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Aldea N.,Romanian Institute of Isotopic And Molecular Technology | Kolipaka K.L.,Leibniz Institute for Plasma Science and Technology | Rednic V.,Romanian Institute of Isotopic And Molecular Technology | Matei F.,University of Agriculture Science and Veterinary Medicine
Journal of Optoelectronics and Advanced Materials | Year: 2011

A new version of Fourier analysis of XANES and EXAFS data obtained using synchrotron radiation, a self-contained Fortran program-package and Gnuplot with graphical data interface facilities that permits users a simple manipulation is described. By the direct dialog between the user and the Gnuplot supports, based on XANES and EXAFS spectra, there is the possibility to obtain the electronic and structural parameters. An improved procedure is used to extract more information from XANES spectra by second derivative analysis and electronic transitions distribution associated for each edge. Another purpose of the program package is based on EXAFS measurements analysis, to obtain the structural parameters: Rj - distance from the central absorbing atom to atoms in the j th coordination shell, N j - number of atoms in the j th shell, σ j - root mean square deviation of the interatomic distance about R j, λ j - mean free path for inelastic scattering, A j - backscattering amplitude envelope function and O j(k) - phase shift. Additional the principal component analysis allows determining the number of primary components in a set of experimental XANES or EXAFS spectra. The new computer package program was tested on different systems: supported metal catalysts, oxides and other materials. Source

Bernhardsson C.,Swedish University of Agricultural Sciences | Floran V.,Swedish University of Agricultural Sciences | Floran V.,University of Agriculture Science and Veterinary Medicine | Ganea S.L.,Swedish University of Agricultural Sciences | And 2 more authors.
Forest Ecology and Management | Year: 2016

Owing to the fact that mitochondrial, chloroplast and nuclear genomes are differently affected by evolutionary forces, the comparative study of all three genomes in combination with paleobotanical evidences allows for a more accurate interpretation of present genetic make-up. In this study, we analyzed the nad1-nad7 mitotype, 14 cpSSR loci and 10 nSSR loci in 13 Romanian Scots pine (Pinus sylvestris L.) populations from the Romanian Carpathians and two Hungarian populations. Previous analysis of the macrofossil records supports the presence of Scots pine and Swiss stone pine (Pinus cembra L.) during the glacial period in Hungary and Romania. For a better image into the population structure in the studied area we also included in the data analysis two outgroup populations (north and south of Sweden) of known post-glacial origin, which resulted in a more defined genetic structure. Our study reveals that relatively high genetic diversity is preserved at all the Romanian and Hungarian populations, with no distinction among naturally regenerated, managed autochthonous and introduced populations. Furthermore, genetic differentiation analysis fails to distinguish the two introduced populations from the Romanian ones. AMOVA clustering is congruent with the paleobotanical-based evidences of contraction of Scots pine range since the Holocene to its present fragmented distribution. © 2015 Elsevier B.V. Source

Dascalu C.,Bioterra University | Bogdan A.T.,Romanian Academy of Sciences | Sonea A.,University of Veterinary and Animal Sciences | Tapaloaga P.R.,University of Veterinary and Animal Sciences | And 8 more authors.
Recent Researches in Energy and Environment - 6th IASME / WSEAS International Conference on Energy and Environment, EE'11 | Year: 2011

The increasing of cattle number from livestock ecosystems is a major objective in achieving feeding security of the human population. By the same time, the sustainable development of livestock is tightly linked to the protection, conservation and development of human useful animal species biodiversity. In this study are presented the comparative results from specialty literature about cows breading, depending on the exploitation technology and factors that influence milk production. By the same time, comparisons are made between the mode of cows maintenance at national and international level with existing conditions in Vrancea County in Romania, regarding the applicability of possible strategies of reorganization of exploitation technologies for milk cattle, according to race, ecological zone, size of holdings and form of property. Source

Bosca A.B.,University of Medicine and Pharmacy, Cluj-Napoca | Miclaus V.,University of Agriculture Science and Veterinary Medicine | Ratiu C.,University of Oradea | Melincovici C.,University of Medicine and Pharmacy, Cluj-Napoca
Annals of the Romanian Society for Cell Biology | Year: 2012

Periodontitis is a bacteria-induced chronic inflammation affecting the tooth-supporting structures. A major challenge in clinical periodontitis is to find a novel diagnostic tool for an objective evaluation of the periodontal status. The aim of this study is to assess the value of salivary Matrix metalloproteinase (MMP)-8, a neutrophil-derived proteolytic enzyme, as an indicator of the severity of periodontal tissues destructions. The study included 11 chronic periodontitis patients and 10 healthy controls. Clinical parameters including periodontal index (PI), bleeding on probing index (BPI), probing depth (PD) and clinical attachment level (CAL) were recorded, and saliva samples were collected. MMP-8 salivary levels were measured using an ELISA quantitative colorimetric assay. The chronic periodontitis patients underwent initial therapy (scaling and root planning) followed by periodontal surgery. The soft tissue wall of the pathologic periodontal pocket was excised and examined microscopically. Statistical analysis (the independent samples test and Pearson correlation coefficient) was employed to compare the clinical parameters with MMP-8 salivary levels. We also compared these data with the histopathological findings. Our results indicated that there was a correlation between the periodontal status evaluated using clinical parameters, MMP-8 salivary levels and the histopathological changes. Salivary MMP-8 can be taken into consideration as a biomarker of periodontitis and could be used as a valuable indicator of health and pathologic process. Source

Dulf F.V.,University of Agriculture Science and Veterinary Medicine | Unguresan M.-L.,Technical University of Cluj Napoca | Vodnar D.C.,University of Agriculture Science and Veterinary Medicine | Socaciu C.,University of Agriculture Science and Veterinary Medicine
Notulae Botanicae Horti Agrobotanici Cluj-Napoca | Year: 2010

The unsaponifiable lipid fraction of plant-based foods is a potential source of bioactive components such as phytosterols, triterpenoids, carotenoids, tocopherols and various hydrocarbons. The free and esterified sterol concentrations in four Romanian edible oils (corn germ, wheat germ, sweet almond and grape seed oil) were determined, including individual values for β-sitosterol, campesterol, stigmasterol, Δ5-avenasterol, sitostanol, campestanol, and cholesterol. Free and esterified sterols were separated by solid-phase extraction (SPE), saponified, and analyzed as trimethylsilyl ether derivatives using gas-chromatography (GC) with flame ionization detector (FID). Differences in total sterol content and the proportion of esterified (ES) and free sterols (FS) were evident for studied oil samples. In general, β-sitosterol was the most prevalent phytosterol, ranging in concentration from 158.3 mg/100 g in grape seed oil to 478.5 mg/100 g in corn germ oil. Only in these two vegetable oil, we identified trace amount of cholesterol (<3 mg/100g). The total sterol concentrations ranged from 199.9 mg/100g (sweet almond oil) to 745.2 mg/100 g (corn germ oil). In corn germ and wheat germ oil, the dominant form of sterols was the esterified one (60.7% ES and 55.6% ES, respectively, of total sterols). This study consolidates the view that vegetable oils are good natural sources of phytosterols. The analyses of these components provide rich information about the identity and quality of vegetable oils. The corn germ and wheat germ oils proved to be the richest sources in phytosterols, being recommended as functional oils. Source

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