Rey-Rubio R.,University of a Corana |
Moreda-Pineiro J.,University of a Corana |
Alonso-Rodriguez E.,University of a Corana |
Lopez-Mahia P.,University of a Corana |
And 4 more authors.
Atomic Spectroscopy | Year: 2012
A fast, simple, automated, and "green" method, using pressurized hot water extraction, has been developed for the determination of total As, Ba, Cd, Cr, Mn, Ni, Pb, and Zn in edible seaweeds (Dulse, Nori, Kombu, Wakame, Sea Spaghetti, Sea Lettuce, and Furbelows). Pressurized hot water modified with acetic acid (150 mM) was tested as a solvent to extract these metals from several seaweed samples. Two-level factorial designs were used to optimize the pressurized hot water extraction (PHWE) procedure. The effects of several parameters were evaluated using a Plackett-Burman 29*3/32 experimental design, type III resolution, and four degrees of freedom. The significant parameters (extraction temperature and extraction pressure) obtained were optimized by using 22 + star central composite design. The accuracy of the methods was verified by analyzing BCR249 Sea Lettuce. Detection and quantification limits in the range of 0.02-2.2 μg g1 and 0.05-7.3 μg g1were achieved, respectively. The developed method was applied to several Atlantic seaweeds harvested in the Galician coast (Northwestern Spain).