Khan H.,University of Agriculture, Peshawar |
Yousaf M.S.,University of Veterinary and Animal Sciences |
Ahmad I.,University of Veterinary and Animal Sciences |
Khan D.,University of Agriculture, Peshawar |
And 4 more authors.
Life Science Journal | Year: 2014
The current investigation was carried out to clarify whether the addition of antioxidant Alpha Lipoic Acid (ALA) increases the quality of cryopreserved Nili Ravi buffalo semen. Semen from five healthy Nili-Ravi buffalo bulls was collected by artificial vagina and subjected to the different inclusion levels of ALA @ 0.5mM, 1.mM, 2.mM, 3.mM, and 4.mM respectively. Experiments were executed for Post thawed semen analysis including spermatozoa motility, viability, plasma membrane integrity and acrosomal integrity. Our result indicated that Spermatozoa motility and viability was significantly higher (P < 0.05) at lower amount of alpha lipoic acid at 0.5 and 1.mM whereas the spermatozoa acrosomal integrity was significantly higher (P < 0.05) at 0.5mM. On the other hand, the lowest spermatozoa acrosomal integrity was observed with increasing concentration of alpha lipoic acid at 2.0, 3.0 and 4.mM. Our result further demonstrated that plasma membrane integrity was higher at 0.5 mM (P < 0.05). Based on the finding of current study, it is evident that the Antioxidant Alpha Lipoic Acid could be used for enhancing quality of the post thawed buffalo spermatozoa at lower concentration (0.5mM).