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Neto A.G.B.,Federal University of Pernambuco | Pestana-Calsa M.C.,Federal University of Pernambuco | Pestana-Calsa M.C.,University Mauricio Of Nassau | de Morais Jr. M.A.,Federal University of Pernambuco | Calsa Jr. T.,Federal University of Pernambuco
Journal of Proteomics | Year: 2014

Dekkera bruxellensis is an industrially relevant yeast, especially in bioethanol production. The capacity of D. bruxellensis to assimilate nitrate can confer advantages of this yeast over Saccharomyces cerevisiae at industrial conditions. In the present work we present the consequences of nitrate assimilation, using ammonium as reference, to the proteomics of D. bruxellensis. Thirty-four protein spots were overproduced in nitrate medium and were identified by MS-TOF/TOF analysis and were putatively identified by using local Mascot software. Apart from the overexpression of genes of nitrate metabolism, ATP synthesis and PPP and TCA pathways previously reported, cultivation on nitrate induced overproduction of glycolytic enzymes, which corroborate the high energy demand and NADH availability for nitrate assimilation. Overproduction of alcohol dehydrogenase (Adh) protein was also observed. Proteomic profile of D. bruxellensis cultivated in nitrate and described in the present work agrees with the hypothesis of metabolic flux regulation, making available the energy in the form of NADH to support nitrate assimilation. This work contributes with an initial picture of proteins presenting differential accumulation in industrial contaminant yeast, in strict association with possible metabolic responses to nitrate as sole nitrogen source in cultivation medium. © 2014 Elsevier B.V. Source

Bello M.,Federal University of Piaui | Sousa M.C.,Federal University of Paraiba | Neto G.,Federal University of Paraiba | Oliveira L.,University Mauricio Of Nassau | And 3 more authors.
Journal of Human Kinetics | Year: 2014

The aim of this study was to evaluate the impact of a community-based exercise program on bone mineral density and body composition in postmenopausal women with pre-diabetes and type 2 diabetes. Twenty postmenopausal women (aged 61.3 ± 6.0 years) with pre-diabetes and type 2 diabetes were randomly assigned to a community-based exercise program group (n=10) or a control group (n=10). The community-based exercise program was multicomponent, three days per week for 32 weeks, and included walking, resistance and aquatic exercises. Body composition and bone mineral density were measured pre and post-training by dual X-ray absorptiometry. In the exercise group significant increases were found in the ward's triangle bone mineral density (+7.8%, p=0.043), and in fat-free mass (+2.4%, p=0.018). The findings suggest that regular multicomponent training is effective in preventing osteoporosis and sarcopenia among postmenopausal women with pre-diabetes and type 2 diabetes. © 2014 by Marieni Bello. Source

dos Santos R.C.,Laboratorio Of Biotecnologia | Ferraz G.M.G.,University Mauricio Of Nassau | de Albuquerque M.B.,Federal University of Paraiba | de Lima L.M.,Laboratorio Of Biotecnologia | And 2 more authors.
Acta Botanica Brasilica | Year: 2014

The temporal expression of gene sor1 and the inhibitory effect of Sorghum bicolor L. Moench against weeds were studied by semiquantitative polymerase chain reaction and intercropping management, respectively. To quantify sor1 expression, seeds were sown in pots and RNA was collected from the roots at 5, 10, 15, 20 and 30 days after emergence (DAE). In the inhibition assay, cotton and three weeds were evaluated during single cropping and during intercropping with S. bicolor. The assay was completely randomized, with eight replications. We found early expression of sor1 in most S. bicolor accessions by 5 DAE, and a gradual reduction thereafter. Only one of the accessions showed sor1 expression up to 30 DAE. In the inhibition assay, the most significant effects were related to the dry matter production (shoots and roots) of the weeds Cenchrus echinatus and Cynodon dactylon. The intercropping of cotton and S. bicolor had no apparent deleterious effects. © 2014, Sociedade Botanica do Brasil. All rights reserved. Source

Cardoso G.A.,Regional University of Cariri | Silva A.S.,Federal University of Paraiba | Araujo De Souza A.,Federal University of Paraiba | Pereira Dos Santos M.A.,Federal University of Piaui | And 3 more authors.
Journal of Human Kinetics | Year: 2014

This study investigated the chronic and acute influence of resistance exercise on blood pressure in women with metabolic syndrome before and after climacteric. Twenty sedentary women, nine non-menopausal (RNM) and 11 menopausal (RM), performed training for 12 weeks. Meanwhile, 23 controls, 11 not menopausal (CNM) and 12 menopausal (CM), remained sedentary. Blood pressure was measured before and after the training period in conditions of rest and after a session of exercise. Training promoted variations in blood pressure at rest from 116±13 to 118±10 mmHg (p=0.73) and from 128±12 mmHg to 120±11mmHg (p=0.12) in RNM and RM, respectively. CNM and CM varied from 115±11 to 116±12 mmHg (p=0.9) and from 115±14 mmHg to 116±13 mmHg (p=0.74). Blood pressure values in one acute session did not differ between groups (p>0.05). Resistance training did not improve blood pressure in women with metabolic syndrome, regardless of climacteric. © 2014 by Alexandre Sérgio Silva. Source

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