Neto A.G.B.,Federal University of Pernambuco |
Pestana-Calsa M.C.,Federal University of Pernambuco |
Pestana-Calsa M.C.,University Mauricio Of Nassau |
de Morais Jr. M.A.,Federal University of Pernambuco |
Calsa Jr. T.,Federal University of Pernambuco
Journal of Proteomics | Year: 2014
Dekkera bruxellensis is an industrially relevant yeast, especially in bioethanol production. The capacity of D. bruxellensis to assimilate nitrate can confer advantages of this yeast over Saccharomyces cerevisiae at industrial conditions. In the present work we present the consequences of nitrate assimilation, using ammonium as reference, to the proteomics of D. bruxellensis. Thirty-four protein spots were overproduced in nitrate medium and were identified by MS-TOF/TOF analysis and were putatively identified by using local Mascot software. Apart from the overexpression of genes of nitrate metabolism, ATP synthesis and PPP and TCA pathways previously reported, cultivation on nitrate induced overproduction of glycolytic enzymes, which corroborate the high energy demand and NADH availability for nitrate assimilation. Overproduction of alcohol dehydrogenase (Adh) protein was also observed. Proteomic profile of D. bruxellensis cultivated in nitrate and described in the present work agrees with the hypothesis of metabolic flux regulation, making available the energy in the form of NADH to support nitrate assimilation. This work contributes with an initial picture of proteins presenting differential accumulation in industrial contaminant yeast, in strict association with possible metabolic responses to nitrate as sole nitrogen source in cultivation medium. © 2014 Elsevier B.V.
Bello M.,Federal University of Piauí |
Sousa M.C.,Federal University of Paraiba |
Neto G.,Federal University of Paraiba |
Oliveira L.,University Mauricio Of Nassau |
And 3 more authors.
Journal of Human Kinetics | Year: 2014
The aim of this study was to evaluate the impact of a community-based exercise program on bone mineral density and body composition in postmenopausal women with pre-diabetes and type 2 diabetes. Twenty postmenopausal women (aged 61.3 ± 6.0 years) with pre-diabetes and type 2 diabetes were randomly assigned to a community-based exercise program group (n=10) or a control group (n=10). The community-based exercise program was multicomponent, three days per week for 32 weeks, and included walking, resistance and aquatic exercises. Body composition and bone mineral density were measured pre and post-training by dual X-ray absorptiometry. In the exercise group significant increases were found in the ward's triangle bone mineral density (+7.8%, p=0.043), and in fat-free mass (+2.4%, p=0.018). The findings suggest that regular multicomponent training is effective in preventing osteoporosis and sarcopenia among postmenopausal women with pre-diabetes and type 2 diabetes. © 2014 by Marieni Bello.
Cardoso G.A.,Regional University of Cariri |
Silva A.S.,Federal University of Paraiba |
Araujo De Souza A.,Federal University of Paraiba |
Pereira Dos Santos M.A.,Federal University of Piauí |
And 3 more authors.
Journal of Human Kinetics | Year: 2014
This study investigated the chronic and acute influence of resistance exercise on blood pressure in women with metabolic syndrome before and after climacteric. Twenty sedentary women, nine non-menopausal (RNM) and 11 menopausal (RM), performed training for 12 weeks. Meanwhile, 23 controls, 11 not menopausal (CNM) and 12 menopausal (CM), remained sedentary. Blood pressure was measured before and after the training period in conditions of rest and after a session of exercise. Training promoted variations in blood pressure at rest from 116±13 to 118±10 mmHg (p=0.73) and from 128±12 mmHg to 120±11mmHg (p=0.12) in RNM and RM, respectively. CNM and CM varied from 115±11 to 116±12 mmHg (p=0.9) and from 115±14 mmHg to 116±13 mmHg (p=0.74). Blood pressure values in one acute session did not differ between groups (p>0.05). Resistance training did not improve blood pressure in women with metabolic syndrome, regardless of climacteric. © 2014 by Alexandre Sérgio Silva.
