The extracts selectively inhibited the tested strains, mainly the two of the genus Candida, at an MIC value of 1.25 mg/mL. The antioxidant potential was evaluated by the inhibition capacity of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, β-carotene-linoleic acid, which is the reducing power. In addition, the quantity of the compounds with antioxidant effects confirmed the data obtained, they being present in the extracts. © 2012 by the authors.

Vamanu E.,University of Agronomical Sciences and Veterinary Medicine | Nita S.,Romanian National Institute for Chemical Pharmaceutical Research and Development
BioMed Research International | Year: 2013

Boletus edulis is a wild edible mushroom habitually consumed by rural populations. Ethanolic and methanolic extracts was obtained in cold and hot water from dried fruit bodies. The antioxidant activity of freeze-dried extracts from B. edulis were investigated using free radicals scavenging activity, reducing power, metal chelating effect, inhibition of lipid peroxidation, and the identification of antioxidant compounds. The levels of different compounds with antioxidant properties were higher in alcoholic extracts compared with aqueous extracts. Rosmarinic acid was the major phenolic compound, it being identified in a concentration between 7±0.23 and 56±0.15 mg/100 g extract. A positive correlation between the content of total phenols, flavonoids, anthocyanins, and tocopherols, and the antioxidant capacity of the extracts was determined. The results showed that the ethanolic extract of Romanian wild mushroom B. edulis represents a natural source of functional compounds. © 2013 Emanuel Vamanu and Sultana Nita.

Vamanu E.,University of Agronomical Sciences and Veterinary Medicine
Revista de Chimie | Year: 2013

The purpose of this study was to determine the antioxidant activities of four different extracts from the mushroom Pleurotus ostreatus available in Romanian markets. Antioxidant activity was calculated by the reducing power assay, the scavenging effect on free radicals, inhibition of lipid peroxidation and chelating effects on ferrous ions. Ethanolic extract presented the highest free radicals scavenging and metal chelating activities. Thus, significant positive correlations proved that the antioxidant effects of ethanolic extract were a result of the presence of the phenolic and flavonoidic compounds. However, the ability to inhibit lipid peroxidation depended on the solvent: methanol > ethanol > cold water > hot water. In the four extracts, different types of molecules with antioxidant properties were determined.

Vamanu E.,University of Agronomical Sciences and Veterinary Medicine
Revista de Chimie | Year: 2013

The purpose of this study is to determine the antioxidant activity of fluidized bed extracts from Betula verucosa leaves. Extractions were performed using ethanol and methanol, both at a concentration of 70%, and the phytochemical composition was determined for each type of extract after lyophilization. Evaluation of the antioxidant potential was achieved by free radicals scavenging activity, by the reducing power, the chelating effect on ferrous ions, and inhibition of human erythrocyte hemolysis. The obtained results showed that both extracts had a strong antioxidant activity, and also possessed human erythrocyte membrane protection against oxidative action of 2,2 '-azo-bis (2-amidinopropane) dihydrochloride (AAPH), mainly for the ethanol lyophilisate. In the case of the phytochemical analysis, significant contents of phenolics, flavonoids, proanthocyanidins, saponins, alkaloids, and vitamin C were determined. A significant correlation between the antioxidant properties of lyophilized extracts, the phenolic component, and the content of ascorbic acid were also calculated.

Pop A.,University of Agronomical Sciences and Veterinary Medicine
Current pharmaceutical biotechnology | Year: 2015

Antibiotic resistance is a major problem in current contemporary medicine and it has become a major concern of the 21st century. New resistance mechanisms developed by microorganisms spread greatly, threatening the ability to treat numerous infectious diseases, and increasing the number of nosocomial infections. Besides the role in immunology and glycobiology where they are used as hemaglutinine and identification of complex carbohydrates and glycoconjugates, lectins proved to mediate diversified biological functions like cytotoxicity, complement activation, cell-to-cell and host-pathogen communications, innate immune response, and cell-to-cell signalling. Recently, great interest has been developed for the research and applications of lectins in agriculture and medicine due to their antiparasitic and antimicrobial potentials. This review focuses on the recent data regarding the antimicrobial and antiparasitic activities of lectins, by presenting the role of lectins in host-pathogen interaction and also the cytotoxic effects on microorganisms and parasites. Identification and characterisation of new lectins with antimicrobial activity could serve as a natural alternative for the treatment of infections caused by antibiotic-resistant microorganisms and parasites.

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