PubMed | Regional University of Cariri, Federal University of Piauí, University Mauricio Of Nassau, Federal University of Paraiba and Royal University
Type: | Journal: Journal of human kinetics | Year: 2015
This study investigated the chronic and acute influence of resistance exercise on blood pressure in women with metabolic syndrome before and after climacteric. Twenty sedentary women, nine non-menopausal (RNM) and 11 menopausal (RM), performed training for 12 weeks. Meanwhile, 23 controls, 11 not menopausal (CNM) and 12 menopausal (CM), remained sedentary. Blood pressure was measured before and after the training period in conditions of rest and after a session of exercise. Training promoted variations in blood pressure at rest from 11613 to 11810 mmHg (p=0.73) and from 12812 mmHg to 12011mmHg (p=0.12) in RNM and RM, respectively. CNM and CM varied from 11511 to 11612 mmHg (p=0.9) and from 11514 mmHg to 11613 mmHg (p=0.74). Blood pressure values in one acute session did not differ between groups (p>0.05). Resistance training did not improve blood pressure in women with metabolic syndrome, regardless of climacteric.
Gomes I.C.,University Mauricio of Nassau |
Dos Santos V.R.,Paulista University |
Christofaro D.G.D.,Paulista University |
Fernandes R.A.,Paulista University |
And 2 more authors.
Medicina (Brazil) | Year: 2016
Study design: Cross-sectional study. Objective: To analyze the presence of cardiovascular risk factors (CRFs) according to the pattern of body fat distribution, in Brazilians aged 80 years or over. Methods: The sample consisted of 113 subjects, of 83.4 years, of both sexes. The percentages of total and abdominal body fat, hypertension and lipid profile were used for characterization of CRFs. The chisquare test was used to assess proportions of CRFs and the Mann-Whitney test was used to compare the results of adiposity. Results: Eutrophic subjects presented lower triglycerides (p=0.017), total cholesterol (p=0.001) and prevalence of both hypertension (p=0.003) and hypertriglyceridemia (p=0.007). Subjects with higher abdominal adiposity presented higher total cholesterol (p=0.026) and prevalence of hypertriglyceridemia (p=0.011) and hypercholesterolemia (p=0.026) than those with no excess abdominal adiposity. Those with excess fat (total, abdominal or both), with the exception of glucose (p=0.877), presented a high prevalence of CRFs. Those with both types of excess fat presented differences, with a higher prevalence level when compared to those with only one or neither of the excesses. Conclusion: Obesity, whether abdominal or total, is similarly associated with CRFs in subjects aged 80 years and over.
PubMed | Paulista University and University Mauricio of Nassau
Type: | Journal: Diabetes & metabolic syndrome | Year: 2016
To analyze the lipid profile of older people aged 80 years according to body composition (high fat mass, low muscle mass and both).The sample consisted of 113 older people aged 80 years. The assessment of body composition was made using Dual Energy X-ray Absorptiometry (DXA) and the lipid profile analysis using an enzymatic colorimetric kit. We used Analysis of Variance (ANOVA) test to compare the mean of lipid profile according to body composition and were constructed logistic regression models to verify the association between these two variables.It was found that older people with high fat had higher mean values of TG compared to normal and low muscle mass group. Older people with low muscle mass showed mean values of LDL-c lower than other groups. It was observed that older people with high fat is more likely to have (OR 2.70; 95%CI 1.14-6.37) high blood concentration of TG.Thus, it appears that high fat is related to the high blood concentration of TG in older people aged 80 years, especially those with Asian origin and diabetes besides those with low muscle mass shows lower mean values of LDL-c.
dos Santos R.C.,Laboratorio Of Biotecnologia |
Ferraz G.M.G.,University Mauricio Of Nassau |
de Albuquerque M.B.,Federal University of Paraiba |
de Lima L.M.,Laboratorio Of Biotecnologia |
And 2 more authors.
Acta Botanica Brasilica | Year: 2014
The temporal expression of gene sor1 and the inhibitory effect of Sorghum bicolor L. Moench against weeds were studied by semiquantitative polymerase chain reaction and intercropping management, respectively. To quantify sor1 expression, seeds were sown in pots and RNA was collected from the roots at 5, 10, 15, 20 and 30 days after emergence (DAE). In the inhibition assay, cotton and three weeds were evaluated during single cropping and during intercropping with S. bicolor. The assay was completely randomized, with eight replications. We found early expression of sor1 in most S. bicolor accessions by 5 DAE, and a gradual reduction thereafter. Only one of the accessions showed sor1 expression up to 30 DAE. In the inhibition assay, the most significant effects were related to the dry matter production (shoots and roots) of the weeds Cenchrus echinatus and Cynodon dactylon. The intercropping of cotton and S. bicolor had no apparent deleterious effects. © 2014, Sociedade Botanica do Brasil. All rights reserved.